• BMB Reports
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• v.35 no.3
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• pp.313-319
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• 2002

N-Acetyl-$\beta$-D-hexosaminidase ($\beta$-HexNAc'ase) (EC 3.2.1.52) was purified from rice seeds (Oryza sative L. var. Dongjin) using ammonium sulfate (80%) precipitation, Sephadex G-150, CM-Sephadex, and DEAE-Sephadex chromatography, sequentially. The activities were separated into 7 fractions($F_1-F_7$) by CM-Sephadex chromatography. Among them, F6 was further purified to homogeneity with a 13.0% yield and 123.3 purification-fold. The molecular mass was estimated to be about 52 kDa on SDS-PAGE and 37.4 kDa on Sephacryl S-300 gel filtration. The enzyme catalyzed the hydrolysis of both p-nitrophenyl-N-acetyl-$\beta$-D-hexosaminide (pNP-GlcNAc) and p-nitrophenyl-N-acetyl-$\beta$-D-hexosaminide (pNP-GalNAc) as substrates, which are typical properties of $\beta$-HexNAc'ase. The ratio of the pNP-GlcNAc'ase activity to the pNP-GalNAc'ase activity was 4.0. However, it could not hydrolyze chitin, chitosan, pNP-$\beta$-glucopyranoside, or pNP-$\beta$-glucopyranoside. The enzyme showed $K_m$, $V_{max}$ and $K_{cat}$ for pNP-GlcNAc of 1.65 mM, $79.49\;mM\;min^{-1}$, and $4.79{\times}10^6\;min^{-1}$, respectively. The comparison of kinetic values for pNP-GlcNAc and pNP-GalNAc revealed that the two enzyme activities are associated with a single binding site. The purified enzyme exhibited optimum pH and temperature for pNP-GlcNAc of 5.0 and $50^{\circ}C$, respectively. The enzyme activity for pNP-GlcNAc was stable at pH 5.0-5.5 and $20-40^{\circ}C$. The enzyme activity was completely inhibited at a concentration of 0.1 mM $HgCl_$ and $AgNO_3$, suggesting that the intact thiol group is essential for activity. Chloramine T completely inhibited the activity, indicating the possible involvement of methionines in the mechanism of the enzyme.

• Proceedings of the Korean Vacuum Society Conference
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• 2014.02a
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• pp.181.2-181.2
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• 2014

A single-layer graphene has been uniformly grown on a Cu surface at elevated temperatures by thermally processing a poly (methyl methacrylate) (PMMA) film in a rapid thermal annealing (RTA) system under vacuum. The detailed chemistry of the transition from solid-state carbon to graphene on the catalytic Cu surface was investigated by performing in-situ residual gas analysis while PMMA/Cu-foil samples being heated, in conjunction with interrupted growth studies to reconstruct ex-situ the heating process. We found that the gas species of mass/charge (m/e) ratio of 15 ($CH_3{^+}$) was mainly originated from the thermal decomposition of PMMA, indicating that the formation of graphene occurs with hydrocarbon molecules vaporized from PMMA, such as methane and/or methyl radicals, as precursors rather than by the direct graphitization of solid-state carbon. We also found that the temperature for dominantly vaporizing hydrocarbon molecules from PMMA and the length of time, the gaseous hydrocarbon atmosphere is maintained, are dependent on both the heating temperature profile and the amount of a solid carbon feedstock. From those results, we strongly suggest that the heating rate and the amount of solid carbon are the dominant factors to determine the crystalline quality of the resulting graphene film. Under optimal growth conditions, the PMMA-derived graphene was found to have a carrier (hole) mobility as high as ${\sim}2,700cm^2V^{-1}s^{-1}$ at room temperature, which is superior to common graphene converted from solid carbon.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.14 no.11
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• pp.5737-5744
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• 2013

This study was performed to identify the association between the menarche experience and mental health in middle school girls aged 14. The subjects were selected using data from the 8th Korean Youth Risk Behavior Web-based Survey (KYRBWS-V), which included 5,991 girls in 1st grade of middle school. Data were analyzed using the PASW 18.0 program for frequency analysis, t-test, chi square and logistic regression analysis. As to results, we found 4,473 girls who had experienced menarche (mean age, 12.8 0.9 years) and 1,518 girls hadn't experienced menarche. In the logistic regression analysis between the menarche experience and mental health behaviors, the odds ratio (OR) [95% confidence interval (CI)] for perceived stress (OR=1.39, 1.20-1.62, p<0.001), perceived depression (OR=1.25, 1.10-1.42, p<0.05), suicide thinking (6OR=1.60, 1.38-1.87, <0.001), suicide planning (OR=1.66, 1.33-2.81, p<0.001) and suicide trial (OR=1.42, 1.09-1.86, p<0.05) were higher in the menarche experience group than in the menarche non-experience group. Our results indicate, a viable association between the menarche experience and mental health in the 1st grade of middle school girls, and suggests that the menarche experience is an important factor influencing adolescent girls' mental health.

