• Title/Summary/Keyword: SAg

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Structure and Function of the Autolysin SagA in the Type IV Secretion System of Brucella abortus

  • Hyun, Yongseong;Baek, Yeongjin;Lee, Chanyoung;Ki, Nayeon;Ahn, Jinsook;Ryu, Sangryeol;Ha, Nam-Chul
    • Molecules and Cells
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    • v.44 no.7
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    • pp.517-528
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    • 2021
  • A recent genetic study with Brucella abortus revealed the secretion activator gene A (SagA) as an autolysin component creating pores in the peptidoglycan (PGN) layer for the type IV secretion system (T4SS) and peptidoglycan hydrolase inhibitor A (PhiA) as an inhibitor of SagA. In this study, we determined the crystal structures of both SagA and PhiA. Notably, the SagA structure contained a PGN fragment in a space between the N- and C-terminal domains, showing the substrate-dependent hinge motion of the domains. The purified SagA fully hydrolyzed the meso-diaminopimelic acid (DAP)-type PGN, showing a higher activity than hen egg-white lysozyme. The PhiA protein exhibiting tetrameric assembly failed to inhibit SagA activity in our experiments. Our findings provide implications for the molecular basis of the SagA-PhiA system of B. abortus. The development of inhibitors of SagA would further contribute to controlling brucellosis by attenuating the function of T4SS, the major virulence factor of Brucella.

Impact of cable sag on the efficiency of an inertial mass damper in controlling stay cable vibrations

  • Wang, Zhi-hao;Gao, Hui;Xu, Yan-wei;Chen, Zheng-qing;Wang, Hao
    • Smart Structures and Systems
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    • v.24 no.1
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    • pp.83-94
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    • 2019
  • Passive negative stiffness dampers (NSDs) that possess superior energy dissipation abilities, have been proved to be more efficient than commonly adopted passive viscous dampers in controlling stay cable vibrations. Recently, inertial mass dampers (IMDs) have attracted extensive attentions since their properties are similar to NSDs. It has been theoretically predicted that superior supplemental damping can be generated for a taut cable with an IMD. This paper aims to theoretically investigate the impact of the cable sag on the efficiency of an IMD in controlling stay cable vibrations, and experimentally validate superior vibration mitigation performance of the IMD. Both the numerical and asymptotic solutions were obtained for an inclined sag cable with an IMD installed close to the cable end. Based on the asymptotic solution, the cable attainable maximum modal damping ratio and the corresponding optimal damping coefficient of the IMD were derived for a given inertial mass. An electromagnetic IMD (EIMD) with adjustable inertial mass was developed to investigate the effects of inertial mass and cable sag on the vibration mitigation performance of two model cables with different sags through series of first modal free vibration tests. The results show that the sag generally reduces the attainable first modal damping ratio of the cable with a passive viscous damper, while tends to increase the cable maximum attainable modal damping ratio provided by the IMD. The cable sag also decreases the optimum damping coefficient of the IMD when the inertial mass is less than its optimal value. The theoretically predicted first modal damping ratio of the cable with an IMD, taking into account the sag generally, agrees well with that identified from experimental results, while it will be significantly overestimated with a taut-cable model, especially for the cable with large sag.

The Detection of Voltage Sag using Wavelet Transform (웨이브렛 변환을 이용한 Voltage Sag 검출)

  • Kim, Cheol-Hwan;Go, Yeong-Hun
    • The Transactions of the Korean Institute of Electrical Engineers A
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    • v.49 no.9
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    • pp.425-432
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    • 2000
  • Wavelet transform is a new method fro electric power quality analysis. Several types of mother wavelets are compared using voltage sag data. Investigations on the use of some mother wavelets, namely Daubechies, Symlets, Coiflets, Biorthogonal, are carried out. On the basis of extensive investigations, optimal mother wavelets for the detection of voltage sag are chosen. The recommended mother wavelet is 'Daubechies 4(db4)' wavelet. 'db4', the most commonly applied mother wavelet in the power quality analysis, can be used most properly in disturbance phenomena which occurs rapidly for a short time. This paper presents a discrete wavelet transform approach for determining the beginning time and end time of voltage sags. The technique is based on utilising the maximum value of d1(at scale 1) coefficients in multiresolution analysis(MRA) based on the discrete wavelet transform. The procedure is fully described, and the results are compared with other methods for determining voltage sag duration, such as the RMS voltage and STFT(Short-Time Fourier Transform) methods. As a result, the voltage sag detection using wavelet transform appears to be a reliable method for detecting and measuring voltage sags in power quality disturbance analysis.

