• 한국가금학회지
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• 제21권2호
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• pp.133-138
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• 1994

A study of concurrent bioassay for protein quality and energy level in protein sources was rnade by determining urinary nitrogenous compounds in excreta. The carry over effect of previous feeding was eliminated by 48 h of feeding the experimental diets prior to the determination of for protein digestibility and utilizability, and energy digestibility and metabolizability at 24 h interval during 3 days. Then, protein qualities and energy levels for soybean meal, rapeseed meal and fish meal were calculated by a substitution method. Apparent protein utilization (NB/NI) was affected by the increased fecal nitrogen excretion in soybean meal and by the increased urinary nitrogen excretion in rapeseed meal and fish meal. The apparent metabolizability of energy (ME/GE) was affected by the fecal energy excretion in soybean meal and rapeseed meal and by urinary energy excretion in fishmeal. The results indicated that the concurrent bioassay of protein quality and energy levels in ingredients appears to be applicable to chickens of other age, sex, breeds and environmental conditions.

• 한국수정란이식학회지
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• 제10권1호
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• pp.91-100
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• 1995

3.3kb 웅성특이 DNA(pEM39 plasmid DNA)가 성 특이 DNA 검색자로 활용되어질 수 있는가를 확인하기 위하여 구조적인 분석을 Southern blotting, DNA sequencing과 computer program 분석을 통하여 실시하였다. 전체 3.3kb에서 유래된 약 1kb 단위의 단편을 이용하여 표지된 짧은 DNA probe들은 Southern blot 분석에서 웅성특이성을 나타내었다. McGraw와 Jeon의 sequence에 대한 유사성 비교 자료로부터 여러 부분의 conserved region을 찾아내고 이것을 기초로 하여 5개의 primer set들을 선발하였다. Conserved region에 존재하면서 computer program에 의해서 선발되어진 PMS1과 2의 primer set가 최종적으로 PCR 분석을 위하여 선정되었다. 이 primer set를 사용한 PCR 분석에서, 1ng부터 10pg까지의 웅성 genomic DNA에서 PCR 산물을 얻을 수 있었으며, 자성의 경우는 어떠한 산물도 찾을 수 없었다. PCR에 이용할 수정란의 시료는 2 세포기의 수정란에서 얻었으며 순수 분리된 genomic DNA에서 확립된 조건에서 PCR을 수행하였다. 8개의 수정란을 분석한 결과 4개의 웅성과 4개의 자성 수정란을 확인하였다. 이러한 결과는 선정된 primer set가 돼지 수정란의 성을 조기 감별하는데 효율적인 DNA probe로 사용될 수 있다는 것을 암시한다.

• 환경위생공학
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• 제2권2호
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• pp.1-22
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• 1987

Measurements of kidney, liver, lung along with blood trace metal levels were obtained in 20 exposed and 40 control cattle. The level of cadmium, mercury and arsenic were the highest in the kidney while copper, lead and zinc concentrations were the highest in the liver. Statistically significant correlation between the kidney and the liver were found for cadmium, copper, zinc, mercury and arsenic. No significant differences were observed between the area, sex and age in this study. The levels found were generally within the ranges compared with those found in similar studies in and outside.

• 보존과학연구
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• 통권24호
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• pp.153-167
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• 2003

We performed nuclear DNA typing and mitochondrial DNA sequencing analysis based on PCR from an ancient Korean remainsexcavated from Siheung in Korea. 7 bones were collected and partially STR(short tandem repeat) systems, Sex determination Amelogenin kit(Promega co, USA), were used in this study. Mitochondrial DNAs were also amplified and sequenced by ABI 310 DNA sequencer. We know that sample no. 2 and no. 3 were females and also sample no. 2 and no.7 possessed the same maternal inheritance by mitochondrial DNA sequencing results. Throughout this research, the mitochondrial DNA sequencing of human in the middle of Joseon Dynasty in Korea is obtained. In addition, this finding will be an important foundation for the future research.

