• Korean Journal of Agricultural Science
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• v.44 no.4
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• pp.541-548
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• 2017

This study was conducted to compare in vitro rumen fermentation characteristics among corn grains imported from America, Brazil, Argentina and Ukraine A and Ukraine B. Two Holstein steers, each surgically fitted with a ruminal cannula, consuming total mixed ration were used as rumen fluid donors. In vitro rumen fermentation experiments were performed in a completely random design which included a control (no corn) and treatments with 3.0 g of corn from different geographical origins, i.e., America, Brazil, Argentina, and Ukraine A and Ukraine B, respectively. Ruminal pH, ammonia-N, volatile fatty acid (VFA) and total gas production were measured at 0, 1, 3, 6, 12, 24 and 48 h post-incubation, respectively. No differences (p > 0.05) in mean ruminal pH appeared among the treated groups, however, ruminal pH patterns differed; i.e. corn treated groups had dramatically lower pH compared with control during the entire incubation period. Similarly, no different patterns between the groups in ammonia-N (p > 0.05) appeared until 6 h post-incubation. Unexpectedly, higher ammonia-N concentration for control than that for the corn treated groups appeared after 12 h post-incubation despite that for all groups increased. Total VFA was similar between the groups until 6 h post-incubation, but VFA after 12 h post-incubation was different (p < 0.05), i.e. VFA for corn from Argentina, Ukraine A, Ukraine B, and Brazil were comparatively higher than for America. Overall, data in this study showed that the corns of different origins may have different feed values to ruminants despite having similar chemical compositions.

• Korean Journal of Veterinary Research
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• v.22 no.2
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• pp.111-114
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• 1982

In order to explore more efficient protein substitutes by improving the utilization of non-protein nitrogen compounds in ruminants, the experiment was undertaken. The effects of sulfur coated urea (SCU) and diuredio isobutane (DUIB) in the ruminal fluid on the concentration of $NH_3-N$, the total count of the ruminal ciliates and pH value were estimated in vitro. The results obtained from the experiment were as follows: 1. The pH of the media of the group added diuredio isobutane and sulfur costed urea tends to decrease slightly at 9 hours after the incubation, but no pH changes were observed in the media added urea alone. 2. The number of the ciliates in the ruminal fluid was slightly increased 9 hours after the incubation in all groups. 3. The concentrations of $NH_3-N$ in the ruminal fluid were gradually increased according to the incubation in vitro showing 418, 431 and $627{\mu}g/ml$ in the group added diuredio isobutane and 428, 569 and $792{\mu}g/ml$ in the group added sulfur coated urea at 0, 0.5 and 9 hours after the incubation, respectively.

• Asian-Australasian Journal of Animal Sciences
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• v.27 no.12
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• pp.1726-1735
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• 2014

This study investigated the effects of acarbose addition on changes in ruminal fermentation characteristics and the composition of the ruminal bacterial community in vitro using batch cultures. Rumen fluid was collected from the rumens of three cannulated Holstein cattle fed forage ad libitum that was supplemented with 6 kg of concentrate. The batch cultures consisted of 8 mL of strained rumen fluid in 40 mL of an anaerobic buffer containing 0.49 g of corn grain, 0.21 g of soybean meal, 0.15 g of alfalfa and 0.15g of Leymus chinensis. Acarbose was added to incubation bottles to achieve final concentrations of 0.1, 0.2, and 0.4 mg/mL. After incubation for 24 h, the addition of acarbose linearly decreased (p<0.05) the total gas production and the concentrations of acetate, propionate, butyrate, total volatile fatty acids, lactate and lipopolysaccharide (LPS). It also linearly increased (p<0.05) the ratio of acetate to propionate, the concentrations of isovalerate, valerate and ammonia-nitrogen and the pH value compared with the control. Pyrosequencing of the 16S rRNA gene showed that the addition of acarbose decreased (p<0.05) the proportion of Firmicutes and Proteobacteria and increased (p<0.05) the percentage of Bacteroidetes, Fibrobacteres, and Synergistetes compared with the control. A principal coordinates analysis plot based on unweighted UniFrac values and molecular variance analysis revealed that the structure of the ruminal bacterial communities in the control was different to that of the ruminal microbiota in the acarbose group. In conclusion, acarbose addition can affect the composition of the ruminal microbial community and may be potentially useful for preventing the occurrence of ruminal acidosis and the accumulation of LPS in the rumen.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.4
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• pp.501-506
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• 2001

This experiment was conducted to evaluate the effects of supplemental unsaturated fatty acids (UFA) on fermentation characteristics, especially on gas production, cellulose degradation and volatile fatty acid (VFA) concentration by mixed ruminal microorganisms. In order to attain this objective, unsaturated fatty acids including oleic acid (C 18:1), linoleic acid (C18:2) and arachidonic acid (C22:4) were added at varying level. Mixed ruminal microbes used in this experiment were obtained from the rumen of a cannulated Holstein cow. Medium pH values after 7 d incubation were significantly affected by type and level of unsaturated fatty acids (p<0.01). All of UFA inhibited total gas production, and especially treatment of arachidonic acid at the levels of 0.01% gave the lowest gas. production after 7 d incubation (p<0.01). Comparison of the population of protozoa revealed that UFA did not have any significant effect on the total protozoa number. The addition of UFA did not effect dry matter degradation. Volatile fatty acid (VFA) composition of the culture was influenced little by UFA, although the considerable amount of iso-type VFA were detected in UFA supplemented incubations. The ratio of acetic acids to propionic acids, however, was lower than control in all the treatments after 7 d incubation (p<0.01).

