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Chlorophyll Fluorescence and Growth Response of Three South Korea Native Fern Species under In-door Light Intensity

  • Kyungtae Park;Bo Kook Jang;Cheol Hee Lee;Sang Yeob Lee;Ju Sung Cho
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2020.08a
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    • pp.63-63
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    • 2020
  • This study investigated the growth of native ferns under indoor light intensities to identify the introduction possibility as in-door ornamental plants. Three evergreen perennial fern species used in this experiment were Coniogramme japonica (Thunb.) Diels, Woodwardia japonica (L. f.) Sm., and Cyclosorus acuminatus (Houtt.) Nakai ex H. Itô. The light intensities were adjusted to 10, 50, 100 and 200 PPFD (µmol·m-2·s-1) based on the measurement of the various indoor light quantities. The experiment was conducted for a total of 8 weeks, and the light period (12/12h), temperature (25±1℃), and humidity (55±3%) were maintained during the experiment. The control plant group was grown in glass greenhouse for the same period. As the result of the study, in door C. japonica showed better growth under light intensities of 100, 200 PPFD. However, withering of the plants were observed under all light intensities except the control, which causes an ornamental value decrease. This seems to be related to the increase of DIo/RC value in chlorophyll fluorescence parameters. In the W. japonica growth data, the plant height was not significantly different from the control but, the leaf number decreased more than a two-fold. Also, the formed leaves turned brown and showed a poor growth and SPAD value at 200 PPFD had decreased significantly. Growth data of C. acuminatus was not significantly different with the control at all light intensities however, withering was observed at 100 and 200 PPFD. In chlorophyll fluorescence parameters, significant decrease in Pi_Abs and increase in DIo/RC value at 200 PPFD impose that stress caused by the intense light might be the reason of the withering of the plants.

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A Study on Establishment of Technical Guideline of the Installation and Operation for the Efficient Bio-gasification Facility of Pig Manure and Food Waste(II): - Results of the Precision Monitoring - (가축분뇨 병합처리 바이오가스화를 위한 설계 및 운전 기술지침 마련 연구(II) - 정밀모니터링 결과 중심으로 -)

  • Lee, Dongjin;Moon, HeeSung;Son, Jihwan;Bae, Jisu
    • Journal of the Korea Organic Resources Recycling Association
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    • v.25 no.3
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    • pp.91-98
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    • 2017
  • The purpose of this study is to provide a design and operation technical guideline for meeting the appropriate design criteria to bio-gasification facilities treating organic wastes. 9 anaerobic digestion facilities which is normally operated during the field survey and 14 livestock manure farms were selected for precision investigation. the physicochemical analysis was performed on the moisture and organic contents, nutrients composition (carbohydrate, fat, protein), volatile fatty acids (VFAs), and nitrogen, etc. Volatile solids (VS) of organic wastes brought into the bio-gasification facilities were 2.81 % (animal manure only) and 5.92 % (animal manure+food waste), respectively. Total solids (TS) reults of samples from livestock farms were 5.6 % in piglets and 11~13 % in other kinds of breeding pigs. The actual methane yield based on nutrients contents was estimated to $0.36Sm^3CH_4/kgVS$ which is equivalent to 72 % of theoretical methane yield value. The optimum mixing ratio depending on the effect of the combined bio-gasification was obtained through the continuous stirred-tank reactor (CSTR) which is operated at different mixing ratio of swine manure and food waste leachate. The range of swine manure and food waste leachate from 60:40 to 40:60 were adequate to the appropriate conditions of anaerobic digestion; less than 100 gTS/, more than alkalinity of 1 gCaCO3/L, C/N ratio 12.0~30.0, etc.

