• Journal of Microbiology and Biotechnology
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• v.23 no.4
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• pp.545-554
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• 2013

Plasmid isolation of kimchi-derived Weissella cibaria KLC140 revealed six different plasmids. The smallest plasmid, pKW2124, was DNA sequenced and characterized, showing 2,126 bp with a GC content of 36.39% and five putative open reading frames (ORFs). In silico analysis of these ORFs showed ORF1 encodes a putative replication protein similar to rolling circular replication proteins from other lactic acid bacteria. However, a single-stranded intermediate was not detected when S1 nuclease was treated, suggesting it may follow theta replication. Interestingly, the replication initiation site of this plasmid is 100% identical to other plasmids from lactic acid bacteria, suggesting it may function for replication initiation. To construct a surface layer expression vector, pTSLGFP, slpA encoding the surface layer protein from Lactobacillus acidophilus was PCR amplified and fused with the gfp gene, forming a SLGFP fused gene. The plasmid pKW2124 was cloned into the XbaI site of pUC19, forming an Weissella-E. coli shuttle vector pKUW22. NheI-linearized pTSLGFP was ligated into pKUWCAT containing pKUW22 and the chloramphenicol acetyltransferase gene from pEK104, resulting in an 8.6 kb pKWCSLGFP surface layer expression vector. After transformation of this vector into W. cibaria KLC140, a GFP fluorescence signal was detected on the surface of the transformant, substantiating production of SLGFP fused protein and its secretion. This is the first report for construction of a Weissella surface layer expression vector, which may be useful for surface layer production of beneficial proteins in Weissella.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.32 no.2
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• pp.149-154
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• 1999

A 16-week feeding trial was conducted to determine the amount of fish meal analogue (FMA) that can be replacing white fish meal (WFM) protein in Korean rockfish. Five experimental diets were formulated on isonitrogenous and isocaloric basis of $50\%$ crude protein and 15.6 KJ/g diet. The graded level of replacement of PM by Em on the basis of crude protein were as follows : Diet 1($100\%$ WFM), Diet 2($87.5\%$ WFM : $12.5\%$ FMA), Diet 3 ($75\%$ WFM : $25\%$ FMA), Diet 4($75\%$ WFM : $25\%$ FMA+2 EAAs), Diet 5 ($50\%$ WFM : $50\%$ FMA+2 EAAs). Methionine and Iysine were selected for 2 essential amino acids (EAA) to study the effect of adding EAA to the diets 4 and 5. Weight gain (WG), feed fed efficiency (FE) and protein efficiency ratio (PER) of fish fed diet 3, 4 and 5 were significantly lower (P< 0.05) than those of fish fed the control ($100\%$ WFM),while that of fish fed diet 2 was not significantly different diet(P>0.05) from those of fish fed the control diet. WG, FE and PER of fish fed diet 4 were significantly higher (P<0.05) than those of fish fed diet 3, but lower than those of fish fed the control diet. Therefore, these results indicated that FMA protein could substitute WFM protein up to $12.5\%$ without 2 EAAs supplementation in Korean rockfish. And, dietary supplementation of 2 EAAs could be benetficial when FMA is utilized as a fish meal replacer in juvenile Korean rockfish.

• Journal of the Ergonomics Society of Korea
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• v.33 no.1
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• pp.39-48
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• 2014

