• Title/Summary/Keyword: SEB-1

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Detection of Staphylococcus aureus and Screening Staphylococcal Enterotoxin a, b, c genes in Strains Isolated from Strawberry Juice Shops in Jinju (진주 지역 딸기 주스 상점에서의 Staphylococcus aureus의 분리와 staphylococcal enterotoxin a, b, c gene 검색)

  • Kim, Se-Ri;Park, Seon-Ja;Shim, Won-Bo;Kim, Hyoung-Kab;Chung, Duck-Hwa
    • Journal of Environmental Health Sciences
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    • v.31 no.1
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    • pp.23-30
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    • 2005
  • Staphylococcus aureus is one of the important pathogenic agents, which are related to the hygienic condition. This study performed for the detection of Staphylococcus aureus and screening staphylococcal enterotoxin a, b, c genes in strains isolated from the environment for production of non-pasteurized strawberry juice. A total of 44 samples were collected from utensils, machinery, employees, raw materials, and strawberry juices in 3 strawberry juice shops in Jinju, western Gyeongnam. The isolation rate of Staphylococcus aureus was 26%. Specially Staphylococcus aureus was frequently isolated from employee's hands, strawberry and strawberry juices. The sea, seb, and sec genes were also investigated by polymerase chain reaction (PCR). One hundred and 55% of each isolate had found sea gene and seb gene, respectively. However, sec gene was not detected anywhere. To prevent food-borne disease associated with juice, the accomplishment of HACCP to be more efficient and systematic is necessary.

Preparation and Properties of SEBS (Styrene Ethylene Butadiene Styrene Copolymer)-Clay Hybrid Gas Barrier Membranes (Gas Barrier성 SEBS (Styrene Ethylene Butadiene Styrene Copolymer)-Clay 하이브리드 막의 제조 및 물성)

  • Nam Sang Yong;Yeom Bong Yeol;Min Byoung Ryul;Kim Young Jin
    • Membrane Journal
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    • v.15 no.1
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    • pp.62-69
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    • 2005
  • SEBS-clay hybrid membranes were prepared by melt intercalation method with internal mixer. In the hybrid, the amount of clay content was fixed to 5 phr. MMT was intercalated or exfoliated by the ionomer and it was confirmed by X-ray diffraction method. D-spacing of the characteristic peak from MMT plate in SAXD was moved and diminished. Gas permeability, mechanical properties and thermal properties of the SEBS-clay hybrid membranes were investigated. Gas permeability through the SEBS-clay hybrid membranes decreased due to increased tortuosity made by intercalation of clay in SEBS.

Profiles of Toxin genes and Antibiotic Susceptibility of Staphylococcus aureus Isolated from Perilla Leaf Cultivation Area (들깻잎 재배단지에서 분리한 Staphylococcus aureus의 독소 유전자와 항생제 감수성 분석)

  • Kim, Se-Ri;Cha, Min-Hee;Chung, Duck-Hwa;Shim, Won-Bo
    • Journal of Food Hygiene and Safety
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    • v.30 no.1
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    • pp.51-58
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    • 2015
  • Thirty one of Staphylococcus aureus isolated from perilla leaf cultivation areas in Miryang were investigated on the characteristics, such as enterotoxin genes and antibiotic susceptibility. Five toxin genes (sea, seb, sec, sed, and see) were examined by PCR method. Disc diffusion method was used to examine the antibiotic susceptibility of S. aureus by using 18 types of antibiotic discs with different concentrations. Among enterotoxin-encoding genes, sea and sed genes were co-detected from 4 isolates (12.9%), sed gene was founded in 9 isolates (29.0%), and see gene was founded in 1 isolate (3.2%). However seb and sec and tsst were not detected in any isolates. As a result of antibiotic susceptibility test, 7 isolates (22.6%) were resistant to 12 antibiotics (penicillin, ampicillin, oxacillin, amoxicillin-clavulanic acid, cefazolin, cephalothin, imipenem, gentamicin, tetracycline, ofloxacin, norfloxacin, and erythromycin). 2 isolates (6.5%) were resistant to 5 antibiotics (penicillin, ampicillin, amoxicillin-clavulanic acid, gentamycin, and telithromycin). MRSA (Methicilline Resistant Staphylococcus aureus) was founded in packing vinyl, hands, and perilla leaves.