Fluvial Deposits Distributed along the Seomjin River (섬진강 유역의 하성 퇴적층에 관한 연구)

  • You, Hoen-Su;Cho, Seok-Hee;Koh, Yeong-Koo
    • Journal of the Korean earth science society
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    • v.21 no.2
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    • pp.174-187
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    • 2000
  • The Quaternary coarse-grained sandy sediments are distributed along the channels of Seomjin River. The fluvial sediments were sedimentologically studied in horizontal and vertical distributions. To analyze depositional environments and facies changes in the sediments, sediment sampling from river mouth to upper stream and desctriptive approaches to the sediment profiles outcropped near Kurye were carried out. The sediments along the stream lines of the river are assigned to very coarse to coarse sand in grain size. The sediment grains are widely scattered in sorting and moderately sorted in average. For skewness and kurtosis, the sediments ranges from very fine to very coarse skewed and from very lepto-kurtic to extremelyl epto-kurtic states, respectively. The sediments are divided into slightly gravelly sand, gravelly sand and sandy gravel in sediment type. The pain shape in the sandy sediments are dominant in equant and tabular forms showing wide varieties. The sandy sediments are mostly poorly sorted and are highly variable in surface texture with SEM. Some smaller grains in the sediments ordinarily show polished surfaces. Of those grains, quartz ones are commonly angular to surounded. On the basis of facies changes and sedimentary structures, outcropped fluvial sediment profiles in Kurye are classified into xGyS, mGyS, gGyS, xSM, xS, mS, mGyM, IgM in facies. These eight facies are reformed as facies assemblage I and ll. The facies assemblage I and II are interpreted as the products of the channel deposits in braided stream and flood plain ones besides channels, respectively. The change facies assemblage I with facies assemblage ll imply that depositional environments hadbeen migrated from braied sream to flood plain ones.

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Studies on Food Preservation by Controlling Water Activity 1. Measurement of Sorption Isotherm of Dried Filefish Muscle by Equilibration in Dynamic Stream of Conditioned Air (식품보장과 수분활성에 관한 연구 1. 조절기류에 의한 건조말쥐치육의 등온흡습곡선의 측정)

  • HAN Bong-Ho;CHOI Soo-Il
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.14 no.4
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    • pp.189-193
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    • 1981
  • An apparatus for continuous measurements of sorption isotherm of dried food was manufactured to shorten the time required for equilibration. The apparatus was so designed that the temperature, air velocity and relative humidity in the experimental chamber could be controlled. The use of dynamic stream of conditioned air with a velocity of 0.2m/sec, instead of static atmosphere, allowed a faster equilibration of dried filefish muscle at $25^{\circ}C$. The mean time necessary for the equilibration of dried filefish muscle at the water activity of a given state to a higher water activity was about 45 hours. The monolayer moisture content of dried filefish muscle calculated from BET-equation was 0.092 kg water /kg dry matter at $25^{\circ}C$.

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Optimization of Treatment Concentration and Screening of Exogenous Plant Growth Regulators for Improvement of Salidroside Yield in Rhodiola sachalinensis A. Bor Cell Suspension Cultures (참돌꽃 (Rhodiola sachalinensis A. Bor) 현탁세포배양에서 Salidroside 생산을 위한 외재 호르몬의 영향 및 처리 농도의 최적화)

  • Choi Hye-Jin;Kim Su-Jeong;Hwang Baik;Ahn Jun-Cheul
    • Journal of Plant Biotechnology
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    • v.32 no.2
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    • pp.105-109
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    • 2005
  • To enhance salidroside productivity from a cell suspension cultures of Rhodiola sachalinensis, various combinations of auxin (NAA) and cytokinins (BA, Kinetin) concentration and addition of $GA_3$, TDZ, zeatin, spermine and spermidine at a hormone combination to be established were examined. NAA/BA combination is superior to NAA/kinetin combination in biomass and salidroside content. Maximum salidroside production ($64.6{\pm}8.9\;mg/L$ medium) was obtained from $2B_5$ medium with 1 mg/L NAA and 5 mg/L BA. The adding of $GA_3$ ($0.01\;mg/L{\sim}5\;mg/L$) was beneficial for salidroside accumulation and the highest productivity of salidroside, $90.3{\pm}8.34\;mg/L$, was obtained from $2B_5$ medium supplemented with 1 mg/L NAA, 5 mg/L BA and 0.1 mg/L $GA_3$. On TDZ, zeatin, spermine and spermidine, expectant results were not obtained except a little affirmative effect of spermidine ($69{\pm}2.88$ at 1 mg/L).

