• 대한환경공학회지
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• 제32권4호
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• pp.369-378
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• 2010

폐기물매립장의 안정화에는 미생물이 중요한 역할을 수행한다. 폐기물매립장에서 미생물군집 변화 모니터링에 말단 제한절편다형성(Terminal Restriction Fragment Length Polymorphism; T-RFLP)법의 활용 가능성을 평가하고자 박테리아의 16S rDNA 서열에 기초한 T-RFLP법으로 4개의 폐기물매립장 내부에서 채취한 침출수의 미생물군집 구조를 조사하였다. T-RFLP법을 사용하여 해석한 침출수 내 우점 미생물군집 구조와 일반적으로 널리 사용되고 있는 16S rDNA 클론 해석법에 의한 우점 미생물군집구조는 유사하였다. 또한, T-RFLP법을 이용하여 폐기물매립장의 구조, 매립 폐기물 종류, 운영기간이 다른 폐기물매립장 침출수의 우점 미생물군집 구조가 서로 다르게 나타나는 것을 확인 할 수 있었다. 따라서 T-RFLP법을 사용하여 폐기물매립장 침출수내 미생물군집 구조를 장기적으로 모니터링 한다면 많은 비용과 시간이 소요되는 클론해석법의 반복적인 수행 없이도 비교적 간단하게 폐기물매립장의 안정화 정도를 평가할 수 있을 것으로 기대한다.

• 통합자연과학논문집
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• 제7권1호
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• pp.50-56
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• 2014

To explore effects on each the emotional awareness, emotional expression, emotional empathy, and emotional regulation, of the sub-factors of the child's emotional intelligence, to mural preference, emotion cultivation, and community connection, the hierarchical multiple regression analyses are performed(as in Table 1, 2, and 3). As the results, we found the following facts. Children's mural preference, emotion cultivation, and community connection were expressed by the following equations in order, respectively. Mural Preference = $.170{\times}$[Emotional Awareness](t=2.118, $p=.036^*$) - $.025{\times}$[Emotional Expression](t=-.275, p=.783) + $.088{\times}$[Emotional Empathy](t=.938, p=.350) + $.139{\times}$[Emotional Regulation] (t=1.529, p=.128). Mural Emotion Cultivation = $-.021{\times}$[Emotional Awareness](t=-.294, p=.769) - $.205{\times}$[Emotional Expression](t=-2.573, $p=.011^*$) + $.265{\times}$[Emotional Empathy](t=3.156, $p=.002^*$) + $.192{\times}$[Emotional Regulation](t=2.361, $p=.019^*$). Mural Community Connection = $-.001{\times}$[Emotional Awareness](t=-.007, p=.995) - $.132{\times}$[Emotional Expression](t=-1.478, p=.141) + $.172{\times}$[Emotional Empathy](t=1.732, $p=.027^*$) + $.098{\times}$[Emotional Regulation](t=1.072, p=.285).

• 한국미생물학회:학술대회논문집
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• 한국미생물학회 2002년도 추계학술대회
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• pp.15-21
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• 2002

Terminal-restriction fragment length polymorphism (T-RFLP) analysis has been optimized by using in vitro model community composed of genomic DNAs of known bacterial strains and has been applied to assess the bacterial community structure in marine sediments. The specific fluorescence-labeled terminal restriction fragments (T-RFs) between 39 and 839 base long specifying each strain were precisely measured for known bacterial strains. The addition of a co-solvent (dimethylsulfoxide or glycerol) into PCR reactions has reduced differential PCR amplification. Comparative bacterial community structure was investigated for pristine and polluted sediments. A complex T-RFLP pattern showing complex bacterial community structure was obtained in the pristine sediment, whereas simple T-RFLP pattern (low bacterial diversity) was shown in polluted sediments where caged aquaculture has been conducted for several years. The results of T-RFLP analysis were compared with that of cloning and sequencing 16S rDNA clones from the same sediments. Sequence analysis of 16S rDNA clones (72) of the pristine sediment revealed a diverse collection of lineages, largely of the class Proteobacteria ($6\%$ alpha subdivision, $46\%$ gamma subdivision, $13\%$ delta subdivision, and $3\%$ epsilon subdivision), Nitrospina $(8\%)$, high G+C gram positive $(8\%)$, Verrucomicrobia $(7\%)$, and Planctomycetes $(6\%)$. In the contaminated sediments, 17 $(59\%)$ of the 16S rDNA clones (29) were related to Campylobacter and symbiont of Rimicaris exoculata belonging to epsilon subdivision of Proteobacteria. The results obtained indicated that T-RFLP analysis is a rapid and precise technique for comparative bacterial community analysis.