Effect of Compatibilizer Types on the Properties of Linear PPS/PET Blends (상용화제의 종류가 선형 PPS/PET 블렌드의 물성에 미치는 영향)

  • Kim, Sungki;Hong, In-Kwon;Lee, Sangmook
    • Polymer(Korea)
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    • v.37 no.4
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    • pp.500-506
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    • 2013
  • The effect of compatibilizer types on the properties of polyphenylene sulfide (PPS)/polyethylene terephthalate (PET) blends was investigated. The blends were extruded by a single screw extruder attached with a Maddock mixing zone and their molded properties were examined. As a basic blend composition, a linear PPS/PET (40/60) blend was selected based on cost efficiency. Three types of compatibilizer, SEBS, modified SEBS, and modified PS were added to the basic blend to improve the properties. The thermal, rheological, mechanical properties and the morphology of the ternary blends were analyzed. The maximum mechanical properties of blends was found at 1 phr of m-SEBS or m-PS content, whose values were almost the same as the theoretical values of miscible blend system. It seemed to by the case that the partial reaction between compatibilizer and the basic blend caused the enhancement of compatibility between linear PPS and PET phases. These ternary blends would be applicable as economic linear PPS alloys.

Influence of silver nanoparticles on resin-dentin bond strength durability in a self-etch and an etch-and-rinse adhesive system

  • Jowkar, Zahra;Shafiei, Fereshteh;Asadmanesh, Elham;Koohpeima, Fatemeh
    • Restorative Dentistry and Endodontics
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    • v.44 no.2
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    • pp.13.1-13.9
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    • 2019
  • Objectives: This study evaluated the effect of dentin pretreatment with silver nanoparticles (SNPs) and chlorhexidine (CHX) on the microshear bond strength (${\mu}SBS$) durability of different adhesives to dentin. Materials and Methods: Occlusal surfaces of 120 human molars were ground to expose flat dentin surfaces. The specimens were randomly assigned to six groups (n = 20). Three groups (A, B, and C) were bonded with Adper Single Bond 2 (SB) and the other groups (D, E, and F) were bonded with Clearfil SE Bond (SEB). Dentin was pretreated with CHX in groups B and E, and with SNPs in groups C and F. The specimens were restored with Z250 composite. Half of the bonded surfaces in each group underwent ${\mu}SBS$ testing after 24 hours and the other half was tested after 6 months of water storage. Results: SNP application was associated with a higher ${\mu}SBS$ than was observed in the CHX and control groups for SEB after 24 hours (p < 0.05). A significantly lower ${\mu}SBS$ was observed when no dentin pretreatment was applied compared to dentin pretreatment with CHX and SNPs for SB after 24 hours (p < 0.05). The ${\mu}SBS$ values of the 6-month specimens were significantly lower than those obtained from the 24-hour specimens for all groups (p < 0.05). This decrease was much more pronounced when both adhesives were used without any dentin pretreatment (p < 0.05). Conclusions: SNPs and CHX reduced the degradation of resin-dentin bonds over a 6-month period for both adhesive systems.

Thermal Analysis of LaNi5 Hydride by Volumetric Method (부피법 자동장치를 이용한 LaNi5 수소화합물의 열분석 장치개발)

  • HAN, JEONG-SEB;KIM, SUNJUNG
    • Journal of Hydrogen and New Energy
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    • v.29 no.1
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    • pp.25-31
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    • 2018
  • To apply Sievert's type apparatus to thermal analysis of hydrogen absorption materials, the dehydrogenation of $LaNi_5$ system was investigated. As the initial wt% of hydrogen was increased from 0.44 to 1.24 wt%, the peak temperature of evolution rate shifted to higher temperature. However, with the initial wt% of hydrogen higher than 0.95 wt%, the peak temperature of evolution rate did not change. As the heating rate was increased, the peak temperature increased; the peak temperatures for heating rates 0.5, 1.0 and 1.5 K/min were 262.2, 264.1, and 265.9 K respectively. The Sievert's type automatic apparatus can be successively applied to the thermal analysis of $LaNi_5$ hydride.

