• 구강회복응용과학지
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• 제37권3호
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• pp.123-129
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• 2021

목적: 본 연구의 목적은 효모형 또는 균사형 Candida albicans에 대한 표고버섯 추출물의항진균효과를 살펴보기 위함이다. 연구 재료 및 방법: 표고버섯 추출물은 표고버섯을 물 또는 에탄올에 닮근 후, 상층액을 건조시켜서 얻었다. 효모형 C. albicans에 대한 추출물의 항진균활성은 마이크로플레이트를 이용한 감수성 시험을 이용하여 조사되었다. C. albicans 생물막을 CO₂ 배양기에서 Ham's F-12 배지를 이용하여 12-well 플레이트에 형성시키고 에탄올 추출물로 처리하였다. 또한 C. albicans 생물막을 의치상용 레진 디스크에 형성시키고 의치세정제를 에탄올 추출물이 포함 또는 포함되지 않은 조건에서 처리하였다. 두 조건의 항바이오필름 효과 시험에서의 바이오필름내 살아있는 C. albicans를 조사하기 위해서 한천고체배지에 접종한 후 집락 형성 단위(CFU) 값을 측정하였다. 결과: 효모형 C. albicans에 대해서 표고버섯으로부터 물 추출물보다 에탄올 추출물이 강한 항진균력을 보였다. 에탄올 추출물은 균사형 C. albicans 바이오필름에 대해서도 유의적인 항진균력을 보였다(P < 0.05). 또한 에탄올 추출물은 의치세정제와 의치에 형성된 C. albicans 바이오필름에 대한 항진균력에 대해서 동반상승효과를 보였다(P < 0.05). 결론: 표고버섯 에탄올 추출물은 구강 칸디다증 예방뿐만 아니라 의치관련 구내염에 대해서 예방할 수 있는 후보물질로 사료된다.

• Natural Product Sciences
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• 제9권1호
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• pp.10-12
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• 2003

In the present study, dikamali resin (obtained from the leaf buds and the young shoots of Gardenia gummifera Linn.) was extracted with diethyl ether and the extract was vacuum dried. Qualitative tests confirmed the presence of flavonoids and free phenolic compounds in the extract. The antioxidant property (qualitative) of the extract was performed by TLC method $({\beta}-carotene-linoleate method)$. The $LD_{50}$ of the extract was found to be 2227 mg/kg by Karber's arithmetic method. The extract was screened for analgesic, anti-inflammatory, antipyretic (100, 200 and 400 mg/kg) and anthelmintic (0.1,0.2 and 0.5%w/v) activities by standard methods. The extract exhibited antioxidant property and prevented oxidation of ${\beta}-carotene$. The extract exhibited significant graded dose response for analgesic, anti-inflammatory, antipyretic and anthelmintic activities. The extract caused the death of earthworms in all experimental concentration whereas the standard drug (piperazine) only effected paralysis. The present study proved the claims of dikamali resin mentioned in the Indian system of medicine.

• 한국식품영양학회지
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• 제17권1호
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• pp.8-17
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• 2004

