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Effect of Continuous Feeding of Probiotics on Growth Performance, Nutrient Digestibility, Blood Urea Nitrogen and Immune Responses in Pigs (지속적인 생균제의 첨가가 돼지의 성장, 영양소 이용율, 혈중 요소태 질소 및 면역능력에 미치는 영향)

  • Kil, D.Y.;Lim, J.S.;Tian, J.Z.;Kim, B.G.;Kim, K.S.;Kim, Y.Y.
    • Journal of Animal Science and Technology
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    • v.46 no.1
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    • pp.39-48
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    • 2004
  • This experiment was conducted to investigate the effect of continuous feeding of probiotics on growth performance, nutrient digestibility, blood urea nitrogen(BUN) and immune responses in pigs. Treatments were 1) Control(basal diet), 2) P-O.l(basal diet + 0.1% probiotics) and 3) P-0.2(basal diet + 0.2% probiotics). In growth trial, a total of sixty pigs(6.17 $\pm$ 0.45 kg average body weight) weaned at 21 days of age were used. All pigs were assigned according to sex and body weight, and each treatment had 5 replicates of 4 pigs per pen in a randomized complete block(RCB) design. During 0${\sim}$8 weeks, there was no significant difference in average daily gain(ADG), average daily feed intake(ADFI) and gain:feed ratio(GfF) among treatments. During 9 - 20 weeks, ADG was improved significantly in pigs fed P-O.I or P-0.2 diets when compared to the pig fed control diet(P <0.05), but there was no significant difference in ADFI and GfF ratio. During overall period, ADG, ADFI and GfF ratio were not significantly different among treatments. In the first metabolic trial(17.93 $\pm$1.45kg average body weight), apparent digestibility of OM, protein, fat in pigs fed P-O.l and P-0.2 diets were greater than in pigs fed control diet(P <0.05) and ash digestibility in pigs fed P-0.2 diet was significantly higher than in pigs fed control diet(P <0.05). Calcium digestibility in pigs fed P-0.2 diet was significantly higher than in pigs fed control and P-O.I diets(P <0.05). Fecal-N excretion was lower in pigs fed P-O.! and P-0.2 diets than in pigs fed control(P <0.05). In the second metabolic trial(41.80 $\pm$ 2.68kg average body weight), there was no significant difference among treatments in apparent digestibility of nutrients and N-retention. In blood assay for the BUN and immune responses investigations, there was no significant difference among treatments during overall period of experiment. Therefore, this experiment suggested that probiotics supplementation could improve growth performance and nutrient digestibility of pigs.

Evaluation of Maternal Behavior between Normal Parturition and Expected Cesarean Section in Rats (자연 분만 및 예정된 제왕절개 수술 랫드에 있어서 모성 행동의 차이에 대한 검토)

  • Lee, S.K.;Kang, H.G.;Kim, I.W.;Jeong, J.M.;Hwang, D.Y.;Kim, C.K.;Chae, K.R.;Cho, J.S.
    • Journal of Embryo Transfer
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    • v.22 no.3
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    • pp.161-165
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    • 2007
  • Oxytocin is a neurohypophyseal hormone which has multiple functions in mammals. Mainly, oxytocin regulates milk ejection and has an effect on uterine contraction and is related to maternal behavior. Maternal behavior is believed to be suppressed by stress and facilitated by oxytocin. In the cesarean section, oxytocin may be administrated into uterus to promote uterine involution. The present study aimed to test the effect of oxytocin into uterus on maternal behavior of rats with cesarean section. It was measured the effects on maternal behavior of oxytocin infused into uterus in rats with cesarean section as a stressor. In the first experiment, pup survival rate of between a control group and a group with laparotomy as a stress in natural parturition rats was compared. In the second experiment, survival rate for 2 weeks and maternal pup searching behavior (MPSV) were observed in one cesarean sectioned group without oxytocin and the other cesarean sectioned group with oxytocin. Infanticide was observed in stressed group in the first experiment while a normal maternal behavior was observed in a control one. In the second experiment, MPSV was only observed in a cesarean sectioned group with oxytocin and infanticide was observed in two groups except one rat which is thought to be affected by oxytocin as operated relatively late. This is the first study to show that the administration of oxytocin into uterus in the cesarean section is not involved in the regulation of maternal behavior in rats. In conclusion, this study proves the needs of oxytocin into brain in cesarean section related rats model and further study of maternal behavior list, like MPSV.

