• 생명과학회지
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• 제28권11호
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• pp.1321-1331
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• 2018

본 연구에서 N. commune으로부터 U937과 SK-HEP-1 세포에 대해 apoptosis를 유도하는 광과민성물질을 순수 분리하였다. N. commune을 dichloromethane : Methanol (1:1)로 추출하여 Hexane, EtOAc, MeOH로 분획하였다. 높은 광역학활성을 나타내는 EtOAc 분획물에서 NC-1을 분리하였으며, 분자량 870의 pheophytin a ($C_{55}H_{74}N_4O_5$)인 것으로 구조를 동정하였다. 광역학활성 조사는 NC-1을 $1.15{\mu}M$에서 $23.00{\mu}M$까지 5개의 농도를 처리하여 광을 조사하는 것과 조사하지 않는 조건으로 나누어 평가하였다. U937과 SK-HEP-1 두 세포에서 apoptotic body 형성, cell blebbing 등의 형태적 변화와 세포독성, caspase 활성, 세포핵의 응축, DNA 단편화 등이 광 의존적이며 농도 의존적으로 활성이 증가하였다. 추가적으로 유세포분석을 통한 apoptosis 유도의 정량적 분석 결과에서도 광조건에서 농도가 증가할수록 apoptosis 유도가 증가하는 것을 확인하였다. 이러한 결과를 통해 NC-1의 U937과 SK-HEP-1 세포에 대한 사멸은 광역학적 apoptosis에 의한 것임을 확인하였다. 본 연구 내용을 N. commune으로부터 광감각제 개발의 기초자료로 활용할 수 있을 것이다.

• 한국경영정보학회:학술대회논문집
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• 한국경영정보학회 2007년도 추계학술대회
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• pp.309-315
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• 2007

SK텔레콤은 급변하는 이동통신시장 환경의 변화에 적절히 대응하기 위하여 지난 10여 년간 사용해 온 메인프레임 기반의 COIS 시스템을 중단하고 Unix 기반의 NGM 시스템으로 다운사이징하기로 결정하였다. 2002 년 9 월 SK 텔레콤은 차세대 IT 인프라 혁신 전략 프로젝트를 완료하고 이에 근거하여 2003 년 말 1 단계 프로젝트를 추진하게 된다. 그러나 개발 툴의 문제점, Governance 의 확보 실패 등 문제점이 계속 누적되어 2005년에 접어들어서는 총체적 난국 상황에 봉착하게 되었다. 2005 년 2 월 자체 점검 결과 개발 진척도는 50%에 불과한 상황이었다. 결국 2005 년 3 월, NGM 추진본부는 프로젝트의 추진 중단을 선언하기에 이르렀다. NGM 프로젝트는 처음부터 재 검토하여 Re-planning 을 실시한 후 구축을 재개하기로 결정하였다. 2005 년 5 월 NGM 프로젝트의 Re-plan 이 수립되었다. 우선 Turn-key 방식의 문제점을 해소하기 위하여 SK 텔레콤이 구축의 총책임을 떠맡고, SK C&C 가 시스템 이행에 대한 책임을 지는 Governance 체제를 확립하였다. 또한 2 단계 프로젝트에서는 NGM 프로젝트를 기술적으로 원점부터 재 검토하여 이전과는 근본적으로 다른 In-House 구축 접근방식을 채택하기로 하였다. 2 단계 프로젝트에서 관심을 집중한 기술적 의사결정 영역은 크게 (1) 미들웨어 및 개발프레임워크의 적용, (2) DB 아키텍처의 결정 및 슬림화, (3) 시스템 성능 개선 등의 세 분야로 집약할 수 있다. 이 논문에서는 이들 각각의 분야를 구체적으로 검토하였다. 결국 2006 년 10 월 9 일 메인프레임 기반의 COIS 는 Shut-down 되어 가동을 멈추었고, Unix 기반의 NGM 시스템이 가동을 개시함으로써 차세대 마케팅을 본격 추진하기 위한 다운사이징 프로젝트가 성공적으로 완수되었다.