Chlorella 추출물을 첨가한 yoghurt를 개발하기 위한 기초 연구로 chlorella 추출물의 첨가가 yoghurt starter의 산 생성 및 증식에 미치는 영향을 조사하였다. Str. themophilus, Lac. acidophilus, Lac. casei, Lac. bulgaricus를 단독 균주 및 혼합 균주로 접종하여 배양하면서 생균 수와 pH 및 적정산도를 측정하였다. 단독 균주의 경우 chlorella 추출물 분말의 첨가 농도(0.5%, 1.0%, 2.0%, 3.0%)를 달리 하였을 때 Str. thermophilus, Lac. casei와 Lac. bulgaricus는 chlorella 추출물 분말 0.5% 첨가 시 9시간 배양에서, 그리고 Lac. acidophilus는 1.0% 첨가 시 15시간 배양에서 최대 균수를 나타내었으며, 이들 중 Lac. casei가 0.5% 첨가 시 배양 9시간에 3.63${\times}$$10^{9}$ CFU/mL로 가장 높은 생균 수를 보였다. 또한 이들 유산균의 산 생성도 chlorella 추출물 분말을 0.5% 첨가하였을 때 가장 많이 증가하였다. 혼합균주의 경우 chlorella 추출물 분말을 0.25%, 0.5%, 1.0%, 2.0%로 첨가하였을 때 저농도(0.25∼0.5%)의 chlorella 추출물 분말 첨가에 의해 모든 유산균 혼합 균주의 증식이 촉진되었고, pH가 크게 하락하였으며 적정산도가 크게 상승하였다. Str. thermophilus와 Lac. casei를 혼합 배양하였을 때 chlorella 추출물 분말 0.25% 첨가 시 12시간 배양 후 1.13${\times}$$10^{8}$ CFU/mL로 생균수 가 가장 높아 4.12 의 log cycle 증가를 나타내었다. 따라서 chlorella 추출물 첨가 yoghurt 제조 시 chlorella 추출물 분말을 0.25% 첨가하여 혼합 starter로 Str. thermophilus와 Lac. casei 혼합균주를 사용하는 것이 이들 유산균의 생육이 촉진되어 가장 적합한 것으로 나타났다.

• Journal of Microbiology
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• 제44권5호
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• pp.502-507
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• 2006

In a previous study, an extract of Clavicorona pyxidata DGUM 29005 mycelia demonstrated an inhibitory effect against enzyme-associated perceptual disorders. We have attempted to determine whether this mycelial extract is also capable of inhibiting the activities of acetylcholinesterase (AChE) and ${\beta}$-secretase (BACE) activity. Butanol, ethanol, and water extracts of C. pyxidata DGUM 29005 mycelia were shown to inhibit AChE activity by 99.3%, 93.7%, and 91.7%, respectively. The inhibitory value of the butanol extract was more profound than that of tacrine (95.4%). The ethanol extract also exerted an inhibitory effect against BACE activity; this fraction may harbor the potential for development into a pharmocotherapeutic modality for the treatment of Alzheimer's disease (AD) patients. Rat pheochromocytoma PC12 cells in culture were not determined to be susceptible to the cytotoxic activity evidenced by the mycelial extract. The ethanol extract inhibited endogenous AChE activity in PC12 cellular homogenates, with an $IC_{50}\;of\;67.5{\mu}g/ml$, after incubation with intact cells, and also inhibited BACE activity in a dose-dependent fashion. These results suggest that the C. pyxidata mycelial extract has the potential to enhance cholinergic function and, therefore, may perform a function in the amelioration of the cholinergic deficit observed in cases of AD, as well as other types of age-associated memory impairment.

• 동의생리병리학회지
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• 제20권2호
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• pp.394-403
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• 2006

This research investigated the effect of the CMT and MCMT on Alzheimer's disease. The effects of the CMT and MCMT extract on expression of proinflammatory cytokine($IL-1{\beta}$, IL-6, $TNF-{\alpha}$) in the THP-1 cell; amyloid precursor proteins(APP), acetylcholinesterase(AChE) mRNA of PC-12 cells treated with CT105; the AChE activity and the APP production of PC-12 cell lysate treated with CT105 were investigated. The CMT and MCMT extract suppressed overexpression of $IL-1{\beta}$, IL-6, $TNF-{\alpha}$ in the THP-1 cell treated by LPS; the expression of APP, AChE mRNA in PC-12 cells treated with CT105; the AChE activity and the production of APP in PC-12 cell lysate treated with CT105 significantly. This study suggest that CMT and MCMT may be effective for the prevention and treatment of Alzheimer's disease.

