• The Korean Journal of Blood Transfusion
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• v.29 no.3
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• pp.310-319
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• 2018

Background: Research on next-generation sequencing (NGS)-based HLA typing is active. To resolve the phase ambiguity and long turn-around-time of conventional high resolution HLA typing, this study developed a NGS-based high resolution HLA typing method that can handle large-scale samples within an efficient testing time. Methods: For HLA NGS, the condition of nucleic acid extraction, library construction, PCR mechanism, and HLA typing with bioinformatics were developed. To confirm the accuracy of the NGS-based HLA typing method, the results of 192 samples HLA typed by SSOP and 28 samples typed by SBT compared to NGS-based HLA-A, -B and -DR typing. Results: DNA library construction through two-step PCR, NGS sequencing with MiSeq (Illumina Inc., San Diego, USA), and the data analysis platform were established. NGS-based HLA typing results were compatible with known HLA types from 220 blood samples. Conclusion: The NSG-based HLA typing method could handle large volume samples with high-throughput. Therefore, it would be useful for HLA typing of bone marrow donation volunteers.

• Journal of Life Science
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• v.29 no.4
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• pp.447-454
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• 2019

Fat accumulation in adipocytes occurs through the process of adipogenesis in which preadipocytes differentiate into adipocytes. Obesity is a metabolic disorder caused by excessive accumulation of fat in the body, which increases the incidence of cardiovascular diseases, hypertension, type 2 diabetes, hyperlipidemia, and various cancers. Recently, inhibition of adipocyte differentiation was shown to be a potential antiobesity strategy. In this study, the inhibitory effect of dichloromethane fractions from Illicium verum Hooker fil. water extract on the differentiation of 3T3-L1 preadipocytes to adipocytes was investigated. Dichloromethane fractions from I. verum Hooker fil. significantly inhibited adipocyte differentiation when applied during the adipocyte differentiation process, as assessed by measuring fat accumulation using Oil-red O staining. In addition, dichloromethane fractions from I. verum Hooker fil. reduced important adipogenic transcription factors, such as CCAAT/enhancer binding protein ${\alpha}$ ($C/EBP{\alpha}$), $C/EBP{\beta}$, and peroxisome proliferator activated receptor ${\gamma}$ ($PPAR{\gamma}$). The expression of FAS and LPL, which are terminal differentiation markers of mature adipocytes, was also reduced in the 3T3-L1 adipocytes treated with dichloromethane fractions from I. verum Hooker fil. In addition, the treatment significantly inhibited mitotic clonal expansion, which is essential for adipocyte differentiation, by arresting the G1 phase of the cell cycle. Taken together, these results suggest that dichloromethane fractions from I. verum Hooker fil. may be a natural material with antiobesity effects.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.11
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• pp.18-23
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• 2018

FPSO consists of topside modularized plants for production of crude oil, and hullside structures that serve as support for the topside and storage of produced crude oil. The structural behavior of the FPSO topside module and its supporting hull depends on the interface structure that connects them, and the interface structure consists of a combination of individual unit support structures called Module Support Seat (MSS). Types of interface structures are various and, accordingly, the basic design of the FPSO topside module structure is greatly influenced, so various design methods should be considered from the initial design phase. Structural design of FPSO topside module requires consideration of the number of MSSs, connection type, and structural analysis options such as the range of finite element models, load conditions, and boundary conditions for verification of structural strength. In this study, the comparison combination cases for the above considerations were derived and the strength evaluation was performed, and the structural behavior characteristics of the topside module were compared and analyzed through a detailed review of the analysis results. The results of this study are considered to be a good reference for designing a more reliable topside module structure.

• Journal of the Korean Geosynthetics Society
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• v.17 no.4
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• pp.21-28
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• 2018

Despite a number of studies on consolidation behavior that have been carried out in the Nakdong River estuary in Busan, these are focused on the prediction of final settlement during ground improvement process, and there is almost no research on the long-term settlement occurring after that. For the practical study on the long-term settlement, the field monitored settlement data that measured over 10 years in the Busan new port facilities area were used, and the final settlement and predicted time-settlement line were constituted by hyperbolic function. As a result, the measured settlements depend on the thickness of clayey soils, and the pattern were similar to each other and good agreement with the predicted time-settlement line using hyperbolic method. Also, the settlement in the lower non-improved layer occupied 62-76% of total settlement. Information about above results would be quite helpful to understand the long-term settlement behaviors as well as the non-improved layer's settlement is more careful consideration needed at design phase.