• Journal of Microbiology and Biotechnology
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• 제14권5호
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• pp.914-918
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• 2004

This study was carried out to analyze the metabolic flux of W. kimchii sk10 on pyruvate and ethanol as a carbon source. The sk10 grown on ethanol produced acetate under aerobic conditions rather than under anaerobic conditions. The lactate and acetate were produced on ethanol plus pyruvate by the sk10 grown under aerobic and anaerobic conditions, respectively. The resting cell of sk10 produced 99.1 mM acetate and 17.3 mM lactate under aerobic conditions and 51.1 mM acetate and 62.4 mM lactate under anaerobic conditions from ethanol plus pyruvate, respectively. This result is thought to be due to the difference in the $NADH/NAD^+$ ratio depending on the growth conditions. The 11-fold overproduction of NADH peroxidase results in a low $NADH/NAD^+$ratio under aerobic growth conditions. At the low $NADH/NAD^+$ ratio, the metabolic flux of pyruvate toward lactate has to be shifted to a flux toward acetate without NADH oxidation to $NAD^+$, and ethanol oxidation to acetate coupled to $NAD^+$ reduction to NADH has to be activated.

• 한국산학기술학회:학술대회논문집
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• 한국산학기술학회 2004년도 춘계학술대회
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• pp.315-318
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• 2004

개비자 나무 추출물을 2종의 유방암 세포주(SOC-1395와 SK-BR-3)를 이용하여 MTT활성 분석을 수행하였다. RPMII-1640 배지에 $10\%$ FBS과 $1\%$의 penicillin-streptomycin 혼합용액을 사용하였으며, MTT을 농도로 PBS에 녹여 사용하였다. SK-BR-3 세포주가 개비자나무 추출물과 HHT(homoharringtonine)에 대하여 SCC-1395에 비하여 민감성이 높은 것으로 나타났다. 개비자나무의 추출시 응집제로 처리하고 건조 후 에탄을 추출물은 건조 후 에탄올 추출물에 비하여 SCC-1395 세포주가 높은 황성을 보였으며 SK-BR-3를 이용한 항암분석에서도 동일한 결과를 얻을 수 있었다.

• 유통과학연구
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• 제19권3호
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• pp.25-34
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• 2021

Purpose: The purpose of this study is to analyze the economic ripple effect(ERE) of logistics industry by construction of Trans-Korea Railway (TKR) and present policy measures to minimize the economic loss of South Korea (SK). Research design, data and methodology: As the analysis method, exponential smoothing was used for demand forecasting, Input-Output analysis was used to estimate the economic ripple effect coefficient, and scenario analysis was used to an efficient way to invest in TKR to minimize SK's economic losses. Results: 1) the production(logistics fares) of TKR for 10 years after its completion is about 11.42 trillion won in positive relations, and 26.89 billion won in negative relations. 2) the ERE of SK in positive relations is 24.32 trillion won in production inducement effect, 8.1 trillion won in value-added inducement effect, 3.54 trillion won in import inducement effect, and 70,930 persons in employment inducement effect. But the ERE was insufficient in the negative relations. 3) SK's efficient investment method is providing materials and equipment by SK and building the TKR by North Korea in positive inter-Korea relations. Conclusions: For the successful operation of TKR, international cooperation, legalization and stable peace settlement on the Korean Peninsula are required.

• 정보처리학회지
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• 제18권1호
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• pp.84-90
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• 2011

• Biomolecules & Therapeutics
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• 제22권3호
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• pp.207-212
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• 2014

Skin hyperpigmentation is one of the most common skin disorders caused by abnormal melanogenesis. The mechanism and key factors at play are not fully understood. Previous reports have indicated that cystamine (CTM) inhibits melanin synthesis, though its molecular mechanism in melanogenesis remains unclear. In the present study, we investigated the effect of CTM on melanin production using ELISA reader and the expression of proteins involved in melanogenesis by Western blotting, and examined the involvement of transglutaminase-2 (Tgase-2) in SK-MEL-2 human melanoma cells by gene silencing. In the results, CTM dose-dependently suppressed melanin production and dendrite extension in a-MSH-induced melanogenesis of SK-MEL-2 human melanoma cells. CTM also suppressed a-MSH-induced chemotactic migration as well as the expressions of melanogenesis factors TRP-1, TRP-2 and MITF in a-MSH-treated SK-MEL-2 cells. Meanwhile, gene silencing of Tgase-2 suppressed dendrite extension and the expressions of TRP-1 and TRP-2 in a-MSH-treated SK-MEL-2 cells. Overall, these findings suggested that CTM suppresses a-MSH-induced melanogenesis via Tgase-2 inhibition and that therefore, Tgase-2 might be a new target in hyperpigmentation disorder therapy.