• Applied Chemistry for Engineering
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• v.23 no.5
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• pp.462-466
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• 2012

$\beta$-cyclodextrin ($\beta$-CD) polymers were prepared in a strong alkali condition solution (NaOH solution 30% (w/v)) using epichlorohydrin (EPI) as a cross-linker, and the molar ratio of EPI to $\beta$-CD was 10 : 1. The $\beta$-CD content in $\beta$-CD polymers is about 52%. In order to get the photo-responsible and pH-responsible, cinnamic acid was added to be inserted into the cavities of $\beta$-CD due to the hydrophobic interaction. The complex formation was confirmed using transmission electron microscope. The dimerization degree of complexes increased under UV irradiation at $\lambda$ = 365 nm but decreased under the UV irradiation at $\lambda$ = 254 nm. Dynamic light scattering analysis of particle sizes showed that the sizes of complexes did not change with different UV wavelength. Moreover, the complexes were pH-responsible because of the carboxyl group of cinnamic acid, but the size and zeta potential of the complex did not change in strong acid and alkali conditions.

• The Journal of the Korea Contents Association
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• v.15 no.2
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• pp.363-369
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• 2015

Chronic pain is one of the leading causes of hospital visits. It not only affects the patients themselves but also has a major negative impact on their families and society. In this study, we investigated epidemiology of musculoskeletal disorders induced chronic pain among general population based on Korea National Health and Nutrition Examination Survey database and also analysed how it influenced on the use of medical services. This study was done by using the data of 5th Korea National Health and Nutrition Examination Survey (KNHANES V), taking aged 20 years and over adults as research subjects. The EuroQoL-5 Dimension Index(EQ-5D) was used as a survey instrument. T-test, chi-square test and multivariate logistic regression were used for statistical analysis. Subjects with chronic pain had a higher likelihood than control group to use medical services(odds ratio : 5.858, confidence interval 3.636-9.438). Controlling for existence of chronic pain, more women were likely to use medical services than men(1.156, 0.707-1.889). Age, gender and household income level did not affect the use of medical services. Proper control of chronic pain is very helpful in improving patient's quality of life and it also accounts for a large proportion in suppressing excessive consumption of medical services. Anesthesia and pain medicine specialists have superior knowledge about analgesics and anticonvulsants than other physicians do and also have specialized skills to perform procedures like nerve blocks in treating chronic pain. Therefore Anesthesia and pain medicine specialists need to play a leading role in managing chronic pain.

• The Korean Journal of Ecology
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• v.27 no.6 s.122
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• pp.335-345
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• 2004

This study was carried out to on liana dominating at the vicinity of Onsan Industrial Complex declining P. thunbergii foersts. It was surveyed 16 species, 30 species and 50 species at upper, middle and understory, respectively. It was dominant P. thunbergii at upperstory, and P. densiflora, P. thunbergii, Quercus dentata and Q. serrata at middlestory, and Smilax china, Paederia scandens, Symplocos chinensis var. sinuata, Styrax japonica, Lonicera japonica and Q. serrata etc. at understory. It was the highest importance value(13.2) of S. china among all liana, and in order of L. japonica (11.7), P. scandens (11.5) and Cocculus trilobus (7.7). Number of species, Species diversity, Maximum species diversity, Evenness and Dominance of woody plants at upperstory and middlestory by each survey site were higher in forest areas than industrial complex, but those of understory highly showed at industrial complex, and ratio of liana at understory was high. It showed total 50 species at herbaceous plants, and was high I.V. of Pteridium aquilinum var. latiusculum, Spodiopogon cotulifer, Commerelina commuris, Phytolacca americana, Rubus parvifolius, Miscanthussinensis var. purpurascens and Calamagrostis arundinacea, etc.

