• The Journal of Pediatrics of Korean Medicine
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• v.30 no.2
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• pp.96-106
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• 2016

Objectives The purpose of this study is to investigate the effects of Gleditsiae Fructus 70% EtOH extract (JS_E) on anti-inflammatory action in HaCaT cells (A spontaneously immortalized human keratinocyte cell line) and inhibiting triglyceride genesis in SEB-1 cells (Immortalized human sebocyte). Methods The anti-inflammatory effect of JS_E was analyzed by enzyme-linked immunosorbent assays (ELISA) which measured levels of IP-10, RANTES and MDC in HaCaT cells. Also the effect on secretion of sebum of JS_E was analyzed by TG-S kit which measured the quantity of triglyceride in SEB-1 cells. Results JS_E inhibited IP-10, RANTES and MDC expression in a dose dependent manner. IP-10 expression was inhibited significantly in comparison to TNF-${\alpha}$ and IFN-${\gamma}$ recombination (TI) control group at concentration of JS_E $200{\mu}g/ml$ and RANTES and MDC expressions were inhibited significantly at concentration of JS_E 100, $200{\mu}g/ml$. JS_E also inhibited triglyceride secretion of SEB-1 cells significantly in comparison to the control group in a dose dependent manner. Conclusions This study shows that JS_E has the effects of anti-inflammatory action and inhibiting sebum secretion. According to these results, JS_E can be used for treating skin diseases such as acne and dermatitis caused by inflammation and excessive secretion of sebum by controlling the activity of the HaCaT and SEB-1 cells.

• IMMUNE NETWORK
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• v.22 no.2
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• pp.15.1-15.16
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• 2022

Foreign molecules, including viruses and bacteria-derived toxins, can also induce airway inflammation. However, to the best of our knowledge, the roles of these molecules in the development of airway inflammation have not been fully elucidated. Herein, we investigated the precise role and synergistic effect of virus-mimicking double-stranded RNA (dsRNA) and staphylococcal enterotoxin B (SEB) in macrophages and epithelial cells. To identify cytokine expression profiles, both the THP-1-derived macrophages and BEAS-2B epithelial cells were stimulated with dsRNA or SEB. A total of 21 cytokines were evaluated in the culture supernatants. We observed that stimulation with dsRNA induced cytokine production in both cell types. However, cytokine production was not induced in SEB-stimulated epithelial cells, compared to the macrophages. The synergistic effect of dsRNA and SEB was evaluated observing cytokine level and intracellular phospho-signaling. Fifteen different types were detected in high-dose dsRNA-stimulated epithelial cells, and 12 distinct types were detected in macrophages; those found in macrophages lacked interferon production compared to the epithelial cells. Notably, a synergistic effect of cytokine induction by co-stimulation of dsRNA and SEB was observed mainly in epithelial cells, via activation of most intracellular phosphor-signaling. However, macrophages only showed an accumulative effect. This study showed that the type and severity of cytokine productions from the epithelium or macrophages could be affected by different intensities and a combination of dsRNA and SEB. Further studies with this approach may improve our understanding of the development and exacerbation of airway inflammation and asthma.

• Biomedical Science Letters
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• v.20 no.2
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• pp.96-102
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• 2014

Staphylococcal enterotoxin B (SEB) is bacterial toxin that induces the activation of immune cells. Because the inhibition of pro-inflammatory effect of SEB can resolve the inflammation, I determined the influence of functional or structural change of SEB on immune cells. The post translational modification of protein occurs through carbamylation. Carbamylation can change the structure of proteins and can modify the biological activity of protein. In the present study, I investigated the effect of carbamylated SEB (CSEB) on the inflammatory response mediated by LPS in HL-60 cells. To determine the anti-inflammatory effect of CSEB, I produced carbamylated SEB using potassium cyanate (KCN) and then examined whether CSEB involved in cytokine releases and apoptosis of LPS-stimulated HL-60 cells. Although CSEB had not any effect on the LPS-stimulated HL-60 cells, the protein levels of IL-8, TNF-${\alpha}$ and IL-$1{\beta}$ were significantly decreased by CSEB without cytotoxicity. CSEB also blocked Akt and NF-${\kappa}B$ activation. These results indicate that the suppressive effect of CSEB in LPS-stimulated cytokine releases is occurred by inhibition of Akt and NF-${\kappa}B$ activity. Through further studies, CSEB may be used as anti-inflammatory molecule that makes the immune system more efficient.

• Proceedings of the Materials Research Society of Korea Conference
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• 1992.05b
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• pp.124-124
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• 1992

• Convergence Security Journal
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• v.16 no.5
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• pp.87-93
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• 2016

An Interest and development of an automation of the manufactory technology has been attempted actively in domestic and outside country. In particular, small and medium-sized manufactory are striving for the improvement of productivity through the automation of the plant. And manufactories to comply with the German's Industry 4.0 standard has attempted worldwide recently. But, This is not performed because a substantial investment-cost and development-cost and high efforts. Therefore, we attempted to design and mockup the SEB to enhance the productivity of the factory automation system. SEB, in conjunction with RFID and WiFi networks, expanding the communication range than the automation system of the existing plant, the transfer speed was also improved rapidly. And to be able to solve the existing factory automation system of vulnerability security of the problem, it can be solved by adding a section for user authentication.

