• Title/Summary/Keyword: S100A14

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Development of Sandwich ELISA for the Detection of Shrimp in Processed Foods (가공식품 중 새우의 검출을 위한 샌드위치 ELISA의 개발)

  • Do, Jeong-Ryong;Back, Su-Yeon;Shon, Dong-Hwa
    • Korean Journal of Food Science and Technology
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    • v.46 no.5
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    • pp.538-543
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    • 2014
  • A sandwich enzyme-linked immunosorbent assay method (sELISA) for detecting the presence of shrimp in processed foods was developed using rabbit polyclonal antibodies against tropomyosin produced by black tiger prawns (shrimp). Based on the standard curve derived using this method, the detection range of shrimp was determined to be $1-100{\mu}g/mL$. The cross-reactivity of these antibodies toward black tiger prawns, fleshy prawns, cocktail prawns, lobster, and blue crab was 100, 73, 155, 18, and 0%, respectively. When shrimp was heated for 10 min, the mean assay recovery of tropomyosin was 121-221% at $70-100^{\circ}C$ and 7.8% at $121^{\circ}C$. When shrimp was added to cream soup, weaning food, sausage, fish paste, and sauce, the mean assay recovery was 397, 639, 168, 234, and 0%, respectively. In sample tests involving 14 commercial items, the coincidence ratio of assay results and reference was 79%.

Characteristics of time-resolved optically stimulated luminescence (OSL) on Paleolithic paleosol quartz (구석기 고토양층 석영의 시간분해 광자극 냉광 특성 연구)

  • Hong, Duk-Geun;Kim, Ki-Bum;Kim, Myung-Jin
    • Analytical Science and Technology
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    • v.31 no.1
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    • pp.7-13
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    • 2018
  • In this study, we measured the time-resolved optically stimulated luminescence (TR-OSL) of Paleolithic paleosol quartz and evaluated its lifetime. Considering the lifetime dependence on the preheating applied after irradiation, the radiation exposure, and the optical pulse stimulation, we found that the optimum measurement condition for determining the lifetime of paleosol quartz was the optical pulse stimulation at a dwelling time of $250{\mu}s$, pulse width of $10{\mu}s$, and sweep number of 100,000, without preheating after 100 Gy of irradiation. Based on the dependence of the lifetime on the reading temperature, the thermal quenching activation energy ${\Delta}E$ and thermal assistance activation energy $E_a$ were evaluated as $0.60{\pm}0.14eV$ and $0.053{\pm}0.029eV$, respectively. These values were in good agreement with those reported in the literature. Therefore, we concluded that the resulting kinetic parameters for paleosol quartz are quite reliable.

Fine Needle Aspiration Cytopathology of Pediatric Lympha denopathy among Sudanese Children

  • Ahmed, Hussain Gadelkarim;Elmubasher, Maysa Badawi;Salih, Rashid Awad Abdallah;Elhussein, Gamal Eldin Mohamed Osman;Ashankyty, Ibraheem Mohmmed Alamin
    • Asian Pacific Journal of Cancer Prevention
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    • v.14 no.7
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    • pp.4359-4363
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    • 2013
  • Background: Pediatric lymphadenopathy is a challenging medical situation for the child patient, the parents, and the physician. Although the bulk of masses will be benign the fear of malignancy is omnipresent. Therefore, the objective of this study was to identify the common cytopathological patterns of lymphadenopathy among Sudanese children. Methods: One hundred pediatric patients presenting with peripheral lymphadenopathy were included in the study, their ages ranging from 2 to 14 years, with a mean age of 7 years. Demographic characteristics, clinical manifestations and FNA materials were prospectively obtained. Results: FNA was performed in 100 cases (100%). There were no technical complications. All cases confirmed adequacy of specimen. Overall, FNA demonstrated 90 (90%) benign lesions and 10 (10%) malignant diagnosis. The benign lesions were reactive lymphoid hyperplasia (n=64), followed by benign granulomatous disease (n=26). Of the 10 cases diagnosed with malignancy, 7 (7%) were cases of non-Hodgkin`s lymphoma and the remaining 3 (3%) were Hodgkin's lymphomas. Conclusion: Pediatric lymphadenopathy is common in Sudan. CLA is the common frequent site. Lymphoma represents a major challenge in this setting.

