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Characterisation of runs of homozygosity and inbreeding coefficients in the red-brown Korean native chickens

  • John Kariuki Macharia;Jaewon Kim;Minjun Kim;Eunjin Cho;Jean Pierre Munyaneza;Jun Heon Lee
    • Animal Bioscience
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    • v.37 no.8
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    • pp.1355-1366
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    • 2024
  • Objective: The analysis of runs of homozygosity (ROH) has been applied to assess the level of inbreeding and identify selection signatures in various livestock species. The objectives of this study were to characterize the ROH pattern, estimate the rate of inbreeding, and identify signatures of selection in the red-brown Korean native chickens. Methods: The Illumina 60K single nucleotide polymorphism chip data of 651 chickens was used in the analysis. Runs of homozygosity were analysed using the PLINK v1.9 software. Inbreeding coefficients were estimated using the GCTA software and their correlations were examined. Genomic regions with high levels of ROH were explored to identify selection signatures. Results: A total of 32,176 ROH segments were detected in this study. The majority of the ROH segments were shorter than 4 Mb. The average ROH inbreeding coefficients (FROH) varied with the length of ROH segments. The means of inbreeding coefficients calculated from different methods were also variable. The correlations between different inbreeding coefficients were positive and highly variable (r = 0.18-1). Five ROH islands harbouring important quantitative trait loci were identified. Conclusion: This study assessed the level of inbreeding and patterns of homozygosity in Red-brown native Korean chickens. The results of this study suggest that the level of recent inbreeding is low which indicates substantial progress in the conservation of red-brown Korean native chickens. Additionally, Candidate genomic regions associated with important production traits were detected in homozygous regions.

The Characteristics of Fish Fauna by Habitat Type and Population of Zacco platypus in the Hongcheon River (홍천강의 서식처 유형별 어류상과 피라미 개체군의 특징)

  • Lee, Hwang-Goo;Jang, Chang-Ryeol;Choi, Jun-Kil
    • Korean Journal of Environment and Ecology
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    • v.27 no.2
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    • pp.230-240
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    • 2013
  • The fish fauna and population characteristics of Zacco platypus at the 9 habitat types in the Hongcheon River were investigated from August, 2009 to April, 2010. The collected species during the surveyed period were 21 species belong to 6 families. Korea endemic species were Acheilognathus signifer, Microphysogobio longidorsalis, Pseudopungtungia tenuicorpa, Squalidus gracilis majimae, Zacco koreanus, Iksookimia koreensis, Silurus microdorsalis, Liobagrus andersoni, Coreoperca herzi, Odontobuitis interrupta, and Odontobuitis platycephala which showed 61.9% ratio of total species. Dominant species was Z. koreanus, and subdominant species was Z. platypus. Dominant species according to habitat types were Z. platypus(in side channel, substrate type pool, and riffle), Z. koreanus(channel connected pool, run, meander type pool, and rock type pool), M. yaluensis( dam type pool ) and Rhynchocypris oxycephalus(channel unconnected pool). Length-weight relationship in the population of Z. platypus was 3.27 in regression coefficient(b). Principal component analysis was classified as 2 groups. Bray-curtis cluster analysis indicated that the channel connected pool and meander type pool showed the most similar values(66.2%), whereas side channel and channel unconnected pool exhibited the most distance values(32.1%).

Habitat assessment of Lamprotula coreana by using physical habitat simulation system (PHABSIM) at the Guem River (Physical habitat simulation system (PHABSIM) 을 이용한 금강의 두드럭조개 (Lamprotula coreana) 서식지 평가)

  • Kim, Dae-Hee;Kim, Kyeong-Hwan;Lee, Won-Ok;Hur, Jun-Wook
    • The Korean Journal of Malacology
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    • v.31 no.4
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    • pp.307-314
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    • 2015
  • This study sampled endangered species, Lamprotula coreana, and surveyed its habitat at the Guem River with three times from June to August 2013. To assess the habitat, this study conducted field survey considering diverse physical conditions of stream, such as pool, run and riffle, and measured transect, water depth, water velocity, substrate structure, and habitat type. When L. coreana collected, length, weight and age were measured. Water velocity, water depth and substrate structure were recorded to develop HSI (habitat suitability index) and performed PHABSIM (physical habitat simulation) to estimate the optimum flow discharge. Water level, flow discharge and transect data were used for habitat assessment, and PHABSIM was applied to calculate WUA (weighed usable area). Shell length was $73.1{\pm}18.4mm$ (28.5-102.0 mm), shell weight was $131.6{\pm}72.3g$ (28.0-281.0 g) and age was two to seven years from L. coreana collected at the upstream of the Guem River. Developed HSI indicated that the optimal habitat for L. coreana was 0.4-0.5 m for water depth, 0.3-0.5 m/s for water velocity and sand to boulder for substrate structure. The optimum ecological flow discharge for L. coreana was 2.1 cms and WUA was $3,730m^2$/1000 m by the result of PHABSIM. Recently, river construction work and habitat disturbance have caused negative impact on the distribution of L. coreana. The result of this study would provide fundamental data for habitat restoration and management of L. coreana.

