• Title/Summary/Keyword: SA/CA

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Induction of HaCaT Cell Apoptosis by Sodium Nitroprusside (Sodium Nitroprusside로 유발한 HaCaT Cell의 Apoptosis)

  • Park, Yuri;Moon, Cheol;Kim, Sa-Hyun;Lee, Pyeongjae
    • Korean Journal of Clinical Laboratory Science
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    • v.47 no.3
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    • pp.112-116
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    • 2015
  • Nitric Oxide (NO) has been known to play important physiological and pathological roles. In this study, Sodium nitroprusside (SNP), NO donor, induced the apoptosis of HaCaT cell, human spontaneous immortal keratinocyte, which was investigated through DAPI staining and cleavage of PARP and caspase-3 protein. However, the expression level of Bip and CHOP, involved in ER stress, was not significantly changed as compared to the control cell group. Recent studies have showed that SIRT1, $NAD^+$-dependent deacetylase, is the key protein that controls cell survival and death. SNP treatment suppressed the SIRT1 gene expression, which indicated that apoptosis induced by SNP could be implicated in SIRT1 down-regulation.

Biosynthesis of polyhydroxyalkanoate by mixed microbial cultures from hydrolysate of waste activated sludge (혼합미생물배양체를 이용한 폐활성슬러지 가용화 산물로부터 polyhydroxyalkanoate 생합성)

  • Park, Taejun;Yoo, Young Jae;Jung, Dong Hoon;Lee, Sun Hee;Rhee, Young Ha
    • Korean Journal of Microbiology
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    • v.53 no.3
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    • pp.200-207
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    • 2017
  • A new approach to the solubilization of waste activated sludge (WAS) using alginate-quaternary ammonium complex beads was investigated under controlled mild alkaline conditions. The complex beads were prepared by the reaction of sodium alginate (SA) with 3-(trimethoxysilyl)propyl-octadecyldimethylammonium chloride (TSA) in acid solution, followed by crosslinking with $CaCl_2$. Treatment of WAS with SA-TSA complex beads was effective for enhancing the efficacy of WAS solubilization. The highest value of soluble chemical oxygen demand (SCOD) concentration (3,900 mg/L) was achieved after 10 days of treatment with 30% (v/v) SA-TSA complex beads. The WAS solubilization efficacy of the complex beads was also evaluated by estimating the concentrations of volatile fatty acids (VFAs). The maximum value of VFAs was 2,961 mg/L, and the overall proportions of VFAs were more than 75% of SCOD. The main components of VFAs were acetic, propionic, iso-butyric, and butyric acids. These results suggest that SA-TSA complex beads might be useful for enhancing the solubilization of WAS. The potential use of VFAs as the external carbon substrate for the production of polyhydroxyalkanoate (PHA) by a mixed microbial culture (MMC) was also examined. The enrichment of PHA-accumulating MMC could be achieved by periodic feeding of VFAs generated from WAS in a sequencing batch reactor. The composition of PHA synthesized from VFAs mainly consisted of 3-hydroxybutyrate. The maximum PHA content accounted for 25.9% of dry cell weight. PHA production by this process is considered to be promising since it has a doubly beneficial effect on the environment by reducing the amount of WAS and concomitantly producing an eco-friendly biopolymer.

Effect of Na, K, Ca and Mg ions on the Action Potential of the Sinoatrial Node in the Rabbit (토끼 동방결절 활동전압에 대한 Na, K, Ca 및 Mg 이온의 영향)

  • Lee, Jeong-Ryeol;Eom, Yung-Ui
    • Journal of Chest Surgery
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    • v.18 no.1
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    • pp.1-6
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    • 1985
  • Isolated sinus node cells of the rabbit were used to assess the effects of extracellular Na, K, Ca and Mg concentrations on cardiac pacemaker activity. With intracellular glass micro-electrodes spontaneous action potentials of SA node were recorded and the effects of various ions and their blockers were analyzed in terms of the cycle length, the amplitude and the duration of action potentials, the results obtained were as follows. 1. Sodium reduction [up to 30%] decreased the amplitude of action potential and lengthened the cycle length. TTX, specific blocker of Na channel slightly lengthened the cycle length. 2. Increasing potassium ion concentration, the duration of action potential decreased and the frequency increased in 6mM, however, spontaneous action potential was stopped in 24 mM. Barium ion known to be decreasing K conductance increased the duration of action potential but no significant change in the cycle length was noticed. 3. Calcium ion has shortening effect on the duration and the cycle length of action potential but not with dose-dependent manner. Cadmium ion .[0.02mM] lengthened cycle length and the duration of action potential. 4. Increasing the concentration of magnesium ion the cycle length was lengthened, significantly.