• 동의생리병리학회지
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• 제20권1호
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• pp.138-148
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• 2006

This research investigated the effect of the CMT and SCMT on Alzheimer's disease. The effects of the CMT and SCMT extract on expression of proinflammatory cytokine(IL-$1{\beta}$, IL-6, TNF-$\alpha$) in the THP-1 cell; amyloid precursor proteins(APP), acetylcholinesterase(AChE) mRNA of PC-12 cells treated with CT105; the AChE activity and the APP production of PC-12 cell lysate treated with CT105 were investigated. The CMT and SCMT extract suppressed overexpression of IL-$1{\beta}$, IL-6, TNF-$\alpha$ in the THP-1 cell treated dy LPS; the expression of APP, AChE mRNA in PC-12 cells treated with CT105; the AChE activity and the production of APP in PC-12 cell Iysate treated with CT105 significantly. This study suggest that CMT and SCMT may be effective for the prevention and treatment of Alzheimer's disease.

• 대한본초학회지
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• 제30권1호
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• pp.59-65
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• 2015

Objectives : The purpose of this study was to observe the effects of Polygalae Radix(PR) on 4-HNE-induced apoptosis in PC-12 cell. Methods : A MTT assay was conducted to observe the cytotoxicity of Polygalae Radix on the cell viability and the cytoprotective effect of Polygalae Radix against 4-HNE that causes oxidative stress-induced cytotoxicity, and then a western blot was conducted to observe the expression of $TNF-{\alpha}$, caspase-3, Bax and Bcl-2 protein that are important factors involved with apoptosis signaling pathway. Results : The Polygalae Radix water extract $25{\mu}g$, $50{\mu}g$, $100{\mu}g$ and $200{\mu}g/mL$ had no cytotoxicity on the PC-12 cell. The Polygalae Radix water extract $25{\mu}g$, $50{\mu}g$ and $100{\mu}g/mL$ had the cytoprotective effect against 4-HNE that causes cytotoxicity on the PC-12 cell. The Polygalae Radix water extract $50{\mu}g/mL$ significantly suppressed the increase in $TNF-{\alpha}$ protein expression in PC-12 cell. The Polygalae Radix water extract $25{\mu}g$ and $50{\mu}g/mL$ significantly suppressed the increase in caspase-3 protein expression in PC-12 cell. The Polygalae Radix water extract $25{\mu}g$, $50{\mu}g$ and $100{\mu}g/mL$ suppressed the increase in Bax protein expression in PC-12 cell but had no significance. The Polygalae Radix water extract $25{\mu}g$ and $100{\mu}g/mL$ significantly prevented the decrease in Bcl-2 protein expression in PC-12 cell, Conclusions : These results suggest that the Polygalae Radix water extract is effective in inhibiting apoptosis.

• 농업과학연구
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• 제21권2호
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• pp.111-121
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• 1994

탈지유배지(脫脂乳培地)에 인삼(人蔘)extract를 0.3~2.4%를 첨가(添加)하고 yoghurt starter의 기본균종(基本菌種)인 Str. thermophilus, L. bulgaricus 및 2균종(菌種) 혼합균주(混合菌株)를 배양(培養), 배양시간(培養時間)에 따른 산생성(酸生成) 및 균증식(菌增殖) 상태(狀態)를 시험(試驗)한 결과(結果)는 다음과 같다. 1. 인삼(人蔘)extract 첨가(添加) 탈지유배지(脫脂乳培地)에 Str. thermophilus 및 L. bulgaricus를 각각(各各) 배양(培養)할 때 12시간(時間)에 Str. thermophilus control구(區)는 1.11%, L. bulgaricus control구(區)는 1.01%의 산도(酸度)를 나타내었으나 인삼(人蔘)extract 첨가구(添加區)에 있어서는 첨가량(添加量)의 증가(增加)에 따라 Str. thermophilus는 1.08~0.61%, L. bulgaricus는 0.99~0.49%의 산도(酸度)를 나타내 산생성(酸生成)이 억제되는 현상(現象)을 나타내었다. 2. 인삼(人蔘)extract 첨가(添加) 탈지유배지(脫脂乳培地)에 Str. thermophilus 및 L. bulgaricus를 배양(培養)할 때 유산균수(乳酸菌數)의 증가(增加) 상태(狀態)는 12시간(時間)에 Str. thermophilus control구(區)가 $5.2{\times}10^8/m{\ell}$, L. bulgaricus control구(區)는 $3.2{\times}10^8/m{\ell}$이었으며 인삼(人蔘)extract첨가구(添加區)에 있어서는 Str. thermophilus가 $3.6{\times}10^8/m{\ell}{\sim}1.3{\times}10^8/m{\ell}$, L. bulgaricus에 있어서는 $2.9{\times}10^8/m{\ell}{\sim}1.4{\times}10^8/m{\ell}$로서 인삼(人蔘)extract의 첨가량(添加量) 증가(增加)에 따라 균증식상태(菌增殖狀態)가 둔화되는 현상(現象)을 나타내었다. 3. Str. thermophilus 및 L. bulgaricus의 혼합균주(混合菌株)를 인삼(人蔘)extract의 첨가(添加) 탈지유배지(脫脂乳培地)에 배양(培養)할 때 control구(區)와 인삼(人蔘)extract 첨가구간(添加區間)에 산생성(酸生成) 및 균증식(菌增殖) 상태(狀態)에 있어 큰 차이(差異)를 나타내지 않았으며 인삼(人蔘)extract 첨가구(添加區)에 딸사서는 오히려 증가(增加)하였다.

