• Korean Journal of Plant Resources
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• v.18 no.2
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• pp.260-269
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• 2005

Taxonomic studies on morphological, principal component analysis (PCA), palynological, RAPD and PCR-RELP analysis were conducted to intraspecific relationships of Rubus oldhami. Three types of Rubus oldhami based on the flower characters such as petal length and number were used in this study. Among the 14 morphological characters, perianth length, calyx lobe length, apical leaflet shape and leaflet length were used to distinguish for each type. The pollen characters such as shape, aperture number, surface sculpture were showed very similar among three types. Eight primers out of 20 arbitrary primers were screened for three types, and were revealed 33 ($60\%$) polymorphic bands. The phonogram by RAPD data showed incongruent with morphological analysis. Even though ten restriction endonucleases produced 20 restriction sites, polymorphic bands were not observed. Based on the results, three types of Rubus oldhami divided well by morphological characters, but pollen and DNA data were not supported. Therefore, type 1 and 2 which different from type 3 by flower characters considered as a temporary hybrid or ecotype because of their similar habitats.

• Korean Journal of Pharmacognosy
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• v.39 no.2
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• pp.123-126
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• 2008

The five flavonoids, isoqueritrin, juglanin, astragalin and 2-O-trans-p-coumaroyl astragalin were quantitatively analyzed in the leaves, unripe- and ripe fruits of four Korean Rubus species including Rubus crataegifolius, R. pungens var. oldhami, R. parvifolius, and R. coreanus belonging to the Rosaceae family. The HPLC analysis demonstrated that flavonoids are more abundant in the leaves than in the fruits. In the four Rubus species, ripe fruits generally contained similar or more amount of flavonoids than in unripe fruits. In particular, the quantity levels of total flavonoid (49.81${\pm}$0.50 mg/g) and isoquercitrin (35.08${\pm}$0.37 mg/g) were highest in the leaf of R. crataegifolius. In addition, the content of the flavonoids were quite low in the unripe fruits of R. coreanus that is most widely used as Rubi Fructus.

• Korean Journal of Pharmacognosy
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• v.38 no.2 s.149
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• pp.187-191
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• 2007

The extraction yield of the methanolic extracts and 19${\alpha}$-hydroxyursane-type triterpenoid (19${\alpha}$-HUT) fraction were investigated in the unripe and ripe fruits and the leaves of the four Rubus plants (Rubus coreanus, R. crataegifolius, R. phoenicolasius, R. pungens var. oldhami) to develop the biomaterial 19${\alpha}$-HUT mixture as functional foods. Thin layer chromatogaphy-Densitometer (TLC-DM) was used to analyze the individual quantity of 19${\alpha}$-HUTs using standard compounds (euscaphic acid, tormentic acid, 23-hydroxytormentic acid, kaji-ichigoside F$_1$, rosamultin, niga-ichigoside F$_1$). The content of methanolic extract of the fruits were higher in the ripe stage than in the unripe stage whereas the content of 19${\alpha}$-HUT mixture varied with each Rubus species. The Rubus plants containing the highest amount of 19${\alpha}$-HUTs in the leaves were R. coreanus, R. phoenicolasius and R. pungens var. oldhami while only R. cratagefolius showed the highest content in the ripe fruits. The mean of total genin content of 19${\alpha}$-HUTs was 0.94 mg/g; that of the glycosides was 0.60 mg/g. The genin quantity was found in the order of 23-hydroxytormentic acid> euscaphic acid> tormentic acid; the glycoside was observed in the order of niga-ichig-oside F$_1$> kaji-ichigoside F$_1$> rosmaultin, by which the biosynthetic pathway of 23-hydroxytormentic acid and its glucoside niga-ichigoside F$_1$ via the intermediates tormentic acid and/or rosamultin was presumed. It is also suggested that the ripe fruits of R. crataegifolius will be desirable to use as functional foods rather than unripe fruits.

• Natural Product Sciences
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• v.20 no.4
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• pp.290-295
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• 2014

The triterpenoids contained in four Rubus species (Rosaceae) were quantitatively analyzed using HPLC to select plants with large quantities of niga-ichigoside $F_1$. Unripe fruits, ripe fruits, and leaves were extracted to estimate the quantity of niga-ichigoside $F_1$, together with Rubus-specific $19{\alpha}$-hydroxyursane-type triterpenoids, euscaphic acid, tormentic acid, and kaji-ichigoside $F_1$, and a dimeric triterpenoid coreanoside $F_1$. Niga-ichigoside $F_1$ was most abundant in the leaves of R. crataegifolius (23.4 mg/g dry weight). The amount of triterpenoid in the black, ripe fruits of R. coreanus was lower than the unripe fruits of the same plant. On the other hand, the ripe fruits of three plants, R. crataegifolius, R. parvifolius and R. pungens var. oldhami, which are reddish, contained higher or similar level of triterpenoids than their unripe fruits. In addition, the concentration of niga-ichigoside $F_1$ in the ripe fruit of R. crataegifolius was 20.5 mg/g, suggesting that the fruits could be used as a functional food. Methyl gallate and ellagic acid were used as quantitative indices of total tannin. Methyl gallate levels were higher in ripe fruits than unripe fruits in R. crataegifolius, R. pungens var. oldhami, and R. parvifolius. In R. crataegifolius, the quantity of methyl gallate was 30.5 mg/g in ripe fruit, but 1.19 mg/g in unripe fruit.