• Child Health Nursing Research
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• 제10권4호
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• pp.431-441
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• 2004

Purpose: The purpose of this study was to develop a sexuality education course of study for students in the lower grades of elementary school. Method: On the basis of the sexuality education guidebook for elementary school students published by Korea Ministry of Education & Human Resources Development, the state-designated textbooks for first to third grade were analyzed. Results: 1) In the category of physical and psychological development, physical development and psychosexual development content were covered, but not sexual health content. 2) In the category of understanding of human relationship, marriage and family, and the opposite sex and love were presented but self-determination and selection were only partially presented. 3) In the category of sexual culture and sexual ethics, sexual role in society was covered, but not social environment or equality of the sexes. Conclusion: These results suggest that content on sexuality which is not covered in textbooks for students in lower grades should be included in other school courses or activities in class.

• 한국조경학회지
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• 제21권3호
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• pp.99-104
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• 1993

The objectives of this study were investigation of the sutiable propagation method by softwood cuttings of female Ilex serrata, the comparison of sex determination of Ilex serrata after seeding and the fruiting percentage of the Ilex serrata male and female. The results obtained were as follows. 85 percentage of the seed were germinated with three months cold stratification. 35 percentage of female plant were observed after seeding. Cuttings should be rooted on late June using a quick dipping of 1,000ppm IBA solution. The rooting rate of the softwood cuttings treated IBA 1,000pm on June 22 in a closed moist chamber with 50% shade screen was 72.2 percentage. The suitable planting of male and female of Ilex serrata was male one versus female thirteen in a distance of 50 meter.

• Fisheries and Aquatic Sciences
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• 제27권2호
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• pp.57-65
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• 2024

Paiche (Arapaima gigas), is a colossal freshwater fish native to the Amazon basin. Its geographic distribution spans various regions, including Brazil, Peru, Colombia, and Guyana, making it a significant component of the aquatic ecosystems in this area. Beyond its ecological role, the paiche holds substantial importance as a valuable fish resource for local communities, providing sustenance and economic opportunities. This review provides a comprehensive analysis of the reproductive aspects of the paiche, based on information published from January 2000 to January 2022. It encompasses a wide range of reproductive characteristics, including sexual differentiation, age at first maturity, and identification techniques. Additionally, it offers an evaluation of various mating behaviors, highlighting their respective advantages and disadvantages. The review also explores genetic and behavioral traits observed in both wild and captive specimens, offering valuable insights for the effective management of breeding programs.

• Fisheries and Aquatic Sciences
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• 제1권1호
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• pp.82-93
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• 1998

Monthly changes of the gonad follicle index (GFI), reproductive cycle, egg-diameter composition, first sexual maturity of the Pacific oyster, Crassostrea gigas, were studied based on the samples which have been collected from the intertidal zone of Poryong west coast of Korea, from January to December, 1996. C. gigas, is dioecious, while a few individuals are alternatively hermaphroditic. Monthly variation of gonad follicle index (GFI) used for determination of spawning period, coincided with the reproductive cycle. GFI increased from April when seawater temperatures gradually increased and reached the maximum in May. And then, GFI sharply decreased from June to September due to spawning. Reproductive cycle of this species can be divided into five successive stages: in females, early active stage (March to April), late active stage (April to May), ripe stage (May to August), partially spawned stage (June to September) and spent/inactive stage (September to February); in males, early active stage (February to March), late active stage (April to May), ripe stage (May to September), partially spawned stage (June to September) and spent/ inactive stage (September to February). The diameter of fully mature eggs are approximately 50um. Spawning occurred from June to September, and two spawning peaks were observed in June and August when the seawater temperature was above $20^{\circ}C$. Percentages of the first sexual maturity of males of 20.1-25.0 mm in shell height were over $50\%$, while those of females of 25.1-30.0 mm in shell height were over $50\%$. All the males of > 30.1 mm and all the females of ^gt; 35.1 mm completed their first sexual maturity. The results suggest that C. gigas has a protandry phenomenon. Sex ratios of 919 oysters observed were 453 females $(49.29\%)$, 429 males $(46.68\%)$, 16 hermaphrodites $(1.74\%)$, and 21 indeterminate individuals $(2.29\%)$. In age class I, sex ratio of males were $64.00\%$, thus, a higher percentage than that of females. It was noted that $64.00\%$ of the young males (age class I) were more functional than females in age class I, but 2-3 year-old oysters showed higher percentage of females. Percentages of hemaphrodites in 2-3 year classes were relatively higher than those in other year classes. Histological pattern of hermaphrodites can be divided into two types: Type I (hermaphrodite having a number of newly formed developing oocytes on the oogenic tissues within a degenerating spermatogenic follicle after discharge of numerous spermatozoa) and Type II (hermaphrodite having two separate follicles in the same gonad).

