• 제목/요약/키워드: S phase

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생쥐 LP1-1 세포에서 S phase 동안 nuclear matrix에 고정되어 있는 복제 끝점 (Termination Sites of fleplication Are Anchored to the Nuclear Matrix during S Phase in Mouse LPI-1 Cells)

  • 이형호;이갑열
    • 한국동물학회지
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    • 제37권3호
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    • pp.318-323
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    • 1994
  • The association of replication origins/termini with nuclear matrix during S phase was investigated by DNase digestion of halo structures in synchronized mouse LPI-1 cells. The binding of parental DNA to nuclear matrix was constant throughout S phase. When nuclear matrix was isolated from the cells pulse-labeled with 3H-thvmidine at various stases of S phase, total 3H-labels associated with nuclear matrix were specifically higher at So, Sa and Ss stages than other stases of S phase, suggesting that the newly synthesized DNAs at those stages are not excluded out of nuclear matrix. Similar patterns were obsenred from the pulse-chase experiments, in which cells were pulse-labeled at each stage of S phase and further incubated for 1 hr. These results suggest that the replication origins and termini are fixed at the nuclear matrix, and that the nuclear matrix binding fractions of DNA at 3C-pause may contain a large population of replication origins and termination sites.

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전원동기를 위한 위상검출방법 (A Phase Detection Method For Line Lock)

  • 김영춘;이사영
    • 전력전자학회:학술대회논문집
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    • 전력전자학회 2007년도 하계학술대회 논문집
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    • pp.428-430
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    • 2007
  • Converter that is dc source equipment source's phase by reference control function that detect source's phase because should be done compulsorily use. Source's phase detect method there be method that use source's ac voltage directly by signal, and use methods that voltage detects status by PLL method and so on via point that '0' becomes usually. All above methods to detect phase are using, wrong action of phase detector converter's ailment or converter of burn can. Ths paper compares and examined usable phase detection method in source's frequency fluctuation presuming source's frequency using observer.

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ZnS 미립자 분산 붕규산엽계 유리에서의 분상 및 미립자 석출 특성 (Phase Separation and Precipitation Characteristics in ZnS doped Borosilicate Glasses)

  • 이승환;류봉기
    • 한국세라믹학회지
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    • 제35권12호
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    • pp.1337-1342
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    • 1998
  • To investigate an effect of phase separation on precipitation characteristics of ZnS microcrystals in ma-trix glass ZnS doped borosilicate glasses for nonlinear optical applications were prepared by melting and pre-cipitation process. ZnS dopant contributed to phase separation promotion which increased the phase separa-tion of the matrix glass within immiscibility region. It was also found that ZnS as phase separation promoter showed a similar contribution for some selected glass compositions in miscibility region. The precipitation of ZnS microcrystals occurred in thephase separable glass compoitions. The radius of ZnS microcrystals in-creased with increasing the heat treatment temperature and Na2O contents of matrix glass composition. The ZnS particle sizes estimated by effective mass approximation ranged from about 30 to 80${\AA}$ It was suf-ficiently small to show quantum confinement effect.

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핸드스프링 몸접어 앞공중돌기동작의 운동학적 분석 (The Kinematic Analysis of Handspring Salto Forward Piked)

  • 권오석
    • 한국운동역학회지
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    • 제17권1호
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    • pp.145-153
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    • 2007
  • The purpose of this study is to compare and analyze the phase-by-phase elapsed time, the COG, the body joint angle changes and the angular velocities of each phase of Handspring Salto Forward Piked performed by 4 college gymnasts through 3D movement analysis program. 1. The average elapsed time for each phase was .13sec for Phase 1, .18sec for Phase 2, .4sec for Phase 3, and .3sec for Phase 5. The elapsed time for Phase 1 to Phase 3 handspring was .35sec on average and the elapsed time for Phase 4 to Phase 5 handspring salto forward piked was .7sec on average. And so it showed that the whole elapsed time was 1.44sec. 2. The average horizontal changes of COG were 93.2 cm at E1, 138. 5 cm at E2, 215.7 cm at E3, 369.2 cm at E4, 450.7 cm at E5, and 553.1 cm at E6. The average vertical changes of COG were 83.1 cm at E1, 71.3 cm at E2, 78.9 cm at E3, 93.7 cm at E4, 150.8 cm at E5, and 97.2 cm at E6. 3. The average shoulder joint angles at each phase were 131.6 deg at E1, 153.5 deg at E2, 135.4 deg at E3, 113.4 deg at E4, 39.6 deg at E5, and 67.5 deg at E6. And the average hip joint angles at each phase were 82.2 deg at E1, 60 deg at E2, 101.9 deg at E3, 161.2 deg at E4, 97.7 deg at E5, and 167 deg at E6. 4. The average shoulder joint angular velocities at each phase were 130.9deg/s E1, 73.1 deg/s at E2, -133.9 deg/s at E3, -194.4 deg/s at E4, 29.4 deg/s at E5, and -50.1 deg/s at E6. And the average hip joint angular velocities at each phase were -154.7 deg/s E1, -96.5 deg/s at E2, 495.9 deg/s at E3, 281.5 deg/s at E4, 90.3 deg/s at E5, and 181.7 deg/s at E6. The results shows that, as for the performance of handspring salto forward piked, it is important to move in short time and horizontally from the hop step to the point to place the hands on the floor and jump, and to stretch the hip joints as much as possible after the displacement of the hands and to keep the hip joints stretched and high in the vertical position at the takeoff. And it is also important to bend the shoulder joints and the hip joints fast and spin as much as possible after the takeoff, and to decrease the speed of spinning by bending he shoulder joints and the hip joints quickly after the highest point of COG and make a stable landing.

