• Microbiology and Biotechnology Letters
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• v.26 no.1
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• pp.68-75
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• 1998

The overproduction and high level secretion of Glucose Oxidase (GOD) from A. niger in S. cerevisiae was carried out by cloning GOD gene. For this purpose, using two different strong promoters (ADH1 promoter, GAL10 promoter) and signal sequences (${alpha}$-MF signal sequence of S. cerevisiae and ${alpha}$-amylase signal sequence of A. oryzae) and GAL7- and GOD terminator, four expression vectors were constructed. All the expression vectors were transformed in S. cerevisiae 2805 using auxotroph method. By the flask culture, transformants of pGAL expression vector series containing GAL 10 promotor showed much higher GOD productivity than transformants of pADH expression vector series containing ADH1 promoter Transformants of pGALGO2 containing GAL10 promotor and ${alpha}$-amylase signal sequence has shown the best productivity of GOD ($GOD_{total}$: 10.3 unit/mL, $GOD_{ex}$: 8.7 unit/mL) at 115 hr. This value was three fold higher than that of pGALGO1 containing GAL 10 promotor and ${alpha}$-MF signal sequence, even if the same promotor was involved. Through the ${alpha}$-amylase signal sequence of A. oryzae, GOD was secreted much more than the case of ${alpha}$-MF signal sequence from S. cerevisiae. These results suggest that signal sequence may play a important roles in not only the secretion but also the overproduction of foreign protein. Secretion rate of GOD in pGALGO1 and pGALGO2 was 89% and 84%, respectively, Because of the overglycosylation in S. cerevisiae the molecular weight of recombinant GOD in S. cerevisiae was much larger (250 kDa) than that of nature GOD in A. niger (170 kDa).

• Journal of Life Science
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• v.18 no.1
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• pp.136-142
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• 2008

A recombinant Pichia pastoris carrying double expression cassette of Rhodotorula glutinis epoxide hydrolase(RgEH) gene was developed and used for preparing enantiopure (S)-styrene oxide from racemic mixture of styrene oxide. BglII restriction site of original RgEH gene (pPICZ B/RgEH #2) of previous report was mutated using PCR technique for the construction of double expression cassette containing promoter ($P_{AOX1}$), EH gene and transcription terminator ($TT_{AOX1}$) in pPICZ C vector. Double expression cassette with RgEH was inserted into the chromosomal DNA of P. pastoris. $V_{max}$ ($2.2{\mu}mol\;min^{-1}mg\;dcw^{-1}$) on (R)-styrene oxide of P. pastoris with double expression cassette was about 6-fold higher than that ($0.4{\mu}mol\;min^{-1}mg\;dcw^{-1}$) of P. pastoris with single expression cassette. For the determination of the optimal condition, the effects of detergent and temperature on the enantioselective hydrolytic activity and yield of the enantiomer were investigated. When the reaction was performed at $10^{\circ}C$ for 10 min in the presence of 0.5% Tween 20, enantiopure (S)-styrene oxide with 99.9% ee was obtained as the yield 43.4 % from 20 mM racemic sustrate.

• KSBB Journal
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• v.20 no.1 s.90
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• pp.46-49
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• 2005

Curdlan, a natural $\beta-1,3-glucan,$ has been studied as drug carrier due to its unique properties including its thermal gelling characters. In this study, curdlan was chemically acetylated for pH-sensitive drug delivery. Curdlan acetate microspheres(CAMs) were prepared by the solvent evaporation method. The size of the CAMs was below $200{\mu}m.$ The drug loading efficiency of microspheres was approximately $58.44\%$. In the swelling test, the CAMs showed pH-sensitive behavior. The swelling capacity of microspheres at pH 7.4 was much greater than at pH 1.4. Also, release rate of indomethacin(IND) at pH 7.4 from the CAMs was faster than that at pH 1.4. Therefore the CAMs have potential for the controlled release of IND over an extended period of time.