Physical Habitat Assessment of Pale Chub (Zacco platypus) to Stream Orders in the Geum River Basin (하천차수에 따른 금강수계 피라미(Zacco platypus)의 물리적 서식지 평가)

  • Hur, Jun-Wook;Park, Sang-Young;Kang, Shin-Uk;Kim, Jeong-Kon
    • Korean Journal of Environmental Biology
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    • v.27 no.4
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    • pp.397-405
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    • 2009
  • We assessed the physical habitat conditions of pale chub (Zacco platypus) considering various stream order in the Geum river basin. Field monitoring was conducted for ecological and habitat conditions for 28 sites from October 2007 to October 2008. The number of Z. platypus sampled during this period was 2,362. In more than 3rd stream, water depth and velocity showing the highest number of samples were 0.8 m and $0.8\;m\;s^{-1}$, respectively. In addition, the highest number of samples was observed under the condition of total length (TL) (8~10 cm), velocity ($0.3\;m\;s^{-1}$), water depth (0.4 m), substrate size (send, 0.1~1.0 mm~coarse gravel, 50.0~100.0 mm) and habitat type (run). Based on the monitoring data (n=1059) the relationship between body weight (BW) and TL in male and female were estimated as $BW=0.0068{\times}TL^{3.0274}$ ($r^2=0.9102$) and $BW=0.0075{\times}TL^{2.9995}$ ($r^2=0.8517$), respectively. Growth equations on days after parturition (DAP) to the TL were estimated as TL=0.0108DAP+1.5795 ($r^2=0.9721$). By equations (TL 6.9 cm), BW were 2.7 g (male) and 2.4 g (female), and DAP was about 490 days.

Skew Compensation and Text Extraction of The Traffic Sign in Natural Scenes (자연영상에서 교통 표지판의 기울기 보정 및 덱스트 추출)

  • Choi Gyu-Dam;Kim Sung-Dong;Choi Ki-Ho
    • The Journal of The Korea Institute of Intelligent Transport Systems
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    • v.3 no.2 s.5
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    • pp.19-28
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    • 2004
  • This paper shows how to compensate the skew from the traffic sign included in the natural image and extract the text. The research deals with the Process related to the array image. Ail the process comprises four steps. In the first fart we Perform the preprocessing and Canny edge extraction for the edge in the natural image. In the second pan we perform preprocessing and postprocessing for Hough Transform in order to extract the skewed angle. In the third part we remove the noise images and the complex lines, and then extract the candidate region using the features of the text. In the last part after performing the local binarization in the extracted candidate region, we demonstrate the text extraction by using the differences of the features which appeared between the tett and the non-text in order to select the unnecessary non-text. After carrying out an experiment with the natural image of 100 Pieces that includes the traffic sign. The research indicates a 82.54 percent extraction of the text and a 79.69 percent accuracy of the extraction, and this improved more accurate text extraction in comparison with the existing works such as the method using RLS(Run Length Smoothing) or Fourier Transform. Also this research shows a 94.5 percent extraction in respect of the extraction on the skewed angle. That improved a 26 percent, compared with the way used only Hough Transform. The research is applied to giving the information of the location regarding the walking aid system for the blind or the operation of a driverless vehicle

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The Fermentative Hydrogen Production in Trickling Bed Biofilter Filled with Hydrophilic-and Hydrophobic-Media (소수성 및 친수성 담체를 이용한 Trickling Bed Biofilter의 생물학적 수소생산)