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Evaluation of Aerodynamic Performance of Solar Wing System (솔라윙 시스템의 풍진동 특성 평가)

  • Kim, Yong Chul;Yoon, Sung-Won
    • Journal of Korean Association for Spatial Structures
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    • v.16 no.1
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    • pp.65-72
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    • 2016
  • Aerodynamic performance of solar wing system has been evaluated through wind tunnel test. The test model has 12 panels, each supported by 2 cables. The panels were installed horizontally flat, and gaps between panels were set constant. Sag ratios of 2% and 5%, and wind directions between $0^{\circ}$ and $90^{\circ}$ were considered. Mass of test model was determined considering the mass of full scale model, and Froude number and Elastic parameter were satisfied by adjusting the mean wind speed. From the wind tunnel test, it was found that the aerodynamic performance of the solar wing system is very dependent on the wind directions and sag ratios. When the sag was 2%, the fluctuating displacements between the wind directions of $0^{\circ}$ and $30^{\circ}$ increase proportionally to the square of the mean wind speed, implying buffeting-like vibration and a sudden increase in fluctuating displacement was found at large mean wind speed for the wind directions larger than $40^{\circ}$. When the wind direction was larger than $60^{\circ}$, a sudden increase was found both at low and large mean wind speed. When the sag ratio is 5%, distribution of mean displacements is different from that of sag ratio of 2%, and the fluctuating displacements show very different trend from that of sag ratio of 2%.

An Assessment Method for Voltage Sag in Power Distribution System using a Fuzzy Model (퍼지 모델을 이용한 배전 계통에서의 순간전압강하 평가 방식)

  • Yun, Sang-Yun;O, Jeong-Hwan;Kim, Eon-Seok;Kim, Nak-Gyeong;Kim, Jae-Cheol
    • The Transactions of the Korean Institute of Electrical Engineers A
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    • v.49 no.4
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    • pp.177-184
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    • 2000
  • In this paper, we proposes a method for assessing the effect of voltage sag in power distribution systems using fuzzy model. The proposed method is based on the reliability data of distribution system and specified computer business equipment manufacturer association(SCBEMA) curve that express the representative power acceptability curve by voltage sag for each customer type. The SCBEMA curves are made by using the CBEMA curves obtained from the experiment for the customers sensitive equipment. In order to transform SCBEMA curves to the differential damage by voltage sag, a fuzzy model is used. The proposed fuzzy model is composed to reflect two parameters of customers damage by voltage sag. One is the duration and magnitude of voltage sag and the other is the different risk due to the customer types. The Monte Carlo simulation method and the historical reliability data in KEPCO ae used for case studies.

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Development of Sag and Tension Sensitivity Estimation Method for Configuration Control under PPWS Erection in a Suspension Bridge (현수교 PPWS 가설중 형상관리를 위한 PPWS 새그 및 장력민감도 산정법 개발)

  • Jeong, Woon;Seo, Ju Won;Lee, Won Pyo
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.32 no.5A
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    • pp.255-266
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    • 2012
  • Main cable of a suspension bridge is the important member which shows the overall structure integrity at bridge completion. Configuration of main cable is a free hanging state at cable erection completion and is different from that at bridge completion supporting the dead loads such as hanger, girder, and so on. Accordingly, the configuration control under cable erection is considerably significant because the configuration at cable erection completion has direct influence on that at bridge completion. That is performed by sag adjustments at center, side span and tension adjustments at anchor span. The former needs the sag sensitivity which represents the control quantity of strand length corresponding to that of sag. The latter requires the tension sensitivity which shows the change of strand tension according to that of strand temperature. In this study, the fundamental equations of cable were derived with the assumption of either catenary or parabola shape, the differential-related equations using chain rule on horizontal tension were drawn from those and finally the estimation methods of the sag / tension sensitivity were proposed from both those. The nonlinear numerical analysis flow charts of sag sensitivity based on the catenary equations were proposed and the sag sensitivities grounded on the differential-related equations were compared with the results using them for various parameters of sag change. Also, considering the combinations of sag change parameters, the calculation method of the final variation for the cable sag was suggested. For the real suspension bridge under construction with PPWS method, the sag/tension sensitivity were estimated considering the construction conditions like the change of PPWS length, PPWS temperature, bridge span, etc.. We hope that this study will be a systematic guideline for the configuration control under main cable erection and improved highly by field verification in the real bridge site.

