• Journal of Plant Biotechnology
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• v.44 no.2
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• pp.149-155
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• 2017

Bacterial blight in rice caused by Xanthomonas oryzae pv. oryzae (Xoo) greatly reduces the growth and productivity of this important food crop. Therefore, we sought to increase the resistance of rice to bacterial blight by using a NAC (NAM, ATAF, and CUC) transcription factor, one of the plant-specific transcription factors that is known to be involved in biotic/abiotic stress resistance. By isolating the OsNAC58 gene from rice and analyzing its biological functions related to Xoo resistance, phylogenetic analysis showed that OsNAC58 belongs to group III. To investigate the biological relationship between bacterial leaf blight (BLB) and OsNAC58 in rice, we constructed a vector for overexpression in rice and generated transgenic rice. The expression analysis resulting from use of RT-PCR showed that OsNAC58-overexpressed transgenic rice exhibited higher levels of OsNAC58 expression than wild types. Further, subcellular localization analysis using rice protoplasts showed that the 35S/OsNAC58-SmGFP fusion protein was localized in the nuclei. Thirteen OsNAC58-overexpressed transgenic rice lines, with high expression levels of OsNAC58, showed more resistant to Xoo than did the wild types. Together, these results suggest that the OsNAC58 gene of rice regulates the rice disease resistance mechanism in the nucleus upon invasion of the rice bacterial blight pathogen Xoo.

• Journal of the Korean Wood Science and Technology
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• v.44 no.4
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• pp.477-493
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• 2016

A single process using hot water (0% green liquor) and green liquor (GL) was investigated for pre-pulping extraction on two types of raw material. The GL was applied at different alkali charges of 0-5% on a dry wood weight basis. The extractions were performed at an H-factor 900 at $180^{\circ}C$. The 0% and 3% GL extraction detected acetic acid (AA) at 10.02 and $9.94g/{\ell}$, extracts derived from hardwood, 2.46 and $3.76g/{\ell}$, extracts derived from softwood, respectively. The single liquid-liquid extraction (LLE) was studied using tri-n-alkylphosphine oxide (TAPO). Response surface methodology (RSM) was employed as an efficient approach for predictive model building and optimization of AA recovery conditions. The extraction of AA was evaluated with a three-level factorial design. Three independent variables, pH (0.5-3.5), temperature ($25-65^{\circ}C$), and residence time (24-48 min) were investigated. Applying the RSM models obtained, the optimal conditions selected of extracts derived from hard- and softwood with a 3% GL were approximately pH 1.4, $26.6^{\circ}C$, 43.8 min and approximately pH 0.7, $25.2^{\circ}C$, 24.6 min, respectively. The predicted and experimental values of AA recovery yield were similar whilst sugar retention was 100%.

• Journal of the Korean Society of Industry Convergence
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• v.22 no.2
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• pp.163-171
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• 2019

Recently, rural housing in urban areas has been increasing due to the improvement of income level. With the increase of the construction of the power house, the installation of the heating system using the harmonious fuel for the purpose of heating and the beauty of the room is increasing rapidly. In addition to the increasing use of firewood heating equipment, the incidence of fire is also increasing. Analysis of the National Fire Data System of the Fire Department The result of the analysis of the National Fire Data System Many parts of the fire are incinerated by the accumulation of tar due to the incompleteness of periodic cleaning inside the cylinder. The distance between the fire extinguisher and the combustible materials such as ceiling, Resulting in fire. In addition, it was found that much of the fire of the firewood heating system in the time zone occurs during the sleeping and resting time and there is not enough time for the residents to cope. This, in turn, causes serious harm to the lives and property of the users of the pyrotechnic heating system. Therefore, in this study, domestic and foreign standards and laws related to fuel oil heating facilities were analyzed and 12 cases of fire accidents were analyzed. Through the revision of the fire prevention and firefighting facilities installation and maintenance law, the installation standards of the alarm and fire extinguishing facilities were presented.