• Bulletin of the Korean Mathematical Society
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• v.56 no.6
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• pp.1643-1653
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• 2019

This paper shows that if $1<p<{\infty}$, ${\alpha}{\geq}-n-2$, ${\alpha}>-1-{\frac{p}{2}}$ and f is holomorphic on the unit ball ${\mathbb{B}}_n$, then $${\int_{{\mathbb{B}}_n}}{\mid}Rf(z){\mid}^p(1-{\mid}z{\mid}^2)^{p+{\alpha}}dv_{\alpha}(z)<{\infty}$$ if and only if $${\int_{{\mathbb{B}}_n}}{\int_{{\mathbb{B}}_n}}{\frac{{\mid}f(z)-F({\omega}){\mid}^p}{{\mid}1-(z,{\omega}){\mid}^{n+1+s+t-{\alpha}}}}(1-{\mid}{\omega}{\mid}^2)^s(1-{\mid}z{\mid}^2)^tdv(z)dv({\omega})<{\infty}$$ where s, t > -1 with $min(s,t)>{\alpha}$.

• Asian-Australasian Journal of Animal Sciences
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• v.26 no.8
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• pp.1057-1064
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• 2013

p70 ribosomal S6 kinase (p70S6K) can integrate nutrient and growth factor signals to promote cell growth and survival. We report our molecular characterization of the complementary DNA (cDNA) that encodes the goat p70S6K gene 40S ribosomal S6 kinase 1 (S6K1) (GenBank accession GU144017) and its 3' noncoding sequence in Inner Mongolia Cashmere goats (Capra hircus). Goat S6K1 cDNA was 2,272 bp and include an open reading frame (ORF) of 1,578 bp, corresponding to a polypeptide of 525 amino acids, and a 694-residue 3' noncoding sequence with a polyadenylation signal at nucleotides 2,218 to 2,223. The relative abundance of S6K1 mRNA was measured by real-time PCR in 6 tissues, and p70S6K expression was examined by immunohistochemistry in heart and testis. The phosphorylation of p70S6K is regulated by mitogen-activated protein kinase (MAPK) signaling in fetal fibroblasts.

• Journal of the Korean Mathematical Society
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• v.57 no.3
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• pp.747-775
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• 2020

In this work we investigate the nonlocal elliptic equation with critical Hardy-Sobolev exponents as follows, $$(P)\;\{(-{\Delta}_p)^su={\lambda}{\mid}u{\mid}^{q-2}u+{\frac{{\mid}u{\mid}^{p{^*_s}(t)-2}u}{{\mid}x{\mid}^t}}{\hspace{10}}in\;{\Omega},\\u=0{\hspace{217}}in\;{\mathbb{R}}^N{\backslash}{\Omega},$$ where Ω ⊂ ℝ^N is an open bounded domain with Lipschitz boundary, 0 < s < 1, λ > 0 is a parameter, 0 < t < sp < N, 1 < q < p < p^∗_s where $p^*_s={\frac{N_p}{N-sp}}$, $p^*_s(t)={\frac{p(N-t)}{N-sp}}$, are the fractional critical Sobolev and Hardy-Sobolev exponents respectively. The fractional p-laplacian (-∆_p)^su with s ∈ (0, 1) is the nonlinear nonlocal operator defined on smooth functions by $\displaystyle(-{\Delta}_p)^su(x)=2{\lim_{{\epsilon}{\searrow}0}}\int{_{{\mathbb{R}}^N{\backslash}{B_{\epsilon}}}}\;\frac{{\mid}u(x)-u(y){\mid}^{p-2}(u(x)-u(y))}{{\mid}x-y{\mid}^{N+ps}}dy$, x ∈ ℝ^N. The main goal of this work is to show how the usual variational methods and some analysis techniques can be extended to deal with nonlocal problems involving Sobolev and Hardy nonlinearities. We also prove that for some α ∈ (0, 1), the weak solution to the problem (P) is in C^1,α(${\bar{\Omega}}$).

