• Genes and Genomics
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• v.40 no.12
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• pp.1319-1329
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• 2018

SSRs were successfully isolated from the Perilla crop in our current study, and used to analyze Perilla accessions from East Asia. Analyses of the clear genetic diversity and relationship for Perilla crop still remain insufficient. In this study, 40 new simple sequence repeat (SSR) primer sets were developed from RNA sequences using transcriptome analysis. These new SSR markers were applied to analyze the diversity, relationships, and population structure among 35 accessions of the two cultivated types of Perilla crop and their weedy types. A total of 220 alleles were identified at all loci, with an average of 5.5 alleles per locus and a range between 2 and 10 alleles per locus. The MAF (major allele frequency) per locus varied from 0.229 to 0.943, with an average of 0.466. The average polymorphic information content (PIC) value was 0.603, ranging from 0.102 to 0.837. The genetic diversity (GD) ranged from 0.108 to 0.854, with an average of 0.654. Based on population structure analysis, all accessions were divided into three groups: Group I, Group II and the admixed group. This study demonstrated the utility of new SSR analysis for the study of genetic diversity and population structure among 35 Perilla accessions. The GD of each locus for accessions of cultivated var. frutescens, weedy var. frutescens, cultivated var. crispa, and weedy var. crispa were 0.415, 0.606, 0.308, and 0.480, respectively. Both weedy accessions exhibited higher GD and PIC values than their cultivated types in East Asia. The new SSR primers of Perilla species reported in this study may provide potential genetic markers for population genetics to enhance our understanding of the genetic diversity, genetic relationship and population structure of the cultivated and weedy types of P. frutescens in East Asia. In addition, new Perilla SSR primers developed from RNA-seq can be used in the future for cultivar identification, conservation of Perilla germplasm resources, genome mapping and tagging of important genes/QTLs for Perilla breeding programs.

• The Korean Journal of Archival Studies
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• no.19
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• pp.57-102
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• 2009

Recently, distribution of record via web and coefficient of utilization are increase. so, Archival information service using website becomes essential part of record center. The main point of archival information service by website is making record information retrieval easy. It has need of matching user's request and representation of record resources correctly to making archival information retrieval easy. Archivist and record manager have used various information representation tools from taxonomy to recent thesaurus, still, the accuracy of information retrieval has not solved. This study constructed record retrieval system based on Topic Map by modeling record resources which focusing on description metadata of the records to improve this problem. The target user of the system is general web users and its range is limited to the president related sources in the National Archives Portal Service. The procedure is as follows; 1) Design an ontology model for archival information service based on topic map which focusing on description metadata of the records. 2) Buildpractical record retrieval system with topic map that received information source list, which extracted from the National Archives Portal Service, by editor. 3) Check and assess features of record retrieval system based on topic map through user interface. Through the practice, relevance navigation to other record sources by semantic inference of description metadata is confirmed. And also, records could be built up as knowledge with result of scattered archival sources.

• Journal of the Korean Association of Geographic Information Studies
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• v.22 no.1
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• pp.93-102
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• 2019

In this study, in order to analyze the possibility of observing a typhoon track based on the Global Navigation Satellite System(GNSS), Typhoon NARI, the 11th typhoon of 2007, was analyzed in terms of the typhoon track as well as the local variation of perceptible water over time. The perceptible water was estimated using data obtained from observatories located on the typhoon track from Jeju to the southern coast of Korea for a total of 18 days from September 7(DOY 250) to September 24(DOY 267), 2007, including the period when the observatories were affected by the typhoon at full-scale, as well as one previous week and one following week. The results show that the trend of the variation of perceptible water was similar between the observatories near the typhoon track. Variation of perceptible water over time depending on the development and landing of the typhoon was distinctively observed. Several hours after the daily maximum of perceptible water was found at the JEJU Observatory, the first struck by the typhoon on the typhoon track, the maximum value was found at other observatories located on the southern coast. In the observation period, the time point at which the maximum perceptible water was recorded in each location was almost the same as the time point at which the typhoon landed at the location. To analyze the accuracy of the GNSS-based perceptible water measurement, the data were compared with radiosonde-based perceptible water data. The mean error was 0.0cm, and the root mean square error and the standard deviation were both 0.3cm, indicating that the GNSS-based perceptible water data were highly accurate and precise. The results of the this study show that the GNSS-based perceptible water data may be used as highly accurate information for the analysis of typhoon tracks over time.

