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Isolation, Characterization and Numerical Taxonomy of Novel Oxalate-oxidizing Bacteria

  • Sahin, Nurettin;Gokler, Isa;Tamer, Abdurrahman
    • Journal of Microbiology
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    • v.40 no.2
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    • pp.109-118
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    • 2002
  • The present work is aimed at providing additional new pure cultures of oxalate utilizing bacteria and its preliminary characterization for further work in the field of oxalate-metabolism and taxonomic studies. The taxonomy of 14 mesophilic, aerobic oxalotrophic bacteria isolated by an enrichment culture technique from soils rhizosphers, and the juice of the petiole/stem tissue of plants was investigated. Isolates were characterized with 95 morphological, biochemical and physiological tests. Cellular lipid components and carotenoids of isolates were also studied as an aid to taxonomic characterization. All isolates were Gram-negative, oxidase and catalase positive and no growth factors were required. In addition to oxalates, some of the strains grow on methanol and/or formate. The taxonomic similarities among isolates, reference strains or previously reported oxalotrophic bacteria were analysed by using the Simple Matching (S/ sub SM/) and Jaccard (S$\_$J/) Coefficients. Clustering was performed by using the unweighted pair group method with arithmetic averages (UPGMA) algorithm. The oxalotrophic strains formed five major and two single-member clusters at the 70-86% similarity level. Based on the numerical taxonomy, isolates were separated into three phenotypic groups. Pink-pigmented strains belonged to Methylobacterium extorquens, yellow-pigmented strains were most similar to Pseudomonas sp. YOx and Xanthobacter autorophicus, and heterogeneous non-pigmented strains were closely related to genera Azospirillum, Ancylobacter, Burkholderia and Pseudomonas. New strains belonged to the genera Pseudomonas, Azospirillum and Ancylobacter that differ taxonomically from other known oxalate oxidizers were obtained. Numerical analysis indicated that some strains of the yellow-pigmented and nonpigmented clusters might represent new species.

Experimental Study on the Shear Behavior of Reinforced Hooked-Steel-Fiver Concrete Beams (훅트강섬유보강 철근콘크리트보의 전단거동에 대한 실험적 연구)

  • 심종성;이차돈;김규선;오홍섭
    • Magazine of the Korea Concrete Institute
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    • v.7 no.5
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    • pp.179-188
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    • 1995
  • SFRC overcomes brittleness of concrete and has increases strength due to the action of confmement, crack arrestmg mechan~sm and pull out resistances of steel f~bers ~ n s ~ d e the concrete. These lead also to the increased strength and ductility under the shear stress. It has been reported that the secondary remforcement effect of steel fibers IS more pronounced In shear than flexure. Addition of hooked stee!, fibers into the cementitious materials enhanced shear resistance and consequently improves structural behavior and shear strength of Reinforced Hooked-Steel-Fiber Concrete Ream(RHSFCI3) under the shear forces. Experimental observations were made on the main parameters effecting structural behavior of RHSFCB in this study. The volume fractions of fibers, shear span to depth ratios, and spaclngs of stlrrups were taken into account as the mam parameters. Some eyuatlons reported in the literatures, regardmg the predict~ons of the shear strength of RHSFCB have been evaluated stdtlst~cdlly based on the tot a1 number of 95 test results on RHSFCB faded In shear on shear flexu~al mode.

THE EFFECT OF HYBRID LAYER THICKNESS ON MICROTENSILE BOND STRENGTH OF THREE-STEP AND SELF-ETCHING DENTIN ADHESIVE SYSTEMS (혼성층의 두께가 three-step과 self-etching 상아질 접착제의 미세인장결합강도에 미치는 효과)