• 한국토양비료학회지
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• 제44권1호
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• pp.127-145
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• 2011

Various environmental ecosystems are valuable sources for microbial ecology studies, and their analyses using recently developed molecular ecological approaches have drawn significant attention within the scientific community. Changes in the microbial community structures due to various anthropogenic activities can be evaluated by various culture-independent methods e.g. ARISA, DGGE, SSCP, T-RFLP, clone library, pyrosequencing, etc. Direct amplification of total community DNA and amplification of most conserved region (16S rRNA) are common initial steps, followed by either fingerprinting or sequencing analysis. Fingerprinting methods are relatively quicker than sequencing analysis in evaluating the changes in the microbial community. Being an efficient, sensitive and time- and cost effective method, T-RFLP is regularly used by many researchers to access the microbial diversity. Among various fingerprinting methods T-RFLP became an important tool in studying the microbial community structure because of its sensitivity and reproducibility. In this present review, we will discuss the important developments in T-RFLP methodology to distinguish the total microbial diversity and community composition in the various ecosystems.

• 응용통계연구
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• 제29권4호
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• pp.719-728
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• 2016

본 연구에서는 한국과 미국의 사물 인터넷 관련 특허 초록을 수집하여 키워드 네트워크 및 키워드 커뮤니티 네트워크를 구축하고 네트워크 분석을 실시하였다. 먼저 TF-IDF 가중치를 적용하여 중요 키워드를 추출하고 이 중요 키워드와 상관관계가 높은 키워드들을 재추출하여 핵심 키워드를 선정하였다. 선정된 키워드를 중심으로 키워드 네트워크를 구축한 다음 네트워크 탐지를 시행하여 키워드 커뮤니티 네트워크를 재구축하여 기술 간의 연결 관계를 분석하였다. 본 연구에서 생성한 키워드 커뮤니티 네트워크는 특허의 내용을 예측할 수 있을 뿐만 아니라 커뮤니티 간의 연결 관계를 분석함으로써 기술 간의 연관 관계도 파악할 수 있다. 키워드 커뮤니티 네트워크 분석 결과 한국은 보안, 반도체, 이미지 프로세스와 같은 사물 인터넷의 기반 기술 분야의 특허가 중요한 특허 기술로 나타난 반면 미국의 경우 스마트 홈, 대화형 매체 그리고 통신 등과 같은 사물 인터넷 환경, 응용 분야의 기술이 중요한 기술로서 자리잡고 있음을 알 수 있다.

• Journal of Plant Biology
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• 제29권1호
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• pp.53-65
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• 1986

The montane forests of Ulreung Island, Korea, were investigated by the ZM school method. By comparing the montane forests of this island with those of Korean Peninsula and of Japan, a new order, F a g e t a l i a m u l t i n e r v i s, a new alliance, F a l g i o n m u l t i n e r v i s, a new association, H e p a t i c o-F a g e t u m m u l t i n e r v i s and Rhododendron brachycarpum-Pinus parviflora community were recognized. The H e p a t i c o - F a g e t u m m u l t i n e r v i s was further subdivided into four subassociations; Subass. of Sasa kurilensis, Subass. of Rumohra standishii, Subass. of Rhododendron brachycarpum and Subass. of typicum. Each community was described in terms of floristic, structural and environmental features.

• International journal of advanced smart convergence
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• 제7권3호
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• pp.44-60
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• 2018

This study is to identify the effects of hypertension management program at a community health center on the disease-related knowledge, self-efficacy, anthropometric measurements and blood pressure of a hypertensive patient in local community. This study is a quasi-experimental study using nonequivalence control group no-synchronized design in order to verify the effects of the hypertension management program at a community health center on the hypertension-related knowledge, self-efficacy, anthropometric measurements and blood pressure of a hypertensive patient in local community. The result indicated a significant difference between the experimental group and control group in the scores of hypertension-related knowledge (t=-4.25, p<.001), self-efficacy (t=-4.20, p<.001), systolic blood pressure (t=7.70, p<.001) and diastolic blood(t=5.91, p<.001), body weight(t=2.32, p=.026) and abdominal circumference(t=2.17, p=.036). The hypertensive patients' knowledge and self-efficacy were improved, and their weight and abdominal circumference as well as systolic blood pressure and diastolic blood pressure were reduced. Therefore, it was confirmed that multilateral approaches in terms of physical and psychosocial aspects only targeting hypertensive patients were required for managing hypertensive patients in local community.

