• Title/Summary/Keyword: S-102

Search Result 2,385, Processing Time 0.048 seconds

Selection of indigenous starter culture for safety and its effect on reduction of biogenic amine content in Moo som

  • Tangwatcharin, Pussadee;Nithisantawakhup, Jiraroj;Sorapukdee, Supaluk
    • Asian-Australasian Journal of Animal Sciences
    • /
    • v.32 no.10
    • /
    • pp.1580-1590
    • /
    • 2019

  • Objective: The aims of this study were to select one strain of Lactobacillus plantarum (L. plantarum) for a potential indigenous safe starter culture with low level antibiotic resistant and low biogenic amine production and evaluate its effect on biogenic amines reduction in Moo som. Methods: Three strains of indigenous L. plantarum starter culture (KL101, KL102, and KL103) were selected based on their safety including antibiotic resistance and decarboxylase activity, and fermentation property as compared with a commercial starter culture (L. plantarum TISIR543). Subsequently, the effect of the selected indigenous safe starter culture on biogenic amines formation during Moo som fermentation was studied. Results: KL102 and TISIR 543 were susceptible to penicillin G, tetracycline, chloramphenicol, erythromycin, gentamycin, streptomycin, vancomycin, ciprofloxacin and trimethoprim (MIC90 ranging from 0.25 to $4{\mu}g/mL$). All strains were negative amino acid-decarboxylase for lysis of biogenic amines in screening medium. For fermentation in Moo som broth, a relatively high maximum growth rate of KL102 and TISIR543 resulted in a generation time than in the other strains (p<0.05). These strain counts were constant during the end of fermentation. Similarly, KL102 or TISIR543 addition supported increases of lactic acid bacterial count and total acidity in Moo som fermentation. For biogenic amine reduction, tyramine, putrescine, histamine and spermine contents in Moo som decreased significantly by the addition KL102 during 1 d of fermentation (p<0.05). In final product, histamine, spermine and tryptamine contents in Moo som inoculated with KL102 were lower amount those with TISIR543 (p<0.05). Conclusion: KL102 was a suitable starter culture to reduce the biogenic amine formation in Moo som.

  • https://doi.org/10.5713/ajas.18.0596 인용 PDF KSCI

Isolation and Identification of Lactic Bacteria Containing Superior Activity of the Bile Salts Deconjugation (담즙산 분해능이 뛰어난 젖산균의 분리 및 동정)

  • 하철규;조진국;채영규;허강칠
    • Food Science of Animal Resources
    • /
    • v.24 no.2
    • /
    • pp.164-170
    • /
    • 2004

  • The purpose of this study is to isolate probiotic lactic acid bacteria (LAB) that produced bile salts hydrolase. One hundred twenty strains were initially isolated from human feces. Based on their resistance of acid, tolerances of bile salts, and inhibitory activity against Escherichia coli, five strains were selected. A strain producing highest activity of bile salts hydrolase was identified as Lactoacillus plantarum using API carbohydrate fermentation pattern and 16S rRNA sequences, and named CK102. Lactobacillus plantarum CK102 survived at a level of 1.36${\times}$10$\^$8/ CFU/$m\ell$ in pH 2 buffer for 6 h and showed exhibited excellent bile tolerance. When L plantarum CK102 was cultured with E. coli in MRS broth, no viable cells of E. coli was detected after 18 h fermentation. These results suggest that Lactobacillus plantarum CK 102 may be commercially used for the probiotic culture.

  • PDF KSCI

Evaluation of the Sterilization Effect of a Plasma Generator with a Flexible Electrode Structure on Staphylococcus aureus and Pseudomonas aeruginosa (황색포도알균과 녹농균에 대한 유연전극 구조를 갖는 플라즈마 발생기의 멸균효과 평가)

  • Park, Chul;Lee, Hyeok Jae
    • Korean Journal of Clinical Laboratory Science
    • /
    • v.52 no.4
    • /
    • pp.372-380
    • /
    • 2020

  • In this study, the sterilization ability of S. aureus and P. aeruginosa was evaluated using a plasma generator with a flexible electrode structure. Both strains were prepared at a concentration of 1.5×106 CFU/mL and inoculated and spread evenly on two medium plates. The medium were kept at a distance of 3 cm and 9 cm from the plasma generator and were plasma discharged from 30 sec to 10 minutes. The growth of colonies on the media, were subsequently compared with the control group. The mean colonies of S. aureus formed at a 3 cm distance were 9.2×102 (log value 2.96) CFU/mL for the 5 min discharge period and 8.0×10 (1.90) CFU/mL for the 10 min discharge period. When the medium was exposed for 5 min and 10 min at a 9 cm distance, the mean colonies of S. aureus formed were 2.16×103 (3.33) and 2.4×102 (2.38) CFU/mL, respectively. The medium containing P. aeruginosa kept at a 3 cm distance and exposed to 3, 5, 10-minute discharge, did not form any colonies. When kept at a 9 cm distance for 3 minutes, 6.0×102 (2.78) CFU/mL mean colonies were formed but no colonies were formed at exposure periods of 5 and 10 minutes. This enhanced sterilization effect was confirmed in experiments of S. aureus and P. aeruginosa using TiO2.

  • https://doi.org/10.15324/kjcls.2020.52.4.372 인용 PDF KSCI