• Korean Journal of Microbiology
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• v.51 no.1
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• pp.1-6
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• 2015

The term "P2 sir-associated helper inefficiency" has been used to define the inefficient helper capability of P2 sir mutants for their satellite bacteriophage P4. The aim of this study was to investigate the factors overcoming P2 sir-associated helper inefficiency. At first, we verified whether the P2 cos region containing P4 sid71 cosP2 could overcome P2 sir-associated helper inefficiency with P2 sir3. The result was that P4 sid71 cosP2 could not overcome P2 sir-associated helper inefficiency with P2 sir3. Instead of cos region of P2, the size of the DNA packaged into a $P2_{sir}$-sized head seems to be important for overcoming P2 sir-associated helper inefficiency. In the present work, three kinds of P4 derivatives with packaged DNA sizes between those of P4 ost1 and P4 ost2, were constructed through DNA manipulation. In one P4 derivative, P4 sid71 delRI::apr, the size of the packaged DNA was identified with a CsCl buoyant equilibrium density gradient experiment. According to the burst sizes of the P4 derivatives, they could overcome P2 sir3-associated helper inefficiency. The size of the P4 derivative DNA suitable for packaging into a $P2_{sir3}$-sized head was 28-29 kb.

• Korean Journal of Microbiology
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• v.52 no.1
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• pp.120-124
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• 2016

A certain size of DNA (28-29 kb long) to be packaged into P2-size head and the mutation in sid gene of bacteriophage P4 are the major factors to overcome "P2 sir-associated helper inefficiency". To clarify whether the presence of sid mutation is essential to overcome "P2 sir-associated helper inefficiency" or not, we tested the P4 derivative, P4 delRI::kmr, which is $sid^+$ and whose genome size supposed to be 28.5 kb long in the case of being packaged into $P2_{sir3}$-sized large head. As P4 delRI::kmr showed the low EOP with P2 sir3 lysogen, P4 delRI::kmr phage stock was prepared in P2 sir3 lysogen host to increase the EOP with P2 sir3 lysogen. Through this process, P4 delRI::kmr had been adapted for P2 sir3 lysogen. With a CsCl buoyant equilibrium density gradient experiment and gel electrophoresis of the isolated DNA, it was evident that the adaptation of P4 delRI::kmr for P2 sir3 lysogen was caused by the conformational change of DNA to be packaged into large head. The burst size determination experiments with P4 delRI::kmr phage stock adapted for P2 sir3 lysogen and normal P4 delRI::kmr phage stock showed that not the sid mutation but the size of DNA to be packaged (28-29 kb long) was essential to overcome "P2 sir-associated helper inefficiency".

• Korean Journal of Microbiology
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• v.39 no.4
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• pp.277-282
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• 2003

Bacteriophage P4 ost2 which is the P4 mutant suppressing sir mutations of Bacteriophage P2, was isolated as a plaque-former by plating P4 ash8 sid 71 kmr intS on the lawn of P2 sir3 lysogen. P4 ost2 turned out to be the P4 mutant suppressing sir mutations of P2 in one-step growth experiments.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.30 no.2A
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• pp.51-60
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• 2005

In this paper, we propose an enhanced SIR measurement algorithm for WCDMA mobile station modem. The proposed algorithm minimizes processing delay by applying velocity estimation-based channel estimator with IIR filter and reduces measured SIR offset by compensating attenuated signal power by using pilot channel(CPICH) in fading channel environment. To improve stability of SIR measurement, we also adopt an IIR filter which can properly reflect variation of fading channel in signal and interference power measurement. We prove that the proposed algorithm outperforms conventional SIR measurement algorithm in mean and jitter of measured SIR. Computer simulation shows that performance results using the proposed algorithm have improvement of approximately 8dB for measured mean and 2dB for measured jitter in the wireless mobile channel, especially in fast mobile speed environment.

• Korean Journal of Microbiology
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• v.55 no.2
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• pp.123-130
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• 2019