• 한국환경생태학회지
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• 제29권2호
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• pp.250-262
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• 2015

This study aims to investigate the characteristics of the vegetation structure in the sectin stretching between the Jilmoi wetlands and the Donghae Observatory and to set the criteria for the basic data for a management plan including restoration afterwards. 12 plots($10m{\times}40m$, $20m{\times}20m$) were set up to analyse the vegetation structure. The analysis of the classification by TWINSPAN and ordination by DCA, importance percentage and property, distribution of diameter of breast height, growth increments of major woody species, species diversity and the physicochemical properties of soil were all analyzed. Vegetation classes were divided into 3 communities, which are community I (Pinus densiflora community), community II (Quercus mongolica community) and community III (Quercus mongolica-Tilia amurensis community). The P. densiflora community declined when competing with Q. mongolica and Fraxinus rhynchophylla and Q. mongolica competed with T. amurensis on an understory layer in Q. mongolica community. Q. mongolica competed with T. amurensis on both canopy and understory layers in Q. mongolica-T. amurensis community. P. densiflora declined and it was assumed to succeed to F. rhynchophylla or T. amurensis through Q. mongolica based on the importance percentage and distribution of the diameter of the breast height of small and middle sized trees. The age of P. densiflora was between 47 to 51 years old and Q. mongolica was 61years old. T. amurensis was 61 years old and the growth of Q. mongolica slowed a little. As the result of Shannon's index of species diversity, community I ranged from 0.9578 to 1.1862, community II ranged from 0.7904 to 1.2286 and community III ranged from 0.8701 to 1.0323. The contents of organic matter and cation were low compared to uncultivated mountain soil and it were analysed to be inappropriate for tree growth.

• 과학기술학연구
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• 제6권2호
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• pp.77-102
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• 2006

이 연구는 2002년 이후 이공계 기피 현상에 대한 논의가 진행되는 과정에 참여한 과학기술자들의 현실인식 및 정책 방안 제안 내용을 비교 분석한다. 이를 통해 우리나라 과학기술자 사회가 서로 다른 관심사와 이해관계를 가진 소집단들로 이루어졌고, 소집단을 가르는 기준에서 전공, 자격, 직무 못지 않게 과학기술자들의 소속기관이 중요하다고 주장한다. 또 이를 근거로 현재로서는 우리나라 과학기술자 사회가 구성원들의 이해와 관심을 잘 대표하고 공통의 이해관계와 관심사를 가진 실체를 형성하지는 못한 단계라고 평가한다. 이공계 기피 현상에 대한 논의는 대외 발언을 꺼리는 관행을 가진 과학기술자들이 대거, 자발적으로 자신들의 존재, 현실인식, 그리고 요구 사항을 공개적으로 드러내도록 만들었다. 이를 통해 과학기술자들은 존재를 주목받고 그들의 집단적 존재를 인정받은 측면이 있다. 그러나 동시에 그들이 서로 얼마나 다른 과학기술자인가를 보여주기도 했다. 그들의 현실 인식은 각자가 처한 환경 조건을 강하게 반영했으며 공통점보다는 차이점이 두드러졌기 때문이다. 우리나라 과학기술자 사회의 특성에 대한 기존 연구는 많지 않지만, 최근에 역사, 사회학, 정책 분야에서 관심을 가지고 연구가 진행되고 있다. 이러한 연구를 보면 과학기술자에 대한 규정을 다르게 하고 있음에도 불구하구 일단 과학기술자를 규정한 뒤에는 그들이 마치 단일한 특성을 가진 사회를 형성하는 것처럼 전제하고 분석한다. 그 결과 과학기술자 사회 또는 과학기술자 집단 구성원의 다양성은 인정되지만 집단으로서 그들의 차이점은 간과되거나 단순화되어 버리고 마침내 과학기술자 사회에 대해 잘못된 이해를 가질 수도 있게 된다.

• 농촌계획
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• 제19권1호
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• pp.33-41
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• 2013

Community building has been carried out from around the year 2000 by various statutes and government policies in Korea. This study suggested direction of the future for rural community building as surveyed cases of completed four villages in Gurye-county. In order to achieve this study's objectives, investigated previous studies and interviewed 75 person's residents in cases villagers. The target villages are Sangsa, Omi, Chamsaimigol and Artists village. As survey results, four villages utilized local resources, most of villages were focused perform hardware. On the other hand, Chamsaimigol was parallel software. In addition, communities didn't form and not agree with concept of community building as culture, education, welfare, etc., also didn't creation the case of village income. but active village as base development. Therefor, proposed the point to education, culture and welfare, base development and generating of income as directions for future rural community building. These directions are consistent with the concept of community building and the goal that improve the quality of life of residents ultimately through community building.