• Journal of The Korean Society of Grassland and Forage Science
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• v.17 no.4
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• pp.329-344
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• 1997

To improve germination and growth of forages of hill pasture seeds of several forages were osmotically primed with polyethylene glycol (PEG) under different PEG concentrations, treatment periods, and temperatures. Seeds were coated with several materials, and tested for germination. The best primed or coated seeds in germination tests were surface sow on hilly area. The results obtained are summarized as follows; 1. Osmotic priming with PEG accelerated germination of forages and reduced time taken for germination. 2. Germinability was best when the seeds of Tama ryegrass or orchardgrass were treated for 6-9 days at 30g PEG1100 ml water, red clover or white clover for 3, or 9 days in 20g PEG1100 ml water at $10^{\circ}C$. 3. Priming seeds of Tama ryegrass or orchardgrass in 30% PEG solution for 6 days, and seeds of red clover or white clover in 20% PEG solution for 6 days at $15^{\circ}C$ were most effective in germination, similar to priming at $10^{\circ}C$ . 4. Priming seeds of Tama ryegrass at 15C, and orchardgrass, red clover, or white clover at $10^{\circ}C$ were effective in germination than priming at other temperatures. 5. Osmotic priming with PEG accelerated germination of forages compared to coated seeds in Petri-dishes, while coated seeds germinated more slowly, but showed better emergence and superior growth to those of primed or intact seeds in the field. 6. Priming seeds increased yield slightly, and coating seeds significantly increased its yield on hilly area.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.1 no.1
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• pp.102-109
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• 1998

This study was conducted to find out the growth characteristic of mixed-seeding in the grasses for slope revegetation measures. Seeding treatment with six combination types of five grasses used for this experiment such as orchardgrass(Dactylis glomerata 'Potomac'), perennial ryegrass(Lolium perenne 'Manhattan'), tall fescue(Festuca arundinacea 'Jaguar'), kentucky bluegrass(Poa pratensis 'Majestic') and weeping lovegrass(Eragrostis curvula). The growth of orchardgrass, perennial ryegrass and tall fescue were good on mixed-seeding condition in case of number of individuals, heights and coverages at this experiment. These plants will useful for revegetation measures mixed-seeding, but weeping love grass and kentucky bluegrass did not show a desirable growth in mixed-seeding condition, and these plant prefered mixed seeding to single seeding. Regeneration percents of orchardgrass, perennial ryegrass and tall fescue were higher than that of weeping love grass and kentucky bluegrass, and coverage kept on good condition through one year after seeding, therefore, these plants will effective for initial stage revegetation measures.

• Journal of The Korean Society of Grassland and Forage Science
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• v.39 no.3
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• pp.127-131
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• 2019

The effects of lactic acid bacteria (LAB) mixtures on low moisture Italian ryegrass (IRG) silage fermentation was evaluated in field conditions. The experiment was categorized into two groups: Un-inoculated (Control) and Inoculated with LAB mixture for four storage periods (45, 90, 180, and 365 days, respectively). Silage inoculated with the LAB mixture had the lowest pH with highest lactic acid production than the control from beginning at 45-365 days at all moistures. Higher LAB counts were observed in inoculated silages than the control silages at whole experimental periods. It is a key reason for the rapid acidification and higher lactic acid production in silages during the storage periods. Overall results suggest that an adding of LAB mixture had positive effects on the increasing aerobic stability of silage and preserved its quality for an extended duration.

• Journal of The Korean Society of Grassland and Forage Science
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• v.39 no.3
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• pp.158-164
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• 2019

We examined the adaptability of Korean winter forage crops such as Italian ryegrass, barley, oat, and triticale in Uzbekistan. The overall growth before wintering was slightly different between Uzbekistani and Korean varieties, but there was no significant difference among winter forage crops. The heading date of the Korean triticale 'Shinyoung' was the earliest, (April 4), and the heading date of the Uzbekistani triticale 'Frack cerebristy' was quite late, (April 25). The heading date of the Korean Italian ryegrass 'Kowinearly' was four days earlier than that of 'Kowinmaster,' on April 13, and that of the Korean barley 'Youngyang' and 'Yuyeon' were five days earlier than that of the Uzbekistani barley 'Bolgaly'. The maximum dry matter yield of winter forage crop was from Uzbekistan triticale, 'Frack cerebristy', which was 13,536 kg/ha. The dry matter yield of Uzbekistan barley was 8,222 kg/ha. Compared with Uzbekistan barley, 'Bolgaly', yields of Korean barley 'Youngyang' and 'Yuyeon', Korean oat 'Samhan', and Korean Italian ryegrass 'Kowinearly' and 'Kowinmaster' were 59~64%, 125%, and 113~133%, respectively.