Evaluation of Image Quality Change by Truncated Region in Brain PET/CT (Brain PET에서 Truncated Region에 의한 영상의 질 평가)

  • Lee, Hong-Jae;Do, Yong-Ho;Kim, Jin-Eui
    • The Korean Journal of Nuclear Medicine Technology
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    • v.19 no.2
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    • pp.68-73
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    • 2015
  • Purpose The purpose of this study was to evaluate image quality change by truncated region in field of view (FOV) of attenuation correction computed tomography (AC-CT) in brain PET/CT. Materials and Methods Biograph Truepoint 40 with TrueV (Siemens) was used as a scanner. $^{68}Ge$ phantom scan was performed with and without applying brain holder using brain PET/CT protocol. PET attenuation correction factor (ACF) was evaluated according to existence of pallet in FOV of AC-CT. FBP, OSEM-3D and PSF methods were applied for PET reconstruction. Parameters of iteration 4, subsets 21 and gaussian 2 mm filter were applied for iterative reconstruction methods. Window level 2900, width 6000 and level 4, 200, width 1000 were set for visual evaluation of PET AC images. Vertical profiles of 5 slices and 20 slices summation images applied gaussian 5 mm filter were produced for evaluating integral uniformity. Results Patient pallet was not covered in FOV of AC-CT when without applying brain holder because of small size of FOV. It resulted in defect of ACF sinogram by truncated region in ACF evaluation. When without applying brain holder, defect was appeared in lower part of transverse image on condition of window level 4200, width 1000 in PET AC image evaluation. With and without applying brain holder, integral uniformities of 5 slices and 20 slices summation images were 7.2%, 6.7% and 11.7%, 6.7%. Conclusion Truncated region by small FOV results in count defect in occipital lobe of brain in clinical or research studies. It is necessary to understand effect of truncated region and apply appropriate accessory for brain PET/CT.

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Competition between Transplanted Lowland Rice and Weeds as Affected by Plant Spacing and Rice Cultivar Having Different Maturity (수도품종(水稻品種)의 숙기(熟期) 차이(差異)가 잡초(雜草)와의 경쟁력(競爭力)에 미치는 영향(影響))

  • Kim, S.C.;Kim, J.K.;Kim, D.S.
    • Korean Journal of Weed Science
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    • v.2 no.1
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    • pp.7-12
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    • 1982
  • The experiment was studied to know the competitive ability of rice against weeds as affected by rice maturity and plant spacing. For both cultivars, Tongil (12 days later) and Yeongnamjosaeng, the least amount of weed was obtained from the 10 ${\times}$ 10cm plant spacing while no difference between 40 ${\times}$ (10 ${\times}$ 10cm) and 30 ${\times}$ 15cm plant spacings was observed. When weed was nor pulled out by hand, significant yield loss was resulted in 30 ${\times}$ 15cm and 40 ${\times}$ (10 ${\times}$ 10cm) plant spacings while no difference between weeding regime was observed at the 10 ${\times}$ 10cm plant spacing in both cultivars. Rice grain yield was unaffected by plant spacing for hand weeded plot in both rice cultivars. For noweeding plot, however, the highest grain yield was obtained from 10 ${\times}$10cm plant spacing and also this yield was not significantly different from the yield of hand weeded plot while significant yield loss was recognized at the 30 ${\times}$ 15cm and 40 ${\times}$ (10 ${\times}$ 10cm) plant spacings in noweeding plot. There was also a good relationship between weed weight and grain yield for both cultivars (Tongil; Y = 5.68 - 0.0127 X, r = -0.814$^*$; Yeongnamjosaengv Y = 5.04 - 0.0314 X, r =-0.9704$^{**}$). Based on these relationship, weed weight of 188.2g/sq.m for Yeongnamjosaeng and 223.6g/sq.m for Tongil was needed to reduce rice yield by 50%, respectively. This result implied that Tongil compete more efficiently against weed than Yeongnamjosaeng and hence, could be concluded that late maturing cultivar was more competitive against weed compared to early maturing cultivar.