• The Korean Journal of Physiology and Pharmacology
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• 제13권2호
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• pp.71-78
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• 2009

(S)-Carbamic acid 2-[4-(4-fluoro-benzoyl)-piperidin-1-yl]-1-phenyl-ethyl ester hydrochloride (YKP1447) is a novel "atypical" antipsychotic drug which selectively binds to serotonin (5-$HT_{2A}$, Ki=0.61 nM, 5-$HT_{2C}$, Ki=20.7 nM) and dopamine ($D_2$, Ki=45.9 nM, $D_3$, Ki=42.1 nM) receptors with over $10\sim100$-fold selectivity over the various receptors which exist in the brain. In the behavioral studies using mice, YKP1447 antagonized the apomorphine-induced cage climbing ($ED_{50}$=0.93 mg/kg) and DOI-induced head twitch ($ED_{50}$=0.18 mg/kg) behavior. In the dextroamphetamine-induced hyperactivity and conditioned avoidance response (CAR) paradigm in rats, YKP1447 inhibited the hyperactivity induced by amphetamine ($ED_{50}$=0.54 mg/kg) and the avoidance response ($ED_{50}$=0.48 mg/kg); however, unlike other antipsychotic drugs, catalepsy was observed only at much higher dose ($ED_{50}$=68.6 mg/kg). Based on the CAR and catalepsy results, the therapeutic index (TI) value for YKP1447 is over 100 (i.p.). These results indicate that YKP1447 has an atypical profile and less undesirable side effects than currently available drugs.

• Nutrition Research and Practice
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• 제11권4호
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• pp.307-318
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• 2017

BACKGROUND/OBJECTIVES: Relocation to new environments can have a negative impact on health by altering body weight and dietary patterns. This study attempted to elucidate changes in body weight, food security, and their current food and nutrient consumption in adult North Korean refugees (NKR) living in South Korea (SK). SUBJECTS/METHODS: This study analyzed data on 149 adult NKR from a North Korean refugee health in SK cohort at four time points (leaving North Korea, entering SK, first examination, and second examination). Body weight was self-reported at the two earlier time points and directly measured at the two later time points. Food security, diet-related behaviors (dietary habits and food consumption), and sociodemographic information were obtained using a self-administered questionnaire. Nutrient intake information was obtained by one-day 24-hour recall. Statistical analyses were performed with SPSS ver 23.0. RESULTS: Body weight increased during relocation by an average of 4 kg, although diversified patterns were observed during the settlement period in SK. Approximately 39.6% of subjects maintained their body weight between the first and second examinations, whereas 38.6% gained and 22.1% lost at least 3% of their body weight at the first examination by the second examination. Food security status improved from 12.1% food secure proportion to 61.7%. NKR showed generally good food and nutrient consumption (index of nutrient quality: 0.77-1.93). The body weight loss group showed the most irregular meal consumption pattern (P < 0.05), and eating-out was infrequent in all three groups. Consumption frequencies of food groups did not differ by group, except in the fish group (P = 0.036). CONCLUSION: This study observed considerable body weight adjustment during the settlement period in SK after initial weight gain, whereas food security consistently improved. More detailed understanding of this process is needed to assist healthy settlement for NKR in SK.

• The Korean Journal of Physiology and Pharmacology
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• 제10권1호
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• pp.19-24
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• 2006

The intermediate conductance $Ca^{2+}-activated$ $K^+$ channels (SK4, IKCa1) are present in lymphocytes, and their membrane expression is upregulated by various immunological stimuli. In this study, the activity of SK4 was compared between Bal-17 and WEHI-231 cell lines which represent mature and immature stages of murine B lymphocytes, respectively. The whole-cell patch clamp with high-$Ca^{2+}$ ($0.8{\mu}M$) KCl pipette solution revealed a voltage-independent $K^+$ current that was blocked by clotrimazole (1 mM), an SK4 blocker. The expression of mRNAs for SK4 was confirmed in both Bal-17 and WEHI-231 cells. The density of clotrimazole-sensitive SK4 current was significantly larger in Bal-17 than WEHI-231 cells ($-11.4{\pm}3.1$ Vs. $-5.7{\pm}1.15$ pA/pF). Also, the chronic stimulation of B cell receptors (BCR) by BCR-ligation (anti-IgM Ab, $3{\mu}g$/ml, 8∼12 h) significantly upregulated the amplitude of clotrimazolesensitive current from $-11.4{\pm}3.1$ to $-53.1{\pm}8.6$ pA/pF in Bal-17 cells. In WEHI-231 cells, the effect of BCR-ligation was significantly small ($-5.7{\pm}1.15$ to $-9.0{\pm}1.00$ pA/pF). The differential expression and regulation by BCR-ligation might reflect functional changes in the maturation of B lymphocytes.