수핵란 세포질의 세포주기 조절에 의한 소 체외수정란의 핵이식 (Nuclear Transplantation of Bovine IVF Embryos by Cell Cycle Control of Recipient Cytoplasm)

  • 정희태;임석기;박춘근;양부근;김정익
    • 한국가축번식학회지
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    • 제20권3호
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    • pp.307-313
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    • 1996
  • This study was conducted to investigate the effect of S-phase synthronized nuclear transfer on the development of nuclear transplant bovine embryos. A blastomere derived from the 16~32 cell stage bovine embryos was transferred into an enucleated metaphase II(MII) oocytes or activated S-phase eggs. From the MII-phase and S-phase nuclear transfer, 6.3%(4/63) and 13.8%(9/65) of nuclear transplant embryos developed to the blastocyst stage, respectively. In the S-phase nuclear transfer, maximal proportion of embryos developed to the blastocyst stage(16.6%) was obtained after the recipient cell was activated 8 h prior to receving a donor nucleus. MII-phase nuclear transplant embryos showed the PCC state of their nuclear at 1.5~2 h after fusion, whereas, S-phase nuclear transplant embryos did not undergo PCC. The result of this study suggests that if blastomeres of unknown cell-cycle-stage are used, S-phase nuclear transplantation through the activation of enucleated oocytes prior to fusion enhances development of nuclear transplant embryos. This result also suggests that the interval time from oocyte activation to cell fusion may affect the development of nuclear transplant embryos.

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Fringe Projection Profilometry를 개선한 효율적인 3D 측정 기법 (An Efficient 3D Measurement Method that Improves the Fringe Projection Profilometry)

  • 김호중;조태훈
    • 한국정보통신학회논문지
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    • 제20권10호
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    • pp.1973-1979
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    • 2016
  • 기술이 발전하면서 카메라를 통해 3D 측정을 하는 방법은 계속 발전되어왔고 최근에는 여러 주기의 fringe pattern을 이용한 측정 방법을 쓰고 있다. 본 논문에서는 여러 주기의 fringe pattern을 이용한 3D 측정 방법에 대한 기존 방법의 문제점을 제시하고, 이에 대한 해결 방안으로 기준면의 절대위상과 물체의 위상차를 이용한 물체의 절대위상을 구하는 방법을 제안한다. 이를 이용하면 새로운 물체에 대해서 매번 여러 주기의 fringe pattern을 조사하지 않고 물체의 절대위상을 얻을 수 있다. 따라서 제안하는 방법을 이용하면, 측정단계에서 취득하는 영상의 개수가 적기 때문에 보다 빠른 속도로 3D 측정을 할 수 있다. 실험을 통하여 제안하는 방법의 유용성을 보였다.

