• 생명과학회지
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• 제19권7호
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• pp.845-851
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• 2009

본 연구는 5-L 발효기를 이용하여 재조합 고스트 박테리아(E.coli $DH5{\alpha}$/ pHCE-InaN-(enolase, GAPDH, sagA or piaA)-ghost 37 SDM) 백신의 산업화를 위해 탄소원 공급조건, 교반속도, 산소공급 조건등의 최적 배양조건과 고스트 박테리아 발현 유도를 위한 온도조절 시점과 그에 따른 발현효율 최적화를 조사하기 위해 수행하였다. 각각 다른 4종의 항원 유전자를 보유한 고스트 박테리아를 LB 배지를 이용하여 배양한 결과 모두 1 g / 1 glucose, 300 rpm, 1vvm에서 최대 균주 성장을 나타내었다. 고스트 박테리아 생성 효율의 경우 초기 대수증식기(OD$_{600}$=1.0)에서 고스트 발현을 유도했을 때 각각 최대효율인 99.99%를 나타내었으나 증기 대수증식기(OD$_{600}$=2.0)와 말기 대수증식기 (OD$_{600}$=3.0)에서는 고스트 박테리아 생성이 낮은 효율을 나타내었다. 또한 SDS-PAGE 와 western blot를 이용하여 각각 다른 4종의 항원 단백질 발현 여주를 확인한 결과 enolase (78kda), GAPDH (67kda),sagA(26kDa), piaA(26kDa)에서 항원 단백질 band를 확인할 수 있었다. 따라서 본 연구결과 확립된 배양 조건과 발현효율 최적화 조건은 연쇄구균증 질병에 대해 E.coli를 이용한 고스트 박테리아 백신이 양식 산업에 있어 상업적으로 유용한 백신의 최적생산을 위해 사용 될 수 있을 것으로 사료된다.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2011년도 추계학술대회 논문집
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• pp.432-433
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• 2011

• Journal of Electrical Engineering and Technology
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• 제12권2호
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• pp.569-575
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• 2017

This paper proposes a method to utilize an energy storage system (ESS) based on the assessment of an area of severity (AOS) to voltage sag. The AOS is defined as a set of the fault positions that can cause voltage sags at many buses simultaneously. The assessment of AOS helps to determine an optimal location of ESS installation to minimize the expected sag frequency (ESF) at concerned buses. The ESS has the ability not only to play traditionally known roles but also to mitigate voltage sag impact on renewable energy sources (RES) in the islanded microgrid. Accordingly, using the proposed method the ESS has additional features to prevent the operation failure of RESs and improve the stability of the microgrid. In order to verify the presented method, a case study was conducted on the sample microgrid system that is modified from an IEEE 57-bus system.

• 한국전기전자재료학회논문지
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• 제24권5호
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• pp.410-415
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• 2011

In this paper, we investigated the fault current limiting and the load voltage sag suppressing characteristics of the flux-lock type SFCL, designed with the additive polarity winding, according to the variations of turn number's ratio and the comparative analysis between the resistive type and the flux-lock type SFCLs were performed as well. From the analysis for the short-circuit tests, the flux-lock type SFCL designed with the larger turn number's ratio was shown to perform more effective fault current limiting and load voltage sag suppressing operations compared to the flux-lock type SFCL designed with the lower turn number's ratio through the fast quench occurrence of the high-$T_C$ superconducting (HTSC) element comprising the flux-lock type SFCL. In addition, the recovery time of the flux-lock type SFCL after the fault removed could be confirmed to be shorter in case of the flux-lock type SFCL designed with the lower turn number ratio.

• 한국전기전자재료학회논문지
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• 제24권2호
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• pp.141-146
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• 2011

When the current of the superconducting element exceeds its critical current by the fault occurrence, the quench of the high-$T_C$ superconducting fault current limiter (HTSC) comprising the flux-lock type superconducting fault current limiter (SFCL) occurs. Simultaneously, the magnetic flux in the iron core induces the voltage in each coil, which contributes to limit the fault current. In this paper, the fault current limiting characteristics of the flux-lock type SFCL as well as the load voltage sag suppressing characteristics according to the flux-lock type SFCL's winding direction were investigated. To confirm the fault current limiting and the voltage sag suppressing characteristics of the this SFCL, the short-circuit tests for the simulated power system with the flux-lock type SFCL were carried out. The flux-lock type SFCL designed with the additive polarity winding was shown to perform more effective fault current limiting and load voltage sag suppressing operations through the fast quench occurrence right after the fault occurs and the fast recovery operation after the fault removes than the flux-lock type SFCL designed with the subtractive polarity winding.

