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Comparison of Molecular Characteristics of Extended Spectrum ${\beta}$-lactamase Producing Escherichia coli Strains Isolated from Patients with Urinary Tract Infections between 2 Time Periods of 1989 and 2010 at Gangwon Province in Korea

  • Park, Min;Park, Soon Deok;Kim, Sa-Hyun;Lee, Gyusang;Woo, Hyun Jun;Kim, Hyun Woo;An, Byungrak;Jang, In Ho;Uh, Young;Kim, Jong-Bae
    • Biomedical Science Letters
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    • v.19 no.3
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    • pp.275-279
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    • 2013
  • Etiological agents of extended spectrum ${\beta}$-lactamase (ESBL) producing uropathogenic Escherichia coli (UPEC) have become a major problem in urinary tract infections. The purpose of this study was to compare the molecular characteristics of ESBL producing UPEC strains isolated from 1989 and 2010. A total of 301 strains of UPEC clinical isolates was collected from Korean healthcare facility in 1989 (126 strains) and in 2010 (175 strains). UPEC clinical isolates were analyzed by multiplex polymerase chain reaction method (ESBL related bla genes and phylogenetic groups) and amplified fragment length polymorphism (AFLP). Among 301 isolates, ESBL producing UPEC were 8 strains (6.3%) in 1989 isolates and 35 strains (20%) in 2010 isolates. The rate of bla genes in ESBL producing UPEC from 1989 isolates and 2010 isolates were $bla_{TEM}$ (75% and 85.7%), $bla_{CTX-M}$ (0% and 91.4%), $bla_{OXA}$ (25% and 20%), $bla_{PER}$ (0% and 2.9%). The distribution of phylogenetic groups in 1989 isolates and 2010 isolates were A (37.5% and 11.4%), B2 (12.5% and 51.4%), and D (50% and 37.1%). The most prevalent ESBL related bla gene and phylogenetic group were $bla_{CTX-M}$ (91.4%) and B2 (51.4%) in 2010 isolates, while $bla_{CTX-M}$ was not detected in 1989 isolates. Among 43 ESBL producing UPEC were grouped into 12 clusters up to 76% of genetic similarities by AFLP analysis. During past twenty one years, the rate of the ESBL producing UPEC strains in 2010 isolates was increased than that of in 1989 isolates. Also, the most prevalent ESBL related bla gene has been changed from $bla_{TEM}$ to $bla_{CTX-M}$.

Catechin Content and Antioxidative Effect from Rosa davurica Pall (생열귀나무의 카테킨 함량 및 항산화효과)

  • Shin, In-Cheol;Jeong, Kyung-Jin;Shim, Tae-Heum;Oh, Heung-Seok;Park, Sang-Kyun;Cheung, Eui-Ho;Kim, Suk-Nam;Kim, Gwang-Gee;Choi, Dae-Sung;Kwon, Yong-Soo;Kim, Chang-Min;Sa, Jae-Hoon
    • Korean Journal of Pharmacognosy
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    • v.33 no.3 s.130
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    • pp.177-181
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    • 2002
  • This study was carried out to investigate the antioxidative activities of Rosa davurica Pall for the purpose of development of novel antioxidant from natural products. Antioxidant activities of four different parts of Rosa davurica Pall such as fruit, leaf, stem and root were examined by measuring the radical scavenging effect on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical. The methanol extract from the root of Rosa davurica Pall showed the highest antioxidative activity among 16 samples tested. And, we also tested radical scavenging effects of 5 different extract compartments(Hexane, $CHCl_3$, EtOAc, BuOH and $H_2O$ fraction). EtOAc and BuOH fractions from the root of Rosa davurica Pall exhibited antioxidative activities higher to those of natural, ${\alpha}-tocopherol$ or synthetic antioxidants, BHT. The antioxidative substance of EtOAc fraction from the root of Rosa davurica Pall was successively purified with silica gel adsorption column chromatography and Sephadex LH-20 column chromatography. The purified active substance was isolated as crystal and identified as (+)-catechin by $^{l}H-NMR$ and $^{13}C-NMR$. This compound exhibited DPPH radical scavenging activity with the $IC_50$ value of $1.7\;{\mu}g/ml$. In the analysis of catechin content, the leaf extracts contained the highest catechin, and fruit extracts contained the lowest catechin. Considering antioxidative activity on DPPH assay, the extracts of Rosa davurica Pall showed a possibility to be used as a new material for natural antioxidant and functional food.