Study 1 of The 100 Best Chinese Films for Storytelling Content Development of Korea and China's Collaborative Film Production -Focused on Films in 1930's- (한중합작 영화의 스토리텔링 콘텐츠개발을 위한 중국 100대 영화연구 1 -1930년대 작품을 중심으로-)

  • Han, Dal-Ho
    • The Journal of the Korea Contents Association
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    • v.14 no.11
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    • pp.111-124
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    • 2014
  • This dissertation is to seek new valuable possibility of the 100 best Chinese films on storytelling in Chinese films as considering about content development of Korea and China's Collaborative film production and extension, that being magnified as a new content for variety of Korean film production. The moment to the Korean wave is getting cold in Japan, Korean wave is attempt to receive attention in new field through China by various image field with Korean drama's appeal. However, film content fall short of our expectation than media content, even if supporting much various attempts with Korea and China's Collaborative film production content. Specially, there are not satisfied films for Korea and China both counties's audience, although Korea and China's Collaborative film production has been produced steadily. The writer studies on stories of Chinese essential 100 films for searching deeply this problem. Among them, in selected 'The 100 Best Chinese Films' by China in 2005, films in 1930's are selected as the first step to study, and this dissertation searches to communicate storytelling's meaning in Chinese films through that time image. This studies what is the point of selected story by China, and there are what kind of worries. This dissertation considers that focused on characters, event and background. Chinese film in 1930's represented life in times through process of play development that is connected with poverty, reality of society, death, tragedy and tragicomedy.

Antioxidant, α-amylase and α-glucosidase inhibitory ability effects of sesame meal ethanol extract (참깨박 에탄올 추출물의 항산화 및 α-amylase및 α-glucosidase 저해 활성)

  • Wu, Ying Jin Zhu;Kim, Myung Hyun;Han, Young Sil
    • Journal of Applied Biological Chemistry
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    • v.65 no.3
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    • pp.189-194
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    • 2022
  • In this study, a sesame meal was used in order to analyze the proximate composition and mineral contents. The sesame seed meal, pressed from roasted Sesame seed, contains various polyphenols. The defatted sesame meal was extracted using 70% ethanol, and its antioxidant activity and antidiabetic effects were evaluated. Proximate composition of sesame meal was showed that moisture 6.51%, carbohydrate 16.22%, crud protein 46.30%, ash 9.88%, crude fat 21.09%. Mineral contents were K 1128.08 mg/100 g, Ca 1356.27 mg/100 g, Fe 12.29 mg/100 g, P 2022.14 mg/100 g, Cu 2.08 mg/100 g, Mg 643.40 mg/100 g, Na 7.29 mg/100 g. The results showed the sesame meal of 70% ethanol extract had higher polyphenol content (184.98 mg GAE/g) and flavonoid content (27.63 mg QE/g). The 2,2-diphenyl-1-picrylhydra-zyl and 2,2'-aziono-bis(3-ethylbenzthiazoline-6-sulfonic acid radical scavenging activities of defatted sesame meal (IC50 ) were 891.84 and 340.09 ㎍/mL. According to the test results, the defatted sesame meal extracted using 70% ethanol had significant antioxidant activity and inhibitory ability to diabetes-related enzymes, indicating that it has good potential as a functional food or nutritional food for prevention and treatment of oxidation.

Late Holocene Paleoceanography from Core Sediments in the Admiralty Bay and Maxwell Bay, King George Island, Antarctica (남극 킹 죠지 섬 에드미럴티 만과 멕스웰 만 시추 퇴적물의 홀로세 후기 고해양환 경 연구)