• 조명전기설비학회논문지
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• 제23권10호
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• pp.21-28
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• 2009

전력용 커패시터는 유도성 부하의 갖은 역율을 보상하기 위해 사용되지만, 전력변환장치 등에 의해 발생하는 고조파를 저감하기 위해 리액터와 함께 사용하기도 한다. 전기품질은 대부분이 전압품질에 관련된 것으로서 부하의 안정적인 동작에 매우 중요하지만, 순간적인 전압강하 또는 순간 전압 융기 발생시와 같이 전압의 크기가 일시적으로 변할 경우 커패시터에 전기적인 스트레스로 작용할 수 있다. 본 연구에서는 순간적인 전압강하 및 순간 전압 융기 발생시 커패시터에서의 전압, 전류 및 용량의 변화를 해석하였으며, 커패시터에 리액터를 설치할 경우 순간적인 전압 강하시는 문제가 되지 않지만, 순간 전압 융기 발생 영역에서는 커패시터에 상당한 스트레스를 줄 수 있다는 것을 확인할 수 있었다.

• Structural Engineering and Mechanics
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• 제74권4호
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• pp.533-545
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• 2020

An optimal design method for a spoke double-layer cable-net structure (SDLC) is proposed in this study. Simplified calculation models of the SDLC are put forward to reveal the static responses under vertical loads and wind loads. Next, based on an energy principle, the relationship among the initial prestress level, cross-sectional areas of the components, rise height, sag height, overall displacement, and relative deformation is proposed. Moreover, a calculation model of the Foshan Center SDLC is built and optimized. Given the limited loading cases, material properties of the components, and variation ranges of the rise height and sag height, the self-weight and initial prestress level of the entire structure can be obtained. Because the self-weight of the cables decreases with increasing of the rise height and sag height, while the self-weight of the inner strut increases, the total weight of the entire structure successively exhibits a sharp reduction, a gradual decrease, a slow increase, and a sharp increase during the optimization process. For the simplified model, the optimal design corresponds to the combination of rise height and sag height that results in an appropriate prestress level of the entire structure with the minimum total weight.

• Structural Engineering and Mechanics
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• 제20권3호
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• pp.363-380
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• 2005

The cable tension plays an important role in the construction, assessment and long-term health monitoring of cable structures. The cable vibration equation is nonlinear if cable sag and bending stiffness are included. The engineering implementation of a vibration-based cable tension evaluation is mostly carried out by the simple taut string theory. However, the simple theory may cause unacceptable errors in many applications since the cable sag and bending stiffness are ignored. From the practical point of view, it is necessary to have empirical formulas if they are simple and yet accurate. Based on the solutions by means of energy method and fitting the exact solutions of cable vibration equations where the cable sag and bending stiffness are respectively taken into account, the empirical formulas are proposed in the paper to estimate cable tension based on the cable fundamental frequency only. The applicability of the proposed formulas is verified by comparing the results with those reported in the literatures and with the experimental results carried out on the stay cables in the laboratory. The proposed formulas are straightforward and they are convenient for practical engineers to fast estimate the cable tension by the cable fundamental frequency.

• Parasites, Hosts and Diseases
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• 제41권3호
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• pp.175-177
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• 2003

An ELISA was established to measure bovine IgG directed against the recombinant antigenic determinant of Nc-p43, a major surface antigen of Neospora caninum. In a previous study, two thirds of the C-terminal of the molecule was expressed as a $6{\;}{\times}{\;}His$ tagged protein (Ncp43p) for ELISA using 2/3 of the N-terminal of SAG1 from Toxoplasma gondii as a control (TgSAG1A). Among 852 cattle sera collected from stock farms scattered nation-wide, 103 sera (12.1％) were found to react with Ncp43p positively, but no positive reaction was observed with TgSAG1A. This study shows that Ncp43p could be available as an efficient antigen for the diagnosis of neosporosis in cattle. Furthermore, it together with TgSAG1A, could be useful for the differential diagnosis of N. caninum and T.gondii infections in other mammals.

• 조명전기설비학회논문지
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• 제21권4호
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• pp.36-44
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• 2007

This study researched the mutual relationship between elevator emergencies(sudden stops, entrapment) and power quality such as voltage interruptions or sags. To analyze the power quality at the elevator switchboard of an apartment, the on-line power quality(sag, swell, and interruption, etc.) were measured and error messages(under voltage error, etc.) in the elevator control room were researched. Also the performance and susceptibility for stops were evaluated and stated through the testing of three pieces of simulated test equipment with an EN12016(2004) standard setting and the magnitude and duration data of the measured sag or interruption. This paper will use these data in the analysis of the mutual relationship between power quality and elevator emergencies.