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Comparison of the non-invasive diagnostic methods, stool antigen test and PCR assay, for Helicobacter felis detection in dogs

  • Hong, Sunhwa;Lee, Hak-Yong;Kim, Tae-Wan;Kim, Okjin
    • Korean Journal of Veterinary Service
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    • v.38 no.1
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    • pp.37-42
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    • 2015
  • The aim of the present study was to compare the non-invasive methods for the diagnosis of H. felis with HpSA kit-based detection method and H. felis-specific PCR assay with dog's stool samples without sacrifice. Male Beagle dogs (n=6) were infected with H. felis ATCC 49179 ($1.0{\times}10^9CFU/dog$) by intra-gastric inoculation two times at 3-day intervals, and the stool specimens of dogs were collected 1, 3, 5, 7, 14, 21 days after infection to submit to HpSA test and H. felis-specific PCR. As the results, the sensitivity of the HpSA and the PCR analysis was 50.0%, 83.3% respectively. Although HpSA test is less sensitive, it could be used for rapid, cheap and easy screening assay for H. felis infection in dog and cats. We suggest that the H. pylori stool antigen kit, HpSA, is useful and effective for monitoring H. felis infection. If HpSA test would be made with H. felis antibodies in the future, its sensitivity could be increased. Also, PCR assay could be successfully used to detect the H. felis in stools. Applying the H. pylori stool antigen kit and PCR assay may be the recommended non-invasive strategy to identify H. felis in dog and cats.

Biophysical Characteristics of Meridian System with Two Pain Diseases (통증에 따른 경락의 생체 물리적 정보 분석 연구)

  • Kaptchuk, Ted J.;Nam, Bong-Hyun
    • Korean Journal of Acupuncture
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    • v.22 no.4
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    • pp.29-41
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    • 2005
  • Objectives : Although previous anatomic, physiological and biophysics studies have examined the acupuncture meridian system, much remains unclear and controversial. This study was undertaken to examine electrical potential aspects of the meridian system. Electric potential was measured at the well and sea acupoints on the twelve acupuncture meridians (AM), on forty patients half with loin lesions, and pain of loin and lower extremities(LL) and half with shoulder lesions, and aching of shoulder and arm(SA). The object was to determine to what extent electric potential is an important risk factor between LL and SA. Methods : At the left and the right side with each of twenty LL and twenty SA patients, physiograph was used to measure electric potentials of AM ten sessions. T-test was used to compare the mean of electric potential between the two different pain groups and multiple logistic regression was used to analyze the risk of the 24 electric potentials measured. Results and Conclusions : In the LL, the only electric potential that was statistically significantly greater than SA was the bladder meridian on the left side. On the contrary, electric potentials in SA, which includes the large intestine, pericardium, triple burner, spleen, stomach, kidney and gallbladder meridians, were statistically larger than those of LL at the same side. On the right side, the five kinds of electric potentials(lung, large intestine, small intestine, pericardium and gallbladder meridian) of LL were statistically larger than those of SA. On the triple burner, stomach and kidney meridians electric potentials of SA were larger than those in LL. After adjusting for 24 electric potentials, pain risk factors, and different illness categories, multiple stepwise selection logistic regression modeling, resulted in the final selection of a total of 13 statistically significant electric potentials. These were 7 electric potentials at left side - small intestine, triple burner, spleen, stomach, bladder, liver and gallbladder meridian, and 6 at rght side - lung, large intestine, heart, pericardium, kidney and bladder meridian.

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The Effects of Sa-Am Acupuncture on Radial Pulse in Healthy Subjects: A Comparative Study of Large Intestine Tonifying and Sedating (대장정격 및 승격 자침이 정상 성인의 맥파에 미치는 영향 비교 연구)

  • Cho, Eun;Lee, Hyun
    • Journal of Acupuncture Research
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    • v.31 no.3
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    • pp.7-18
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    • 2014
  • Objectives : The purpose of this study is to find parameters to compare the effects of Sa-Am acupuncture with large intestine tonifying and sedating on radial pulse in healthy subjects. Methods : Sixty healthy subjects participated in this study, divided into large intestine tonifying acupuncture group, large intestine sedating acupuncture group and control group. Radial pulse was measured by 3 dimensional pulse imaging system(DMP-3000) before, right after, 30 minutes after and 60 minutes after acupuncture at Cun, Guan, and Chi in the acupuncture group and in the control group at the same time points. Results : 1. Angle of main peak and magnitude of fourier component significantly changed by integrated analysis of Cun, Guan and Chi. 2. Amplitude of H1, Pulse Power volume / min, elasticity, AIx / HR, Frequency and magnitude of fourier component significantly changed by analysis of Cun, Guan, and Chi. Conclusions : The effect of Sa-Am acupuncture with large intestine tonifying and sedating in healthy human may be observed on time, amplitude, pulse area, augmentation index and fourier components parameters. The parameters analysed in this study may be used to differentiate the effects between Sa-Am acupuncture with large intestine tonifying and sedating on radial pulse. Further studies on the effects of Sa-Am acupuncture using radial pulse are needed.

