• 대한수의학회지
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• 제12권1호
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• pp.91-95
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• 1972

Investigation of leptospiral antibody in Korean cattle and pigs was carried out from February to October, 1971. Ten different living antigens, namely L. icterohaemorrhagiae, L. canicola, L. antumnalis, L. hebdomadis, L. australis A, L. pomona, L. pyrogenes, L. grippotyphosa, L. bataviae and L. javanica, were used. A total of 590 Korean cattle and 460 pig blood samples collected from Seoul Majang-dong slaughterhouse were tested by the rapid microscopic agglutination test. Throughout the studies the following results were obtained and summarized. 1. Of 590 serum samples of Korean cattle 51 were positive(8.64%). 2. Of 460 serum samples of pigs 27 were positive (5.87%). 3. Of 51 positive cattle samples, 29(4.92%) showed antibody to a serotype of L. icterohaemorrhagiae and 18(3.0%) to L. canicola, and 4 (0.68%) to L. pomona. Eight of L. icterohaemorrhagiae positive samples showed a cross reaction to L. canicola. 4. Of 27 positive pig samples, 14(3.04%) showed antibody to L. ictereohaemorrhagiae and 7(1.52%) to L. grippotyphosa. 4(0.87%) to L. canicola, 2(0.43%) to L. pomona. Two of L. canicola positive samples showed a cross reaction to L. grippotyphosa. 5. Serum samples of seven pigs, showing antibody positive to L. grippotyphosa were first observed in Korea. 6. Infection rate of bovine and porcine leptospirosis, in Korea, appeared to be lower than that of Japan, Taiwan, Thailand and the Philippines.

• 한국식생활문화학회지
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• 제14권2호
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• pp.167-175
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• 1999

This study examines the effects of the Korean horticultural foods which are, by traditional wisdom, supposed to contain anti-aging substance. All twenty three kinds of plants are chosen as a sample for the experiment in which the content of three main anti-aging nutrients, -carotene, Vitamin C, and Vitamin E are respectively measured by Nilis, Colorimetric, and AOAC method. The result shows: among the samples, top six plants that contain the most -carotene content are high quality tea(Camellia sinensis), thistle(Cirsium japonicum Var.), Angelica keiskei, mulberry leaves(Morus alba l.), field horsetail(Equisetum arvensa), and carrot(Daucus carota), in order; top four for a Vitamin C contents are low quality tea, mulberry leaves, pine leaves(Pinus densiflora), and high quality tea, in order; top four for a Vitamin E contents are persimmon leaves(Diospyros mongolicum) tea, high quality tea, low quality tea, and pine leaves, in order. Drying or boiling process increases the content of -carotene and Vitamin E in high quality tea, dropwort(Oenanthe javanica), field horsetail, and wild aster(Ligularia stenocephala Marsumum et Koldzumi), while, in case of mulberry leaves, drying process lowers them. Vitamin C content strikingly decreases in heating and drying process.

• The Korean Journal of Ecology
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• 제21권5_1호
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• pp.435-448
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• 1998

The phytosociological investigation was carried out at 32 sites of the riparian vegetation in Musim-Chon stream passed through Choengju-City, Chungcheongbuk-Province by Braun-Blanquet's method from August to October, 1997. The flora of vascular plants in this survey was 202 species. The riparian vegetation was classified into 25 communities as follows. The submerged hydrophytes are Potomogeton malaianus var. latifolius community, Potomogeton crispus community, Myriophyllum verticillatum community-Hydrilla verticillata community and Ceratophyllum demersum community. The floating-leaved hydrophyte is Trapa japonica community. The emerged hydrophytes are Phragmites communis community, Perzicaria thunbergii community, Phragmites japonica community, Oenanthe javanica community, Zizania latifolia community and Persicaria hydropiper community. The herbaceous vegetation of floodplain are Artemisia montana community, Humulus japonicus community, Miscanthus sacchariflorus community, Erigeron anmus community, Impatiens balsamina community, Bidens frondosa community, Setaria viridis community, Ambrosia artemisiifolia var. elatior community and Eragrostis ferruginea community. The woody vegetation of floodplain are Robinia pseudo-acacia community, Stephanandra incisa community and Clematis apiifolia community. The species diversity was low in the upper and down stream, while the diversity was high in the mid stream situated at the center of Choengju-City because of frequent disturbance by citizens. These results suggested that distribution of communities and species diversity was closely related to the disturbance.