Chelatase catalyzes the insertion of divalent metal into tetrapyrrole and plays a key role in the biosynthesis of metallated tetrapyrroles, such as cobalamin, siroheme, heme, and chlorophyll. SirB is a sirohydrochlorin (SHC) chelatase that generates cobalt-SHC or iron-SHC by inserting cobalt or iron into the center of sirohydrochlorin tetrapyrrole. To provide structural insights into the metal-binding and SHC-recognition mechanisms of SirB, we determined the crystal structure of SirB from Bacillus subtilis subsp. spizizenii (bssSirB) in complex with cobalt ions. bssSirB forms a monomeric ${\alpha}/{\beta}$ structure that consists of two domains, an N-terminal domain (NTD) and a C-terminal domain (CTD). The NTD and CTD of bssSirB adopt similar structures with a four-stranded ${\beta}-sheet$ that is decorated by ${\alpha}-helices$. bssSirB presents a highly conserved cavity that is generated between the NTD and CTD and interacts with a cobalt ion on top of the cavity using two histidine residues of the NTD. Moreover, our comparative structural analysis suggests that bssSirB would accommodate an SHC molecule into the interdomain cavity. Based on these structural findings, we propose that the cavity of bssSirB functions as the active site where cobalt insertion into SHC occurs.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.23 no.9
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• pp.1019-1024
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• 2012

In this paper, a UWB antenna using a SIR(Step Impedance Resonator) that eliminate signal interference at 5 GHz WLAN as the first rejection band and adjust the second rejection band is proposed. Unlike the unit impedance resonator, the second harmonic of SIR is decided according to step impedance. Therefore, To adjust the second rejection band, SIR is applied to UWB antenna. Also, the equivalent circuit of the antenna at first rejection band is presented and the equivalent modeling values of the SIR and the coupling value is obtained. The proposed antenna is satisfied to cover full UWB band with return losses less than -10 dB and has band rejection characteristic in 5 GHz WLAN band. The radiation patterns show +y directivity characteristics in H-plane and the group delay variations are within 1.0 ns.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.22 no.2
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• pp.245-251
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• 2011

In this paper, dual-band bandpass filter by using Pseudo-Interdigital Stepped-Impedance Resonator(PI-SIR) and Open-Loop Ring Resonator(OLRR) is proposed. The first passband and second passband are formed by PI-SIR and the second passband is reinforced by an OLRR. In a PI-SIR the first band and second band are easily and exactly adjusted by characteristic impedance ratio and electrical length ratio. The proposed design method may be confirmed to be useful from fabricated and measured results for dual-band bandpass filter operated at 2.45 GHz and 5.8 GHz.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.40 no.7
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• pp.282-292
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• 2003

In this paper, we evaluate the performance of WCDMA reverse link receiver system with closed loop fast transmit power control (TPC). For fast power control, SIR must be measured precisely. We propose a new SIR measurement algorithm having a simple structure. The proposed algorithm uses unused OVSF code for interference power evaluation. The proposed SIR measurement algorithm is compared to the conventional SIR measurement algorithm in Ref.$^{［1］}$ under closed loop fast TPC. We adopted WMSA channel estimation filter with Κ=2 for mobile radio channel estimation and considered one slot TPC delay. Extensive computer simulation results show that the proposed algorithm using unused OVSF code reduces the required Ε$_{b}$ /Ι$_{0}$ at the BER of 10$^{-3}$ up to 0.9㏈ and has an improved TPC error performance compared to the conventional algorithm.

• 한국정보통신설비학회:학술대회논문집
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• 2009.08a
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• pp.215-218
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• 2009

In this paper, Dual-band bandpass filter studied design using Parallel Coupled SIR(Stepped Impedance Resonator). This Dual-band bandpass filter design SIR of half-wavelength by Parallel-coupled type that is available to RFID system and Changed structure in Meander form by size reduce. Because seen Dual-band bandpass filter is designed so that is applicable for frequency 433MHz and 2.45GHz of RFID system is very wide distance between two pass-band, establish 433MHz by fundamental frequency and controlled 2.45GHz by 2st spurious resonance frequency bandstop filter of 1st spurious resonance frequency and Parallel coupled SIR Combine to remove 1st spurious resonance frequency.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.18 no.1
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• pp.43-46
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• 2018

This paper has designed a wideband bandpass filter (WBSF : Wide Band Stop Filter) using a stepped impedance resonator (SIR : Stepped Impedance Resonator) with improved performance and improved hairpin coupling structure. The SIR WBSF is small in size and has the advantage of having excellent bandstop characteristics. The designed BSF has a structure in which a quadrangular shaped hairpin of a / 4 length is arranged symmetrically on the upper and lower sides of the input and output transmission lines. The input and output terminals were terminated at 50 ohms for system applications. The center frequency of the SIR WBSF is 6.3 GHz, which is the second harmonic of 3.15 GHz. The designed filter has a 3dB bandwidth of 2.9 GHz and a transmission coefficient ($S_{21}$) of 33.2 dB. The reflection coefficient ($S_{11}$) at the center frequency is 0.106 dB. The application field is used for fixed microwave relay stations, fixed satellite and earth stations, and fixed satellite communications. The overall size is $20mm{\times}10mm$.