Preparation and Characterization of Sodium Alginate/PEO and Sodium Alginate/PVA Nanofiber (알긴산나트륨/PEO, 알긴산나트륨/PVA 나노섬유의 제조 및 특성분석)

  • Park, Ko-Eun;Park, Su-A;Kim, Geun-Hyung;Kim, Wan-Doo
    • Polymer(Korea)
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    • v.32 no.3
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    • pp.206-212
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    • 2008
  • Alginate obtained from marine brown algae, is a copolymer with repeating units of $\alpha$-($1{\rightarrow}4$)-L-guluronic acid(G) and $\beta$-($1{\rightarrow}4$)-D-mannuronic acid(M). It has good properties such as biocompatibility, non-toxicity. and hydrophilicity. However, alginate alone cannot be electrospun due to high viscosity and conductivity. To solve this problem. electro spinning of sodium alginate(SA) was performed by blending with poly(ethylene oxide)(PEO) and poly(vinyl alcohol)(PVA) in this study. Characteristics of SA/PEO nanofibers and SA/PVA nanofibers were estimated by SEM and XRD analyses. Optimal nanofiber webs are obtained from 2/2 wt% of SA/PEO and 2/7 wt% of SA/PVA. SA/PEO and SA/PVA nanofiber webs may have potentials for tissue engineering scaffold and wound dressing.

Influence of Plant Growth Regulator Application on Seed Germination of Dandelion (Taraxacum officinale) (식물생장조절물질 처리가 서양민들레 종자 발아에 미치는 영향)

  • Kim, Yoon Ha;Lee, In Jung
    • Weed & Turfgrass Science
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    • v.2 no.2
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    • pp.152-158
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    • 2013
  • Dandelion (Taraxacum officinale) is a member of family Asteraceae that grows all over the Korea. Recently, dandelion was cultivated for medicinal crops because of its positive medicinal effects. However, dandelion is considered as a troublesome weed in grass lawns of golf course and orchards. This study was conducted to investigate the effect of plant growth regulators [gibberellins ($GA_3$); kinetin; salicylic acid (SA); ethephon)] with different concentration on seed germination control of dandelion. Seed germination rates were increased in all concentration of $GA_3$ and kinetin treatment compared to control. In the 0.5 mM of ethephon application, seed germination rate was more increased than that of control while seed germination rate was reduced in 1.0 and 1.5 mM of ethephon treatments. Seed germination rate was significantly decreased with different SA dilutions compared to control. The germination rate was more reduced when SA was applied in combination with $GA_3$ than only SA treatments.

A Study on the Estimation of Watter Loss Rates in Irrigation Canals (灌漑用水路의 水路損失率 算定에 關한 硏究)

  • Koo, Ja-Woong;Lee, Ki-Chun;Kim, Jae-Young;Lee, Jae-Young
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.24 no.2
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    • pp.56-66
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    • 1982
  • This study was carried out in order to estimate water losses in irrigation canals, which may be used to evaluate the water requirement for irrigation projects. The conveyance losses were measured by the inflow-outflow method, the seepage losses were measured by the ponding method, and the operation losses in the course of irrigation were calculated by comparing the two kinds of losses. The results obtained in this experiment were as follows; 1. Conveyance losses per unit area of wetted perimeter by the main irrigation canal, the secondary irrigation canal and the tributary irrigation canal, were 1.399${\times}10^{-5}m^3/sec/m^2$, 5.154${\times}10^{-5}m^3/sec/m^2$, and 2.67${\times}10^{-5}m^3/sec/m^2$ respectively in the Goong-sa area. And they were 1.934${\times}10^{-5}m^3/sec/m^2$, 2.149${\times}10^{-5}m^3/sec/m^2$, and 4.558${\times}10^{-5}m^3/sec/m^2$ respectively in the Seong-dug area. 2. Seepage losses per unit area of wetted perimeter by the secondary irrigation canal and the tributary irrigation canal, were 2.180${\times}10^{-6}m^3/sec/m^2$ and 2.168${\times}10^{-6}m^3/sec/m^2$ in the Goong-sa area, 1.150${\times}10^{-6}m^3/sec/m^2$ and 1.084${\times}10^{-6}m^3/sec/m^2$ in the Seong-dug area respectively. 3. Operation losses per unit area of wetted perimeter by the secondary irrigation canal and the tributary irrigation canal, were 4.936${\times}10^{-5}m^3/sec/m^2$ and 2.453${\times}10^{-5}m^3/sec/m^2$ in the Goong-sa area, 2.034${\times}10^{-5}m^3/sec/m^2$ and 4.450${\times}10^{-5}m^3/sec/m^2$ in the Seong-dug area respectively. 4. Conveyance, seepage and operation losses in the Goong-sa area were 6.7%, 94.6%, and 14.0% more than those in the Seong-dug area. Operation losses amount to about 17 times as much as seepage losses in the Goong-sa area and about 29 times in the Seong-dug area. 5. The seepage losses depend much on the soil texture, ranging from 7.437${\times}10^{-7}m^3/sec/m^2$ to 2.430${\times}10^{-6}m^3/sec/m^2$. 6. Water loss rates in the main irrigatin canal, the secondary irrigation canal and the tributary irrigation canal, were estimated as 8.49%, 37.27% and 9.81% respectively in the Goong-sa area. And they were estimated as 15.10%, 32.67% and 13.78% respectively in the Seong-dug area.

