• Archives of Pharmacal Research
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• v.15 no.1
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• pp.30-34
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• 1992

In order to obtain acetaminophen, a popular analgesic-antipyretic, through microbial p-hydroxylation and N-acetylation of aniline, various fungi and bacteria were secreened. Among them, Streptomyces species were chosen for strain improvement by the use of interspecific protoplast fusion technique. Two interspecific fused strains were developed between S. rimosus (N-cetylation function) and S. aureofaciens (p-hydroxylation function) and also between S. lividans and S. globisporus. For efficient protoplast fusion and cell wall regeneration, various conditions were examined. In a typical experiment of mixed S rimosus ($pro^- \;his^-$) and S. aureofaciens ($ilv^-$) protoplasts with 40% (w/v) polythylene glycol 3350 (PEG) for 3 min gave $8.3\times10^{-7}$ of fusion frequency. Treatment of mixed S. lividans (pant-) and S. globisporus (leu-) protoplasts with 50% (w/v) PEG for 3 min at $30^\circ{C}$ gave $1.2\times10^{-6}$ of frequency. Among the fused strains, up to 40-50% increase in p-hydroxylation power was observed. To investigate the possibility of plasmid involvement in p-hydroxylation power was observed. To investigate the possibility of plasmid involvement in p-hydroxylation of acetanilide, plasmid curing was attempted. We found that cells treated with acriflavine (at the frequency of 100%) and cells regenerated from protoplsts of S. auroefaciens (2% frequency) lost their p-hydroxylation function.

• Journal of Environmental Health Sciences
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• v.22 no.3
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• pp.1-7
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• 1996

Polychlorinated Biphenyls(PCBs)의 생물학적 처리가 시도되고 있으며, PCBs를 분해할 수 있는 미생물을 이용할 수 있다. 따라서 본 연구에서는 폐기된 절연유의 생물학적 처리를 위하여 PCBs를 분해하는 균을 분해하였으며, 분해된 균을 이용하여 절연유 내의 Polychlorinated Biphenyls(PCBs) 분해를 회분식 실험에서 연구하였다. 대구의 신천으로부터 유일한 탄소원으로 Biphenyl을 포함하고 있는 고체배지에서 PCBs를 분해할 수 있는 Pseudomonas sp. P2 균주를 분해하였다. PCBs의 용해도를 높이기 위해 사용된 유화제 alkyl aryl ethoxylated phosphate가 200 mg/L에서는 Pseudomonas sp. P2 균주의 성장에 영향을 미치지 않았다. 1000 mg/L의 Biphenyl과 PCBs에 Pseudomonas sp. P2를 접종하여 160시간 배양후에 Biphenyl과 PCBs의 분해가 각각 97.5%, 58.0%였다. Biphenyl 1000 mg/L에서 최대성장율($\mu_{max}$)은 0.34 $day^{-1}$, 0.26 였다. 따라서 염소가 결합되지 않은 Biphenyl는 염소가 결합된 PCBs보다 분해가 빠르게 진행되었다. 또한 Pseudomonas sp. P2는 Biphenyl과 PCBs의 분해로 부터 유도된 황색의 분해대사산물을 확인하였다. 본 연구에서는 Pseudomonas sp. P2 균주가 절연유 내의 PCBs를 분해할 수 있다는 것을 확인하였다.

• KSBB Journal
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• v.4 no.2
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• pp.65-68
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• 1989

In order to determin the optimum extraction condition for protein in a Chrysalis of Silk Worm, Temperature, Time, and pH variation were conducted in the extraction. N.S.I. and nitrogen contents of the extracts in this condition were identified by Kjeldaha method and a C.H.N. corder. The results were as follows; Crude protein and crude fat contents in the extracts were 23.34% and 15.61%, respectively. N.S.I. values with respect to the temperature variation were 6.7% at 6$0^{\circ}C$, 7.94% at 8$0^{\circ}C$ and 8.67% at 10$0^{\circ}C$ in the condition of pH 4, 1 hr. N.S.I. values in pH variation were 7.94% at pH4, 8.99% at pH6, 9.70% at pH8, 10.19% at pH 10 and 12.16% at pH 12 in the condition of 8$0^{\circ}C$, 1hr. N.S.I. values in extraction time variation were 8.67% in 1hr, 9.23% in 2hr and 9.76% in 3hr in the condition of 10$0^{\circ}C$, pH 4. The tendency of N.S.I. variation in this extration condition was reconfirmed also by a C.H.N. corder.