• Journal of the Korean earth science society
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• v.22 no.6
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• pp.500-511
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• 2001

For the southeastern Korea aound the Yangsan fault we measured Q$_P^{-1}$ and Q$_S^{-1}$ simultaneously by using the extended coda-normalization method for seismograms registered at 9 stations deployed by KIGAM. We analyzed 707 seismograms of local earthquakes that occurred between December 1994 and February 2000. From seismograms, bandpass filtered traces were made by applying Butterworth filter with frequency-bands of 1${\sim}$2, 2${\sim}$4, 4${\sim}$8, 8${\sim}$16 and 16${\sim}$32 Hz. Estimated Q$_P^{-1}$ and Q$_S^{-1}$ values decrease from (7${\pm}$2)${\times}$10$^{-3}$ and (5${\pm}$4)${\times}$10$^{-4}$ at 1.5 Hz to (5${\pm}$4)${\times}$10$^{-3}$ and (5${\pm}$2)${\times}$10$^{-4}$ at 24 Hz, respectively. By fitting a power-law frequency dependent to estimated values over the whole stations, we obtained 0.009 (${\pm}$0.003)f$^{-1.05({\pm}0.14)$ for Q$_P^{-1}$ and 0.004 (${\pm}$0.001)f$^{-0.75({\pm}0.14)$) for Q$_S^{-1}$, where f is frequency in Hz.

• Journal of Sasang Constitutional Medicine
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• v.1 no.1
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• pp.171-181
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• 1989

We have studied some effects of the SOYANGIN-HYEONGBANGPAEDOK-SAN (S.H.P.). Several empirical remarks, depending on the different treatments, are investigated through this study as follow; 1. The Analgesic effects of S.H.P. are remarked by Acetic acid method in mice, 100 and 300mg/kg (Sample II, III), 2. The Antipyretic effect of S.H.P. are remarked by yeast method in rats, 100 and 300mg/kg (Sample II, III). 3. The inhibitory effects of S.H.P. on strychnine induced convulsion in mice is remarked. However the inhibitory effects of S.H.P. on picrotoxin induced convulsion in mice is not observed. 4. The effects of S.H.P. on muscle relaxation in mice, using the Rota-Rod test, is remarked on 1,2,3 hours by 300mg/kg (Sample III).

• Journal of the Korean Mathematical Society
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• v.48 no.2
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• pp.397-420
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• 2011

In this paper we study the structure of closed weakly dense ideals in Privalov spaces $N^p$ (1 < p < $\infty$) of holomorphic functions on the disk $\mathbb{D}$ : |z| < 1. The space $N^p$ with the topology given by Stoll's metric [21] becomes an F-algebra. N. Mochizuki [16] proved that a closed ideal in $N^p$ is a principal ideal generated by an inner function. Consequently, a closed subspace E of $N^p$ is invariant under multiplication by z if and only if it has the form $IN^p$ for some inner function I. We prove that if $\cal{M}$ is a closed ideal in $N^p$ that is dense in the weak topology of $N^p$, then $\cal{M}$ is generated by a singular inner function. On the other hand, if $S_{\mu}$ is a singular inner function whose associated singular measure $\mu$ has the modulus of continuity $O(t^{(p-1)/p})$, then we prove that the ideal $S_{\mu}N^p$ is weakly dense in $N^p$. Consequently, for such singular inner function $S_{\mu}$, the quotient space $N^p/S_{\mu}N^p$ is an F-space with trivial dual, and hence $N^p$ does not have the separation property.

• Korean Journal of Microbiology
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• v.28 no.1
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• pp.35-40
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• 1990

The restriction clevage map of multi-copy recombinant plasmid, pJY712(8.1kb), carrying the thiostrepton resistance gene(tsr) was determined. pJY712 had a broad host range in Streptomyces and contained single BglII site for cloning purpose. The plasmid showed the phenomenon of lethal zygosis ($Ltz^{+}$). Transformation frequency of pJY712 was $5.0\times 10^{4}$ transformants per ug plasmid DNA (TFU) in S. lividans. Plasmid pJY713 was constructed by inserting the tyrosinase gene(mel) into the BclI site of pJY712. Recombinant plasmid pJY714 carrying the mel gene was constructed by in vitro deletion of a segment (1.9kb BglII-BclI fragment) from pJY713.