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An Experimental Study on the Development of Sewage Sludge Artificial Light-weight Aggregate Using Pottery Stone (도석을 점결제로 상용한 하수슬러지 인공경량골재의 개발에 관한 실험적 연구)

  • Jeong, Ui-Seung;Sa, Soon-Heon;Ji, Suk-Won;Seo, Chee-Ho
    • Proceedings of the Korean Institute of Building Construction Conference
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    • 2010.05a
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    • pp.91-94
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    • 2010
  • The purpose of this study is to produce artificial lightweight aggregate. The properties of aggregate are deducted by analysing the plasticity of aggregate according to the addictive contents of $CaCO_3$ and $Al_2O_3$ on constant plastic temperature($1150^{\circ}C{\sim}1160^{\circ}C$) and the specific gravity, the percentage of water absorbtion. The density on the temperature of $1150^{\circ}C{\sim}1160^{\circ}C$ which results from that the plastic temperature of pottery stone is decreased by increasing the addictive contents of $CaCO_3$ and $Al_2O_3$ manufacturing artificial light weight aggregate using pottery stone is included in the arrange of light weight aggregate density. And the percentage of water absorbtion is 4.2~14% which is similar to or lower than existing artificial light weight aggregate. The unit volume weight is in inverse proportion to density and to increase addictive contents of flux.

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Properties with Annealing Temperature of $(Sr_{0.9}Ca_{0.1})TiO_{3}$ Ceramic Thin Film ($(Sr_{0.9}Ca_{0.1})TiO_{3}$ 세라믹 박막의 열처리온도에 따른 특성)

  • So, Byung-Moon;Cho, Choon-Nam;Shin, Cheol-Gi;Kim, Jin-Sa;Kim, Chung-Hyeok
    • The Transactions of the Korean Institute of Electrical Engineers C
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    • v.51 no.11
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    • pp.526-530
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    • 2002
  • The $(Sr_{0.9}Ca_{0.1})TiO_3(SCT)$ thin films were deposited on Pt-coated electrode (Pt/TiN/$SiO_2$/Si) using RF sputtering method. The composition of SCT thin film deposited on Si substrate at room temperature is close to stoichiometry(1.081 in A/B ratio). The maximum dielectric constant of SCT thin film was obtained by ammealing at $600^{\cric}C$. The temperature dependence of dielectric loss showed a value within 0.02 in temperature ranges of -80 ${\sim}$ +90$^{\circ}C$. The capacitance characteristics showed a stable value within ${\pm}$4%. The drastic decrease of dielectric constant and increase of dielectric loss in SCT thin films was observed at the frequency above 200kHz.

Capacitive-Voltage properties of$(Sr{\cdot}Ca)TiO_{3}$ Ceramics ($(Sr{\cdot}Ca)TiO_{3}$ 세라믹스의 용량-전압 특성)

  • Kang, Jae-Hun;Choi, Woon-Shik;Kim, Chung-Hyeok;Kim, Jin-Sa;Park, Yong-Pill;Song, Min-Jong
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2001.11b
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    • pp.34-37
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    • 2001
  • In this study, the capacitance-voltage properties of $(Sr_{1-x}\cdot Ca_x)TiO_3(0.05{\leq}x{\leq}0.20)$-based grain boundary layer ceramics were investigated. The ceramics were fabricated by the conventional mixed oxide method. The sintering temperature and time were $1480\sim1500^{\circ}C$ and 4 hours. respectively. The 2nd phase formed by the thermal diffusion of CuO from the surface leads to very excellent dielectric properties, that is, ${\varepsilon}_r$ >50000, tan$\delta$ <0.05, ${\Delta}C$ < ${\pm}10%.$ The capacitance is almost unchanged below about 20[V] but it decreases slowly about 20[V]. The results of the capacitance-voltage properties indicated that the grain boundary was composed of the continuous insulating layers.