• 재활복지
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• v.21 no.2
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• pp.247-259
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• 2017

We investigated the improvement of cognitive functions, activity of daily living (ADL), and quality of life (QoL) after hemorrhagic stroke and identified associated factors. For this research, twenty-five patients with a hemorrhagic stroke were enrolled. We measured cognitive function, activity of daily living (ADL), and quality of life (QoL) from 7 days to 12 months after onset of stroke. Then we analyzed the correlation between cognitive function and other risk factors. According to results, cognitive functions improved during 12 months with statistically significant differences. Other functions were similar to cognitive functions. Improvement of cognitive functions were correlated with age, the type of hemorrhagic stroke and Glasgow coma scale at 7 days after stroke. Overall, cognitive function in patients with hemorrhagic stroke recovered from acute to 12 months after onset of stroke. And, improvement of cognitive function at this phase were associated with age, the type of hemorrhagic stroke and GCS score at 7 days. These results would provide us an information to plan cognitive rehabilitation in patients with hemorrhagic stroke.

• 재활복지
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• v.20 no.1
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• pp.1-22
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• 2016

This research aimed to examine the problems in a current national emergency management system that does not consider the disabled in the face of manmade catastrophes and natural disasters, and to conduct an expert opinion survey to explore the direction of disabled-inclusive public disaster management system. As a result of the analysis, the respondents of the survey revealed a need for a designated government department for disaster management systems for the disabled and the experts preferred the Ministry of Public Safety and Security (50%) than the Ministry of Health and Welfare (37.5%). However, 12.5% of the surveyed experts perceived cooperation between the two Ministries, rather than selecting a certain ministry, as necessary to establish a disaster management system for the disabled. Additionally, the experts recognized the response period (43.8%) of the disaster management life cycle to be the most important phase. Thus, at the disaster response period, the experts suggested utilizing an emergency alarm system to effectively rescue the disabled in the face of disaster. Based on this discussion, the paper explores ways to establish a disabled-inclusive public disaster management system.

• Journal of Life Science
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• v.28 no.12
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• pp.1469-1476
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• 2018

Occurrence of the Warburg effect in solid tumors causes resistance to cancer chemotherapy, and targeting energy metabolisms such as aerobic glycolysis is a potential strategy for alternative treatment. Dichloroacetate (DCA), an inhibitor of pyruvate dehydrogenase kinase (PDK), shifts glucose metabolism from aerobic glycolysis to oxidative phosphorylation (OxPhos) in many cancers. In this study, we investigated the anticancer effect of DCA on a human anaplastic thyroid cancer (ATC) cell line, 8505C. We found that DCA selectively inhibits cell proliferation of the 8505C line but not of a normal thyroid line. In 8505C, the cell cycle was arrested at the G1/S phase with DCA treatment as a result of decreased antiapoptotic proteins such as $HIF1{\alpha}$, PDK1, and Bcl-2 and increased proapoptotic proteins such as Bax and p21. DCA treatment enhanced the production of reactive oxygen species which consequently induced cell cycle arrest and apoptosis. Interestingly, DCA treatment not only reduced lactate production but also increased the expression of sodium-iodine symporter, indicating that it restores the OxPhos of glucose metabolism and the iodine metabolism of the ATC. Taken together, our findings suggest that PDK inhibitors such as DCA could be useful anticancer drugs for the treatment of ATC and may also be helpful in combination with chemotherapy and radiotherapy.