• Bulletin of the Korean Chemical Society
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• v.5 no.6
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• pp.215-218
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• 1984

A series of S-(2,2-diethoxycarbonyl-1-phenylethyl)-L-cysteine derivatives (10a-e) were synthesized from the reaction of $\beta$$\beta$-diethoxycarbonylstyrene with L-cysteine in 1:1 aqueous methanol. Thus, S-(2,2-diethoxycarbonyl-1-phenylethyl)-L-cysteine( 10a), S-[2,2-diethoxycarbonyl-1-(3',4'-methylendioxy)ph enylethyl]-L-cysteine (10b), S-[2,2-diethoxycarbonyl-1-(3',4',5'-trimethoxy)phe nylethyl]-L-cyseine (10c), S-[2,2-diethoxycarbonyl-1-(p-hydroxy)phenylethyl] -L-cysteine (10d), S-[2,2-diethoxycarbonyl-1-(p-methoxy)phenylethyl] -L-cysteine (10e) were obtained in moderate to excellent yields. The structure of the adducts was characterized by analytical and spectral data. The effects of pH upon the product yields were also briefly examined.

• Journal of Korean Neurosurgical Society
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• v.50 no.2
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• pp.75-80
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• 2011

Objective : Leukoaraiosis (LA) has been suggested to be related to the poor outcome or the occurrence of symptomatic intracerebral hemorrhage (sICH) after acute ischemic stroke. We retrospectively investigated the influences of LA on long-term outcome and the occurrence of sICH after thrombolysis in acute ischemic stroke (AIS). Methods : In this study, we recruited 164 patients with AIS and magnetic resonance image (MRI)-detected thrombolysis. The presence and extent of LA were assessed using the Fazekas grading system. The National Institutes of Health Stroke Scale score was used to assess the baseline measure of neurologic severity, and the modified Rankin Scale score assessment was used up to 1 year after thrombolysis. Results : Of 164 subjects, 56 (34.2%) showed LA on MRI. Compared to the 108 patients without LA, the patients with LA were of much older age (p<0.01), had a higher prevalence of hypertension (p<0.01), and had a much poorer outcome at 90 days (p=0.05) and 1 yr (p=0.01) after thrombolysis. There were no significant differences in sICH between patients with and without LA on MRI. In univariate analysis for the occurrence of poor outcome at 90 days after thrombolysis, the size of ischemic lesion on diffusion weighted images (DWI), [odds ratio (OR), 1.03; 95% confidence interval (95% CI), 1.01-1.04; p<0.01], recanalization (OR, 0.03; 95% CI, 0.01-0.10; p<0.01), sICH (OR, 12.2; 95% CI, 1.54-95.8), neurologic severity (OR, 1.17; 95% CI, 1.09-1.25; p<0.01), blood glucose level (OR, 1.01; 95% CI, 1.00-1.02; p=0.03), and the presence of LA on MRI (OR, 2.01; 95% CI, 1.04-3.01; p=0.04) were statistically significant. In multivariate analysis, neurologic severity (OR, 1.14; 95% CI, 1.04-1.24; p<0.01), recanalization (OR, 0.03; 95% CI, 0.01-0.11; p<0.01), lesion size on DWI (OR, 1.02; 95% CI, 1.01-1.03; p=0.02), serum glucose level (OR, 1.01; 95% CI; 1.01-1.02; p=0.03), and the presence of LA on MRI (OR, 3.2; 95% CI, 1.22-8.48; p<0.01) showed statistically significant differences. These trends persisted up to 1 yr after thrombolysis. Conclusion : In this study, we demonstrated that the presence of LA on MRI might be related to poor outcome after use of intravenous tissue plasminogen activator in AIS.