Usefulness of Partial Muscle Flaps and Combination Method for Coverage of Prosthetic Material in Chest Wall Reconstruction (흉벽 재건에서 합성 물질의 피복을 위한 부분 근피판 조합의 효용성)

  • Ryoo, Suk-Tae;Pyon, Jai-Kyong;Lim, So-Young;Mun, Goo-Hyun;Bang, Sa-Ik;Oh, Kap-Sung
    • Archives of Plastic Surgery
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    • v.38 no.3
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    • pp.228-234
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    • 2011
  • Purpose: Reconstruction of chest wall has always been a challenging problem. Muscle flaps for chest wall reconstruction have been helpful in controling infection, filling dead space and covering the prosthetic material in this challenge. However, when we use muscle flaps, functional and cosmetic donor site morbidities could occur. The authors applied and revised various partial muscle flaps and combination use of them to cover the prosthetic material for the chest wall reconstruction and evaluated the usefulness of partial muscle flaps. Methods: This study included 7 patients who underwent chest wall reconstruction using partial muscle flap to cover prosthetic material from 2004 to 2008. The pectoralis major muscle was used in anterior 2/3 parts of it leaving lateral 1/3 parts of it. The anterior 2/3 parts of the pectoralis major muscle were used while lateral 1/3 parts were left. In case of the rectus abdominis muscle flap, we used upper half of it, or we dissected it around its origin and then advanced to cover the site. The latissimus dorsi muscle flap was elevated with lateral portion of it along the descending branch of the thoracodorsal artery. If single partial muscle flap could not cover whole prosthetic material, it would be covered with combination of various partial muscle flaps adjacent to the coverage site. Results: Flap coverage of the prosthetic material and chest wall reconstructions were successfully done. There occurred no immediate and delayed post operative complications such as surgical site infection, seroma, deformity of donor site and functional impairment. Conclusion: When we use the muscle flaps to cover prosthetic material for chest wall reconstruction, use of the partial muscle flaps could be a good way to reduce donor site morbidity. Combination of multiple partial flaps could be a valuable and good alternative way to overcome the disadvantages of partial muscle flaps such as limitation of volume and size as well as flap mobility.

Factors Affecting Smoking Attitude and Intention among Preschool Children (유치원 아동의 흡연태도 및 흡연의도에 미치는 영향요인)

  • Lee Hung Sa;Paik Sun-bok;Kim Hyun Chull;Choi Gyoung Hee
    • Journal of Korean Public Health Nursing
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    • v.18 no.2
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    • pp.330-341
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    • 2004
  • Parental smoking status and smoking environment do not only affect children's health through exposure to nicotine, but are also related to children's smoking attitude, intention and knowledge of smoking. Since this study was conducted to examine a relationship between family members smoking and smoking attitude, intention and knowledge of their children, little is known about psychosocial factors influencing smoking among Korean preschool children. This study aimed to determine the psychosocial factors (sociodemographic factors, education for smoking cigarette, knowledge about smoke cigarette) influencing smoking attitude and smoking intention, with a purpose of providing directions for future education programs for Korean preschool children. A cross sectional sample of 840 children, ages 4-7 years, living in a rural district were interviewed during August 6 - November 14, 2003. Among them, 834 children whose missing data were less than $10\%$ were included the analysis. The independent variables included sociodemographic characteristics and education for smoking cigarette, while the dependent variables were knowledge about smoking, the children's smoking attitude and their intention of smoking. The data on sociodemographic characteristics were analyzed using descriptive statistics including frequency, percentage, mean, and standard deviation. The correlations between the independent variables and attitude and intention of smoking were analyzed using chi-square test, t-test, and logistic regression. The results of this study were s follows. The factors that were found to be significantly correlated to smoking attitude include mother's smoking $(x^2=4.86,\;p=.03)$, grandmother's smoking $(x^2=5.56,\;p=.02)$, and knowledge of smoking cigarette $(x^2=-5.13 p<.01)$. The factors that were significantly correlated to the intention of smoking include gender $(x^2=.08,\; p=.02)$, education for smoking$(x^2=17.65,\;p<.01)$, mother's smoking $(x^2=19.05,\; p<.01)$, grandmother's smoking $(x^2=17.67,\; p<.01)$, and knowledge$(x^2=-2.17,\;p=.03)$. The children who had non smoking mother were more likely to have negative attitude (OR=2.29). The children who had a higher knowledge about smoking were more likely to have negative attitude (OR=1.58). The findings suggest that a smoking prevention program specific for preschool children is necessary to prevent early onset of smoking. The findings also suggest that the program should involve children's family members who influence their smoking attitude and intention.