• 한국수산과학회지
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• 제39권3호
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• pp.297-302
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• 2006

황점볼락, Sebastes oblangus의 인공종묘의 성분화 과정 및 성결정을 조사하기 위해 출산 직후부터 370일령까지 조사하였다. 출산 직후 난황기 자어(7.10-7.77 mm)에서 시원생식세 포와 생식융기는 분리된 채 중신관과 장간막 사이에 나타났고, 출산 후 5일령 전기자어(7.12-9.68 mm)에서는 서로 융합하였으며, 출산 후 45일령 후기자어(18.6-20.4 mm)까지는 미분화 생식소 상태였다. 출산 50일 치어(20.0-24.5 mm)에서 생식소 전단부에서 양쪽 끝의 체세포 조직이 분열 신장되어 난소의 분화가 시작되어 60일 치어(25.5-32.0 mm)에서 완전 한 난소강이 형성되었다. 출산 80일령 치어(37.3-47.2 mm)의 난소에서 난원세포가 감수분열을 개시하여 염색인기 난모세포(chromatin nucleolus oocyte) 로 발달하기 시작하였다. 이 후 일령이 증가하여 130일령(68.0-86.0 mm)에서 주변인기(perinucleolus stage)의 난모세포가 나타났으며, 출산 후 370일령(101.0-116.0 mm)에서 전난황형성기의 난모세포들이 출현하였다. 황점볼락 인공종묘의 성비 조사결과 난소분화 완료 후에 암컷만 나타나 출산후 100일까지 자연수온보다 높은 수온에 의해 전암컷화 된 것으로 추정된다.

• 보존과학회지
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• 제30권4호
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• pp.409-416
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• 2014

유적지에서 발굴되는 조선시대 인골은 보존 상태가 대부분 양호하여 형질인류학, 유전학, 그리고 화학적 연구를 수행할 수 있다. 본 연구에서는 김포 장기동 유적지에서 발굴된 조선시대 인골 6개체에 대한 DNA 분석을 통하여 미토콘드리아 DNA 변이형을 결정하였으며, 성별 분석결과를 토대로 남성 피장자의 Y 하플로그룹을 동정하였다. 유전자 분석에 앞서 보존상태가 양호한 것으로 판단되는 인골 6구를 대상으로 조직학 지수를 판별하였다. 미토콘드리아 DNA 변이형은 아시아인에서 높은 빈도로 나타나는 G, R11, M7, A5 등의 하플로그룹이 확인되었다. 인골의 성별을 확인하기 위하여 아밀로제닌 유전자 분석을 수행한 결과, 6구의 인골에서 여성 4구와 남성 2구의 성별을 확인할 수 있었다. 남성으로 판명된 인골에 Y 염색체 단일염기다형성 분석을 적용해본 결과, 하플로그룹 O로 확인되었다. 향후 출토 인골의 광범위한 분석을 위하여 인골을 선별한 경우에는 본 연구에서 제시한 조직학 지수를 적용하고, 미토콘드리아 DNA와 핵 DNA의 다양한 분석을 병행하여 출토 인골간의 유연관계를 규명하는 것이 필요하다.