Objective: This entire study has two parts. Study I aimed to develop a psychological assessment scale and the study II aimed to investigate the effects of LFN (low frequency noise) on the psychological responses in humans, using the scale developed in the study I. Background: LFN is known to have a negative impact on the functioning of humans. The negative impact of LFN can be categorized into two major areas of functioning of humans, physiological and psychological areas of functioning. The physiological impact can cause abnormalities in threshold, balancing and/or vestibular system, cardiovascular system and, hormone changes. Psychological functioning includes cognition, communication, mental health, and annoyance. Method: 182 college students participated in the study I in development of a psychological assessment scale and 42 paid volunteers participated in the study II to measure psychological responses. The LFN stimuli consisted of 12 different pure tones and 12 different 1 octave-band white noises and each stimulus had 4 different frequencies and 3 different sounds pressure levels. Results: We developed the psychological assessment scale consisting of 17 items with 3 dimensions of psychological responses (i.e., perceived physical, perceived physiological, and emotional responses). The main findings of LFN on the responses were as follows: 1. Perceived psychological responses showed a linear relation with SPL (sound pressure level), that is the higher the SPL is, the higher the negative psychological responses were. 2. Psychological responses showed quadric relations with SPL in general. 3. More negative responses at 31.5Hz LFN than those of 63 and 125Hz were reported, which is deemed to be caused by perceived vibration by 31.5Hz. 'Perceived vibration' at 31.5Hz than those of other frequencies of LFN is deemed to have amplified the negative psychological response. Consequently there found different effects of low frequency noise with different frequencies and intensity (SPL) on multiple psychological responses. Conclusion: Three dimensions of psychological responses drawn in regard to this study differed from others in the frequencies and SLP of LFN. Negative psychological responses are deemed to be differently affected by the frequency, SPL of the LFN and 'feel vibration' induced by the LFN. Application: The psychological scale from our study can be applied in quantitative psychological measurement of LFN at home or industrial environment. In addition, it can also help design systems to block LFN to provide optimal conditions if used the study outcome, .i.e., the relations between physical and psychological responses of LFN.

• Proceedings of the Korea Water Resources Association Conference
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• 2012.05a
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• pp.118-118
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• 2012

수자원의 효율적 이용 및 관리는 심화되고 있는 기후변화에 선제적으로 대응하고 발생가능한 물위기에 대비하기 위한 필수조건이다. 그 중 가장 핵심이 되는 요소는 댐에 저수된 물을 효과적으로 이용하는 것, 즉 댐 건설목적에 따라 시간 및 공간별로 적절하게 할당시키는 것이라고 할 수 있다(Kim, 1998). 그러나 단일 댐의 운영과는 달리 수계내 댐군의 연계 운영은 매우 복잡하고 어려운 문제이다. 연계된 댐들간 저수 상황을 고려하여 유역내 시 공간적인 용수 수요의 지속적인 충족을 위하여 유입량 예측의 정확성을 높이도록 하고, 상류 댐에 최대한 저류하도록 하며, 여수로 방류 같은 불필요한 방류를 최소화 하고, 서로 상충되는 목표를 갖고 있지만, 홍수용량 및 발전수위를 최대로 확보하도록 하여야 한다. 이처럼 댐 운영을 위한 실제 상황은 단일 목적에 의한 최적화와는 달리 여러 상충되는 목적 및 구성 요소들간의 타협, 조정을 필요로 한다. 댐군의 연계운영 문제는 1960년대 초부터 현재까지 활발히 연구가 진행되어 온 분야 중 하나이나 문제의 복잡성과 어려움으로 인해 아직까지도 최선의 방안을 제시하기 어려운 문제이다(ReVelle, 2000). 이를 위한 방법은 시뮬레이션 모형 활용기법과 최적화 모형 활용기법으로 대별할 수 있으며 각 방법의 서로 다른 구조적 특성과 장단점으로 인하여 이원화된 체계로 사용되는 것이 현재의 국내 실정이다. 대부분의 실무에서는 이해도도 쉽고, 비교적 결과를 빨리 도출할 수 있는 시뮬레이션 기반의 모형을 활용하며 대표적으로 HEC-5, K-ModSim, HEC-ResSim 등이 활용되어왔다. 반면, 학계에서는 DP, MIP, SLP, SDP 등 최적화기법을 댐운영에 활용 할 것을 제안하고 있지만, 활용에 대한 거부감이 남아있는 것이 현실이다. 본 연구에서는 시뮬레이션과 최적화기법의 원론적 비교를 통해 각 방법의 장단점과 한계점을 분석하고, 왜 이원화된 사용체계로 되었는지에 대한 고찰과 이에 대한 해결책으로 시뮬레이션모형의 장점과 최적화기법의 장점을 결합한 모형을 제안한다. 국내에는 Kim and Park(1998)이 시뮬레이션 기반의 최적화 모형 CoMOM(Coordinated Multi-Reservoir Operating Model)을 개발하였으며, 이후 21C프론티어 연구사업(2001-2011)에서 모형의 보완수정 검증을 통해 실무 활용도를 높여 왔다. 본 연구를 통하여 거부감의 원천을 추적해 보고, 타당한 이유가 있는지 대한 것을 심층 분석해보고, CoMOM모형과 시뮬레이션 모형, 다른 최적화 기법들과의 원론적 비교를 통해 각 방법들의 효율적인 활용방안과 최적화모형의 구체적인 활용방안을 제시하고자 한다.