  • Lee, Hye-Jung;Park, Jeong-Kil;Hur, Bock
    • Restorative Dentistry and Endodontics
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    • v.28 no.6
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    • pp.491-497
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    • 2003
  • The purpose of this study was to evaluate the correlation between hybrid layer thickness and bond strength using confocal laser scanning microscope and microtensile bond strength test of two adhesive systems. The dentin surface of human molars. sectioned to remove the enamel from the occlusal surface. Either Scotchbond Multi-Purpose(3M Dental Product, St. Paul, MN, U.S.A) or Clearfil SE Bond (Kuraray, Osaka, Japan) was bonded to the surface. and covered with resin-composite. The resin-bonded teeth were serially sliced perpendicular to the adhesive interface to measure the hybrid layer thickness by confocal laser scanning microscope. The specimen were trimmed to give a bonded cross-sectional surface area of $1\textrm{mm}^2$, then the micro-tensile bone test was performed at a cross head speed of 1.0 mm/min. All fractured surfaces were also observed by stereomicroscope. There was no significant differences in bond strengths the materials(p>0.05). However. the hybrid layers of three-step dentin adhesive system, SM, had significantly thicker than self-etching adhesive system. CS(p<0.05). Pearson's correlation coefficient showed no correlation between hybrid layer thickness and bond strengths(p>0.05). Bond strengths of dentin adhesive systems were not dependent on the thickness of hybrid layer.

Genetic sequence and phylogenetic analysis of spike genes of Porcine epidemic diarrhea virus (PEDV) in Chung-Nam area (충남지역 돼지유행성설사 바이러스 Spike 유전자 염기서열 및 계통분석)

  • Park, Hyo-Seon;Yook, Sim-Yong;Jeon, Dong-Min;Lee, Jin-Ju;Shin, Chang-Ho
    • Korean Journal of Veterinary Service
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    • v.39 no.4
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    • pp.259-266
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    • 2016
  • Porcine epidemic diarrhea virus (PEDV) causes an acute and lethal watery diarrhea in piglets that is great economic losses to the swine country worldwide. The spike (S) glycoprotein is an important determinant for PEDV biological properties. In the present study, we determined the full-length S gene sequences of five Chung-nam PEDV field isolates collected in 2016. The S gene was amplified by RT-PCR, purificated, sequenced, analyzed and then compared with published sequences of other PEDV strains. 5 field strains share 98.5%~99.9% homologies with each other at the nucleotide sequence level and 96.7%~99.9% homologies with each other at the amino acids sequence level. Most field strains have nucleotide insertions, deletions and mutation regions, and show lower homologies (93.1~93.8%) with classical and vaccine strains, however higher homologies (99.1%~99.5%) with US PEDV isolates in 2013. By phylogenetic tree analysis based on nucleotide sequence, five PEDV field isolates were clustered into Genogroup 2b but differ genetically from the vaccine strains (SM-98 and DR-13).

Radiopharmaceuticals for the Therapy of Metastatic Bone Pain (뼈전이의 방사성동위원소 통증치료)

  • Ahn, Byeong-Cheol
    • Nuclear Medicine and Molecular Imaging
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    • v.40 no.2
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    • pp.82-89
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    • 2006
  • Bone metastasis is a common sequelae of solid malignant tumors such as prostate, breast, lung, and renal cancers, which can lead to various complications, including fractures, hypercalcemia, and bone pain, as well as reduced performance status and quality of life it occurs as a result of a complex pathophysiologic process between host and tumor cells leading to cellular invasion, migration adhesion, and stimulation of osteoclastic and osteoblastic activity. Several sequelae occur as a result of osseous metastases and resulting bone pain can lead to significant debilitation. A multidisciplinary approach is usually required not only to address the etiology of the pain and its complicating factors but also to treat the patient appropriately. Pharmaceutical therapy of bone pain, includes non-steroidal analgesics, opiates, steroids, hormones, bisphosphonates, and chemotherapy. While external beam radiation therapy remains the mainstay of pain palliation of a solitary lesions, bone seeking radiopharmaceuticals have entered the therapeutic armamentarium for the treatment of multiple painful osseous lesions. $^{32}P,\;^{89}SrCl,\;^{153}Sm-EDTMP,\;^{188}Re/^{186}Re-HEDP,\;and\;^{177}Lu-EDTMP$ can be used to treat painful osseous metastases. These various radiopharmaceuticals have shown good efficacy in relieving bone pain secondary to bone metastasis. This systemic form of metabolic radiotherapy is simple to administer and complements other treatment options. This has been associated with improved mobility in many patients, reduced dependence on narcotic and non-narcotic analgesics, improved performance status and quality of life, and, in some studios, improved survival. All of these agents, although comprising different physical and chemical characteristics, offer certain advantages in that they are simple to administer, are well tolerated by the patient if used appropriately, and can be used alone or in combination with the other forms of treatment. This article illustrates the salient features of these radiopharmaceuticals, including the usual therapuetic dose, method of administration, and indications for use and also describe about the pre-management checklists, and jndication/contraindication and follow-up protocol.