  • 박병권;윤호일
    • 한국해양학회지
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    • v.30 no.4
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    • pp.302-319
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    • 1995
  • The geochemical properties, sedimentation rates, foraminiferal distributions, and oxygen and carbon isotope records of sediment from Cores S-2 and S-19 were studied to investigate late Holocene paleoceanographic and paleoclimatic changes of the admiralty and Maxwell Bay, King George Island, Antarctica. Total organic carbon contents increased from the lower part to the upper part of Cores S-2 and S-19, whereas calcium carbonate contents decreased from the lower part to the upper part of Cores S-2 and s-19,whereas calcium carbonate contents decreased from the lower part to the upper part of Cores S-2 and S-19. Twenty-seven foraminiferal species were identified, and Globocassidurina biora was mostly a bundant in sediment samples. The sedimentation rates ranged from 24 cm/kyr to 237 cm/kyr based on /SUP 14/C-age dating of G. biora. The sedimentation rates increased rapidly in the upper part of the Cores. б/SUP 18/O values ranged from 0.3% to 6.2% and б/SUP 13/C values ranged from -3.0% to 0.0% with several fluctuations of the values. The lowest part of Core S-2, at 128 cmbsf in depth, had a /SUP 14/C-age of 3,100${\pm}$60 yr B.P. and the lowest part of Core S-19, at 230 cmbsf in depth, of 7,400${\pm}$ yr B.P. The results of geochemical and sedimentological analyses of the core sediments suggested five stages of paleoceanographic and paleoclimatic changes as follows: war,-cold stage of 7,500∼6,500 yr B.P., cold stage of 6,500∼3,600 yr B.P., cold-warm stage of 3,600∼2,770 yr B.P., warm stage of 2,770∼2,380 yr B.P. and cold-warm stage of 2,380∼2,100 yr B.P.

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A study of Frequency Distributions by the Action styles (작업환경에서 오는 시력분포 및 고찰)

  • Kang, Hyang Nyeo;Kim, Sung Tae
    • Journal of Korean Ophthalmic Optics Society
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    • v.5 no.2
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    • pp.99-105
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    • 2000
  • The classifying distribution by the action style was opticion 100, photoartist 100, college student 100 for the simple myopic 35%, for the simple myopic astigmatism 2%, for the compound myopic astigmatism 30.6% for the mixed astigmatism 62.6% for the compound hyperopic astigmatism 37.4% for the ratio of emmetropia 22.4% respectively. The retractive erroreye were -0.50~-2.00Dptr for the simple myopia 14%, -2.00~-6.00Dptr 16%, -6.00Dptr 5%, C-0.25(90.180)~C-2.00DptrAxis(90, 180) 2% for the simple myopicastigmatismS-0.25C-0.25DptrAxis(90, 180)~S-1.00C-1.00DptrAxis (90, 180) 22.6%, S-1.00C-1.00 DptrAxis (90, 180)~S-2.00C-2.00DptrAxis(90, 180) 8% for the compound myopic astigmatism. S+0.25C -0.25DptrAxis(90, 180)~S+2.00C-2.00DptrAxis(90,180) 6.2% for the mixed astigmatism. S+0.25C+0.25DptrAxis(90,180)~S+1.00C+1.00DptrAxis(90,180) 3.4%, S+1.00C+1.00DptrAxis(90,180)~S+2.00C+2.00DptrAxis(90,180) 0.34% for the compound hyperopic astigmatism.

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The Effect of Hominis Placenta Herbal Acupuncture on Bell's palsy (자하거약침(紫河車藥鍼)의 Bell's palsy에 대한 치험보고(治驗報告))

  • Yun, Jeong-Hun;Yook, Tea-Han;Song, Beom-Yong
    • Journal of Pharmacopuncture
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    • v.3 no.1
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    • pp.89-99
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    • 2000
  • This report was done to observe the effect of Hominis placenta herbal acupuncture on Bell's palsy. The study group comprised 16 patients who arrived at Woo-suk university oriental hospital from January, 1999 till January, 2000 for Bell's palsy. All patients were divided into two group. One was herbal acupunture group, and the other was control group. Acupunture group was done herbal acupuncture therapy on the facial acupuncture points. Followings are achievement and a term of each group. In herbal acupuncture group, 100% motor recovery was 7 case, 75% was 1 case, and 25% motor recovery term was $7.38{\pm}5.21$ days, 50% was $11.00{\pm}6.16$ days, 75% was $15.13{\pm}9.55$ days, 100% was $23.14{\pm}7.97$ days. In control group, 100% motor recovery was 4 case, 75% was 2 case, 25% below was 2 case and 25% motor recovery term was $11.17{\pm}4.96$days, 50% was $18.17{\pm}6.82$ days, 75% was $29.50{\pm}6.95$ days, 100% was $44.00{\pm}11.49$ days. The above results indicate that Hominis placenta herbal acupuncture is a useful effect on Bell's palsy. thus, continuous herbal acupunture study will be needed for more clinical application on Bell' palsy.