C-S-H 상의 염소이온 흡착 메커니즘 규명을 위한 반응 작용 실험 (Interaction Experiment on Chloride Ion Adsorption Behavior of C-S-H Phases)

  • 윤인석
    • 콘크리트학회논문집
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    • 제29권1호
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    • pp.65-75
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    • 2017
  • C-S-H 상은 시멘트 페이스트의 50~60%를 차지하는 중요한 수화생성물로서, 시멘트 페이스트의 공학적 특성을 결정짓는 가장 중요한 역할을 한다. 이것은 C-S-H 상이 본질적으로 안정되거나 강한 재료라서가 아니라 시멘트입자와 같이 결합하여 연속적인 레이어 층을 형성하기 때문이다. 결합상으로서 C-S-H 상은 나노 단위의 구조로부터 기인하는데, 내구성 측면에서는 염소이온의 흡착을 유발하는 것으로 알려져 있지만 그 메커니즘은 여전히 불분명하다. 그래서 본 연구에서는 C-S-H상이 염소이온 흡착에 미치는 거동을 살펴보고자 하였다. 본 연구의 목적은 다양한 Ca/Si 비율을 갖는 C-S-H 상이 염소이온을 흡착하는 시간의 존적 거동을 고찰하여 염소이온 고정화의 메커니즘을 구명하는 것이다. C-S-H 상은 순간적 물리흡착, 물리 화학적 흡착, 그리고 화학적 흡착의 3단계로 구분되어 순차적인 흡착거동을 보였는데, 순간적으로 흡착되는 표면착물량은 C-S-H 표면 대전체와 염소 이온간의 전기 상호작용에 의한 물리적 흡착에 의하여 발생한다. 높은 Ca/Si 비율에서 C-S-H 표면전하는 커지기 때문에 물리적 흡착은 커지지만 화학적 흡착은 오히려 작아지는 것으로 나타났다. 이는 C-S-H 표면에 물리적 흡착된 염소이온에 의하여 염소이온이 침투하지 못하고 화학적 흡착력까지 저하되기 때문으로 생각된다. 따라서 최대 염소이온 흡착력은 Ca/Si 비율 1.5에서 형성되었다.

Strategies to Induce Metastable T' Phase of Monolayer MoS2

  • 강성우
    • EDISON SW 활용 경진대회 논문집
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    • 제6회(2017년)
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    • pp.417-423
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    • 2017
  • Monolayer $MoS_2$의 metastable phase인 T' phase가 stable한 H phase보다 안정해지는 조건을 모색하기 위해 substitutional doping을 했을 때와 strain을 걸어주었을 때의 에너지 차이를 DFT 방법으로 계산하였다. Doping을 했을 때와 strain이 있을 때 T' phase와 H phase의 에너지 차이가 감소함을 확인하였으나 H phase보다 T' phase가 안정해지는 조건을 찾지는 못하였다. 하지만 이 방법을 기존의 alkali adsorption 방법과 병행하여 기존 방법의 단점을 보완할 수 있을 것을 기대해 볼 수 있다. 또한 전자구조 분석 중 얻은 dopant의 주기와 족에 따른 경향성은 다른 TMD 물질의 phase engineering을 design할 때 universal한 design rule로서 응용할 수 있음을 기대해 볼 수 있다.

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ZnS multi-phase에 따른 발광특성 연구 (Study on the Luminescence Properties according to ZnS multi-phase)

  • 김광복;김용일;천희곤;조동율;구경완
    • 한국전기전자재료학회논문지
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    • 제14권1호
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    • pp.48-53
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    • 2001
  • The crystal structure of ZnS fabricated by gas-liquid phase reaction was refined by the Rietveld program using X-ray diffraction data. The R-weighted pattern (R$\sub$wp/) of ZnS powder was 10.85%. The fraction of HCP phase was closely related with extra amount of H$_2$S gas. The lattice parameters and crystalline size were changed by the relative ratio of multi-phase. The luminescence property of ZnS:Cu, Al green phosphors prepared by conventional methods was good in the range of 91∼94% and 150∼190${\AA}$, respectively. According to the maximum entropy electron density(MEED) methods, any defects in (001) plane of cubic phase were not found. We suggest that both the Rietveld and maximum entropy density methods may be useful tools for studying luminescence mechanism of other phosphors materials.

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A Study on Client's Decision-Making in Construction Phase of Building Projects in Japan

  • Tamura, Atsushi;Kaneta, Takashi;Furusaka, Shuzo
    • 국제학술발표논문집
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    • The 7th International Conference on Construction Engineering and Project Management Summit Forum on Sustainable Construction and Management
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    • pp.183-191
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    • 2017
  • In building project, client needs to decide many things, and clients cooperate with architects, supervisors, general contractors and other consultants. Especially in construction phase, clients often make a decision. However, client's decision-making in construction phase often makes some kinds of risk. This paper aims to clarify the reality of client's decision-making in construction phase. First, client's decision-making is defined by laws and standards. Second, the questionnaire revealed the current status of client's decision-making in 4 works. Third, Fishbone diagram shows the factors of client's decision-making in four works. Finally, it was modeled how client's decision-making in construction phase affects the post-process of 4 works.

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