• 한국염색가공학회지
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• 제24권4호
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• pp.260-269
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• 2012

The aim of this study was to develop higher-value added dyeing materials with the fermentation-dyeing -mordanting system using only the natural ingredients by integrating traditional fermentation techniques with traditional dyeing technique. Nuruk, which is used mainly to ferment traditional foods, was used as a fermenting agent to ferment 5 different types of materials. Acidic burnt alum and alkaline calcium hydroxide were used as natural mordants. The dyeability checked after fermentation showed that both cotton and silk mordanted with Gardenia jasminoides did not show notable changes, and 10 days of fermentation was found to be appropriate. Sophora japonica L. performed better on cotton materials mordanted with slaked lime, and alkaline mordants were found to be more effective than acidic ones. With Rheum coreanum, a fermentation period of more than 24 days ($5^{th}$ fermentation) worked best on cotton material, showing a 5 fold increase in the K/S value after the $5^{th}$ slaked lime fermentation than with no fermentation. Rhus javanica L. was found to increase the color fastness to gentle washing and the fastness to light was found to possess 4 means that natural dyed fabric have the highest level of durability, the grade 1 the lowest level of fastness.

• 동아시아식생활학회지
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• 제16권3호
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• pp.281-291
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• 2006

This study investigated the effect of the powder of the small water dropwort(PSWD) and brewer's yeast(BY) on the liver function and serum lipid metabolism in alcohol-consuming rats. Male Sprague-Dawley rats were fed an AIN-93 diet(control), ethanol plus control(A), ethanol plus PSWD(AS), ethanol plus BY(AB), ethanol plus PSWD and BY(ABS) diet for seven weeks. The feed intake, body weight gain and feed efficiency ratio were the lowest and in the alcohol-consuming groups. The weight of the liver, kidney, spleen and epididymis of the anatomized rats showed positive correlation with the body weight gain. Compared with group A, the content of the lipid in the serum was significantly low in the AS and AB groups. In particular, it was the most effective in the AB group. The GOT, GPT and ALP level in the serum showed a significant difference in the alcohol-consuming groups. Compared with the A group, they showed a significantly low difference in the AS and AB groups. The total cholesterol and triglyceride levels in the liver were similar in all groups. The weight of the feces was significantly different but there was no significant difference in the content of total cholesterol and triglyceride levels in the AS and AB groups. These results suggest that BY and PSWD improve the liver function, and had an effect on reducing the lipid content of the serum and feces of alcohol-consumed rats. In particular, the effect of BY which contained protein, dietary fiber and vitamin B was higher than that of PSWD.

• 한국환경복원기술학회지
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• 제5권4호
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• pp.31-38
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• 2002

The rate of sewage treatment in South Korea was 68% in the late 1999. Sewage treatment is mostly made near big cities such as Seoul and Busan, and it is little in rural areas. Wastewater from households goes to streams directly without treatment in rural areas and pollutes streams. It is necessary to improve the progress for treatment of nutrients such as N and P which cause eutrophication in streams and lakes, because sewage treatment system in South Korea focuses on treatment of basic organic matters. Therefore it will contribute to improve discharged water quality if small sewage treatment systems by aquatic plants and microbes are introduced to rural areas where are not connected to local sewage treatment facility. This experiment was conducted to find out the best way using aquatic plants and microbes to purify wastewater from households through individual sewage treatment system. Phragmites communis, Iris pseudoacorus, Acorus calamus var. angustatus, Typha orientalis and Oenanthe javanica were used for this experiment. BOD, COD, SS, T-N and T-P were analyzed following standard methods for wastewater. The result shows that wastewater was roughly purified through pebbles and sands, and highly purified through aquatic plants and microbes especially in T-N and T-P. Iris pseudoacorus is the most effective in reduction of COD and BOD level. This system will work even in winter when plants die because microbes will be still working.