Spatial Information Transfer with a Stationary Coupling Wave in Rb Atoms

  • Bae, In-Ho;Moon, Han-Seb
    • Journal of the Optical Society of Korea
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    • v.12 no.3
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    • pp.192-195
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    • 2008
  • We report on the spatial information of a coupling laser transfer into a reflected probe laser; the transfer is achieved by means of electromagnetically induced transparency through a common excited state connected with $5S_{1/2}-5P_{1/2}(F=1{\rightarrow}F'=2)$ in the $^{87}Rb\;D_1$ line. When the coupling laser was spatially modulated as a stationary wave, the absorption of the probe laser was enhanced and the reflection of the probe laser was generated. When the coupling laser was spatially modulated by a mask, we observed that the reflection light of the probe laser was modulated as the shape of the mask. The Bragg reflection transferred the spatial information of the coupling laser. The reflection was approximately 7% of the incident power of the probe laser.

Transient Response Analysis and Compensation of the Second Order System with OIne PHP Real Zero

  • Byung-Moon kwon;Ryu, Hee-Seb;Kwon, Oh-Jyu
    • Transactions on Control, Automation and Systems Engineering
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    • v.2 no.4
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    • pp.262-267
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    • 2000
  • In this paper, the magnitude of undershoot and overshoot in a prototype second order system with one positive real zero is computed by the analytic methods. Also, it will be shown that the peak times of the undershoot and overshoot can be calculated using the impulse and step response of the second order system. Three different cases are investigated: underdamped(p<ζ<1), critically damped(ζ=1) and overdamped(ζ>1) cases. We deal with the undamped(ζ=0) case as a special case of the underdamped. And a compensation method is proposed to reduce undershoots of the nonmininmun phase system using feedforward compensator.

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Cannabidiol Inhibits Lipogenesis by Regulating Akt/AMPK-SREBP-1 Pathway in Sebocytes (피지세포에서 Akt/AMPK-SREBP-1 경로를 통한 CBD의 피지 합성 억제 효능)

  • Yoon Gyung Kwon;Ji Young Yoon;Hanon Lee;Dong Hyo Kim;Jun Hyo Lee;Diane M Thiboutot;Dae Hun Suh;Byoung Jun Park
    • Journal of Life Science
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    • v.33 no.4
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    • pp.343-348
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    • 2023
  • Acne is one of the most common skin diseases, mainly occurring in adolescence. The pathophysiology of acne involves not only hormonal, genetic and environmental factors, but also other factors including hyperseborrhea, inflammation, over-keratinization of follicular keratinocytes and overgrowth of Cutibacterium acnes (C. acnes). Cannabidiol (CBD) is known to relieve pain, stress and inflammation. Moreover, cannabis extracts containing CBD have been reported to be effective in treating acne. However, the therapeutic effect of CBD on acne remains unclear. Therefore, this study aimed to investigate the effect and mechanism of CBD on lipogenesis in SEB-1 sebocytes. We treated sebocytes with CBD and found that it not only inhibited lipid synthesis, but also inhibited cell proliferation by inducing apoptosis. We then demonstrated that sterol response element-binding protein-1 (SREBP-1) mediates the inhibitory effect of CBD on lipogenesis. Furthermore, Akt and adenosine monophosphate-activated protein kinase (AMPK), upstream regulators of SREBP-1, were regulated by CBD treatment. Taken together, our studies demonstrate that CBD inhibits adipogenesis by regulating the Akt/AMPK-SREBP-1 signaling pathway, providing potential for use as a therapeutic agent for acne. Further research is needed to confirm the effect of CBD on inflammation caused by hyperkeratosis, which will increase the possibility of using CBD for acne treatment.

Thermal Analysis of Mg2Cu Hydride (Mg2 hydride의 열분석)

  • Han, Jeong-Seb
    • Journal of Hydrogen and New Energy
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    • v.2 no.1
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    • pp.1-6
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    • 1990
  • The desorption kinetics of $Mg_2Cu$ hydride were studied by thermal analysis technique in order to study desorption behavior and to relate thermal desorpton spectra to occuption site of hydrogen. It is suggested that a continuous ${\alpha}/{\beta}$ interface boundary is formed at the initial absorption stage. And the desorption kinetics were analysed by the theoretical equation which was derived on the basis of continous moving boundary model. The number of thermal desorption peak corresponds to the occupation sites of hydrogen. The apparent activation energy for the desorption of $Mg_2Cu$ hydride is 91 KJ/mol.

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