Evaluation of Forest Watershed Hydro-Ecology using Measured Data and RHESSys Model -For the Seolmacheon Catchment- (관측자료와 RHESSys 모형을 이용한 산림유역의 생태수문 적용성 평가 -설마천유역을 대상으로-)

  • Shin, Hyung Jin;Park, Min Ji;Kim, Seong Joon
    • Journal of Korea Water Resources Association
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    • v.45 no.12
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    • pp.1293-1307
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    • 2012
  • This study is to evaluate the RHESSys (Regional Hydro-Ecological Simulation System) simulated streamflow (Q), evapotranspiration (ET), soil moisture (SM), gross primary productivity (GPP) and photosynthetic productivity (PSNnet) with the measured data. The RHESSys is a hydro-ecological model designed to simulate integrated water, carbon, and nutrient cycling and transport over spatially variable terrain. A 8.5 $km^2$ Seolma-cheon catchment located in the northwest of South Korea was adopted. The catchment covers 90.0% forest and the dominant soil is sandy loam. The model was calibrated with 2 years (2007-2008) daily Q at the watershed outlet and MODIS (Moderate Resolution Imaging Spectroradiometer) GPP, PSNnet and 3 year (2007~2009) daily ET data measured at flux tower using the eddy-covariance technique. The coefficient of determination ($R^2$) and the Nash-Sutcliffe model efficiency (ME) for Q were 0.74 and 0.63, and the average $R^2$ for ET and GPP were 0.54 and 0.93 respectively. The model was validated with 1 year (2009) Q and GPP. The $R^2$ and the ME for Q were 0.92 and 0.84, the $R^2$ for GPP were 0.93.

