• Transactions of the Korean Society of Automotive Engineers
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• v.7 no.7
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• pp.66-75
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• 1999

Many researchers have developed the measurement technique of in-cylinder flow. Recently, there are lots of studies on steady flow with different SCV geometries. In this study the flow characteristics of four-valve cylinder head were examined in a steady test rig for different SCV open ratios. Each swirl ratio of several SCV angle was quantified by swirl impulse meter and 2D-LDV measurement. The results showed that the swirl ratio was controlled between 2.3 and 3.8 based on SCV angles. The velocity distributions of in-cylinder flow field were measured by 2-D LDV test and visualized swirl and tumble flow pattern at different positions. In this engine , we found out that the swirl was dominant flow.

• Research in Plant Disease
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• v.26 no.3
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• pp.170-178
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• 2020

Most strawberry viruses exist relatively low titers in tissues, and strawberry tissues include high levels of contamination by polysaccharides and phenolic compounds. These traits make the efficiency of strawberry diagnosis difficult. In this study, we tested different commercially available kits and reagents to secure optimal RNA extraction methods to determine virus detection from strawberry leaves. Total RNA was isolated from leaves of strawberry mottle virus (SMoV)-infected strawberry cultivar 'Mihong'. The efficiency of total RNA for virus diagnosis was confirmed through SMoV detection by one-step or two-step reverse transcription and polymerase chain reaction (RT-PCR). Among those, the RNeasy plant RNA kit was best to isolate RNA and the isolated RNA was good enough for further applications. To ensure a reliable detection for strawberry viruses, synthetic diagnosis clones for major seven strawberry viruses such as strawberry mild yellow edge virus, SMoV, strawberry latent ring spot virus, strawberry crinkle virus, strawberry pallidosis associated virus, strawberry vein banding virus and strawberry necrotic spot virus have been constructed. Based on the synthetic genes in each clone, primer sets for seven strawberry viruses were designed and tested an RT-PCR condition through a simultaneous application of the same annealing temperature that allowed to achieve an efficient and convenient diagnosis.