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Effects of CLA-vegetable Oils and CLA-lard Additives on Quality Characteristics of Emulsion-type Sausage (유화형 Sausage의 품질특성에 식물성유와 동물성유 CLA 첨가가 미치는 영향)

  • Lee, J. I.;Lee, J. H.;Kwack, S. C.;Ha, Y. J.;Jung, J. D.;Lee, J. W.;Lee, J. R.;Joo, S. T.;Park, G. B.
    • Journal of Animal Science and Technology
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    • v.45 no.2
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    • pp.283-296
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    • 2003
  • Emulsion-type sausages were manufactured to investigate the effects of CLA-vegetable oils and CLA-lard on quality characteristics of emulsion-type sausage. Each treatments replaced pork back fat with CLA-corn oil (CLA-CO), CLA-safflower seed oil (CLA-SSO) and CLA-lard (CLA-LD) were stored during 1, 7, 14, 21 and 28 days at 4$^{\circ}C$. The changes in physico-chemical properties, texture, thiobarbituric acid reactive substances (TBARS), fatty acid composition and CLA content of each treatments were measured during 1, 7, 14, 21 and 28 days at 4$^{\circ}C$. During storage, pH of control showed higher pH value than that of CLA-vegetable and CLA-lard treatments. The pH values of all treatments was significantly low at storage 14 days (p<0.05). The color L* and a*-value of all the treatments was not significantly changed with the storage periods. Color b*-value of CLA-treatments was higher than that of control during the storage period. Fatty acids composition was changed by addition of CLA-vegetable oils and CLA-lard. All kinds of fatty acids composition were decreased with CLA replacements than that of the control. Whereas linolenic acid content extremely increased by replacement of CLA-vegetable. CLA content of CLA-treatments was extremely increased than that of the control. The cohesiveness, springness, gumminess and brittleness of CLA-LD treatment was significantly higher (P<0.05) than that of the other treatments. The TBARS value of all treatments were significantly increased (P<0.05) as the storage period was passed. TBARS of sausage products containing CLA-CO was the lowest. Summing up the a forementioned results, emulsion-type sausage manufacturing with CLA-vegetable oil and CLA-lard was not affected in physico-chemical properties and texture characteristics. Also, it may be assumed that the high quality sausage can be manufactured with the extent of storage period and CLA accumulation.

A Destructive Method in the Connection of the Algorithm and Design in the Digital media - Centered on the Rapid Prototyping Systems of Product Design - (디지털미디어 환경(環境)에서 디자인 특성(特性)에 관한 연구(硏究) - 실내제품(室內製品) 디자인을 중심으로 -)