• Asian-Australasian Journal of Animal Sciences
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• v.26 no.4
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• pp.523-528
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• 2013

This study investigates the effects of three branched-chain amino acids (BCAA; valine, leucine, and isoleucine) on the in vitro ruminal fermentation of wheat straw using batch cultures of mixed ruminal microorganisms. BCAA were added to the buffered ruminal fluid at a concentration of 0, 2, 4, 7, or 10 mmol/L. After 72 h of anaerobic incubation, pH, volatile fatty acids (VFA), and ammonia nitrogen ($NH_3$-N) in the ruminal fluid were determined. Dry matter (DM) and neutral detergent fiber (NDF) degradability were calculated after determining the DM and NDF in the original material and in the residue after incubation. The addition of valine, leucine, or isoleucine increased the total VFA yields ($p{\leq}0.001$). However, the total VFA yields did not increase with the increase of BCAA supplement level. Total branched-chain VFA yields linearly increased as the supplemental amount of BCAA increased (p<0.001). The molar proportions of acetate and propionate decreased, whereas that of butyrate increased with the addition of valine and isoleucine (p<0.05). Moreover, the proportions of propionate and butyrate decreased (p<0.01) with the addition of leucine. Meanwhile, the molar proportions of isobutyrate were increased and linearly decreased (p<0.001) by valine and leucine, respectively. The addition of leucine or isoleucine resulted in a linear (p<0.001) increase in the molar proportions of isovalerate. The degradability of NDF achieved the maximum when valine or isoleucine was added at 2 mmol/L. The results suggest that low concentrations of BCAA (2 mmol/L) allow more efficient regulation of ruminal fermentation in vitro, as indicated by higher VFA yield and NDF degradability. Therefore, the optimum initial dose of BCAA for in vitro ruminal fermentation is 2 mmol/L.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.7
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• pp.979-982
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• 2003

Two rapeseed meal samples (Sample A, hybrid 5900 and sample B, double low rapeseed No.4) obtained from China and one Canola meal sample obtained from a local crushing plant in Canada were used to investigate the amino acid degradability of rapeseed/Canola meal in rumen and amino acid digestibility of ruminal incubation residues by precision-fed rooster bioassay. Results show that in ruminal incubation the degradation rate of non amino acid nitrogen in crude protein is higher than that for amino acid nitrogen in crude protein, the results also suggest that the degradation rate of amino acid nitrogen in Chinese rapeseed meal sample B was lower than that for Canadian Canola, but that in Chinese rapeseed meal sample A is much close to that for Canadian canola meal. For all amino acids the digestibility of the bypass or residual protein as measured by the precision-fed rooster bioassay tended to be lower for Chinese rapeseed meal sample A than for sample B or Canadian canola meal which had similar digestibility values. However following a calculation of total amino acid availability, involving the digestibility of amino acids in the rumen and rooster bioassay the results are less contradictory. Results indicated that in traditional roasting-expelling process, heat treatment, especially dry heat treatmeat could decrease amino acids degradability in rumen of rapeseed/canola meal, but also may decrease total availability of amino acids of rapeseed/canola meal.

• Journal of The Korean Society of Grassland and Forage Science
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• v.28 no.3
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• pp.255-264
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• 2008

This study was conducted to evaluate the effects of rumen protected choline on in vitro ruminal fermentation and milk production and its composition in Holstein cows. Experiments were done with three treatment groups, basal diet without any supplement (T1), basal diet+23g/d of mixture of choline and wheat shorts (T2) and basal diet + 25.56 g/d of rumen protected choline (T3). The in vitro ruminal pH and ammonia concentrations were similar for three treatments during all incubation periods except for the in vitro ruminal pH on 3 hr incubation and ammonia concentrations on 9 hr incubation. No significant difference was found in the concentrations of acetate and total-VFA. The propionate and butyrate concentrations were not affected by the rumen protected choline except on 6 hr incubation on which the propionate and butyrate concentrations were intermediate (8.98 mg/dl) and least (3.22 mg/dl), respectively. Higher milk yield and milk fat and lactose were resulted in the rumen protected choline. However, the rumen protected choline did not affect the milk protein, solids not fat, total solids, MUN, somatic cell count. It is concluded that the rumen protected choline can be effective materials to improve the milk production, milk fat and lactose without little change on in vitro ruminal fermentation.