• 재활복지
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• v.22 no.4
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• pp.103-121
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• 2018

For knowing the effects of aging on conversational skills in daily communication, this paper studied for the conversational turn-taking skills, working memory and naming ability on healthy elderly adults over 65 ages. 85 elderly adults participated in this study, which divided into four groups by ages. Speech samples were collected in natural conversation. Memorization of numbers, mental calculation, repetition of words were administered for working memory test. K-BNT was used for the naming ability. One-way ANOVA analysis was used for the comparison of conversational turn-taking skills among four groups. We analyzed the correlation between conversational skills, working memory and naming ability. The results were as follows: first, there were a significant difference in conversational turn-taking skills by age, but not by gender. There was a significant difference in 'Turn-Taking Frequency' and 'Total Utterance Frequency' among four groups. The same results were shown in the scores of females within three groups(exclude groups over 85D)(p<.01). Second, there was a significant correlation between 'rates of maintenance' and 'naming ability'. In addition, it was found that the naming test predicted 'rates of maintenance' skills. The results of this study suggest that word-retrieval ability will be helpful to enhance functional communication skills in aging old adults.

• The Journal of the Korea Contents Association
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• v.19 no.4
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• pp.207-217
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• 2019

The current study aimed to examine how the caregivers and speech language pathologists (SLPs) would perceive the important communication contexts for 3~6 year-old children in the script-based intervention. Analytic Hierarchy Process analyses were conducted for the obtained survey data from SLPs and caregivers. Results showed that caregivers perceived 'educational and social life' and SLPs perceived 'family life' as the most important contexts from among three high-level contexts. Within each high-level context, 'meal time' in 'family life', 'playing with friends' in 'educational and social life', and 'playground' in 'culture and leisure life' were the most important communication contexts. Also, from among 35 global priority rank, caregivers perceived 'playing with friends' while SLPs perceived 'meal time' as the most important contexts, and 'family life' was high priority among first five ranked context both in caregivers and SLPs. In caregiver groups by children's age, 3 year- and 6 year-caregiver groups perceived 'family life' and 4 year- and 5 year-caregiver groups perceived 'educational and social life' as the most important contexts. There were also slight differences between caregiver groups by age in the rank order of communication contexts across the high level contexts. These results suggest that SLPs may need to consider not only child's age and developmental level but also caregiver's demands in selecting the appropriate communication contexts when delivering the script-based speech language intervention.