• 한국미생물·생명공학회지
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• 제30권2호
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• pp.177-183
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• 2002

40여종의 한약재추출물을 제조하여 가금질병에 관련이 있는 세균에 대한 항균활성을 조사하였다. 오미자, 천련자, 소목 및 오배자 추출물은 Salmonella gallinarum에 대해 높은 항균활성을 보였으며 향유, 육두구, 초두구, 지실, 하고초, 소목 및 오배자 추출물은 Staphylococcus aureus에 대해 높은 항균활성을 보였다. 천련자, 소목 및 오배자 추출물의 S. gallinarum에 대한 최소저해농도는 1.2 ㎎/ml이며 오배자 추출물의 S. aureus에 대한 최소저해농도는 0.6 mg/ml로 가장 낮은 농도에서 항균활성을 보였다. 열에 의한 항균활성 변화에 있어서 오미자와 오배자 추출물은 S. gallinarum에 대한 항균럭이 감소하였고 초두구와 소목 추출물은 S. aureus 에 대해 항균력이 안정하였다. pH에 의한 항균활성의 변화에서 S. gallinarum에서 오미자가, S. aureus에서는 향유, 지실 및 오배자는 산성역에서는 비교적 안정하였으나 알칼리역에서 불안정하였으며 소목은 산성과 알칼리역에서 안정하였다. 항균력이 가장 우수한 오배자와 소목 추출물의 미생물 증식억제 효과를 조사하기 위해 증식배지에 0, 100, 300 및 500 ppm의 추출물을 첨가하여 균주의 증식 억제효과를 조사하였다. 배양후 24시간에 무첨가구에 비해 100 ppm이상의 오배자 추출물 첨가시 시험 균의 증식을 현저히 억제하였고, 소목 추출물에서도 무첨가구보다 300 ppm 이상의 추출물 첨가시 시험 균의 증식을 현저히 억제하였다. 이런 결과를 종합하면 가금 질병을 유발하는 S. gallinarum과 S. aureus를 예방 할 수 있는 사료첨가제 개발 가능성을 확인하였다.

• 생태와환경
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• 제35권4호통권100호
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• pp.285-294
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• 2002

본 연구는 농업용저수지의 수질개선을 위한 인공습지 시스템에서 수리학적 부하조건과 수질정화 특성간의 상관관계를 평가하고, 습지의 조성과 관리에 관한 기본적이며 주요 인자들을 규명하고자하였다. 인공습지시스템은 저수지 중층수를 유입시키기 위한 양수장과 미나리, 창포, 줄, 부들, 갈대등의 정수식물을 식재한 6개의 개별습지로 구성되어 있다. 시스템은 자유수면흐름방식으로 유입처리유량 $0.012-0.122\;m^3/s$,수리학적 체류시간 0.5-2.0 hr의 수리학적 고부하조건으로 운영하였으며, 수심은 0.1-0.2m, 유입수질은 저수지를 대상으로 하여 비교적 낮은 영양염류 농도(TN 2.224-2.462 mg/L, TP 0.145-0.164 mg/L)를 가지고 있다. 본 연구기간 중 각 개별습지의 평균 수질정화효율은 TN 12.1-14.3%로 갈대조에서 높게 나타났으며, TP는 6.3-9.5%로 식물 종에 따른 른 차이가없었다. SS는 17.4-38.5%, Cht-a는 12.6-20.2%로 미나리조에서 높게 나타났는데, 이는 유입수 농도가 다소 높은 때문으로 판단된다. 시간당 정화량은 TN $1.299-2.343\;g{\cdot}m^{-2}{\cdot}d^{-1}$ TP $0.85-1821\;g{\cdot}m^{-2}{\cdot}d^{-1}$, SS는 $17.9-111.6\;g{\cdot}m^{-2}{\cdot}d^{-1}$. Chl-3는 $0.011-0.094\;g{\cdot}m^{-2}{\cdot}d^{-1}$로 정화효율에서와 달리 TN은 줄에서 가장 높았고, TP는 창포에서 높았다. 침강성 물질인 SS와 Chl-a는 미나리에서 높게 나타났으며, 미나리는 BOD, COD, TN, TP등 다른 수질항목에서도 높은 값을 보여주고 있어 정화효율에서와 같이 유입수농도가 습지내 물질제거에 영향이 있음을 보여준다. 정화효율 및 시간당 정화량과 수리학적 조건간의 상관관계는 수심, 체류시간, 일유입량, 수리학적 부하량, 유입수 농도, 온도 등 다양한 변수에 의한 영향으로 비교적 낮게 나타났다. 정화효율과 수리학적조건간의 상관계수($R^2$)는 수리학적 체류시간과 0.016-0.731,일처리유량과는 0.015-0.868을 나타내었으며, 시간당 정화량과 수리학적 조건간의 상관계수($R^2$)는 수리학적 체류시간과는 0.173-0.763,일처리유량과는 0.209-0.770의 범위를 나타내었다. 정화효율과 수리학적 부하조건간의 상관계수($R^2$)Tt 0.5 이상을 나타내는 각 수생식물 습지별 수질항목은 체류시간과 일처리유량에 대해각각 20%,정화속도와 수리학적 조건간의 상관계수는 체류시간에 대해 53%, 일처리유량에 대해73%가 0.5이상을 보이고 있어 시간당 정화량과 수리학적 조건간의 상관관계가 정화효율과의 상관관계보다 좀더 유의성 있게 나타났다. 이것은 높은 수리학적 부하조건이 영양염류 등의 정화효율에는 크게 영향을 미치지 않음을 보여주고 있으며, 따라서 비교적 낮은 농도의 영양염류를 가지고 있고, 많은 처리수량을 요구하는 부영양화된 저수지의 수질개선을 위해서는 높은 수리학적 부하조건에서 시간당 정화량을 늘리는 관리방법이 경제적이며, 이에 초점을 맞추어 나가야 할 것으로 사료된다.

