• Journal of the Korea Computer Industry Society
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• v.2 no.12
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• pp.1541-1548
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• 2001

In this paper, We propose a new Kerberos certification mechanism that improve certification service based on PKINIT that announce in IETF CAT Working Group. Certification between area connected by chain through PKINIT that use X.509 and DS/DNS mutually for service. In order to provide regional services used private key and public key, X.509 of PKINIT is employed on session part and Kerberos's private key on actual authentication part. New mechanism be reduced communication overload doing to simplify certification formality between Client and remote KDC by KDC's certificate use to get ticket in remote sacred ground and remote KDC's reaffirmation process omitted.

• Animal Bioscience
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• v.36 no.10
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• pp.1488-1498
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• 2023

Objective: α_S1-Casein is more closely associated with milk allergic reaction than other milk protein components. microRNA (miRNA) is a class of small non-coding RNAs that modulate multiple biological progresses by the target gene. However, the post-transcriptional regulation of α_S1-casein expression by miRNA in ruminants remains unclear. This study aims to explore the regulatory roles of miR-380-3p on α_S1-casein synthesis in goat mammary epithelial cells (GMEC). Methods: α_S1-Casein gene and miR-380-3p expression was measured in dairy goat mammary gland by quantitative real-time polymerase chain reaction (qRT-PCR). miR-380-3p overexpression and knockdown were performed by miR-380-3p mimic or inhibitor in GMEC. The effect of miR-380-3p on α_S1-casein synthesis was detected by qRT-PCR, western blot, luciferase and chromatin immunoprecipitation assays in GMEC. Results: Compared with middle-lactation period, α_S1-casein gene expression is increased, while miR-380-3p expression is decreased during peak-lactation of dairy goats. miR-380-3p reduces α_S1-casein abundance by targeting the 3'-untranslated region (3'UTR) of α_S1-casein mRNA in GMEC. miR-380-3p enhances β-casein expression and signal transducer and activator of transcription 5a (STAT5a) activity. Moreover, miR-380-3p promotes β-casein abundance through target gene α_S1-casein, and activates β-casein transcription by enhancing the binding of STAT5 to β-casein gene promoter region. Conclusion: miR-380-3p decreases α_S1-casein expression and increases β-casein expression by targeting α_S1-casein in GMEC, which supplies a novel strategy for reducing milk allergic potential and building up milk quality in ruminants.

• Journal of Microbiology and Biotechnology
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• v.26 no.10
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• pp.1774-1780
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• 2016

Vegetative insecticidal proteins (Vips) are insecticidal proteins synthesized by Bacillus thuringiensis during the vegetative stage of growth. In this study, Vip3Aa protein, obtained by in vitro expression of the vip3Aa gene from B. thuringiensis WB5, displayed high insecticidal activity against Spodoptera litura aside from Spodoptera exigua and Helicoverpa armigera. Bioassay results showed that the toxicity of Vip3Aa protein against S. litura larvae statistically decreased along with the increase of the age of the larvae, with LC₅₀ = 2.609 ng/cm² for neonatal larvae, LC₅₀ = 28.778 ng/cm² for first instar larvae, LC₅₀ = 70.460 ng/cm² for second instar larvae, and LC₅₀ = 200.627 ng/cm² for third instar larvae. The accumulative mortality of 100% larvae appeared at 72 h for all instars of S. litura larvae, when feeding respectively with 83.22, 213.04, 341.40, and 613.20 ng/cm² of Vip3Aa toxin to the neonatal and first to third instar larvae. The histopathological effects of Vip3Aa toxin on the midgut epithelial cells of S. litura larvae was also investigated. The TEM observations showed wide damage of the epithelial cell in the midgut of S. litura larvae fed with Vip3Aa toxin.

• Journal of the Korea Fashion and Costume Design Association
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• v.21 no.3
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• pp.109-119
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• 2019

The purpose of this study is to examine fashion trends and the direction of the four fashion collections by analyzing the design key-words of the four major international fashion collections in 2018. The data of this study was collected by extracting the key-words from Marie Claire Korea in 2018, with the total of the collected data numbering 2,144. The data was analyzed by text mining using the R program and word-cloud, and a co-occurrence network analysis was conducted. The results of this study are as follows: First, the key-words of fashion collection designs in 2018 were fringe and ruffle detail, silk and denim fabric, vivid color, stripe and check pattern, pants suit item, and oversized silhouette, focusing on romanticism and sport. Second, seasonal characteristics of the fashion collections were pastel colors in S/S, primary and vivid colors in F/W. Details were embroidery and cutouts in S/S, patchwork and fringe in F/W. Third, the design trends of the four major fashion collections were presented in the Paris collection: stripes, check patterns, embroidery, lace, tailoring, draping, romanticism, and glamor. In the Milan collection, checks, prints, denim, and minidresses reflected sport and romanticism. The London collection included fringe, ruffles, floral patterns, flower patterns, and romanticism. The New York collections included vivid colors, neon colors, pastel colors, oversize silhouettes, bodysuits, and long dresses.