• Korean Journal of Agricultural Science
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• v.45 no.3
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• pp.419-427
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• 2018

The present study was conducted to investigate the effect of different levels of Korean corn grain on in vitro ruminal fermentation with total mixed ration (TMR) as a basal feed. Three ruminal cannulated Holstein steers (Body Weight $479{\pm}33.0kg$) were used as rumen fluid donors. Treatments for in vitro fermentation were TMR only (control, 3.0 g), TMR substituted partially with high level (HC, TMR 1.5 and corn 1.5 g), and with low level of Korean corn grain (LC, TMR 2.25 and corn 0.75 g), respectively. To measure in vitro ruminal pH, gas production, ammonia N and volatile fatty acids (VFA), the in vitro fermentation incubation was triplicated at $39^{\circ}C$, 120 rpm for 0, 1, 3, 6, 12, 24 and 48 h, respectively. Mean ruminal pH was significantly lower (p < 0.05) for HC than control. Changes in rumen pH was rather similar between the groups till 6 h after incubation, but the lowest pH for HC (pH 5.10) appeared at 48 h compared with control and LC. Total gas production was tended (p < 0.09) to be higher and ammonia N was significantly lower (p < 0.05) for HC than control and LC. Total VFA was higher (p < 0.05) for HC and LC than control but no differences appeared between HC and LC. Overall, the present data indicate that feeding different levels of Korean domestic corn grain may lead to high and sustainable starch degradation in the rumen.

• Asian-Australasian Journal of Animal Sciences
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• v.9 no.1
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• pp.75-81
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• 1996

The dry matter (DM) disappearance and ruminal solubility of calcium (Ca), phosphorus (P), magnesium (Mg) and potassium (K) in eight Philippine forages were studied. The forages were: paragrass (Brachiaria mutica (Forsk.) Stapf), stargrass (Cynodon plectostachyum Pilger), napiergrass (Pennisetum purpureum Schumach), clopo (Calopogonium mucconoides Desv.), centrocema (Centrocema pubescens Benth.), gliricidia (Gliricidia sepium (Jacq.) Walp.), leucaena (Leucaena leucocephala (Lam.) de Wit.) and sesbania (Sesbania grandiflora (L.) Poir. Nylon bags with samples were incubated for 0, 3, 6, 12, 24, 48 and 72 h in rumen cannulated sheep. The 0-h bags were washed with deionized water. For the 0-h samples, 20.4, 17.2, 50.7, 52.2 and 80.1% of the DM, Ca, P, Mg and K was solubilized, respectively. At 3-h incubation period, DM disappearance was 10 percentage units higher than that of 0-h incubation whereas mineral disappearance increased by 43, 21, 30 and 13% for Ca, P, Mg and K, respectively. At 72-h incubation period, greater proportion of DM, Ca, especially in P, Mg and K was solubilized with a value of 73.8, 71.5, 85.6, 91.4 and 98.2%, respectively. The average particulate passage rate obtained in the present study was 1.9%/h where as the range of disappearance rates of various mineral elements were : 0.4 to 1.2%/h for Ca, 0.1 to 1.6%/h for P, 0.7 to 2%/h for Mg and 0.1 to 2%/h for K. The effective ruminal solubilization (ERS) of the macrominerals was calculated where particulate passage rate and disappearance rate of the various elements were included in the equation. The ERS of Ca, P, Mg and K was 50.0, 72.6, 83.9 and 94.5%, respectively. Species differences (p<0.05) on the various mineral solubilities were also observed. This study shows that ruminal solubility of macrominerals in selected Philippine forages is K > Mg > P > Ca.

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.2
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• pp.176-181
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• 2000

In order to clarify the inhibitory effects of orchardgrass (Dactylis glomerata L.) lipids on ruminal fermentation and digestion, two experiments were carried out in vitro. Experiment 1 was carried out using residues of grass hay from which the lipid fraction was removed by ether extraction. To ground grass samples were added 0, 1.5, 3.0, 4.5 and 6.0% lipids and incubated anaerobically at $39^{\circ}C$ for 24 h, with the mixtures of artificial saliva and rumen fluid. Increasing grass lipid levels remarkably reduced DM and NDF disappearances. Volatile fatty acid concentration was significantly reduced at 3.0, 4.5 and 6.0% lipid levels. Microbial nitrogen proportion to total nitrogen tended to decrease by the addition of the lipids. These results indicated that grass lipids have a marked inhibitory effect on ruminal fermentation and digestion, especially when to the substrate was added 3% or more grass lipids as ether extracts. Experiment 2 was conducted to study the relationship between changes in the free fatty acids and changes in the fermentation traits. Samples were incubated for 3, 6, 9, 12, 15, 18, 21 and 24 h as a sole substrate. The polyunsaturated fatty acids steadily decreased during incubation, whereas the saturated fatty acid ($C_{18:0}$) increased. It was suggested that the hydrogenation was extended during the initial stage of incubation. The unsaturated fatty acids ($C_{18:2}$, $C_{18:3}$) produced at the initial stage of incubation were negatively correlated with the amount of microbial N and DM disappearance, indicating that polyunsaturated fatty acids had the possibility to show an inhibiting effect on ruminal fermentation and digestion.