• 생약학회지
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• 제44권2호
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• pp.193-199
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• 2013

We investigated the antioxidative and antimicrobial activities of the methanol extracts from Korean folk plants (MKs) in Chungcheong Province. Among 30 MKs, 16 plants at $100{\mu}g/ml$ showed over 90% scavenging activity of 1,1-diphenyl-2-picrylhydrazy (DPPH) and 30 plants exerted the hydroxyl radical scavenging effect over 55%. Fourteen plants at the concentration $50{\mu}g/30{\mu}l$ showed strong microbial inhibition activity against Escherichia coli and Staphylococcus aureus, with clear zone greater than 11 mm in disc assays. Furthermore, the protective effect against anti-inflammatory system using RAW 264.7 macrophage cell was also studied. The treatment of LPS & INF-${\gamma}$ to RAW 264.7 cell induced nitric oxide (NO), however inhibit the formation of NO less than 50% of 5 plants. The present result indicates that the 30 species of MKs exerts protective effect of oxidative stress, antimicrobial activities and anti-inflammatory. In particular, Rhus javanica and Cornus controversa showed stronger effect on not only radical scavenging activity and inhibits growth of S. aureus but also highest protective effects from inflammation.

• 한국식품저장유통학회지
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• 제12권3호
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• pp.267-274
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• 2005

천식과 관련하여 산화스트레스 (Oxidant stress)는 그 발병요인 중의 하나로 알려져 있다. 이에 본 연구는 농산물과 한약재를 이용하여 항산화 물질을 찾고자 하였으며, 시간과 비용의 단축을 위하여 HTS인 throughput screening)을 이용을 하였다. 항산화 실험과 관련하여서 DPPH(1,1-diphenyl-2-picrylhydrazyl), FRAP(ferric ion reducing antioxidant power), HO(hydroxyl radical) 소거, linoleic acid에 대한 항산화 활성 등을 시행하였다. 이후 $H_{2}O_2$에 의한 산화스트레스를 이용한 세포사멸을 유도하여 세포생존을 확인해 보았다. 실험결과 해바라기씨(Helianthus annuus), 신선초(Angelica utilis Makino), 시금치(Rehmannia glutinosa Libo) 등이 활성이 높게 나타났다. 본 연구는 여기에서 나온 hit를 이용하여 동물모델 실험을 진행하고자 한다.