• Journal of the Korean Society of Industry Convergence
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• v.26 no.6_2
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• pp.1135-1144
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• 2023

In this paper, bending strength and permeability tests were conducted on concrete permeable blocks manufactured by recycling industrial by-products of oyster shell and blast furnace slag to measure and compare bending strength and permeability coefficient, and present experimental research results. To this end, a total of 54 specimens with a size of 200x200x60mm for surface layer and base layer were manufactured, and bending strength and permeability test were carried ourt accoridng to KS F 4419. Eighteen types of mixing designs were implemented by varying the mixing and replacement rates of oyster shells and blast furnace slag. As a result of the experiment, the higher the mixing ratio of oyster shell, the lower the bending strength and the permeability coefficient. Thereafter, a total of three permeable blocks with dimensions of 200x200x60mm were manufactured and subjected to bending strength and permeability tests according to KS F 4419. As a result of the test, the bending strength satisfies the standard of KS F 4419, and the permeability coefficient is 12 times higher than the standard of KS F 4419. It seems that the proper mixing of oyster shells and blast furnace slag increases the amount of air, and further research on durability and economic feasibility of materials used to manufacture permeable blocks is required.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.22 no.3
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• pp.160-168
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• 2021

This study was undertaken to determine the effect of different cooling systems on the performance and hair cortisol of sows under heat stress. During a 21-day experiment, a total of 40 multiparous sows (Landrace×Yorkshire; 242.84±2.89 kg) were allotted to 4 treatments, each with 10 replicates (1 sow per pen). The experimental treatments were CP (Cooling pad), AC (Air conditioner), SC (Snout cooling), and MS (Mist spray). We observed an increase in the average daily feed intake during lactation (p<0.05) in the CP and AC treatment groups. AC treatment had the highest (p<0.05) and SC treatment had the lowest (p<0.05) piglet weight at weaning. During lactation, sows administered SC and MS treatments had higher (p<0.05) hair cortisol accumulation, as compared with the AC and CP treatments. Hair cortisol accumulation in piglets during lactation was highest with MS treatment (p<0.05), and lowest after CP treatment (p<0.05). MS treatment had the highest (p<0.05), and AC treatment had the lowest (p<0.05) respiratory rate and rectal temperature during lactation. In conclusion, our results indicate that a cooling pad and air conditioning cooling system increases the productivity of a sow, as compared to snout cooling and mist spray cooling systems.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.11
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• pp.376-385
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• 2019

This study was undertaken is to isolate brewing yeast suitable for rice beer fermentation from the traditional Nuruk, and to identify the brewing ability of the isolated yeast. After 6 months of research, four brewing yeast isolated from traditional Nuruk showed a normal fermentation pattern in terms of physicochemical data (pH, brix, alcohol content) and higher vitality, as compared to commercial brewing yeast. The concentrations of higher alcohol and ester, that impart the aroma to beer, were 78.4 to 106.5 ppm and 15.1 to 29.3 ppm, respectively. In particular, S. cerevisiae (KCCM 90313) bestowed significantly higher contents of higher alcohol and ester concentrations than rice beer prepared from commercial yeast. We conclude that the four variants of yeast isolated from traditional Nuruk are potentially suitable for manufacturing rice beer. Especially, the S. cevisiae (KCCM 90313) yeast shows excellent yeast activity and aroma production, thereby displaying potential application for manufacturing rice beer in the future.

• Journal of Animal Science and Technology
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• v.62 no.1
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• pp.31-42
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• 2020

The aim of this study was to investigate the effects of 3-nitrooxypropanol (NOP) on gas production, rumen fermentation, and animal performances depending on animal type using a meta-analysis approach. A database consisted of data from 14 studies, 18 experiments and 55 treatments. The supplementation of NOP linearly decreased methane (CH₄) emissions [g/kg dry matter intake (DMI)] regardless of animal type and length of experimental period (beef, p < 0.0001, R² = 0.797; dairy, p = 0.0003, R² = 0.916; and long term, p < 0.0001, R² = 0.910). The total volatile fatty acids (VFA) concentration and the proportion of acetate, based on beef cattle database, were significantly decreased with increasing NOP supplementation (p = 0.0015, R² = 0.804 and p = 0.0003, R² = 0.918), whereas other individual VFAs was increased. Based on the dairy database, increasing levels of NOP supplementation linearly decreased proportion of acetate (p = 0.0284, R² = 0.769) and increased that of valerate (p = 0.0340, R² = 0.522), regardless of significant change on other individual VFAs. In animal performances, the DMI, from beef cattle database, tended to decrease when the levels of NOP supplementation increased (p = 0.0574, R² = 0.170), whereas there was no significant change on DMI from dairy cattle database. The NOP supplementation tended to decrease milk yield (p = 0.0606, R² = 0.381) and increase milk fat and milk protein (p = 0.0861, R² = 0.321, p = 0.0838, R² = 0.322). NOP is a viable candidate as a feed additive because of its CH₄ mitigation effects, regardless of animal type and experiment period, without adverse effects on animal performances.