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A Study on the Historic Changes of Yungnam-Ru in Historic Periods and Architectural Building Forms (밀양 영남루 연혁 및 건축형식 변천에 관한 연구)

  • Lee, Ho-Yeol
    • Journal of architectural history
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    • v.9 no.1 s.22
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    • pp.7-25
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    • 2000
  • The purpose of this study is focused to the historic changes of Yungnam-Ru(嶺南樓), which are based on the site layout, and architectural forms in architectural building history and historic periods analyzed with the historic reference, paintings, and photos. This study is to search the alteration of the types of axis and the formation of spaces in Yungnam-Ru which is the Ru-Gak(樓閣) The conclusion of the architectural changes from the historic period and architectural form which is the belows. The first Yungnam-Ru by name had been used to be called, before it was re-called Yungnam-Ru by Kim Ju in 1365. Therefore, the hypothesis in naming Yungnam-Ru form assumption that the building under the name of Yungnam-Ru was re-named by Kim Ju from the Old Budist Temple called Yungnam-Sa, should re-considered in history. The second, it is considered that Milyang-Sibyi-Kyungdo(密陽十二景圖) as the painting can only be seen the site layout in 1542. It could be compared the differences of the site layouts from in 1542 to the present time. At that time Nyungpadang(凌派堂) was connected the main buildings called Yungnam-Ru Chimrudang(沈流堂),building was seperated with the Yungnam-Ru at that period. In 1542, the main Building(Yungnam-Ru) was consist of 5 spans of columns(from the front) and 2 spans of columns(from the side). Now, the main Building(Yungnam-Ru) has the 5 spans of columns from the front, and two spans of columns short from the side, compared to the present facade. At the past, Chimrudang(building) has the two spans of columns and one span of columns short, compared to the present facade. The third, It supposed that main building, Nyungpadang and Chimrudang in the composite of facade was connected with Wolrang(月廊) and Hunrang(軒廊) after invatioin from japan in 1592. 1844, (Chosun dynasty, Hunjong 10) the Yungnam-Ru was re-builted by maintaining the same concept in site layout of the past, and finally the three buildings was put together with Wolrang and Hunrang. As a result, the plan of the Yungnam-Ru was expanded with many aspects. From 1542 to 1844, the present site-layout gradually completed with three buildings which was spatially connected. The forth, in the middle age of Chosun dynasty, after added Gaeksa(客舍), the building is for the government officer staying temporally from outside province) in the site, the site layout was greatly changed with volume of building. In 1844, the Yungnam-Ru as the Nugak belongs to Miljukwan(密州館) was expanded spacially and formally. After that time, the burned buildings could not have been re-built because of aspects in government ability and economical ability.