2형 당뇨병 치료제의 최근동향

  • 허갑범
    • Proceedings of the Korean Society of Applied Pharmacology
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    • 2001.04a
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    • pp.115-126
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    • 2001
  • 1988년 Reaven이 인슐린저항성증후군(insulin resistance syndrome)의 개념을 처음 소개한 후 전세계적으로 관심이 증가하고 있다. 인슐린저항성증후군은 대사성증후군 (metabolic syndrome), X 증후군(syndrome X)이라고도 하며, 인슐린 저항성에 따른 고인슐린혈증, 내당능장애(impaired glucose tolerance)와 2형 당뇨병(인슐린 비의존형 당뇨병), 이상지질혈증(dyslipidemia), 고혈압 등 일련의 질환은 동일인에서 흔히 병발하고, 이들은 모두 죽상경화증(atherosclerosis)의 위험인자라는 관점에서 상호연관성을 갖는 질환군이라는 개념이다. 2형 당뇨병은 인슐린에 대한 말초조직의 저항성과 상대적으로 감소된 인슐린 분비능의 결과라고 할 수 있다. 또한 상당수의 2형 당뇨병은 비만, 특히 복부비만을 동반하고 있으며, 비만은 그 자체로 인슐린 저항성을 유발한다. 결국 인슐린 저항성이 장기간 개선되지 못하게 되면 2형 당뇨병이 유발되게 된다. 따라서 2형 당뇨병에 대한 최선의 치료는 혈당조절 뿐만 아니라 인슐린 저항성과 관련된 심혈관계 위험인자를 개선시키는 것을 목표로 해야 한다. 그 증거로 당뇨병 조절과 관련한 대규모 전향적 연구인 UKPDS(United Kingdom Prospective Diabetes Study)의 결과를 보면, 2형 당뇨병에서 설폰요소제(sulfonylurea)나 인슐린으로 10년동안 혈당조절을 했을 때 미세혈관 합병증은 의미있게 감소시켰으나 대혈관 합병증의 발생률은 의미있는 감소를 보이지 못하였다. 반면 대혈관 합병증이 미세혈관 합병증보다 사망에 기여하는 비율이 70배 이상이라는 사실은 당뇨병의 치료가 혈당조절에만 초점이 맞추어져서는 안 되며 심혈관계 위험인자를 개선시키는 치료가 동시에 진행되어야 한다는 점을 시사한다.시료에서 매우 드물게 관찰된다. 음극선발광(cathodoluminescence) 영상의 해석을 통해 저어콘 결정의 성장사를 유추하였으며, 이를 바탕으로 이온현미분석 점(spot)을 정하였다. U-Pb-Th 자료는 퍼스(Perth) 저어콘 스탠다드 (CZ3, 564 Ma, $^{206}$Pb/$^{238}$U=0.0914)를 사용하였다. 아래에 기술하는 연대는 모두 $^{206}$Pb/$^{238}$U 연대에 해당된다. 두 개의 화강암질 편마암 시료로부터 구한 U-Pb 저어콘 연대는 각각 812 $\pm$ 14 Ma(1006-8)와 822 $\pm$ 17 Ma(1006-9)로 분석오차 내에서 서로 일치한다. 이 결과는 춘천 및 전곡 지역의 석류석 각섬암에서 보고된 Sm-Nd 전암연대(852 $\pm$ 24 Ma 및 824 $\pm$ 143 Ma; Lee and Cho, 1995; Ree et al., 1996)와 잘 부합한다. 따라서 후기 원생대 기간 중 화성활동이 한반도에서 광범위하게 일어났음을 시사한다. 한편, 1006-9 시료에서는 예외적으로 한 개의 저어콘 입자 주변부(rim)에서 매우 얇은 과성장띠가 관찰되었으며, 두 개의 점 분석으로부터 구한 U-Pb 저어콘 연대는 약 235 Ma이다. 이 띠는 또한 변성기원의 저어콘에서 흔히 관찰되는 작은 W (<0.05) 비를 보인다. 1006-5 시료는 위 두 시료로부터 수 km 떨어진 지점에서 채집하였으나, 저어콘 연대는 상이한 기록을 보여준다. 즉 매우 작은 Th/U (<0.01) 값을 갖는 저어콘의 주변부에서 223 $\pm$ 5 Ma의 연대가 잘 정의되며, 이는 1006-9 시료에서 관찰된 결과와

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Therapeutic Antiallergy Effect of Fermented Soy Curd by Pediococcus inopinatus Y2 (항알레르기에 대한 Pediococcus inopinatus Y2 유산균 발효 두유 커드의 치료 효과)

  • Kang, Moon-Sun;Lee, Young-Geun;Kim, Han-Soo;Chung, Hun-Sik;Hwang, Dae-Youn;Kim, Dong-Seob
    • Journal of Life Science
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    • v.29 no.4
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    • pp.478-483
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    • 2019
  • The use of fermented soy curd as a functional substance has been actively studied due to the anti-inflammatory and antiallergic activity of soybean protein hydrolyzate by enzymes of lactic acid bacteria. The present study investigated the potential of soy curd as a treatment for atopic dermatitis (AD). Pediococcus inopinatus Y2 (P. inopinatus Y2) lactic acid bacteria were inoculated into soy milk and fermented ($30^{\circ}C$, 24 hr). Changes in body weights, ear thicknesses, IgE concentrations, and weights of immune organs in ICR female mice were quantified. Moderate weight gain occurred in most of the groups. The ear thickness was lowest in the untreated group (no group), and it was allergic and thickened in the phthalic anhydride (PA) treatment group. Based on visual observations, as compared with the skin condition of the PA-induced AD group, the skin condition of the animals in the fermented soy curd (FSC) group was improved. Therefore, FSC by lactic acid bacteria seemed to improve AD. Based on the comparison of the weights of the spleen, thymus, and lymph nodes, as well as the results of the IgE analysis, soy milk, in addition to FSC, had a therapeutic effect. However, the antiallergy effects of FSC in terms of AD were far superior to those of soy milk. These results indicated that FSC can be used as a treatment for AD.