• Microbiology and Biotechnology Letters
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• v.33 no.3
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• pp.207-214
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• 2005

To characterize the genotypic traits of clinical methicillin-resistant Staphyiococus aureus (MRSA) isolates (n=49), major virulence-associated genes were detected by using PCR-based methods. All the MRSA isolates possessed coagulase gene and showed four polymorphism types [500bp ($6{\%}$),580bp ($27{\%}$), 660bp ($65{\%}$) and 740bp ($2{\%}$)] due to variable numbers of tandem repeats present within the gene. The four or five different loci of hemolysin gene family were dominant in the MRSA isolates,25 of which($51{\%}$) possessed a combination of hla / hlb / hld/ hlg / hlg-2 genes as the most prevalent type. The prevalence of enterotoxin genes was varied among the MRSA isolates. sea and seb genes were detected from all the MRSA isolates. But sei, tsst-1, seg, sec, and seh genes were detected from 31 ($63{\%}$), 16 ($33{\%}$), 14 ($29{\%}$), 8 ($16{\%}$), and 5 ($10{\%}$) isolates, respectively. sed and sej genes were detected from 1 ($2{\%}$) isolate, respectively. see, eta, and etb genes were not detected at all. sea / seb genes were co-detected from 11 ($23{\%}$) isolates, sea / seb / sei genes from 9 ($19{\%}$) isolates, and sea / seb / seg / sei / tsst-1 genes from 5 ($10{\%}$) isolates. Other genes were co-detected with below $10{\%}$ frequencies.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.14 no.1
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• pp.245-251
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• 2014

Mix network plays a key role in electronic voting to preserve anonymity and lots of mixnet schemes have been proposed so far. However, they requires complex and costly zero-knowledge proofs to provide their correct mixing operations. In 2010, Seb$\acute{e}$ et al. proposed an efficient and lightweight mixnet scheme based on a cryptographic secure hash function instead of zero-knowledge proofs. In this paper, we present a more efficient and faster mixnet scheme than Seb$\acute{e}$ et al.'s scheme under the same assumption. Also, our scheme is secure.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.23 no.1
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• pp.43-49
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• 2011

For the improvement of water quality in a harbor, several studies have been carried out on SEB (Seawater Exchange Breakwater) in recent years, but a problem has been shown whereby the water on the inside area far from the SEB cannot be easily exchanged. In order to solve the problem of the SEB, the Manifold channel, a new concept of the SEB, is introduced in this paper. By using the manifold channel, it is possible to exchange the water of the inside area for seawater from the outside. Here, to assess the outflow gates of the manifold channel governing flow behavior, a virtual manifold channel controlled the location, width and direction of outflow gates applied to the Jumunjin fishery port, where the SEB has been established. In addition, the desirable flow pattern of the port by utilizing the two layer current model is identified, and five general cases of the manifold channel are described in this paper. The model is verified by comparing with observation of the SEB model, and the results are in general agreement. From the results of the manifold channel, in the case of the Jumunjin fishery port, the small circulation of counter clockwise is necessary for the water exchange on the inside area, but it should be controlled by the outflow gates for other areas. Using the two layer current model, the desirable flow pattern of the port can be predicted, and the water exchange for the upper and lower layer can be examined. For the practical use of the manifold channel, further studies on the manifold channel will be necessary, and it may then be used broadly for the design of breakwater in the future.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.39 no.9
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• pp.851-857
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• 2015

In this paper, we estimate the time-dependent C(t) integrals under elastic-plastic-creep conditions. Finite-element (FE) transient creep analyses have been performed for single-edge-notched-bend (SEB) specimens. We investigate the effect of the initial plasticity on the transient creep by systematically varying the magnitude of the initial step load. We consider both the same stress exponent and different stress exponents in the power-law creep and plasticity to elastic-plastic-creep behavior. To estimate the C(t) integrals, we compare the FE analysis results with those obtained using formulas. In this paper, we propose a modified equation to predict the C(t) integrals for the case of creep exponents that are different from the plastic exponent.

• Journal of Food Hygiene and Safety
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• v.18 no.2
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• pp.79-86
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• 2003

Most of food poisoning is frequently raised from mass catering. Especially, staphylococci takes the large part of pathogenic agents which are related to the hygienic condition. Among total 98 samples, four staphylococci were isolated from food service environment such as drinking water (A), hands (D), refrigerator and apron (E) of 5 elementary school (A, B, C, D, E) in Gyeongnam Province. These isolated strains are characterized as 1 MRCNS (Methicilline Resistant Coagulase Negative Staphylococcus aureus) and 3 MSCPS (Methicilline Sensitive Coagulase positive Staphylococcus aureus). Also, production of enterotoxin B (sob gene) were examined by PCR which has known as a big problem because of their temperature resistance. Hence, PCR was performed on isolated 4 staphylococci. The all 4 isolated Staphylococcus aureus have 477 bp of seb gene. Antibiotics susceptibility test was completed on PCR detected strains. All strains were fully resistance to ampicillin and penicillin. The drinking water of A place has resistance to oxacilline, therefore this strain turned out to be MRSA (Methicilline Resistant Staphylococcus Aureus).