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Detection of Superior Markers for Polymerase Chain Reaction Diagnosis of Breast Cancer Micrometastasis in Sentinel Lymph Nodes

  • Shargh, Shohreh Alizadeh;Movafagh, Abolfazl;Zarghami, Nosratolah;Sayad, Arezou;Mansouri, Neda;Taheri, Mohammad;Pour, Atefeh Heidary;Iranpour, Mostafa;Ghaedi, Hamid;Montazeri, Vahid;Massoudi, Nilofar;Hashemi, Mehrdad;Mortazavi-Tabatabaei, SA
    • Asian Pacific Journal of Cancer Prevention
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    • v.17 no.sup3
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    • pp.179-183
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    • 2016
  • Breast cancer is the most prevalent type of cancer among women around the world, and mortality is primarily caused by micro-metastatic disease. The complex mechanisms of breast cancer invasion and metastasis are intrinsically related to the malignant cell type so that early detection of micro-metastases can help prolongation of survival for patient. The aim of the present research work was evaluation of the expression status of mammoglobin protein as a candidate molecular marker in the negative sentinel lymph node (SLN). Fifty tumor specimens, and 50 normal adjacent breast tissue samples from the same patients were selected on the basis of having more than 10% tumor content for RNA extraction from SLNs. Tumor samples and normal adjacent breast tissue were archived in the form of frozen fresh tissue in liquid nitrogen. Real-time PCR was performed on a Bioner life express gradient thermal cycler system. Mammoglobin gene overexpression in breast cancer metastasis was investigated. Single marker results were mammaglobin 66.7% and CK19 50.0%, with 58.3% for the two in combination. Due to improved outcome with at least 3 genes (83.3%), it seems, triple marker evaluation will be most likely useful for detecting micro-metastases instead of studying separate genes.

Life Style and Perception of Obesity of Male White Collar Workers (남성 사무직 근로자의 생활습관과 비만에 대한 인식)

  • Kim, Ji-Hyeon;SaKong, Joon
    • Journal of Yeungnam Medical Science
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    • v.24 no.2
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    • pp.287-295
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    • 2007
  • Background : This study investigated and analyzed the life style of male white- collar workers and their perception of obesity to determine how to improve eating habits and prevent obesity. Materials and Methods : Using the questionnaires distributed to and collected from 300 male white-collar workers in Daegu, the survey was conducted from December 20, 2005 to February 28, 2006. Two hundred sets of collected questionnaires were used for the analysis, and SPSS WIN 12.0 was employed to analyze the data. Results : Forty-two percent of subjects had weights ranging between 71 kg and 80 kg; 39.5%, weighted between 61 kg and 70 kg. The waist measurements of 64.5% of all respondents were between 32 and 34 inches. The lifestyle questions found that 54% of respondents were smokers and 88.5% drank alcohol. In addition, 62.5% of all respondents reported doing exercise, whereas 37.5% reported no exercise. Conclusion : The height and weight of 200 respondents were used to calculate the body mass index (BMI). Only 31.6% had a normal BMI, whereas 32% were found to be overweight, a condition likely to lead to obesity. Meanwhile, 36.5% of respondents were obese or morbidly obese.

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Quality of Bastard Halibut with Different Weights as a Surimi Source (연육 (surimi) 소재로서 어체중량이 다른 넙치의 품질 특성 비교)

  • Heu, Min-Soo;Shin, Jun-Ho;Park, Kwon-Hyun;Lee, Ji-Sun;Noe, Yu-Ni;Jeon, You-Jin;Kim, Jin-Soo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.44 no.1
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    • pp.18-24
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    • 2011
  • This study investigated the physicochemical and enzymatic properties of unmarketable cultured bastard halibut (Paralichthys olivaceus) of different weights as a potential source of surimi and surimi gel. The proximate composition of cultured bastard halibut of different weights did not differ significantly at P<0.05 (light weight (LBH) 400~500 g, medium weight (MBH) 600~800 g, and heavy weight (HBH) > 1,000 g). Compared to Alaska pollock muscle, the bastard halibut muscle had a 4% higher crude protein content and 6% lower moisture content. The collagen content of LBH bastard halibut muscle was 1.58 g/100 g, which was lower than or no different from bastard halibut weighing different amounts. Regardless of fish weight or pH, the enzymatic activities of crude fish extracts ranged from 0.34~0.48 U/mg for casein and hemoglobin, 11.0~12.7 U/mg for LeuPNA, 5.4~6.1 U/mg for ArgPNA, 2.3~2.9 U/mg for SAAPFNA, and 0.1~0.2 U/mg for BAPNA. The yield of surimi gel from LBH was 24.4%, which was similar to that from MBH and lower than that from HBH. The surimi gel from LBH was similar to that from HBH, while weaker than that from MBH. The surimi gel from LBH gel was stronger than grade SA gel from commercial Alaska pollock.