• Journal of the Institute of Convergence Signal Processing
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• v.19 no.4
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• pp.153-161
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• 2018

This paper describes the performance improvement of Software (SW) digital filter using GPU (Graphical Processing Unit). The previous developed SW digital filter has a problem that it operates on a CPU (Central Processing Unit) basis and has a slow speed. The GPU was introduced to filter the data of the EAVN (East Asian VLBI Network) observation to improve the operation speed and to process data with other stations through filtering, respectively. In order to enhance the computational speed of the SW digital filter, NVIDIA Titan V GPU board with built-in Tensor Core is used. The processing speed of about 0.78 (1Gbps, 16MHz BW, 16-IF) and 1.1 (2Gbps, 32MHz BW, 16-IF) times for the observing time was achieved by filtering the 95 second observation data of 2 Gbps (512 MHz BW, 1-IF), respectively. In addition, 2Gbps data is digitally filtered for the 1 and 2Gbps simultaneously observed with KVN (Korean VLBI Network), and compared with the 1Gbps, we obtained similar values such as cross power spectrum, phase, and SNR (Signal to Noise Ratio). As a result, the effectiveness of developed SW digital filter using GPU in this research was confirmed for utilizing the data processing and analysis. In the future, it is expected that the observation data will be able to be filtered in real time when the distributed processing optimization of source code for using multiple GPU boards.

• The Korean journal of internal medicine
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• v.33 no.6
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• pp.1224-1233
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• 2018

Background/Aims: The objective of this study was to determine the efficacy and safety of add-on therapy with certolizumab pegol (CZP) in active rheumatoid arthritis (RA) patients of a single ethnicity. Methods: In this 24-week, phase 3, randomized, double-blind, placebo-controlled trial, eligible patients (n = 127) were randomized 2:1 to subcutaneous CZP + methotrexate (MTX; 400 mg at week 0, 2, and 4 followed by 200 mg every 2 weeks) or placebo + MTX. Results: At week 24, the American College of Rheumatology criteria for 20% (ACR20) response rate was significantly greater with CZP + MTX than with placebo (66.7% vs. 27.5%, p < 0.001). Differences in ACR20 response rates for CZP vs. placebo were significant from week 1 (p < 0.05) and remained significant through week 24. The CZP group reported significant improvement in physical function and disability compared to the placebo group (p < 0.001) at week 24, as assessed by Korean Health Assessment Questionnaire-Disability Index (KHAQ-DI). Post hoc analysis indicated that the proportion of patients who had ACR70 responses, Disease Activity Score 28 (DAS28) low disease activity, and DAS28 remission at week 24 was greater in CZP + MTX-treated patients who achieved a decrease in DAS28 ${\geq}1.2$ (43.8%) at week 4 than in nonresponders. Among 18 (22.2%) and 14 patients (35.0%) in CZP and placebo groups who had latent tuberculosis (TB), none developed active TB. Most adverse events were mild or moderate. Conclusions: CZP treatment combined with MTX in active RA patients with moderate to severe disease activity and an inadequate response to MTX resulted in rapid onset of efficacy, which is associated with better clinical outcome at week 24 and has an acceptable safety profile, especially in an intermediate TB-burden population.

• Korean Journal of Heritage: History & Science
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• v.49 no.4
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• pp.64-79
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• 2016

The purpose of the present study is to clarify the process of change to the pond at the Jongmyo Shrine(宗廟), from the Joseon Dynasty period to the modern and contemporary era. The consequent research results can be summarized as follows. The first record of the Jongmyo pond is confirmed in the "Annals of King Sejong(世宗實錄)". The pond, which was being constructed at the time, refers to the current upper pond(上池). A pair of islets(雙島) were constructed during the Sukjong(肅宗) period, with several trees planted on the middle islet(中池). The middle pond is thick with lotuses. In particular, the middle island on the middle pond subsequently changed into a single island(單島), which is likely to be related to the pond expansion during the reign of Youngjo(英祖). When the lower pond(下池), involving dualistic arrangement, was constructed in the modern and contemporary era, the pond of the Jongmyo Shrine underwent a drastic transformation phase. The lower pond was constructed before 1947 at the latest, and when the right side of the lower pond was filled in the mid 1980s, the dualistically arranged lower pond became a unified type of lower pond. On the other hand, the bank protections of the upper and middle ponds were constructed with earth, but was subsequently modified into its current form using stone.