  • Jeon, Byung-Seung;Lee, Sun-Mi;Kim, Yong-Hwan;Gu, Man-Bock;Chae, Hee-Jeong;Sang, Byoung-In
    • Transactions of the Korean hydrogen and new energy society
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    • v.17 no.4
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    • pp.379-388
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    • 2006
  • Two mesophilic trickling bed bioreactors filled with two different types of media, hydrophilic- and hydrophobic-cubes, were designed and conducted for hydrogen production under the anaerobic fermentation of sucrose. Each bioreactor consisted of the column packed with polymeric cubes and inoculated with heat-treated sludge obtained from anaerobic digestion tank. A defined medium containing sucrose was fed by the different hydraulic retention time(HRT), and recycle rate. Hydrogen concentrations in gas-phase were constant, averaging 40% of biogas throughout the operation. Hydrogen production rate was increased till $10.5\;L{\cdot}h^{-1}{\cdot}L^{-1}$ of bioreactor when influent sucrose concentrations and recycle rates were varied. At the same time, the hydrogen production rate with hydrophobic media application was higher than its hydrophilic media application. No methane was detected when the reactor was under a normal operation. The major fermentation by-products in the liquid effluent of the both trickling biofilters were acetate, butyrate and lactate. In order to run in the long term operation of both reactor filled with hydrophilic and hydrophobic media, biofilm accumulation on hydrophilic media and biogas produced should be controlled through some process such as periodical backwashing or gas-purging. Four sample were collected from each reactor on the opposite hydrogen production rate, and their bacterial communities were compared by terminal restriction fragment length polymorphism (T-RFLP) analysis of PCR products generated using bacterial 16s rRNA gene primers (8f and 926r). It was expressed a marked difference in bacterial communities of both reactors. The trickling bed bioreactor with hydrophobic media demonstrates the feasibility of the process to produce hydrogen gas. A likely application of this reactor technology can be hydrogen gas recovery from pre-treatment of high carbohydrate-containing wastewaters.

Investigation of Minimum Number of Drop Levels and Test Points for FWD Network-Level Testing Protocol in Iowa Department of Transportation (아이오와 주 교통국의 FWD 네트워크 레벨 조사 프로토콜을 위한 최소 하중 재하 수와 조사지점 수의 결정)

  • Kim, Yong-Joo;Lee, Ho-Sin(David);Omundson, Jason S.
    • International Journal of Highway Engineering
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    • v.12 no.4
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    • pp.39-46
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    • 2010
  • In 2007, Iowa department of transportation (DOT) initiated to run the falling weight deflectometer (FWD) network-level testing along Iowa highway and road systems and to build a comprehensive database of deflection data and subsequent structural analysis, which are used for detecting pavement structure failure, estimating expected life, and calculating overlay requirements over a desired design life. Iowa's current FWD networklevel testing protocol requires that pavements are tested at three-drop level with 8-deflection basin collected at each drop level. The test point is determined by the length of the tested pavement section. However, the current FWD network-level program could cover about 20% of Iowa's highway and road systems annually. Therefore, the current FWD network-level test protocol should be simplified to test more than 20% of Iowa's highway and road systems for the network-level test annually. The main objective of this research is to investigate if the minimum number of drop levels and test points could be reduced to increase the testing production rate and reduce the cost of testing and traffic control without sacrificing the quality of the FWD data. Based upon the limited FWD network-level test data of eighty-three composite pavement sections, there was no significant difference between the mean values of three different response parameters when the number of drop levels and test points were reduced from the current FWD network-level testing protocol. As a result, the production rate of FWD tests would increase and the cost of testing and traffic control would be decreased without sacrificing the quality of the FWD data.

Isolation and Characterization of ACC Synthase Gene Family in Mung Bean (Vigna radiata L.): Differential Expression of the Three ACC Synthase enes in Response to Auxin and Brassinosteroid