• Journal of Digital Convergence
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• v.20 no.4
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• pp.147-157
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• 2022

This study tried to propose a smart farm technology strategy suitable for the domestic situation, focusing on the differentiation suitable for the domestic situation of ICT technology. In the case of advanced countries in the overseas agricultural industry, it was confirmed that they focused on the development of a specific stage that reflected the geographical characteristics of each country, the characteristics of the agricultural industry, and the characteristics of the people's demand. Confirmed that no enemy development is being performed. Therefore, in response to problems such as a rapid decrease in the domestic rural population, aging population, loss of agricultural price competitiveness, increase in fallow land, and decrease in use rate of arable land, this study aims to develop smart farm ICT technology in the future to create quality agricultural products and have price competitiveness. It was suggested that the smart farm should be promoted by paying attention to the excellent performance, ease of use due to the aging of the labor force, and economic feasibility suitable for a small business scale. First, in terms of economic feasibility, the ICT technology is configured by selecting only the functions necessary for the small farm household (primary) business environment, and the smooth communication system with these is applied to the ICT technology to gradually update the functions required by the actual farmhouse. suggested that it may contribute to the reduction. Second, in terms of performance, it is suggested that the operation accuracy can be increased if attention is paid to improving the communication function of ICT, such as adjusting the difficulty of big data suitable for the aging population in Korea, using a language suitable for them, and setting an algorithm that reflects their prediction tendencies. Third, the level of ease of use. Smart farms based on ICT technology for the development of the Industry6.0 (1.0(Agriculture, Forestry) + 2.0(Agricultural and Water & Water Processing) + 3.0 (Service, Rural Experience, SCM)) perform operations according to specific commands, finally suggested that ease of use can be promoted by presetting and standardizing devices based on big data configuration customized for each regional environment.

• The Plant Pathology Journal
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• v.32 no.6
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• pp.552-562
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• 2016

Pathogenesis-related proteins play multiple roles in plant development and biotic and abiotic stress tolerance. Here, we characterize a rice defense related gene named "jasmonic acid inducible pathogenesis-related class 10" (JIOsPR10) to gain an insight into its functional properties. Semi-quantitative RT-PCR analysis showed up-regulation of JIOsPR10 under salt and drought stress conditions. Constitutive over-expression JIOsPR10 in rice promoted shoot and root development in transgenic plants, however, their productivity was unaltered. Further experiments exhibited that the transgenic plants showed reduced susceptibility to rice blast fungus, and enhanced salt and drought stress tolerance as compared to the wild type. A comparative proteomic profiling of wild type and transgenic plants showed that overexpression of JIOsPR10 led to the differential modulation of several proteins mainly related with oxidative stresses, carbohydrate metabolism, and plant defense. Taken together, our findings suggest that JIOsPR10 plays important roles in biotic and abiotic stresses tolerance probably by activation of stress related proteins.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2015.05a
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• pp.777-780
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• 2015

Currently, our country has promoted sustainable growth in agriculture field by expanding the growth engine which is going to creat new value through agrifood industry & ICT convergence, the deployment of computerization in rural areas and the efficiency increase of agricultural administration system. Since the level of domestic agriculture-ICT convergence technology focusing on production areas is at early stage, it is necessary to deploy the successful models through the systematic development of technology and standardization including production, distribution and consumption phase. In addition, because the management and control systems of large glass greenhouse are mostly foreign products with no standardization and related small domestic companies, there is a limit to agri-food & ICT convergence activation led by the agri-food private sector. Also, it is vital to increase productivity & efficiency and improve quality throughout the entire agricultural process including production, distribution and consumption by the fusion of information technology, automatic control technology and unique ICT on existing agricultural technology, Therefore, in this paper we propose the agricultural-ICT convergence technology fields in which our country can lead technology and the standardization plans through analyzing the development, policy and standardization trends on agricultural-ICT convergence technology.