• Journal of the korean academy of Pediatric Dentistry
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• v.30 no.1
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• pp.69-79
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• 2003

The purpose of this present study was to develop a new way of self-curing acrylic resin, using commercially available polyphosphate, Calgon, which is known to be antimicrobial and safe. For the study, polyphosphate(polyP) was blended with acrylic powder and devided into four groups as follows: no polyP(control), 1% polyP, 2% polyP, and 3% polyP. For the experiment, Streptococcus mutans GS5, Streptococcus sobrinus 6715, Streptococcus gordonii G9B and Challis, Porphyromonas gingivalis 2561, and Candida albicans ATCC 90027 were used. Resin specimens in each group were tested in vitro for the purpose of investigating the effect of polyP on the microbial attachment, growth and hydrophobicity of the resin surface. The results were as follows. 1. PolyP added to the acrylic resin decreased attachment of S. mutans GS5, S. sobrinus 6715, S. gordonii G9B. The greater binding inhibition was found in acrylic resin polymerized with polyP at higher concentrations. 2. The addition of polyP to acrylic resin failed to significantly affect the growth of the tested microorganisms. 3. The addition of polyP to acrylic resin seemed to reduce hydrophobicity of the acrylic resin. PolyP in acrylic resin does not seem to exert a direct antibacterial activity, but rather inhibit attachment of oral bacteria, especially mutans streptococci to saliva-coated acrylic resin. The acrylic resin reduces attachment of streptococci may be due to the decreased hydrophobicity caused by polyP added to the resin. PolyP may be included to acrylic resin to inhibit dental caries which often occurs when removable acrylic resin appliance is placed.

• Applied Biological Chemistry
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• v.38 no.5
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• pp.455-462
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• 1995

The new six herbicidal N-[(pyrimidin-2-yl)aminocarbonyl]-2-substituted-6-(1-hydroxy-2-fluoroethyl)benzenesulfonamide derivatives(S) were synthesized and rate constants for the hydrolysis of thier in the range of pH $1.0{\sim}10.0$ have been studied in 15%(v/v) aqueous acetonitrile solution at $45^{\circ}C$. From the basis of the results, pH-effect, solvent effect, ortho-substituent effect, thermodynamic parameters(${\Delta}H^{\neq}$ & ${\Delta}S^{\neq}$), pKa constant(4.80), rate equation, analysis of hydrolysis products(2-(1-hydroxy-2-fluoroethyl)benzenesulfonamide & 4,6-dimethoxyaminopyrimidine), it may be concluded that the general acid catalyzed hydrolysis through $A-S_E2$ mechanism and specific acid catalyzed hydrolysis through A-2 type(or $A_{AC}2$) mechanism proceeds via conjugate acid($SH^+$) and tetrahedral intermediate(I) below pH 8.0, whereas, above pH 9.0, the general base catalyzed hydrolysis by water molecules(B) through $(E_1)_{anion}$ mechanism proceeds via conjugate base(CB). In the range between $pH\;7.0{\sim}pH\;9.0$, these two reactions occur competitively.

• Asian-Australasian Journal of Animal Sciences
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• v.10 no.1
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• pp.35-46
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• 1997

An experiment with growing bulls were conducted to determine the effect of supplementation of a straw (S) with 15% molasses and 3% urea as an intimate mix (UMS) on its dry matter (DM) intake and digestibility (DMD) and reduction of methane ($CH_4$) production from fermentation in vitro of the straw. In the next experiment, the feeding of the UMS was compared with that of the feeding of molasses and urea in meals (DS) or in lick blocks (DSUMB) as supplements to straw. The UMS feeding increased daily intake of straw DM ($89.5 g{\cdot}kgW^{-0.75}$, p < 0.01) and digestible crude protein (DCP 333 g, p < 0.001) and nitrogen (N) balances ($508mg{\cdot}kgW^{-0.75}{\cdot}d^{-1}$, p < 0.01) of the bulls than the feeding of 'S' ($65g{\cdot}kgW^{-0.75}$, 55 g and $8.0mg{\cdot}kgW^{-0.75}{\cdot}d^{-1}$, respectively). It also increased the digestibility of DM ($594g{\cdot}kg^{-1}$, p < 0.05), organic matter (OM, $641g{\cdot}kg^{-1}$, p < 0.05), CP ($619g{\cdot}kg^{-1}$, p < 0.001) and acid detergent fibre (ADF, 773, p < 0.05). The $CH_4$ emitted per g of DOM apparently fermented in the rumen (DOMR) was 91.0 ml in the 'S' and reduced (p < 0.05) to 61.6 ml in the UMS. The feeding of the UMS when compared with that of the DS or DSUMB also gave a higher straw intake (1.77% of live weight, LW, p <0.01) and ADF digestibility ($516g{\cdot}kg^{-1}$, p < 0.05) than the other diets (1.52% or 1.55% LW and 472 or $490g{\cdot}kg^{-1}$, respectively) in association with the increased microbial N yield in the rumen (14.1, 5.62 or $17.0g{\cdot}kg^{-1}$ DOMR, respectively, p < 0.05), daily LW gains (233, 125 or 93 g, respectively, p < 0.05) and feed conversion ratios of the diets (26.0, 56.1, or 57.6 g feed/g LW gain, p > 0.05, respectively). It can be concluded that molasses and urea feeding as an intimate mix with straw (UMS) increased its digestion and intake in association with a reduced methane emissions in the rumen. When compared with that of their feeding in meals or in lick blocks as supplements to straw the UMS gave the highest straw in take and digestion and live weight gains of growing bulls concurring the finding that the UMS system may be the best way of molasses and urea feeding to ruminants fed straws.