  • Sunjoo Joo;Kim, Woo-Taek
    • Journal of Plant Biotechnology
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    • v.2 no.2
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    • pp.61-71
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    • 2000
  • By screening a cDNA library of auxin-treated mung bean (Vigna radiata L.) hypocotyls, we have isolated two full-length cDNA clones, pVR-ACS6 and pVR-ACS7, for 1-aminocyclopropane-1-carboxylate (ACC) synthase, the rate-limiting enzyme in the ethylene biosynthetic pathway. While PVR-ACS6 corresponds to the previously identified PCR fragment pMBA1, pVR-ACS7 is a new cDNA clone. A comparison of deduced amino acid sequences among auxin-induced ACC synthases reveal that these enzymes share a high degree of homology (65-75%) to VR-ACS6 and VR-ACS7 polypeptides, but only about 50% to VR-ACS1 polypeptide. ACS6 and ACS7 are specifically induced by auxin, while ACS1 is induced by cycloheximide, and to lesser extent by excision and auxin treatment. Results from nuclear run-on transcription assay and RNA gel blot studies revealed that all three genes were transcriptionally active displaying unique patterns of induction by IAA and various hormones in etiolated hypocotyls. Particularly, 24-epibrassinolide (BR), an active brassinosteroid, specifically enhanced the expression of VR-ACS7 by distinct temporal induction mechanism compared to that of IAA. In addition, BR synergistically increased the IAA-induced VR-ACS6 and VR-ACS7 transcript levels, while it effectively abolished both the IAA- and kinetin-induced accumulation of VR-ACS1 mRNA. In light-grown plants, VR-ACS1 was induced by IAA in roots, whereas W-ACS6 in epicotyls. IAA- and BR-treatments were not able to increase the VR-ACS7 transcript in the light-grown tissues. These results indicate that the expression of ACC synthase multigene family is regulated by complex hormonal and developmental networks in a gene- and tissue-specific manner in mung bean plants. The VR-ACS7 gene was isolated, and chimeric fusion between the 2.4 kb 5'-upstream region and the $\beta$-glucuronidase (GUS) reporter gene was constructed and introduced into Nicotiana tobacum. Analysis of transgenic tobacco plants revealed the VR-ACS7 promoter-driven GUS activity at a highly localized region of the hypocotyl-root junction of control seedlings, while a marked induction of GUS activity was detected only in the hypocotyl region of the IAA-treated transgenic seedlings where rapid cell elongation occurs. Although there was a modest synergistic effect of BR on the IAA-induced GUS activity, BR alone failed to increase the GUS activity, suggesting that induction of VR-ACS7 occurs via separate signaling pathways in response to IAA and BR.

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Compression of Multispectral Images (멀티 스펙트럴 영상들의 압축)

  • Enrico Piazza
    • Journal of Korea Multimedia Society
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    • v.6 no.1
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    • pp.28-39
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    • 2003
  • This paper is an overview of research contributions by the authors to the use of compression techniques to handle high resolution, multi-spectral images. Originally developed in the remote sensing context, the same techniques are here applied to food and medical images. The objective is to point out the potential of this kind of processing in different contexts such as remote sensing, food monitoring, and medical imaging and to stimulate new research exploitations. Compression is based on the simple assumption that it is possible to find out a relationship between pixels close one each other in multi-spectral images it translates to the possibility to say that there is a certain degree of correlation within pixels belonging to the same band in a close neighbourhood. Once found a correlation based on certain coefficient on one band, the coefficients of this relationship are, in turn, quite probably, similar to the ones calculated in one of the other bands. Based upon this second observation, an algorithm was developed, able to reduce the number of bit/pixel from 16 to 4 in satellite remote sensed multi-spectral images. A comparison is carried out between different methods about their speed and compression ratio. As reference it was taken the behaviour of three common algorithms, LZW (Lempel-Ziv-Welch), Huffman and RLE (Run Length Encoding), as they are used in common graphic format such as GIF, JPEG and PCX. The Presented methods have similar results in both speed and compression ratio to the commonly used programs and are to be preferred when the decompression must be carried out on line, inside a main program or when there is the need of a custom made compression algorithm.

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Effect of Different Temperatures on Growing and Flowering of Orostachys japonicus A. Berger (동절기 온도(溫度)에 따른 바위솔의 생장과 개화)

  • Kang, Jin-Ho;Jeon, Seung-Ho;Yoon, Soo-Young;Hong, Dong-Oh;Shin, Sung-Cheol
    • Korean Journal of Medicinal Crop Science
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    • v.13 no.4
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    • pp.186-189
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    • 2005
  • Plant flowering should be mainly affected by temperature and daylength. The study was carried out to measure the effect of temperature during winter on growth and floret flowering of Orostachys japonicus grown under 2 hour night break. Temperature was controlled above $10^{\circ}C$ and $20^{\circ}C$ as heating boilers were run below them to compare the natural temperature. Night-break treatment was done on August 25 and afterward samples were taken every 4 weeks to check growth, morphological and flowering related characters. Plant height, inflorescence length, number of leaves including bracts and stem diameter were increased in heating temperatures of > $10^{\circ}C$ compared to natural temperature although similar in the two heating ones. Leaves and bracts, florets, stem, root, shoot and total dry weights showed the same response as the above morphological and flowering related characters. Florets were more formed but less flowered with higher temperatures. Anthesis rates of individual plant were 100% in natural temperature, 25% in > $10^{\circ}C$ heating temperature but none in > $20^{\circ}C$ heating temperature.