  • Kim Seok-Hwa
    • Journal of Science of Art and Design
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    • v.5
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    • pp.87-129
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    • 2003
  • The purpose of this thesis is to propose a new concept of design of the 21st century, on the basis of the study on the general signification of the structures and the signs of industrial product design, by examining the difference between modern and post-modern design, which is expected to lead the users to different design practice and interpretation of it. The starting point of this study is the different styles and patterns of 'Gestalt' in the post-modern design of the late 20th century from modern design - the factor of determination in industrial product design. That is to say, unlike functional and rational styles of modern product design, the late 20th century is based upon the pluralism characterized by complexity, synthetic and decorativeness. So far, most of the previous studies on design seem to have excluded visual aspects and usability, focused only on effective communication of design phenomena. These partial studies on design, blinded by phenomenal aspects, have resulted in failure to discover a principle of fundamental system. However, design varies according to the times; and the transformation of design is reflected in Design Pragnanz to constitute a new text of design. Therefore, it can be argued that Design Pragnanz serves as an essential factor under influence of the significance of text. In this thesis, therefore, I delve into analysis of the 20th century product design, in the light of Gestalt theory and Design Pragnanz, which have been functioning as the principle of the past design. For this study, I attempted to discover the fundamental elements in modern and post-modern designs, and to examine the formal structure of product design, the users' aesthetic preference and its semantics, from the integrative viewpoint. Also, with reference to history and theory of design my emphasis is more on fundamental visual phenomena than on structural analysis or process of visualization in product design, in order to examine the formal properties of modern and post-modern designs. Firstly, In Chapter 1, 'Issues and Background of the Study', I investigated the Gestalt theory and Design Pragnanz, on the premise of formal distinction between modern and post-modern designs. These theories are founded upon the discussion on visual perception of Gestalt in Germany in 1910's, in pursuit of the principle of perception centered around visual perception of human beings. In Chapter 2, I dealt with functionalism of modern design, as an advance preparation for the further study on the product design of the late 20th century. First of all, in Chapter 2-1, I examined the tendency of modern design focused on functionalism, which can be exemplified by the famous statement 'Form follows function'. Excluding all unessential elements in design - for example, decoration, this tendency has attained the position of the international style based on the spirit of Bauhause - universality and regularity - in search of geometric order, standardization and rationalization. In Chapter 2-2, I investigated the anthropological viewpoint that modern design started representing culture in a symbolic way including overall aspects of the society - politics, economics and ethics, and its criticism on functionalist design that aesthetic value is missing in exchange of excessive simplicity in style. Moreover, I examined the pluralist phenomena in post-modern design such as kitsch, eclecticism, reactionism, hi-tech and digital design, breaking away from functionalist purism of modern design. In Chapter 3, I analyzed Gestalt Pragnanz in design in a practical way, against the background of design trends. To begin with, I selected mass product design among those for the 20th century products as a target of analysis, highlighting representative styles in each category of the products. For this analysis, I adopted the theory of J. M Lehnhardt, who gradated in percentage the aesthetic and semantic levels of Pragnantz in design expression, and that of J. K. Grutter, who expressed it in a formula of M = O : C. I also employed eight units of dichotomies, according to the G. D. Birkhoff's aesthetic criteria, for the purpose of scientific classification of the degree of order and complexity in design; and I analyzed phenomenal aspects of design form represented in each unit. For Chapter 4, I executed a questionnaire about semiological phenomena of Design Pragnanz with 28 units of antonymous adjectives, based upon the research in the previous chapter. Then, I analyzed the process of signification of Design Pragnanz, founded on this research. Furthermore, the interpretation of the analysis served as an explanation to preference, through systematic analysis of Gestalt and Design Pragnanz in product design of the late 20th century. In Chapter 5, I determined the position of Design Pragnanz by integrating the analyses of Gestalt and Pragnanz in modern and post-modern designs In this process, 1 revealed the difference of each Design Pragnanz in formal respect, in order to suggest a vision of the future as a result, which will provide systemic and structural stimulation to current design.

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Within Field Distribution Pattern and Design of a Sampling Plan for Damaged Onions by the Onion maggot, Hylemya antiqua Meigen(Diptera: Anthomyiidae) (고자리파리에 의한 양파피해(被害)의 포장내(圃場內) 분포양식(分布樣式)과 피해량(被害量) 추정(推定)을 위한 표본추출(標本抽出) 계획(計劃))

  • Park, C.G.;Hyun, J.S.;Cho, D.J.;Lee, K.S.;Hah, J.K.
    • Korean journal of applied entomology
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    • v.24 no.1 s.62
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    • pp.29-33
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    • 1985
  • Every plant in $990m^2$ onion field was inspected for damages by the onion maggot. Maps were constructed every ten days to show which plants were infested and which were not from April 11 to May 21, 1984. The maps were sectioned into squares one of which contains 80 onion plants and the counts of damaged onions in each square were fitted to poisson and negative binomial distribution and tested by chi-square. We argue that the satisfactory fitness of the expected negative binomial $[P(x^2)>0.05]$ provided a useful description of the spatial distribution patterns of the damaged onions. Edge effect was tested by the differences of damage ratio and variance/mean ratio (${\sigma}^2/m$) between edge and center part. The result showed that the damage ratioes and variances of all the periods, ${\sigma}^2/m$ values after May 1 were greater in edge part than in center part. Again, the maps were sectioned into four blocks and the squares (sample units) were sectioned into quadrants. By application of the variance component technique, it was suggested that $2{\sim}8$ sample units for 5% sampling error and $1{\sim}2$ sample units for 10% error should be sampled randomly to estimate the damage ratio when $2{\sim}3$ quadrants were inspected.