• Journal of the Korea Convergence Society
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• v.10 no.1
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• pp.95-102
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• 2019

After liberation, several literary groups with the nature of the national culture movement joined in Japan in January 1948 to form the Korean-Japanese Joseon Literary Society. However, the Korean-Japanese Joseon Literary Society was unable to play an active role due to the suppression of the GHQ and gathered again as the Korean War Armistice Agreement was concluded in 1953. The institutional journal of the 'Korean-Japanese Joseon Literary Society' is published by changing the magazine name to "Munhakbo" in Japanese and "Joseon Literature", "Joseon Literature and Arts" in Korean. The national movement group of Korean residents in Japan and literature groups were reorganized in conjunction with the political situation in Japan and the Korean peninsula. The reunion of 'Korean-Japanese Joseon Literary Society' was also based on the appearance of 'Jochongnyeon (pro-Pyeongyang federation of Korean residents in Japan)' and 'conversion of line'. In this paper, we are to fill up the blank of the research on literature of Korean residents in Japan in the 1950s by identifying the reality of conflict between 'Jochongnyeon' and 'Mindan', meaning of 'Korean Writing' Movement as a subject of national movement and the issue of promoting self-esteem as a 'citizen of the Republic' that 'Korean-Japanese Joseon Literary Society' tried to convey to Korean readers in Japan.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.9
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• pp.93-102
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• 2019

This study examined the nurse's emotional exhaustion and influencing factors, and the relationship between emotional burnout and hospital nursing workload using multilevel logistic regression analysis. The study subjects were 3,083 nurses of 65 hospitals, who participated in the training conducted by the Healthcare Industry Trade Union in 2010 and responded to the questionnaire. First, 71.6% of nurses experienced emotional exhaustion, and the average score of emotional exhaustion was 33.53. Second, the 'non - nursing work experience' showed a significant effect on emotional exhaustion. Third, the 'sufficient nursing staff' variable increased the emotional exhaustion. Fourth, the nursing grades were correlated significantly with emotional exhaustion compared to those with more than four beds per nurse. Fifth, in the case of working in the internal ward, the shorter the clinical career, the higher the emotional exhaustion. In other words, higher emotional exhaustion was associated with more nursing work experience, more perceived insufficient nursing staff, more nurses per bed, the department of internal medicine, and a shorter clinical career.

• Animal Bioscience
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• v.34 no.11
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• pp.1739-1748
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• 2021

Objective: In recent years, long noncoding RNAs (lncRNAs) have been identified in many species, and some of them have been shown to play important roles in muscle development and myogenesis. However, the differences in lncRNAs between Kazakh cattle and Xinjiang brown cattle remain undefined; therefore, we aimed to confirm whether lncRNAs are differentially expressed in the longissimus dorsi between these two types of cattle and whether differentially expressed lncRNAs regulate muscle differentiation. Methods: We used RNA-seq technology to identify lncRNAs in longissimus muscles from these cattle. The expression of lncRNAs were analyzed using StringTie (1.3.1) in terms of the fragments per kilobase of transcript per million mapped reads values of the encoding genes. The differential expression of the transcripts in the two samples were analyzed using the DESeq R software package. The resulting false discovery rate was controlled by the Benjamini and Hochberg's approach. KOBAS software was utilized to measure the expression of different genes in Kyoto encyclopedia of genes and genomes pathways. We randomly selected eight lncRNA genes and validated them by quantitative reverse transcription polymerase chain reaction (RT-qPCR). Results: We found that 182 lncRNA transcripts, including 102 upregulated and 80 downregulated transcripts, were differentially expressed between Kazakh cattle and Xinjiang brown cattle. The results of RT-qPCR were consistent with the sequencing results. Enrichment analysis and functional annotation of the target genes revealed that the differentially expressed lncRNAs were associated with the mitogen-activated protein kinase, Ras, and phosphatidylinositol 3-kinase (PI3k)/Akt signaling pathways. We also constructed a lncRNA/mRNA coexpression network for the PI3k/Akt signaling pathway. Conclusion: Our study provides insights into cattle muscle-associated lncRNAs and will contribute to a more thorough understanding of the molecular mechanism underlying muscle growth and development in cattle.

• Korean Journal of Veterinary Service
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• v.44 no.2
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• pp.93-102
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• 2021

In this study, a total of 9,449 hard ticks were collected once a month from April to October 2020 from a neighborhood park in Daejeon by flagging & dragging method and CO₂ manned trap method. The collected ticks were classified according to the Yamagutsi search table using a stereoscopic microscope and molecular biological analysis of four pathogens (SFTSV, Anaplasma spp., Ehrlichia spp., Borrellia spp.). As a result of the study, Haemaphysalis longicornis were collected the most in all areas of the five boroughs at a rate of 82 to 96 percent, while adults were collected the most in May to July, nymphs were collected the most in April to June, and larvae from August to October at a rate of 78 percent to 98 percent. In pathogens, three cases of SFTSV were detected, showing a minimum infection rate (MIR) of 0.46%, while Anaplasma spp. and Ehrlichia spp. were detected one each, with 0.15% and Borrelia spp. with a minimum infection rate of 0.46%. The detected SFTSV showed 99.9% homogeneity with the KF781490 detected in Cheongwon-gun, Chungbuk Province, Anaplasma spp. showed 99.0% homogeneity with JN990105 detected in China, and Erhlichia spp. showed 98.9% genetic similarity with U96436 separated from the U.S. In this study, the distribution status and pathogen infection rate of the hard ticks in the Daejeon area are analyzed and provided as basic data for the prevention of the hard tick-borne infectious disease.

