• Journal of Physiology & Pathology in Korean Medicine
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• v.18 no.4
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• pp.1055-1060
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• 2004

Apoptosis is the ability of cells to self-destruct by the activation of an intrinsic cellular suicide program when the cells are no longer needed or when they are seriously damaged. Morphologically, apoptosis is characterized by the appearance of membrane blebbing, cell shrinkage, chromatin condensation, DNA cleavage, and the fragmentation of the cell membrane-bound apoptotic bodies. Siegesbeckia glabrescens Makino (Siegesbeckiae Herba, SG) has been widely used as treatments for arthritis, and fever, as well as detoxification properties. The present studies were undertaken to evaluate if SG has an anti-apoptotic property. Cell viability was measured by XTT and tryphan blue stain. Morphological characteristic of human aortic smooth muscle cells(HASMC) were visualized with a phase-contrast microscope. SG significantly reduced HASMC, but not human umbilical vein endothelial cell(HUVEC), viability in a dose-dependent manner. Confluent untreated cells at 24hrs showed normal morphology, flat with a uniform polygonal shape. SG-treated cells (0.5㎎/㎖) at 24hrs showed apoptotic morphology. Cells became irregular with elongated lamellipodia, and exhibited condensed chromatin in nuclei with occasional endoucleation. There was an increase in the number of apoptotic cells rounding-up and being detached from the substrate. TUNEL staining of SG-treated cells showed dark-brown stains in nuclei and cytosol. Caspases are central components of the machinery responsible for apoptosis and are generally divided into two categories; the initiator caspases, which include caspases-2,-8,-9, and -10, and the effector caspases, which include caspases-3,-6, and -7. SG decreased anti-caspase-3 protein expression, which means activation of caspases-3 activity. It has been reported that there is a link between NO formation and apoptosis. NO production was accelerated by SG treatment in HASMC. L-NNA, NOS inhibitor, inhibited SG-induced apoptosis. These results, therefore, indicated that both caspases-3 and NO production are involved in apoptosis in smooth muscle cells. According to these results, SG may have a potential effect in the treatment of hypertensive atherosclerosis.

• Journal of Yeungnam Medical Science
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• v.8 no.1
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• pp.42-52
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• 1991

Two types of urine dipstick assays, Multistix-SG and Combur-9-test RL, were compared for compatibility, accuracy, specificity and predictive values of a positive and negative test in 501 patients urine and artificially prepared specimen. We found that the results of semiquantitative tests of Multistix-SG and Combur-9-Test RL performed were statistically similar in patients specimen. The urinary leukocyte estrase tests of Combur-9-Test RL assays compared with urine sediment microscopy in regard to compatibility, sensitivity, specificity, and predictive values of a positive and negative test 83.7%, 48.1%, 90.3%, 47.4% and 90.1%, respectively. The urinar nitrite tests of Comber-9-Test RL assays compared with urine culture tests, in regard to compatibility, sensitivity, specificity, and predictive values of a positive and negative tests were 90.3%, 19.4%, 84.7%, 53.8% and 94.1%, respectively. For the urinary protein, the sulfosalicylic acid method was the most sensitive test for any kinds of protein, and Multistix-SG appeared more sensitive than Compur-9-Test RL for the albuminuria. For the urinary bilirubin and glusose, two dipstick assays were similar in their diganostic efficiency. Finally in the urinary occult blood tests, Combur-9-Test RL assays was more sensitive than Multistix-SG.

• Korean Journal of Acupuncture
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• v.40 no.3
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• pp.90-98
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• 2023