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Expression Pattern of Skeletal-Muscle Protein Genes and Cloning of Parvalbumin mRNA in Dark-banded Rockfish (Sebastes inermis) (볼락(Sebastes inermis) 근육단백질 유전자의 성장단계별 발현 양상과 parvalbumin 유전자 클로닝)

  • Jang, Yo-Soon
    • Korean Journal of Ichthyology
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    • v.23 no.1
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    • pp.1-9
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    • 2011
  • Differentially Expressed Gene (DEG) was obtained from Differential Display Reverse Transcription (DDRT)-PCR using Annealing Control Primer (ACP) to search and clone genes related to developmental stages of Sebastes inermis. By using 120 ACPs, the nucleotide sequences obtained from 16 DEGs showing higher expression in 6-month-old skeletal muscle than 18-month-old ones and from 22 DEGs displaying stronger expression in 18-month-old than 6-month-old were analyzed and BLAST was conducted. The results identified that DEGs shared 69~95% homology with genes of parvalbumin (PVALB), nucleoside diphosphate kinase (NDK) B, tropomyosin (TPM), troponin I (TnI), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), muscle-type creatine kinase (CKM2), small EDRK-rich factor 2 (SERF2), adenosine monophosphate deaminase (AMPD), Trimeric intracellular cation channel type A (TRICA), Rho GTPase-activating protein 15 (ARHGAP15), S-formylglutathione hydrolase (Esterase D; ESD), heat shock protein 70 (hsp70), type 1 collagen alpha 2 (COL1A2), glutathione S-transferase, Mid1-interacting protein 1 (Mid1lip1), myosin light chain 1 (MYL1), sarcoplasmic/endoplasmic reticulum calcium ATPase 1B (SERCA1B), and ferritin heavy subunit (FTH1). Expression pattern by developmental stage of DEG14 and PVALB exhibiting strong expression in 6-month-old skeletal muscle was investigated using real time PCR. Expression was reduced as Sebastes inermis grew. Expression of PVALB gene was extremely low after 6 months of age. Expression of CKM2 showed higher expression in 18-month-old skeletal muscle than in 6-month-old muscles, and increased continuously until 4 years old, after which CKM2 expression became gradually reduced. By analysis of tissue-specific expression patterns of DEG, DEG14 was expressed mainly in skeletal muscle, liver, kidney and spleen tissues, whereas PVALB expression was expressed in skeletal muscle and kidney, but not in liver and spleen tissues. CKM2 was expressed in skeletal muscle, kidney, and spleen tissues, but not in liver tissues. PVALB gene was composed of 110 amino acids, which constituted 659 bp nucleotides. The results reported here demonstrate that the expression patterns of parvalbumin and CKM2 could be used as molecular markers for selecting fishes exhibiting fast growth.

Seismic Data Processing and Inversion for Characterization of CO2 Storage Prospect in Ulleung Basin, East Sea (동해 울릉분지 CO2 저장소 특성 분석을 위한 탄성파 자료처리 및 역산)