• Journal of Navigation and Port Research
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• v.44 no.6
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• pp.509-516
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• 2020

Korea has gradually been developing port distriparks in major domestic trade ports to diversify their function and create added-value. New tenant companies are needed to help achieve these goals, but no research has been done on selection criteria. To provide such criteria, this study conducted a comparative analysis of the productivity of tenant companies in Busan New Port Distripark based on their characteristics. SFP (single-factor productivity) was measured using the operational data of 67 companies in the distripark over the past - three years (2017-2019). The results indicate that the logistics business and the manufacturing business have strengths in cargo volume productivity and in sales productivity respectively. Also, Northern distripark, a relatively older facility, was found to be more productive than Ung-dong distripark. Finally, the effect of investment-both foreign and in FAC/EQ (facility and equipment)-on productivity showed an inverse relationship with productivity, with the companies with under-average investments being more productive than those whose investments were above average. Therefore, to enhance the productivity and competitiveness of port distriparks, tenant companies must be subject to supplemented system and law for increasing employment and cargo volume, and reestablished selection criteria.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.4 no.1
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• pp.11-26
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• 1984

The aim of this study is to establish a synthetical optimal method that simultaneously analyze and design reinforced concrete flat slab-column structures involving multi-constraints and multi-design variables. The variables adopted in this mathematical models consist of design variables including sectional sizes and steel areas of frames, and analysis variable of the ratio of bending moment redistribution. The cost function is taken as the objective function in the formulation of optimal problems. A number of constraint equations, involving the ultimate limit state and the serviceability limit state, is derived in accordance with BSI CP110 requirements on the basis of limit state design theory. Both objective function and constraint equations derived from design variables and an analysis variable generally become high degree nonlinear problems. Using SLP as an analytical method of nonlinear optimal problems, an optimal algorithm is developed so as to analyze and design the structures considered in this study. The developed algorithm is directly applied to a few reinforced concrete flat slab-column structures to assure the validity of it and the possibility of optimization From the research it is found that the algorithm developed in this study is applicable to the optimization of reinforced concrete flat slab column structures and it converges to a optimal solution with 4 to 6 iterations regardless of initial variables. The result shows that an economical design can be possible when compared with conventional designs. It is also found that considering the ratio of bending moment redistribution as a variable is reasonable. It has a great effect on the composition of optimal sections and the economy of structures.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.31 no.3
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• pp.380-385
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• 1998

This study evaluated the possible utilization and the replacing range of animal by-product mixture (ABPM) as a dietary fish meal replacer in growing common carp (Cyprinus carpio). ABPM is a mixture of leather meal, meat and bone meal, blood meal and squid liver powder at a specific weight based ratio. Five different diets were formulated on isonitrogenous and isocaloric basis of $40\%$ crude protein and 15.3 KJ/g diet in which white fish meal (WFM) protein was substituted with the ABPM Protein as follows: diet 1, $100\%$ WFM ($0\%$ ABPM, control); diet 2, $75\%$ WFM+$25\%$ ABPM ($25\%$ ABPM); diet $3,\;50\%$ WFM+$50\%$ ABPM ($50\%$ ABPM); diet 4 $25\%$ WFM+$75\%$ ABPM ($75\%$ ABPM); diet $5.0\%$ WFM+$100\%$ ABPM ($100\%$ ABPM). As the dietary protein sources, each diet contained $34.7\%$ of animal protein supplied by white fish meal and/or ABPM and $65.3\%$ of plant protein. After one week of conditioning period, fish averaging 10 g were randomly assigned to each diet treatment as triplicate groups and fed one of the experimental diets for 12 weeks. Weight gain of fish fed diet 1 (control) and 3 were significantly higher than those of fish fed diet 2, 4 and 5 during the first 4 weeks (P< 0.05), while there were no significant differences among all diet groups during the third 4 weeks (P>0.05). Feed conversion ratio of fish fed diet 1 was significantly higher than those fed diet 2, 3, 4 and 5 during the second 4 weeks (P<0.05), while there were no significant differences among all diet groups during the first and the third 4 weeks (P<0.05). There were no significant differences in proximate analysis among fish fed the experimental diets either for the second 4 weeks or the third 4 weeks (P>0.05). These results indicated that ABPM could be used as a fish meal replacer up to $100\%$ in growing common carp.