• Journal of The Korean Society of Grassland and Forage Science
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• v.42 no.3
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• pp.208-214
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• 2022

Drought stress is a condition that occurs frequently in the field, it reduces of the agricultural yield of field crops. The aim of the study was to screen drought-adapted genotype of Italian rye grass. The experiments were conducted between the two Italian ryegrass (Lolium multiflorum L.) cultivars viz. Hwasan (H) and Kowinearly (KE). The plants were exposed to drought for 14 days. The results suggest that the morphological traits and biomass yield of KE significantly affected by drought stress-induced oxidative stress as the hydrogen peroxide (H₂O₂) level was induced, while these parameters were unchanged or less affected in H. Furthermore, the cultivar H showed better adaptation by maintaining several physiological parameter including photosystem-II (Fv/Fm), water use efficiency (WUE) and relative water content (RWC%) level in response to drought stress. These results indicate that the cultivar H shows improved drought tolerance by generic variation, improving photosynthetic efficiency and reducing oxidative stress damages under drought stress. These findings can be useful to the breeder and farmer for improving drought tolerance in Italian rye grass through breeding programs.

• Korean Journal of Agricultural Science
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• v.45 no.1
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• pp.84-93
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• 2018

This study was conducted to develop a pasture feeding program for reducing the Hanwoo feeding cost. Italian ryegrass (IRG) is a major plant which is abundantly produced in the southern area of South Korea. Locally produced Italian ryegrass has a 30 - 50% reduced pasture cost compared to imported pasture grasses. We hypothesized that Italian ryegrass feed could reduce the feeding cost but not change the physiological characteristics of the Hanwoo steers in the feedlot. Four treatments (Control, short fed IRG silage, long fed IRG silage, and IRG hay) in a completely random design were used to feed 27 Hanwoo steers. Three or four steers were fed in the same pen, and 8 pens were used for the treatments. Blood was drawn from each steer on the first week of every month from 11 to 30 months. Overall, the average daily gain and feed efficiency were not different among the treatments (p < 0.05) except for the final fattening diets (p < 0.05). The long fed IRG silage group had a low ADG compared to the other groups (p < 0.05). The serum non-esterified fatty acid and glucose concentrations were reduced during the fattening periods (p < 0.05); however, the serum albumin, total proteins, triglycerides, and phosphorus were increased during the fattening periods (p < 0.05). The carcass weight, longissimus muscle area and back-fat thickness were low in the long fed IRG silage group (p < 0.05). The IRG diet induced saturated fatty acids in the longissimus dorsi (p < 0.05). These results show that the IRG diet increased the serum triglycerides but reduced the glucose level at the final fattening diet and decreased the meat yield score in Hanwoo steers.

• Asian-Australasian Journal of Animal Sciences
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• v.30 no.6
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• pp.819-827
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• 2017

Objective: Four lactic acid bacteria (LAB) strains isolated from common vetch, tall fescue and perennial ryegrass on the Tibetan Plateau were characterized, and their effects on the fermentation quality of Italian ryegrass (Lolium multiflorum Lam.) silage were studied. Methods: The four isolated strains and one commercial inoculant (G, Lactobacillus plantarum MTD-1) were evaluated using the acid production ability test, morphological observation, Gram staining, physiological, biochemical and acid tolerance tests. The five LAB strains were added to Italian ryegrass for ensiling at three different temperatures ($10^{\circ}C$, $15^{\circ}C$, and $25^{\circ}C$). Results: All isolated strains (LCG3, LTG7, I5, and LI3) could grow at $5^{\circ}C$ to $20^{\circ}C$, pH 3.0 to 8.0 and NaCl (3.0%, 6.5%). Strains LCG3, LTG7, I5, and LI3 were identified as Lactobacillus plantarum, Pediococcus acidilactici, Lactobacillus paraplantarum, and Lactobacillus casei by sequencing 16S rDNA, respectively. All LAB inoculants significantly (p<0.05) increased lactic acid (LA) contents and ratios of lactic acid to acetic acid, and reduced pH and ammonia nitrogen/total nitrogen (AN/TN) compared with uninoculated silages at various temperatures ($10^{\circ}C$, $15^{\circ}C$, and $25^{\circ}C$). Compared to the commercial inoculant G, I5, and LI3 showed similar effects on improving the silage quality of Italian ryegrass at $10^{\circ}C$ and $15^{\circ}C$, indicated by similar pH, LA content and AN/TN. Conclusion: All inoculants could improve the silage fermentation quality at various temperatures ($10^{\circ}C$, $15^{\circ}C$, and $25^{\circ}C$). At the temperature of $10^{\circ}C$ and $15^{\circ}C$, strain I5 and LI3 had similar effects with the commercial inoculant G on improving the silage quality of Italian ryegrass.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.1
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• pp.104-111
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• 1997