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Quantitatation of Benzoic and Sorbic Acids in Some Processed Foods of Korea (일부 한국 가공식품의 벤조산과 소르빈산의 함량 조사)

  • Mitra, Sanika;Han, Jang-Il;Lee, Ki-Teak;Kim, Seong-Ai
    • Food Science and Preservation
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    • v.16 no.6
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    • pp.789-796
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    • 2009
  • Benzoic acid (BA) and sorbic acid (SA) are the preservatives most commonly used in food. Although BA and SA are generally safe, some previous studies have shown that consumption of excessive amounts of these food additives can be a health hazard. The aim of this study was to determine the amounts of BA and SA in processed foods in Korea. Different brands of fruit juice, yogurt, cheese, dried fruits, jam, and margarine were purchased at a local market in Daejeon, Korea. Samples were analyzed by high-performance liquid chromatography (HPLC) using a UV detector. Chromatographic separation was achieved with a C18 column. Methanol acetate buffer (pH 4.4) at a 35:65 v/v ratio was used as the initial mobile phase to elute BA and SA. The detector wavelength was set at 254 nm. The average test results observed for BA concentrations in fruit juice, yogurt, cheese, dried fruits, jam, and margarine were $40.26{\pm}0.02$, $2.07{\pm}0.06$, $0.02{\pm}0.09$, $0.36{\pm}08$, $265.30{\pm}0.02$, and $27.34{\pm}0.08\;mg/kg$, respectively. Average concentrations of SA in these samples were $0.92{\pm}0.06$, $1.06{\pm}0.07$, $7.30{\pm}0.01$, $14.14{\pm}0.08$, $25.65{\pm}0.06$, and $4.81{\pm}0.07\;mg/kg$, respectively. Thus, the average levels of BA and SA in the studied food items were lower than the KFDA-permitted limits. Moreover, the estimated daily intake of both BA and SA by a typical consumer were below the maximum recommended daily values.

Risk Factor Analysis for $SaO_2$ Instability after Systemic-pulmonary Shunt (전신-폐 단락술 후 산소포화도의 불안정성의 위험인자 분석)

  • Jung Sung-Ho;Yun Sok-Won;Park Jung-Jun;Seo Dong-Man;Kim Young-Hwue;Ko Jae-Kon;Park In-Sook;Yun Tae-Jin
    • Journal of Chest Surgery
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    • v.38 no.4 s.249
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    • pp.277-283
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    • 2005
  • Arterial oxygen saturation $(SaO_2)$ instability frequently takes place after systemic-pulmonary shunt without shunt occlusion. We analyzed actual incidence and risk factors for $SaO_2$ instability after shunt operations, and possible mechanisms were speculated on. Material and Method: Ninety three patients, who underwent modified Blalock-Taussig shunt from January 1996 to December 2000, were enrolled in this study. Adequacy of shunt was verified in all patients, either by ensuing one ventricle or biventricular repair later on or by appropriate pulmonary artery growth on postoperative angiogram. Age, body weight, hemoglobin level at operation were 3 day to 36 years (median: 1.8 months), 2.5kg to 51kg (median: 4.1kg) and $10.7\~24.3$ gm/dL (median: 15.2 gm/dL) respectively. Preoperative diagnoses were functional single ventricle with pulmonary stenosis or atresia in 39, tetralogy of Fallot in 38 and pulmonary atresia with intact ventricular septum in 16. Pulmonary blood flow (PBF) was maintained pre-operatively by patent ductus or previous shunt in 64 and by forward flow through stenotic right ventricular outflow tract (RVOT) in 29. $SaO_2$ instability was defined as $SaO_2$ less than $50\%$ for more than 1 hour with neither anatomic obstruction of shunt nor respiratory problem. Result: 10 patients $(10.7\%)$ showed $SaO_2$ instability after shunt operation. After shunt occlusion was ruled out by echocardiogram, they received measures to lower pulmonary vascular resistance (PVR), which worked within a few hours in all patients. Risk factors for $SaO_2$ instability included older age at operation (p=0.039), lower preoperative $SaO_2$ (p=0.0001) and emergency operation (p=0.001). PBF through stenotic RVOT showed marginal statistical significance (p=0.065). Conclusion: $SaO_2$ instability occurs frequently after shunt operation, especially in patients with severe hypoxia pre-operatively or unstable clinical condition necessitating emergency operation. Temporary elevation of pulmonary vascular resistance is a possible mechanism in this specific clinical setting.