Antioxidant Properties and Ubiquinone Contents in Different Parts of Several Commercial Mushrooms (시판버섯의 부위별 항산화능과 유비퀴논 함량)

  • Hong, Myung-Hee;Jin, Yoo-Jeong;Pyo, Young-Hee
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.41 no.9
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    • pp.1235-1241
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    • 2012
  • Antioxidant properties and antioxidant compound contents in different parts of 14 commercial mushrooms were evaluated. Methanolic extracts from the entire mushroom, the pileus and the stipe, separately, were screened for their DPPH and ABTS free radical scavenging activities. Total ubiquinones (Coenzyme Qs; CoQs), total phenolic, and flavonoid contents were determined, in order to assess the extract's antioxidant activity. The portion of the mushroom selected had an effect on the results, with pileus methanolic extract exhibiting the greatest antioxidant effect (p<0.05). The analyzed mushrooms contained powerful antioxidants such as phenols (144.5~536.6 mg of gallic acid equivalents, mg GAE/100 g of dried weight, dw), flavonoids (3.7~31.2 mg of quercetin equivalents, mg QE/100 g dw) and ubiquinones (65.6~485.1 ${\mu}g$/100 g dw). Content of CoQ9 and CoQ10 in the 14 commercial mushrooms varied from 23.1 to 256.2 ${\mu}g$/100 g and from 16.1 to 238.3 ${\mu}g$/100 g, respectively. Phellinus linteus showed the highest antioxidant activity among all species due to the contribution of antioxidants such as phenols (530.5 mg GAE/100 g dw) and ubiquinones (308.8 ug/100 g dw). A positive linear correlation was demonstrated between free radical scavenging activity and total phenolic ($R^2=0.79$) and ubiquinone ($R^2=0.59$) contents in the pileus of mushrooms (p<0.05). Our data indicate that commercial mushrooms have potential as dietary sources of CoQs and phenolic antioxidants.

Vitrification and Ultrarapid Freezing of Day 2 Mouse Embryos (제 2일째 생쥐 배아의 초자화동결과 초급속동결)

  • Yang, Jung-Sook;Sohn, Cherl;Bae, In-Ha
    • Clinical and Experimental Reproductive Medicine
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    • v.27 no.3
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    • pp.283-289
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    • 2000
  • Objective: The study was performed to compare the survival rate and the development of day 2 mouse embryos which had freezing procedures done. Methods: We used three different vitrification solutions (EFS, VS14, DPS) and a ultrarapid freezing solution (UFS) for cryopreservation of day 2 mouse embryo. Results: We tested toxicity by exposing embryos to vitirification solutions and a ultrarapid freezing solution. The survival rates are 100%, 97.8%, 95.6% and 100% (EFS, VS14, DPS and UFS). After cultured for 96 hours, hatching rates of each group are 93.5% (no freezing), 95.6% (EFS), 86.4% (VS14), 93.0% (DPS), and 93.0% (UFS). There is no significant differences among groups. The survival rates after thawing cryopreserved embryos are 80.2%, 91.7%, 69.5%, 0% and 91.8% (slow freezing, EFS, VS14, DPS and UFS). Also cultured for 96 hours, the hatching rates are 93.5% (no freezing), 84.1% (slow freezing), 93.9% (EFS), 48.5% (VS14) and 70.1% (UFS). Conclusion: The survival rates of vitrification in EFS solution and ultrarapid freezing are higher than slow freezing (p<0.05). The hatching rate of vitrification in EFS solution cultured for 96 hours is highest, so vitrification of day 2 mouse embryos in EFS solution considered as more effective for cryopreservation.

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