A Semiotic Approach to Modern Visual Arts (시각예술의 기호학 연구)

  • 남택운
    • The Journal of the Korea Contents Association
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    • v.3 no.2
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    • pp.1-10
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    • 2003
  • A semiotic approach to modem visual arts has emerged since French post-structuralism was introduced to Anglo -American academics by "deconstruction" or "postmodemi sm." It views a work of art as a sign, which is its math odical assumption and, at the same time, makes its appli cation more accessible. In the milieu of modem visual arts' effort to be intimate with general audience, modem art photography is now fared with the request to be a familar and universal domain, instead of being left only in photo books as artistic and academic achievements. More specifically, various photo images puter graphics to such megaexhibitions as "Gwangju Biennale,. "Media City Seo ul," and "Pusan International Art Festival," are main objects of study. A coherent and scientific analysis of visual semitotics is still on the way, however, it is an urgent task how to read and interpret a photo image with multiple meanings. This study argues that visual seniotics can be a powerful tool to enhance the understanding of art photography. After all, seniotics is a product of age; we live in the age of legibility, that is, of reading the work of art well as the social events and phenomena. art well as the social events and phenomena.

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Development of Cellulose Strip for Dry Eye Inspection (건성안검사용 셀룰로즈 스트립 개발)

  • Lee, Myeonggu;Jeong, Myeong-jin
    • The Journal of the Convergence on Culture Technology
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    • v.5 no.4
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    • pp.355-359
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    • 2019
  • Schirmer test is one of the most used methods for the diagnosis of dry eye. We attempted to develop a tear level measurement tool to replace unreliable Schirmer test with inaccurate results. Absorbency tests for various absorbents were carried out. As a result, ${\alpha}$ cellulose pulp was selected as the absorbent. Prototypes were produced and evaluated twice. Finally a tear level measurement tool in the form of a strip of ${\alpha}$ cellulose pulp adhered to a polyurethane was prepared. Usability evaluation of prepared tear level measurement tool was performed. As a result, it was confirmed that it has a significant correlation with SM tube developed oversea recently. In addition, it was judged to be useful as an alternative to the Schirmer test in terms of measurement time and accuracy.

Pregnancy diagnosis in goat by using vaginal cytology and trans-abdominal ultrasonography

  • Ali, Md. Aziz;Islam, Md. Faruk;Rahman, SM Latifur;Zohara, Begum Fatema
    • Journal of Animal Reproduction and Biotechnology
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    • v.35 no.4
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    • pp.338-346
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    • 2020
  • The present study was conducted in a goat farm for pregnancy diagnosis by using vaginal cytology and B-mode real time ultrasound using 5 MHz probe by transabdominal method. Seventeen pregnant does were used for this study. The objective of the study was to determine the earliest day of pregnancy and describe the chronological characteristics of pregnancy from day 22 to day 40 for vaginal cytology and day 25 to day 60 for ultrasonography of gestation. The differences among the average percentage of cell value in different age of pregnancy were significant (p < 0.05). The average percentage of intermediate cells (81.12%) was also significantly (p < 0.05) higher than superficial (9.41%), keratinized (7.10%) and neutrophil (2.61%) on 22-40th days of pregnancy. In case of real time B-mode ultrasonography, the gestational sac was observed only in three does out of seventeen (17.6 %) at 25-30 days whereas the placentomes and heart beat of the foetus were first detected at 31-35 days in six does (35.3%). The foetal leg buds were first visualized at 36-40 days in four does (23.5%) whereas the foetal vertebral column was first observed at 36-40 days of gestation in only three does (17.6%). In conclusion, vaginal cytology and trans-abdominal ultrasonography can be used for detection of early pregnancy in does.