Objectives : Parkinson's disease (PD) is the second most common neurodegenerative disorder worldwide and is characterized by the loss of the dopaminergic neurons in the substantia nigra (SN). In a previous in vitro study, we demonstrated that Sihogyeji-tang (SG), Sihosogan-tang (SS), and Sihocheonggan-tang (SC) have the potential to be candidate medicines for PD. This study aimed to compare the neuroprotective effect of SG, SS, and SC using 1-methyl-4-phenyl-1,2,3,6-tetrahydrophridine (MPTP)-induced PD mouse model. Methods : Eight-week-old male C57BL/6 mice were intraperitoneally administered with 30 mg/kg of MPTP for 5 days and orally administered SG, SS and SC for 12 days from the first MPTP injection. Motor function was assessed using the pole test and the rotarod test. Dopaminergic neuronal survival in the SN and striatum was evaluated through tyrosine-hydroxylase immunohistochemistry. Results : MPTP administration resulted in behavioral impairment and dopaminergic neuronal death in the SN and striatum. In the pole test, treatment with SG, SS, and SC alleviated the MPTP-induced motor dysfunction on day 5 and 12. In the rotarod test, SS and SG alleviated the MPTP-induced motor dysfunction on day 5, while only SS showed improvement on day 12. SS and SG significantly protected dopaminergic neurons in the SN from MPTP toxicity, and all three compounds (SG, SS, and SC) showed significant protection in the striatum. Notably, SS demonstrated superior efficacy in suppressing MPTP-induced motor dysfunction and dopaminergic neuronal death compared to SG and SC. Conclusions : These findings suggest that SS is the most effective formula among SG, SS, and SC for PD, indicating its potential role in the treatment of PD.

• IEIE Transactions on Smart Processing and Computing
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• v.1 no.1
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• pp.34-49
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• 2012

Recently, the smart grid (SG) has been introduced to solve the serious network issues caused by the increasing electrical demand and the complex nonlinear nature of the electric power distribution network. The SG, regarded as the next generation power grid, can transmit power in more efficient ways by building an automatic and distributed energy delivery network. In this paper, we first assess how various existing networking technologies, such as IEEE 802.11 (WiFi) and IEEE 802.15.4 (ZigBee), meet the requirements of the SG communication protocols. Specifically, we classify the existing network protocols into three categories: WSN-based networking, WiFi-based networking, and wireline-based networking. We then survey the security issues regarding the SG. Finally, we propose an agile SG networking architecture and show the effectiveness of different adopted networking technologies and, as a result, present a candidate solution to implement agile networking in SGs.

• The Journal of the Korea institute of electronic communication sciences
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• v.5 no.4
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• pp.418-424
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• 2010

Many researchers studied methods for the generation of maximum length pseudo random sequences. Sabater et al. analyzed shrunken sequences which are effectively generated by SG(Shrinking Generator) using CA(Cellular Automata). In this paper we propose a new SG which is called LCSG(LFSR and CA based Shrinking Generator) using an LFSR with control register and CA with generator register. The proposed shrunken sequences generated by LCSG have longer periods and high complexities than the shrunken sequences generated by the known method. And we analyze the generated sequences using LCSG.

• ETRI Journal
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• v.28 no.1
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• pp.59-66
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• 2006

Learning behaviors of a radial basis function network (RBFN) using a singular value decomposition (SVD) and stochastic gradient (SG) algorithm, together named RBF-SVD-SG, for odor sensing systems are analyzed, and a fast training method is proposed. RBF input data is from a conducting polymer sensor array. It is revealed in this paper that the SG algorithm for the fine-tuning of centers and widths still shows ill-behaving learning results when a sufficiently small convergence coefficient is not used. Since the tuning of centers in RBFN plays a dominant role in the performance of RBFN odor sensing systems, our analysis is focused on the center-gradient variance of the RBFN-SVD-SG algorithm. We found analytically that the steadystate weight fluctuation and large values of a convergence coefficient can lead to an increase in variance of the center-gradient estimate. Based on this analysis, we propose to use the least mean square algorithm instead of SVD in adjusting the weight for stable steady-state weight behavior. Experimental results of the proposed algorithm have shown faster learning speed and better classification performance.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.30 no.12 s.255
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• pp.1618-1625
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• 2006

During the last two decades, several guidelines have been developed and used for assessing the integrity of a defective steam generator (SG) tube that is generally caused by stress corrosion cracking or wall-thinning phenomenon. However, as some of SG tubes are also failed due to fretting and so on, alternative failure estimation schemes are required for relevant defects. In this paper, parametric three-dimensional finite element (FE) analyses are carried out under internal pressure condition to simulate the failure behavior of SG tubes with different defect configurations; elliptical wear, tapered and flat wear type defects. Maximum pressures based on material strengths are obtained from more than a hundred FE results to predict the failure of SG tube. After investigating the effect of key parameters such as defect depth, defect length and wrap angle, simplified failure estimation equations are proposed in relation to the equivalent stress at the deepest point in wear region. Comparison of failure pressures predicted by the proposed estimation scheme with corresponding burst test data showed a good agreement.