  • Lee, Ho Yong;Kim, Min Jun;Park, Myong-Ho
    • Economic and Environmental Geology
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    • v.48 no.1
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    • pp.25-39
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    • 2015
  • $CO_2$ geological storage plays an important role in reduction of greenhouse gas emissions, but there is a lack of research for CCS demonstration. To achieve the goal of CCS, storing $CO_2$ safely and permanently in underground geological formations, it is essential to understand the characteristics of them, such as total storage capacity, stability, etc. and establish an injection strategy. We perform the impedance inversion for the seismic data acquired from the Ulleung Basin in 2012. To review the possibility of $CO_2$ storage, we also construct porosity models and extract attributes of the prospects from the seismic data. To improve the quality of seismic data, amplitude preserved processing methods, SWD(Shallow Water Demultiple), SRME(Surface Related Multiple Elimination) and Radon Demultiple, are applied. Three well log data are also analysed, and the log correlations of each well are 0.648, 0.574 and 0.342, respectively. All wells are used in building the low-frequency model to generate more robust initial model. Simultaneous pre-stack inversion is performed on all of the 2D profiles and inverted P-impedance, S-impedance and Vp/Vs ratio are generated from the inversion process. With the porosity profiles generated from the seismic inversion process, the porous and non-porous zones can be identified for the purpose of the $CO_2$ sequestration initiative. More detailed characterization of the geological storage and the simulation of $CO_2$ migration might be an essential for the CCS demonstration.

An International Collaborative Program To Discover New Drugs from Tropical Biodiversity of Vietnam and Laos

  • Soejarto, Djaja D.;Pezzuto, John M.;Fong, Harry H.S.;Tan, Ghee Teng;Zhang, Hong Jie;Tamez, Pamela;Aydogmus, Zeynep;Chien, Nguyen Quyet;Franzblau, Scott G.;Gyllenhaal, Charlotte;Regalado, Jacinto C.;Hung, Nguyen Van;Hoang, Vu Dinh;Hiep, Nguyen Tien;Xuan, Le Thi;Hai, Nong Van;Cuong, Nguyen Manh;Bich, Truong Quang;Loc, Phan Ke;Vu, Bui Minh;Southavong, Boun Hoong
    • Natural Product Sciences
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    • v.8 no.1
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    • pp.1-15
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    • 2002
  • An International Cooperative Biodiversity Group (ICBG) program based at the University of Illinois at Chicago initiated its activities in 1998, with the following specific objectives: (a) inventory and conservation of of plants of Cuc Phuong National Park in Vietnam and of medicinal plants of Laos; (b) drug discovery (and development) based on plants of Vietnam and Laos; and (c) economic development of communities participating in the ICBG project both in Vietnam and Laos. Member-institutions and an industrial partner of this ICBG are bound by a Memorandum of Agreement that recognizes property and intellectual property rights, prior informed consent for access to genetic resources and to indigenous knowledge, the sharing of benefits that may arise from the drug discovery effort, and the provision of short-term and long-term benefits to host country institutions and communities. The drug discovery effort is targeted to the search for agents for therapies against malaria (antimalarial assay of plant extracts, using Plasmodium falciparum clones), AIDS (anti-HIV-l activity using HOG.R5 reporter cell line (through transactivation of the green fluorescent protein/GFP gene), cancer (screening of plant extracts in 6 human tumor cell lines - KB, Col-2, LU-l, LNCaP, HUVEC, hTert-RPEl), tuberculosis (screening of extracts in the microplate Alamar Blue assay against Mycobacterium tuberculosis $H_{37}Ra\;and\;H_{37}Rv),$ all performed at UIC, and CNS-related diseases (with special focus on Alzheimer's disease, pain and rheumatoid arthritis, and asthma), peformed at Glaxo Smith Kline (UK). Source plants were selected based on two approaches: biodiversity-based (plants of Cuc Phuong National Park) and ethnobotany-based (medicinal plants of Cuc Phuong National Park in Vietnam and medicinal plants of Laos). At mc, as of July, 2001, active leads had been identified in the anti-HIV, anticancer, antimalarial, and anti- TB assay, after the screening of more than 800 extracts. At least 25 biologically active compounds have been isolated, 13 of which are new with anti-HIV activity, and 3 also new with antimalarial activity. At GSK of 21 plant samples with a history of use to treat CNS-related diseases tested to date, a number showed activity against one or more of the CNS assay targets used, but no new compounds have been isolated. The results of the drug discovery effort to date indicate that tropical plant diversity of Vietnam and Laos unquestionably harbors biologically active chemical entities, which, through further research, may eventually yield candidates for drug development. Although the substantial monetary benefit of the drug discovery process (royalties) is a long way off, the UIC ICBG program provides direct and real-term benefits to host country institutions and communities.