• 한국식품조리과학회지
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• 제6권3호통권12호
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• pp.17-24
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• 1990

1. The major components of Omija were found as moisture (16.12%) crude fat (10.5%) crude protein (8.66%) crude ash (3.47%) reducing sugar (5.32%). 2. Omija jelly receipe were summarized as follow; (1) 5% of gelatin concentration was shown to be the most desirable. (2) 15% of sugar concentration was shown to be the most desirable. (3) 12, 15, 18 hrs. of extract time was not significantly different in Omija extract. (4) It was the most desirable for 30g of Omija to boil during 30 minutes in boiling Omija extract. 3. Result of Omija jelly by Instron that Hardness, Adhesiveness, Gumminess tend to increase as the addition level of gelatin concentration increased but Cohesiveness was changed irregularly. 4. Hardness Adhesiveness tend to increase as the addition level of increase as the addition level of sugar concentration increased but Cohesiveness Gumminess was not significant difference. 5. Hardness, Adhesiveness tend to increase as the addition level of extract time increased, but Cohesiveness, Gumminess was not significant difference. 6. Color value of Omija jelly (gelatin 5%, sugar 15%, 18hr. extract) was shown $x=7.94{\pm}0.047,\;y=4.24{\pm}0.047,\;z=6.34{\pm}0.067$ in Omija extract and Omija jelly (gelatin 5%, sugar 15%, Omija 50g, 30 minutes boiling) was shown $x=7.76{\pm}0.038,\;y=3.88{\pm}0.02,\;z=6.12{\pm}0.021$ in boiling Omija extract. 7. Appearance of Omija jelly had significant relationship with Cohesiveness by gelatin concentration and texture of Omija jelly had significant relationship with Adhesiveness by gelatin concentration. 8. Appearance of Omija jelly had significant relationship with Cohesiveness and texture of Omija jelly had significant relationship with Gumminess by boiling Omija extract.

• 한국염색가공학회지
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• 제12권4호
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• pp.239-247
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• 2000

The coloring matter was extracted from the heartwood of Amur cork tree by distilled water. Change of UV-Visible spectra of coloring matter solution by extraction condition and stability for irradiation were determined, and the effect of repeated dyeing with condition of dyebath and mordanting method on shade depth and lightfastness were also investigated. The results are as follows : 1) Absorbance of Amur cork tree extract increased with the lapse of extraction time. λmax of color solution extracted from Amur cork tree was found at 420, 333, and 262nm. 2) Absorbance of Amur cork tree extract decreased remarkably after 2hr irradiation. 3) The K/S of silk fabrics increased with the increase of dyeing temperature, time, amounts of Amur cork tree for extraction, and pH of color solution. 4) K/S of silk fabrics dyed by repeated dyeing method was affected by pH and concentration of color solution. 5) Lightfastness of silk twice dyed with Amur cork tree extract after pre-mordanted by 8%(o.w.f) chromium acetate was moderately improved.