• Journal of the Korean Society of Food Culture
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• v.36 no.1
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• pp.92-102
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• 2021

There is little information on the nutrient intake according to the city size and small town in Korean elderly. This study analyzed the nutritional consumption of older people in metropolitan, middle and small cities, and rural areas according to four income levels. The recent data from the 2016~2018 Korean National Health and Nutrition Survey, Centers for Disease Control and Prevention were used. The final analysis included 4,325 individuals (Male: 1,856, Female: 2,469) over 65 years old. Multivariable regression with a complex sample design was conducted to compare the nutrient intake among the groups. In a comparison within regions, the nutrition status of the elderly in small towns was more vulnerable than metropolitan and middle & small cities. The energy intakes were similar between the groups. The carbohydrate intake of middle & small cities was significantly higher than the other regions. The intake of other nutrients in metropolitan and middle & small cities appeared to be higher than in rural areas. The number of nutrients with statistical significance between low and high-income levels were 19 in metropolitan, 11 in middle & small cities, and 5 in rural areas. Each contribution of carbohydrate, fat, and protein to the total energy intake was lower in the low-income level than the high-income level in metropolitan and middle & small cities. On the other hand, in rural areas, only the contribution of protein to energy intake was lower in the low-income level than the high-income level. Cities with higher levels of urbanization had more severe nutritional inequality in relation to the income level. There was also nutritional inequality present in rural areas but it was to a lesser extent. Moreover, the generally low level of nutrient intake was problematic in rural areas. These findings could be used as fundamental evidence for developing community nutritional policies for the elderly.

• Journal of Korean Library and Information Science Society
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• v.52 no.4
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• pp.79-102
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• 2021

This research is based on open data from the National Library Statistics System and Seoul Survey 2020 from Seoul to analyze the Seoul citizens' usage of public libraries. For such purposes, this research compared and diagnosed the condition of public libraries services at 25 districts of Seoul, focusing on objective indicators of library management and general usage records to understand the correlations between the two. Objective indicators such as budgets, human labor, the number of books, and total ground area were compared with usage records of different classes of Seoul citizens, organized by the citizens' place of living, educational background, and monthly household income. As a result of the research, the correlation between the objective indicators and public usage records of different classes was confirmed. Seoul citizens' usage of public libraries and reading tendency were identified. In addition to, a balanced development of the public libraries in Seoul was proposed to overcome regional disparities and leveling the service level by investigating usage behavior and reading tendency of the public libraries in Seoul.

• Journal of Korean Foot and Ankle Society
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• v.26 no.2
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• pp.95-102
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• 2022

Purpose: This study was based on the Korean Foot and Ankle Society (KFAS) member survey and aimed to report the current trends in the management of syndesmotic injuries over the last few decades. Materials and Methods: A web-based questionnaire containing 36 questions was sent to all KFAS members in September 2021. The questions were mainly related to the preferred techniques and clinical experiences in the treatment of patients with syndesmotic injuries. Answers with a prevalence ≥50% of respondents were considered a tendency. Results: Seventy-six (13.8%) of the 550 members responded to the survey. The results showed that the most preferred method to diagnose a syndesmotic injury was magnetic resonance imaging (MRI). Intraoperatively, the external rotation stress test and the Cotton test were most frequently used to confirm syndesmotic diastasis. The reduction was usually done by a reduction clamp. One 3.5-mm screw was used most frequently over three cortices at 2~4 cm above the ankle joint. The preferred ankle position during fixation was 0° dorsiflexion. Removal of the syndesmotic screw was routinely done by most surgeons, mainly because of the limitation of movement and risk of screw breakage. Factors that affect suture button selection included non-rigid fixation which enables adequate fixation, early weight-bearing, and an infrequent need to remove the hardware. Inadequate reduction was considered the main factor that affects poor prognosis. Conclusion: This study proposes updated information about the current trends in the management of syndesmotic injuries in Korea. Consensuses in both the diagnostic and therapeutic approach to patients with syndesmotic injury were identified in this survey study. This study may raise the awareness of the various possible approaches toward the injury and should be used to further establish a standard protocol for the management of syndesmotic injuries.