Design and Implementation of Transport and Session Managers in a Multimedia Streaming Framework (멀티미디어 스트리밍 프레임워크에서 전송 및 세션 관리자의 설계 및 구현)

  • Lim, Eak-Jin;Lee, Sung-Young;Jeong, Chang-Yun
    • Journal of KIISE:Computing Practices and Letters
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    • v.7 no.1
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    • pp.24-37
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    • 2001
  • This paper deals with our experience for the design and implementation of Transport and Session managers in the Integrated Streaming Service Architecture (IS SA) that was developed by the authors. The ISSA is a streaming framework that allows to develop integrated multimedia streaming applications such as VOD system in unicast/multicast and real-time broadcastOive-cast). It also facilities standard real-time transport protocols such as RTp(Real-Time Transport Protocol)/RTCP(Real-time Control Protocol) and RTSP(Real-Time Streaming ProtocoD that allows to user openness. The ISSA supports diverse media fonnats and is independent from underlying networks and operating systems, and compatible with the global real-time multimedia database system (BeeHive) so that streaming media are efficiently retrieved, stored, and serviced. The role of the Transport Manager is to do packetization and depacketization for the different types of multimedia data and delivers the packetized media data in real-time. The Transport Manager uses RTP protocol. The role of the Session Manager is to establish and control the media channel by using RTSP protocol and to deliver the database transactions for the multimedia database by using RTTP(Real-Time Transaction Protocol) protocol. Both the Transport and Session Manager are doing their functions through the network interface in the ISSA that allows developers to various network programming interfaces and provides flexibility to the system.

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In Vitro Assessment of MRI Safety at 1.5 T and 3.0 T for Bone-Anchored Hearing Aid Implant (Bone-Anchored Hearing Aid Implant에 대한 1.5 T와 3.0 T에서 MRI 안전성의 생체외 평가)

  • Yeon, Kyoo-Jin;Kim, Hyun-Soo;Lee, Seung-keun;Lee, Tae-Soo
    • Journal of radiological science and technology
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    • v.40 no.1
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    • pp.19-25
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    • 2017
  • The aim of this study was to evaluate Magnetic Resonance Imaging safety by measuring the translational attraction, torque and susceptibility artifact for Bone-Anchored Hearing Aid (BAHA) implant at 1.5 T and 3.0 T MRI by standard criteria. In vitro assessment tools were made of acrylic-resin by American Society for Testing and Materials (ASTM) F2052-06 and F2119-07 standard. Translational attraction of BAHA implant was measured by the maximum deflection angle at 96 cm position, where the magnetically induced deflection was the greatest. The torque was assessed by the qualitative criteria of evaluating the alignment and rotation pattern, when the BAHA implant was positioned on a line with $45^{\circ}$ intervals inside the circular container in the center of the bore. The susceptibility artifact images were obtained using the hanged test tool, which was filled with $CuSO_4$ solution. And then the artifact size was measured using Susceptibility A rtifact Measurement (SA M) software. In results, the translational attraction was 0 mm at both 1.5 T and 3.0 T and the torque was 0(no torque) at 1.5 T, and +1(mild torque) at 3.0 T. The size of susceptibility artifacts was between 13.20 mm and 38.91 mm. Therefore, The BAHA implant was safe for the patient in clinical MR environment.