Effects of age and diet forms on growth-development patterns, serum metabolism indicators, and parameters of body fat deposition in Cherry Valley ducks

  • Lv, Gang;Zeng, Qiufeng;Ding, Xuemei;Bai, Shiping;Zhang, Keying
    • Animal Bioscience
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    • v.35 no.2
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    • pp.247-259
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    • 2022
  • Objective: This study was conducted to investigate the effects of age and diet forms on growth-development patterns, serum metabolism indicators, and parameters of body fat deposition in Cherry Valley ducks. Methods: According to the hatching age and initial weight, a total of 150 1-day-old male SM3 Cherry Valley ducks were randomly assigned to two diet forms (pellet vs powder form). Each treatment had with 5 replicates per treatment and 15 meat ducks per replicate. The study lasted 42 d, which was divided into two periods (1 to 21 vs 22 to 42 d). Results: Our results showed that compared with powder group, ducks in pellet group had greater growth performance during different period (p<0.05). The inflection point was 24 d and was not numerically affected by diet forms. Increasing age (42 vs 21 d) significantly increased the weight of body fat and hepatic fat metabolism related enzyme activities in ducks (p<0.05), meanwhile, increasing age (42 vs 21 d) improved serum metabolism indicators and decreased mRNA expression levels of fat metabolism-related genes in liver (p<0.05). Ducks fed different diets (pellet vs powder form) increased growth performance as well as the weight of body fat and improved serum metabolism indicators (p<0.05). In addition, interactions were found between age and diet forms on the levels of serum metabolism indicators in ducks (p<0.05). Conclusion: In conclusion, powder feed reduced growth performance of ducks, and the day of inflection point was 24 days old. Ducks with higher age or fed with pellet diet showed higher fat deposition. The effect of age and feed forms on body fat deposition might result from changes in the contents of serum metabolism indicators, key enzyme activity of lipid production, and hepatic gene expressions.

Integration of top-down and bottom-up approaches for a complementary high spatial resolution satellite rainfall product in South Korea

  • Nguyen, Hoang Hai;Han, Byungjoo;Oh, Yeontaek;Jung, Woosung;Shin, Daeyun
    • Proceedings of the Korea Water Resources Association Conference
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    • 2022.05a
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    • pp.153-153
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    • 2022
  • Large-scale and accurate observations at fine spatial resolution through a means of remote sensing offer an effective tool for capturing rainfall variability over the traditional rain gauges and weather radars. Although satellite rainfall products (SRPs) derived using two major estimation approaches were evaluated worldwide, their practical applications suffered from limitations. In particular, the traditional top-down SRPs (e.g., IMERG), which are based on direct estimation of rain rate from microwave satellite observations, are mainly restricted with their coarse spatial resolution, while applications of the bottom-up approach, which allows backward estimation of rainfall from soil moisture signals, to novel high spatial resolution soil moisture satellite sensors over South Korea are not introduced. Thus, this study aims to evaluate the performances of a state-of-the-art bottom-up SRP (the self-calibrated SM2RAIN model) applied to the C-band SAR Sentinel-1, a statistically downscaled version of the conventional top-down IMERG SRP, and their integration for a targeted high spatial resolution of 0.01° (~ 1-km) over central South Korea, where the differences in climate zones (coastal region vs. mainland region) and vegetation covers (croplands vs. mixed forests) are highlighted. The results indicated that each single SRP can provide plus points in distinct climatic and vegetated conditions, while their drawbacks have existed. Superior performance was obtained by merging these individual SRPs, providing preliminary results on a complementary high spatial resolution SRP over central South Korea. This study results shed light on the further development of integration framework and a complementary high spatial resolution rainfall product from multi-satellite sensors as well as multi-observing systems (integrated gauge-radar-satellite) extending for entire South Korea, toward the demands for urban hydrology and microscale agriculture.

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