• Journal of Korean Academy of Nursing
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• v.4 no.2
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• pp.144-158
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• 1974

This study was undertaken in an attempt to; 1) measure value orientation of nursing activities, 2) identify the relationship between nurse's role perception and patient's expectation, and 3) evaluate the pattern of nursing education and nursing service administration. 203 hospitalized adults and 203 professional nurses from 11 general hospitals in Seoul during the period of July to December 1973 were tested according to questionnaire based on 4 categories of clinical nursing activities, 1) physical care, 2) observation and control, 3) psycho-social care and 4) therapeutic measures. Results of the study are as follows: 1. Nurses were more concerned than patients in the physical care category. Significant difference was revealed by p<.01 level (t=2.800). Mean value score of nurses was average (2.84), and mean value score of patients was relative' y low (2.49). None of the physical care category questionnaire items were over 3.5. 2. Respondents from hospitals of total care system revealed significant difference in the physical care category by P〈.025 level. (t=2.242). Mean value score of both group were average level (nurse 2.90, patient 2.53), nurses showed higher concern. 3. Difference between nurse's role perception and patient's expectation in observation and control category was revealed non- significance by p〉0.05. level (t=1.238). Mean value score of both group revealed relatively high (nurse=3.44, patient 3.19). 4. Difference between nurse's role perception and patient〉expectation in psycho-social cart category revealed non- significance by p〉.05 level (t=0.758), Mean value score of both group revealed average level (nurse =2.71, patient =2.53), 5. Non- significant difference was noted between B. S. N. and diploma nurse's role perception in the psycho- social care category by p> 0.1 level (t=0.316). 4. Difference between nurse's role perception and patient's expectation in the rapeutic me assures category revealed non- significance by p〉0.05 level) t=0.503). Horses showed high concern by mean value score 3.56 level and patients relatively high by 3.41. All items of this category revealed very high or relatively high value score.

• Asian-Australasian Journal of Animal Sciences
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• v.24 no.9
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• pp.1243-1248
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• 2011

The aim of this study was to determine the usefulness of vegetable wastes as silage for ruminants. Varying amounts of wheat straw (WS), wheat bran (WB) and salt (S) were combined with minced vegetable wastes (VW) during ensilage. Seven different ingredient combinations were investigated viz: 100% VW (Group I, control), 90% VW+9% WS+1% S (Group II), 80% VW+15% WS+4% WB+1% S (Group III), 70% VW+20% WS+9% WB+1% S (Group IV), 90% VW+9% WB+1% S (Group V), 80% VW+15% WB+4% WS+1% S (Group VI) and 70% VW+20% WB+9% WS+1% S (Group VII). The inclusion of straw and bran increased (p<0.01) the DM content of silage. The highest contents of the pure silage were CP (p<0.001), EE (p<0.01) and NFE (p<0.05). NDF contents of VW silage and group V were significantly lower and especially the VW silage was found to have the lowest ADL content (p<0.01). The in vitro ME values of VW silage and bran added silage were higher than other groups (p<0.01). pH, lactic acid and acetic acid values of silage groups were changed between 4.09-4.20, 2.43-3.46% and 0.60-0.86%, respectively. In conclusion, different mixtures of VW have a high ensilage capacity and can serve as an alternative roughage source for ruminants. The addition of 9% bran significantly improved the silage in view of both dry matter content and nutritive value.