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Deposition and Properties of Pt/ST/Pt Thin Film Structure (Pt/ST/Pt 소자 구조의 박막증착 및 특성)

  • Kim, Jin-Sa;Cho, Choon-Nam;Oh, Yong-Cheul;Shin, Cheol-Gi;Song, Min-Jong;So, Byeong-Mun;Choi, Woon-Shick;Kim, Chung-Hyeok
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2007.06a
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    • pp.472-473
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    • 2007
  • The $(Sr_{1-x}Ca_x)TiO_3$(ST) thin films are deposited on Pt-coated electrode(Pt/TiN/$SiO_2$/Si) using RF sputtering method with substitutional contents of Ca. The maximum grain of thin films is obtained by substitution of Ca at 15[mol%]. Also, the composition of ST thin films were closed to stoichiometry(1.081~1.117 in A/B ratio). The dielectric constant changes almost linearly in temperature ranges of -80~+90[$^{\circ}C$]. The current-voltage characteristics of ST15 thin films showed the increasing leakage current as the measuring temperature increases.

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Colonial Morphology of Yeasts Grown on the Medium Containing Pigments Extracted from the Fruits of Gardenia jasminoides (수용성 치자(水溶性 梔子)(Gardenia jasminoides열매)색소(色素)를 첨가(添加)한 배지(培地)에 배양(培養)한 각종(各種) 효모균류(酵母菌類) 균집락(菌集落)의 형태(形態))

  • Kim, Sang-Jae;Kim, Sin-Ok;Jeong, Suk
    • The Korean Journal of Mycology
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    • v.19 no.2
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    • pp.136-142
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    • 1991
  • Colonial morphology of the various yeasts grown on the yeast morphology agar me­dium containing orange-yellow pigments extracted from the fruits of Gardenia jasminoides (GJPM) was investigated in hopes of the differential identification of yeasts on primary cultures. Colonies of Candida lusitaniae and Ca. guilliermondii on GJPM turned to prussian blue within three days of incubation and Ca. tropicalis and Ca. viswanathii turned to bluish gray but the latter species turned to deep blue after 7 days. Ca. krusei, Saccharomyces cerevisiae, and Torulopsis glabrata showed neutral gray, grayish green, and baby blue respectively after one or two weeks. However, the colonies of Ca. albicans and parapsilosis remained unchanged even after 20 days. Colonial color of Cryptococ­cus neoformans showing brown to purple brown was distinguishable not only from buff color of Cr. laurentii after one or two weeks incubation but also from those of Candida spp. Growth of certain species was promoted on GJPM. The findings clearly showed that Ga. jasminoides pigments medium was useful to the morphological differentiation of medically important yeasts which were often encountered in sputum or other clinical specimens.

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Analysis of Chemical and Meteorological Effects on the Concentration Difference of Photochemical Air Pollutants between Coastal and Inland Regions in Busan (부산시 해안 및 내륙지역에서 광화학 오염물질의 농도 차이에 영향을 주는 화학 및 기상조건 분석)

  • Sang, Sang-Keun;Shon, Zang-Ho
    • Journal of Environmental Science International
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    • v.17 no.10
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    • pp.1169-1182
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    • 2008
  • The chemical and meteorological effects on the concentration variations of air pollutants ($O_3$ and its precursors) were evaluated based on ground observation data in coastal and inland regions, Busan during springs and summers of 2005-2006. For the purpose of this study, study areas were classified into 5 categories: coastal area (CA), industrial area (IA), downtown area (DA), residential area (RA), and suburban area (SA). Two sites of Dongsam (DS) and Yeonsan (YS) were selected for the comparison purpose between the coastal and inland regions. $O_3$ concentrations in CA and SA were observed to be highest during spring (e.g., 40 ppb), whereas those in DA and RA were relatively low during summer (e.g., $22\sim24$ ppb). It was found that $O_3$ concentrations in IA were not significantly high although high VOCs (especially toluene of about 40 ppb) and $NO_x$ ($\geq$ 35 ppb) were observed. On the other hand, the concentration levels of $O_3$ and $PM_{10}$ at the DS site were significantly higher than those at the YS site, but $NO_x$ was slightly lower than that at the YS site. This might be caused by the photochemical activity and meteorological conditions (e.g., sea-land breeze and atmospheric stagnance). When maximum $O_3$ (an index of photochemical activity) exceeds 100 ppb, the contribution of secondary $PM_{10}\;((PM_{10})_{SEC})$ to total observed $PM_{10}$ concentrations was estimated up to 32% and 17% at the DS and YS sites, respectively. In addition, the diurnal variations of $(PM_{10})_{SEC}$ at the DS site were similar to those of $O_3$ regardless of season, which suggests that they are mostly secondary $PM_{10}$ produced from photochemical reactions.