Yield characteristics of Pleurotus ostreatus according to the use of spent mushroom substrate with high nitrogen content (질소원이 증진된 수확후배지를 이용한 느타리버섯 수량 특성)

  • Baek, Il-Sun;Kim, Jeong-Han;Lee, Yong-Seon;Shin, Bok-Eum;Lee, Yun-Hae;Lee, Young-Soon
    • Journal of Mushroom
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    • v.16 no.4
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    • pp.257-262
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    • 2018
  • The aim of this study was to re-use spent mushroom substrate (SMS) with increased total nitrogen (T-N) and amino acid content and reduce the amount of cottonseed meal used as nutrient supplement in Pleurotus ostreatus cultivation. Bacteria used for improvement of the T-N content were GM20-4(Bacillus sp.) and Rhodobacter sphaeroides (RS). GM20-4 was isolated from the SMS of P. ostreatus and RS was obtained from Gwangjusi agricultural technology center. SMS in T1, T2, and T3 was reused as substrate after drying and the T-N content of dried SMS (D-SMS) was increased by 0.34% by treatment with the bacteria. T1 with 8% D-SMS and T2 with 18% D-SMS had higher rates of primordia formation compared with T3 and the control. The biological efficiency of the control and of treatment with 8%, 18%, and 26% D-SMS was 110%, 114%, 112%, and 79%, respectively. Considering the economic cost, yield, and biological efficiency, T2 with 18% D-SMS as the culture substrate for P. ostreatus was shown to be the most effective for cultivation.

Protective effect of Artemisiae Capillaris Herba water extract on liver injury induced by thioacetamide (인진호 열수 추출물이 thioacetamide에 의해 유발된 간손상에 미치는 간보호 효과)

  • Kim, Min Ju;Lee, Jin A;Shin, Mi-Rae;Park, Hae-Jin;Roh, Seong-Soo
    • Journal of Nutrition and Health
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    • v.54 no.4
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    • pp.412-421
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    • 2021
  • Purpose: Thioacetamide (TAA) produces reactive oxygen species (ROS) in the liver, and the generated ROS induces liver injury through inflammatory reactions. The current study was undertaken to investigate the hepatoprotective effect of Artemisiae Capillaris Herba water extract (AC), imparted via its antioxidant activity, in an animal model of TAA-induced liver injury. Methods: Animal experiments were conducted in 5 groups: normal, control (TAA 200 mg/kg), SM (TAA 200 mg/kg + silymarin 100 mg/kg), ACL (TAA 200 mg/kg + AC 100 mg/kg), ACH (TAA 200 mg/kg + AC 200mg/kg). TAA (intraperitoneal) and treatment compounds (per oral) were administered for 3 days. Serum levels of ammonia concentration and myeloperoxidase (MPO) activity were subsequently measured. Liver tissues were subjected to western blot analysis for measuring the oxidative stress (NADPH oxidase), anti-oxidative activity (Nrf2, heme oxygenase-1 [HO-1], superoxide dismutase [SOD], catalase, and GPx-1/2), tissue inhibitors of metalloproteinase (TIMP)-1, and matrix metalloproteinases (MMPs) protein expressions. Results: Serum ammonia levels and MPO activity were significantly increased in the TAA-induced control group, whereas groups administered AC treatment showed markedly reduced levels. Western blot analysis revealed significantly increased NOX2 and p22phox expressions, (oxidative stress-related factors) in the TAA-induced control group. These levels were determined to be significantly decreased after AC exposure. Moreover, antioxidant-related factors including Nrf2, HO-1, SOD, catalase, and GPx-1/2 were significantly decreased in the control group and increased in the AC treated groups. In addition, MMP expressions were significantly suppressed in the AC treatment group due to increased levels of TIMP-1. Conclusion: Taken together, these data indicate that exposure to AC reduces the oxidative stress by inhibiting the expression of NADPH oxidase (NOX2 and p22phox) through the Nrf2 signaling pathway. We therefore propose the potential of AC for the prevention and treatment of TAA-induced liver injury.