• Korean Journal of Optics and Photonics
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• v.11 no.2
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• pp.73-79
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• 2000

We measure a beam diameter of scan and sub-scan direction of LSD (Laser Scanning Urnt) which uses $fheta$ lens produced by injecLion molding method as a scanning lens. While the measured beam diameter in scan direction, which is $62muextrm{m}$ to $68\mu\textrm{m}$, shows similar size comparing to the design beam diameter, the sub-scan beam diameter shows sIzable beam diameter deviation as much as 37 11m ranging from $78\mu\textrm{m}$ to $115\mu\textrm{m}$. Injection molding lens has the surface figure error due to the shrinkage III the cooling time and the internal distortion (birefringence) due to the uneven cooling conditIOn so that these bring about wavefront aberration (i.e., the enlargement of beam size), and are eventually expre~sed as the deterioration of the pdnting image. In this paper. we first measure and analyze beam diameter, birefringence (polanzation ratio), and asphedcal figure error of mIens in order to know the principle cause of the beam diameter deviation in sub-scan directIOn. And Lhen. through the analysis of a designed depth of focus and a calculated field curvature (imaging position of the optical axis directIon) using the above figure elTor data, we know Lhat the birefringence IS the main factor of sizable beam diameter deVIation in sub-scan direction. ction.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.20 no.6
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• pp.157-165
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• 2020

Aiming at the development of neural network and self-driving data set, it is also an idea to improve the performance of network model to detect moving objects by dividing the data set. In Darknet network framework, the YOLOv4 (You Only Look Once v4) network model was used to train and test Udacity data set. According to 7 proportions of the Udacity data set, it was divided into three subsets including training set, validation set and test set. K-means++ algorithm was used to conduct dimensional clustering of object boxes in 7 groups. By adjusting the super parameters of YOLOv4 network for training, Optimal model parameters for 7 groups were obtained respectively. These model parameters were used to detect and compare 7 test sets respectively. The experimental results showed that YOLOv4 can effectively detect the large, medium and small moving objects represented by Truck, Car and Pedestrian in the Udacity data set. When the ratio of training set, validation set and test set is 7:1.5:1.5, the optimal model parameters of the YOLOv4 have highest detection performance. The values show mAP50 reaching 80.89%, mAP75 reaching 47.08%, and the detection speed reaching 10.56 FPS.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.32 no.6
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• pp.561-568
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• 2020

Wave measurements using X-band radar have many advantages compared to other wave gauges including wave-rider buoy, P-u-v gauge and Acoustic Doppler Current Profiler (ADCP), etc.. For example, radar system has no risk of loss/damage in bad weather conditions, low maintenance cost, and provides spatial distribution of waves from deep to shallow water. This paper presents new methods for estimating significant wave heights of X-band marine radar images using Artificial Neural Network (ANN). We compared the time series of estimated significant wave heights (Hs) using various estimation methods, such as signal-to-noise ratio (${\sqrt{SNR}}$), both and ${\sqrt{SNR}}$ the peak period (TP), and ANN with 3 parameters (${\sqrt{SNR}}$, TP, and Rval > k). The estimated significant wave heights of the X-band images were compared with wave measurement using ADCP(AWC: Acoustic Wave and Current Profiler) at Hujeong Beach, Uljin, Korea. Estimation of Hs using ANN with 3 parameters (${\sqrt{SNR}}$, TP, and Rval > k) yields best result.

• Tribology and Lubricants
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• v.37 no.6
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• pp.232-239
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• 2021

Petroleum is the most consumed energy source in Korea with a usage rate of 38.7% among the available primary energy sources. The price of liquid petroleum products in Korea includes taxes such as transportation·environment·energy tax. Thus, illegal production and distribution of liquid petroleum is widespread because of its huge price difference from that of the normal product and its tax-free nature. Generally, the illegal petroleum product is produced by mixing liquid petroleum with other similar petroleum alternatives. The two kinds of gasoline, common gasoline and premium gasoline, are being distributed in Korea. The premium gasoline is often adulterated with cheaper common gasoline that lowers the octane number of gasoline. It is possible to distinguish them with their color difference, green and yellow for different grade gasoline. However, when small volume of common gasoline is added to premium gasoline, it is difficult to determine whether premium gasoline contained common grade or not. In this study, we inspect gasoline, which is illegally produced by mixing common gasoline to premium gasoline. When the ratio of mixing common gasoline is increased, premium gasoline shows decreasing absorbance at 600 nm and 650 nm under UV-Vis spectrometer. Moreover, the detected intensity (mV·s) of green dye in high performance liquid chromatography (HPLC) was decreased by common gasoline under 0.99 correlation value. The more the common gasoline is mixed, the more olefin and naphthene are detected by gas chromatography. In addition, trimethyl pentane as octane improver, paraffin and toluene are decreased by common gasoline mixing. The findings of this study suggests that illegal petroleum can be identified by analysis of components and simulated samples.