Response to Plant Hormones of Senescence-related Genes for Cucumis sativus L. in Cotyledon Development (오이 떡잎에서 노쇠화 관련 유전자들의 식물 호르몬에 대한 반응 연구)

  • Cha, Hyeon Jeong;Kim, Dae-Jae
    • Journal of Life Science
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    • v.26 no.8
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    • pp.895-903
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    • 2016
  • This study was carried out to discover the response of cucumber (Cucumis sativus L.) senescence- associated genes (SAGs) to several plant hormones in detached and developing cotyledon. Accordingly, a collection of cucumber SAGs were examined to characterize their gene expression response through semi-quantitative RT-PCR. Cotyledons were excised at day 14 after seed sowing from plantlets, then incubated in 100 μM each of IAA or zeatin solution for up to 4 days in light and darkness. They were collected at 2-day intervals and used for total RNA extraction and subjected to RT-PCR. Gene expression levels of several cucumber SAGs were significantly changed during the incubation period. More than five cucumber SAGs involving SAG 60 responded to the IAA and zeatin treatment. In the ethylene response study, cotyledons were exposed up to 10 days by ethylene gas. Most of the cucumber SAGs did not show immediate response to ethylene in green cotyledon. The exceptions were PCK, SAG 158, and SAG 288 genes, which responded after 1 day of exposure to green cotyledon, while ICL and SAG 281 revealed strong responses after 10 days of being exposed to yellowing cotyledon. These results suggest that several cucumber SAGs react actively in response to starvation or senescence against exogenously applied stimulus. This induced senescence response is able to understand the SAGs role in lipids and amino acids metabolism partly and function in organ senescence during development.

Z-Source Voltage Sag Compensator (Z-소스 전압Sag 보상기)

  • Kim, J.H.;Oum, J.H.;Jung, Y.G.;Lim, T.C.
    • Proceedings of the KIPE Conference
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    • 2007.07a
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    • pp.485-487
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    • 2007
  • 본 연구에서는 Z-소스 네트워크를 갖는 순시전압sag 보상기를 제안하였다. 제안된 시스템은 연료전지 스택(fuel cells stack)을 갖고 있는 Z-소스 인버터의 shoot-through제어에 의하여 ac보상전압을 직접 발생한다. PSIM 시뮬레이션에 의하여 sag와 swell이 발생되는 정상상태와 과도상태에서의 제안된 방법의 타당성을 검토하였다.

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Characteristic Analysis of Voltage Sags Due to Faulted Distribution Lines (배전선로 고장에 의한 Voltage Sag의 특성 해석)

  • ;Madhat M. Morcos
    • Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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    • v.16 no.1
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    • pp.76-84
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    • 2002
  • Voltage sags caused by line faults in transmission and distribution lines have become one of the most important power quality problems facing industrial customers and utilities. Voltage sags are normally described by characteristics of both magnitude and duration, but phase angle shifts should be taken account in identifying sag phenomena and finding their solutions. In this paper, voltage sags due to line faults such as three phase-to-ground, single line-to-ground, and line-to-line faults are characterized by using symmetrical component analysis, for fault impedance variations. Voltage sags and their effect on the magnitude and phase angle are examined. Balanced sags of three phase-to-ground faults show that voltages and currents are changed with equivalent levels to all phases and the zero sequence components become zero. However, for unbalanced faults such as single line-to-ground and line-to-line faults, voltage sags give different magnitude variations and phase angle shifts for each phase. In order to verify the analyzed results, some simulations based on power circuit models are also discussed.

Fast Detection Algorithm for Voltage Sags and Swells Based on Delta Square Operation for a Single-Phase Inverter System

  • Lee, Woo-Cheol;Sung, Kook-Nam;Lee, Taeck-Kie
    • Journal of Electrical Engineering and Technology
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    • v.11 no.1
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    • pp.157-166
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    • 2016
  • In this paper, a new sag and peak voltage detector is proposed for a single-phase inverter using delta square operation. The conventional sag detector is from a single-phase digital phase-locked loop (DPLL) that is based on d-q transformations using an all-pass filter (APF). The d-q transformation is typically used in the three-phase coordinate system. The APF generates a virtual q-axis voltage component with a 90° phase delay, but this virtual phase cannot reflect a sudden change in the grid voltage at the instant the voltage sag occurs. As a result, the peak value is drastically distorted, and it settles down slowly. A modified APF generates the virtual q-axis voltage component from the difference between the current and the previous values of the d-axis voltage component in the stationary reference frame. However, the modified APF cannot detect the voltage sag and peak value when the sag occurs around the zero crossing points such as 0° and 180°, because the difference voltage is not sufficient to detect the voltage sag. The proposed algorithm detects the sag voltage through all regions including the zero crossing voltage. Moreover, the exact voltage drop can be acquired by calculating the q-axis component that is proportional to the d-axis component. To verify the feasibility of the proposed system, the conventional and proposed methods are compared using simulations and experimental results.