• Journal of Oriental Neuropsychiatry
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• v.21 no.4
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• pp.41-51
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• 2010

Objectives : Samul-tang(SM). Sagunja-tang(SG). Pamul-tang(PM) and Sipjeondaebo-tang(SJ) was used many diseases such as sterility. menstrual disorder. general prostration. recruitment in Korea. We investigated the immune stimulatory activities of SG. SM. PM and SJ in in vitro. Methods: For comparision for effective of SM. SG. PM and SJ. this study examined anti-inflammation(NO. PGE2 assay). anti-oxidation(DPPH assay) and immune response in in vitro assay. For immune response activities. this study used NO synthesis on RAW 264.7 cells, splenocyte proliferation and cytokine assay(IL-2, IL-4) in splenocyte. Results: The results showed that SG. SM. PM and SJ were no significant effect anti-oxidation and anti-inflammatory effects. For immune response. they showed the splenocyte proliferation and macrophage proliferation. We confirmed that they synthesised NO a dose-dependent manner significantly and secreted the IL-4. Conclusions : These results suggested the SG, SM, PM and SJ had immune stimulatory activity. A efficacy of immune response in them had produced similar results.

• The Plant Pathology Journal
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• v.17 no.3
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• pp.115-122
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• 2001

Potato virus X (PVX) is a flexuous rod-shaped virus containing a single plus-strand RNA. Viral RNA synthesis is precisely regulated by regulatory viral sequences and by viral and/or host proteins. RNA sequence element as well as stable RNA stem-loop structure in the 5' end of the genome affect accumulation of genomic RNA and subgenomic RNA (sgRNA). The putative sgRNA promoter regions upstream of the PVX triple gene block (TB) and coat protein (CP) gene were critical for both TB and CP sgRNA accumulation. Mutations that disrupted complementarity between a region at the 5' end of the genomic RNA and the sequences located upstream of each sgRNA initiation site is important for PVX RNA accumulation. Compensatory mutations that restore complementarity restored sgRNA accumulation levels. However, the extent of reductions in RNA levels did not directly correlate with the degree of complementarity, suggesting that the sequences of these elements are also important. Gel-retardation assays showed that the 5' end of the positive-strand RNA formed an RNA-protein complex with cellular proteins, suggesting possible involvement of cellular proteins for PVX replication. Future studies on cellular protein binding to the PVX RNA and their role in virus replication will bring a fresh understanding of PVX RNA replication.

• Korean Journal of Materials Research
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• v.13 no.2
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• pp.74-80
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• 2003

The effects of risering design and alloying element on the formation of defects such as external depression, primary and secondary shrinkage cavities in ductile cast iron were investigated. Two types of risering design for the cylindrically step-wise specimen, No. 1(progressive solidification) and No. 2(directional solidification) risering designs, were prepared and six different alloy compositions were casted. In the No. 1 risering design, external depression or primary shrinkage cavities due to liquid contraction were observed in all the specimens from SG 10 to SG 60. The defects caused by liquid contraction seemed to be more affected by risering design than alloying elements. The secondary shrinkage cavities were also observed in all the specimens but a swollen surface was not observed in all the castings. The primary shrinkage cavities were located right under the top surface or connected to the top surface, and were characterized by smooth surfaces. On the other hand, the secondary shrinkage cavities were positioned in the thermal center of the specimen steps 3 and 4, and characterized by rough surfaces. In the No. 2 risering design, no external depression or primary shrinkage cavities due to liquid contraction were observed in all the specimens from SG 10 to SG 60. However, the secondary shrinkage cavities were formed in the thermal center of specimens SG 40, 50 and 60. Like the No. 1 risering design, a swollen surface was not observed in all the castings.