Since seeds of Italian ryegrass should be imported every year, it is necessary to investigate the prossible production of seeds in Italian ryegrass field after rice. Seven Italian ryegrass varieties were planted on October 8, 1986 at National Honam Crop Experiment Station at Iksan and clippings were made on April 10 and April 30 in 1987. Headings of K-11 and T.N.T were earlier than those of Tetrone and Bettina. For all varieties tested, heading date was delayed and 1,000 grain weight was decreased linearly as the clipping was made later. Length, width and thickness of a grain were reduced by clipping and later clipping, while tetraploid varieties produced larger grains than diploid varieties. The germination percentage and germination speed at $25^{\circ}C$ were higher than at 15$^{\circ}C$. The final germination percentage was lower at the later clippings and was higher at 15$^{\circ}C$, and earlier varieties showed higher germination percentage. Uniformity of germination was greater at $25^{\circ}C$ and was decreased as the clipping was made later, and the eariler varieties such as K-11 were germinated more uniformly. Average number of days to germination was longer at 15$^{\circ}C$ than at $25^{\circ}C$ and was longer at later clipping. The germination percentage was decreased linearly as the heading was delayed, while it increased linearly as the 1,000 grain weight was increased.

• Journal of Animal Science and Technology
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• v.46 no.3
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• pp.443-450
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• 2004

To examine the potentiality of grassland dominated with turf-type grasses, which are mainly used for the establishment of green spaces, forage production and its utilization as forage resources, dry matter yield and forage quality of three different turf-type mixtures were estimated throughout three consecutive experimental years. According to the experiment, the mean of dry matter(DM) yield, chemical composition, DM digestibility, crude protein dry matter(CPDM) and digestible dry matter(DDM) yield was not significantly different among species(p > 0.05) over three experimental years. Botanical composition of three dominant species(tall fescue, Kentucky bluegrass and perennial ryegrass) was 42, 35 and 28%, respectively, in three mixtures at the last cutting(6th) in 2001. The botanical composition of three dominated species such as tall fescue, Kentucky bluegrass and perennial ryegrass was maintained 84% of the total plants in all types of mixtures. Therefore, It is observed that the mixture types did not closely affect the botanical composition. But botanical composition of the other species such as redtop(8%) and creeping bentgrass(6${\sim}$7%) was low. Especially, that of red fescue was the least(1${\sim}$2%) of all mixed species. As mentioned above, any difference was not observed in DM yields, forage quality and botanical composition among mixture types. Therefore, any types of mixtures can be used as public green spaces like school ground areas or river-sides, and as forage resources additionally.

• Journal of The Korean Society of Grassland and Forage Science
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• v.29 no.3
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• pp.165-170
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• 2009

A system for the production of transgenic plants has been developed for perennial ryegrass (Lolium perenne L.) via Agrobacterium-mediated transformation. Included in this study were two factors which may affect the gene transfer efficiency: concentrations of acetosyringone (AS, 0 to 300 ${\mu}M$), and co-culture period (1 to 7 days). Both factors were very important to achieve high efficiency gene transformation in the perennial ryegrass. The highest transformation efficiency was obtained when embryogenic calli were inoculated with Agrobacterium in the presence of 100 ${\mu}M$ AS with the culture medium for 5 days. Phosphinothricin resistant calli were developed with into complete plants. GUS histochemical assay, polymerase chain reaction (PCR) and Northern blot analysis of transgenic plants demonstrated that transgenes were integrated into the genome of perennial ryegrass. Using this protocol, it was possible to obtain transformants efficiently for further study.