• Culinary science and hospitality research
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• v.23 no.5
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• pp.58-66
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• 2017

The purpose of this study was to assess priority of educational needs for the development of curriculum based on key competencies of NCS for departments related to culinary arts in a 2-year college. In order to achieve this objective, the survey has been distributed to cooks working in hotel restaurants or restaurant companies by social network service and a total of 360 responses were analyzed excluding 18 responses. The collected surveys were analysed by using paired t-test, Borich's needs analysis and the locus for focus analysis. The results of this study were as follows. First, the difference between the level of importance and present level about key competencies recognized by cooks working in restaurant company was statistically significant in 9 fields of key competencies excluding mathematical competence. The difference recognized by cooks working in hotel was statistically significant in all 10 fields of key competencies. Second, the results of Borich's needs assessment showed that the education needs of problem solving competence were the highest, followed by interpersonal, resource management, communication, information competence. Finally, considering both Borich's needs assessment and the locus for focus analysis, cooks working in hotel restaurants recognized that educational needs of communication, interpersonal, problem-solving, information competence were the top priority, and cooks working in restaurant companies recognized that educational needs of communication, interpersonal, skill, resource management competence were the top priority. Therefore, it is necessary to develop curriculum for culinary arts specialists based on key competence of top priority.

• BMB Reports
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• v.38 no.5
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• pp.624-631
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• 2005

Tuberculosis, caused by Mycobacterium tuberculosis, continues to be one of the main diseases to mankind. It is urgent to discover novel drug targets for appropriate antimicrobial agents against this human pathogen. The shikimate pathway is onsidered as an attractive target for the discovery of novel antibiotics for its essentiality in bacteria and absence in mammalian cells. The Mycobacterium tuberculosis aroE-encoded shikimate dehydrogenase was cloned, expressed and purified. Sequence alignment analysis shows that shikimate dehydrogenase of Mycobacterium tuberculosis exhibit the pattern of G-X-(N/S)-V-(T/S)-X-PX-K, which is highly conserved within the shikimate dehydrogenase family. The recombinant shikimate dehydrogenase spectrum determined by CD spectroscopy showed that the percentages for $\alpha$-helix, $\beta$-sheet, $\beta$-turn, and random coil were 29.2%, 9.3%, 32.7%, and 28.8%, respectively. The enzymatic characterization demonstrates that it appears to be fully active at pH from 9.0 to 12, and temperature $63^{\circ}C$. The apparent Michaelis constant for shikimic acid and $NADP^+$ were calculated to be about $29.5\;{\mu}M$ and $63\;{\mu}M$. The recombinant shikimate dehydrogenase catalyzes the substrate in the presence of $NADP^+$ with an enzyme turnover number of $399\;s^{-1}$. Zymological studies suggest that the cloned shikimate dehydrogenase from M. tuberculosis has a pretty activity, and the work should help in the discovery of enzyme inhibitors and further of possible antimicrobial agents against Mycobacterium tuberculosis.

• Current Applied Physics
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• v.18 no.12
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• pp.1571-1576
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• 2018

Recent study shows that the main reason for limiting CZTS device performance lies in the low open circuit voltage, and crucial factor that could affect the $V_{oc}$ is secondary phases like ZnS existing in absorber layer and its interfaces. In this work, the $Cu_2ZnSnS_4$ thin film solar cells were prepared by sputtering CuSn and CuZn alloy targets. Through tuning the Zn/Sn ratios of the CZTS thin films, the crystal structure, morphology, chemical composition and phase purity of CZTS thin films were characterized by X-Ray Diffraction (XRD), scanning electron microscopy (SEM) equipped with an energy dispersive spectrometer (EDS) and Raman spectroscopy. The statistics data show that the CZTS solar cell with a ratio of Zn/Sn = 1.2 have the best power convention efficiency of 5.07%. After HCl etching process, the CZTS thin film solar cell with the highest efficiency 5.41% was obtained, which demonstrated that CZTS film solar cells with high efficiency could be developed by sputtering CuSn and CuZn alloy targets.

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
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• v.3 no.2
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• pp.64-69
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• 2010

This paper designs the ABC(Advanced Block Cipher) algorithm which is a 64byte block encryption algorithm, improves the performance of encryption process time, and makes an key exchange using EC-DH. The ABC algorithm reduces basic memory occupation rates using the original data position exchange method which is a data swap key without S-Box, IP-Box and etc. Also, it prepares the exposure of symmetric key using the unfixed encryption(decryption) key excepting the fixed encryption(decryption) key. Therefore, the proposed ABC algorithm in this paper is a proper encryption algorithm in lower memory environment and mobile banking.

• BMB Reports
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• v.39 no.4
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• pp.406-411
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• 2006

Signaling lymphocyte activation molecule (SLAM; also known as CD150) is a newly identified cellular receptor for measles virus (MV). The interaction between MV Haemagglutin (MVH) and SLAM is an initial step for MV entry. We have identified several novel SLAM binding sites at residues S429, T436 and H437 of MVH protein and MVH mutants in these residues dramatically decrease the ability to interaction with the cell surface SLAM and fail to co-precipitation with SLAM in vivo as well as malfunction in syncytium formation. At the same time, K58, S59 and H61 of SLAM was also identified to be critical for MVH and SLAM binding. Further, these residues may be useful targets for the development of measles therapy.

• The KIPS Transactions:PartC
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• v.11C no.5
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• pp.585-594
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• 2004

When several service providers want to work together with only one master key, they need to properly distribute the key to participants who come in for the co-work business and then securely manage the distributed keys. This paper describes the system that can efficiently and securely manage the master key on the basis of the secret sharing scheme that can reconstruct original secret information as the necessity of reconstructing original secret arises. The proposed system can distribute secret information to several groups and also redistribute the secret to subgroup in proportion to the participant's security level using smart card-based (t, t)-(k, n)-threshold secret scheme for securely keeping secret information and authentication of participant's identification.