• Natural Product Sciences
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• v.20 no.4
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• pp.258-261
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• 2014

Antidepressant miquelianin (quercetin 3-O-glucuronide) was isolated from the leaves of Rubus craetaegifolius (Rosaceae) and identified by physical and spectroscopic data. Miquelianin was quantitatively analyzed in the leaves, mature and premature fruits of Korean wild R. craetagifolius, R. pungens var. oldhami, R. coreanus, and R. parvifolius by HPLC. The contents of miquelianin was highest in the mature fruit of R. crataegifolius ($16.29{\pm}0.79mg/g$); however, the content of kaempferol 3-O-glucuronide was $33.88{\pm}7.68mg/g$. These results suggest that the mature fruit of R. crataegifolius would be beneficial for treating depression or stress as a functional food with its sweet taste.

• The Korean Journal of Ecology
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• v.19 no.6
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• pp.575-582
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• 1996

Molipteryx fuliginosa (Uhler, 1860) is a plant juice sucker which feeds on new tips of Rubus oldhami Miquel and Zelkowa serrata Makino, and it has a strong preference for teses two plants in Korea. M. fuliginosa has one generation a year and hibernates as a young adult. Most of the winter survivors emerge in early May. It is the first time their host plants were found and reported. Females mainly lay their eggs one by one separately on the leaves of R. oldhami, and even on the steel wire, the lid guaze and the ground in the laboratory. Nymphs do not gather, but stay on the hatching site, Nymphs except the non-feeding first instar feed on young shoots. From the second to the fifth instar nymphs migrate to the upper part of the shoot and congregate in part on an expanded leaf. The new adults first appeared on 11 August, and remained in the host plant, and fed on until mid October. The duration of the hatching and molting, and the survivorship curve based on the laboratory rearing were determined.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.14 no.4
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• pp.25-40
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• 2011

The vascular plants in Jeokjabong, Bogil Island were listed 350 taxa composed of 89 families, 233 genera, 309 species, 1 subspecies, 36 varieties and 4 forms. Divided into woody plants were 142 taxa (40.6%) and herbaceous plants were 208 taxa (59.4%). Based on the list of rare and endangered species by Korea Forest Service and Korea National Arboretum, 3 taxa were recorded; Celtis choseniana, Asarum maculatum, Rubus sorbifolius, and endemic plants, 10 taxa were recorded; Asarum maculatum, Cephalotaxus koreana, Carpinus coreana, Celtis choseniana, Lespedeza maritima, Indigofera koreana, Dendropanax morbifera, Ligustrum quihoui var. latifolium, Weigela subsessilis, Carex okamotoi, and plant species subject to permission for taking abroad, 4 taxa were recorded; Celtis choseniana, Asarum maculatum, Vaccinium oldhami, Carex okamotoi. Based on the list of floristic regional indicator plants by Korean Ministry of Environment were total 64 taxa (18.3% of all 350 taxa of vascular plants); Ligustrum quihoui var. latifolium in class IV, 19 taxa (Stauntonia hexaphylla, Cinnamomum japonicum, Zanthoxylum ailanthoides, Daphniphyllum macropodum, Centella asiatica, Verbena officinalis, Mitchella undulata, etc.) in class III, 5 taxa (Potentilla dickinsii, Ostericum melanotilingia, Sanicula rubriflora, Caryopteris incana, Teucrium veronicoides) in class II and 39 taxa (Gleichenia dichotoma, Cyrtomium fortunei, Aphananthe aspera, Rubus hirsutus, Meliosma myriantha, Hedera rhombea, Ainsliaea apiculata, etc.) in class I. Based on the list of naturalized plants, 5 families, 17 genera, 20 taxa (Phytolacca americana, Lepidium virginicum, Robinia pseudo-acacia, Verbesina alternifolia, Lolium perenne, etc.) and naturalization rate was 5.7% of all 350 taxa of vascular plants. Hereafter natural ecosystem into disturbance on naturalized plants and legal protection species and rare and endangered species distribute in the Bogil Island, precision investigation after management counterplan of the monitoring a point of view be required.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.11 no.2
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• pp.66-74
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• 2008

In recent years, the view centered conservation for eco-system is getting important rather than development from human oriented perspective. As a result, the concept "Sustainable development", that is seeking harmony between conservation and development, appeared so that we researched distribution and status of vascular plants a site of project in the Bukkyungnam substation. The main findings from the study is as follows. The vascular plants a site of project in the Bukkyungnam substation area was listed 435 taxa; 93 families, 269 genera, 377 species, 1 subspecies, 51 varieties and 6 forms. Specific plant species by floral region were total 25 taxa; Acer palmatum (Planted species), Asperula lasiantha in Class III, Caryopteris incana, Cirsium schantarense in Class II, 21 taxa (Dryopteris erythrosora, Chloranthus japonicus, Salix glandulosa, Alnus hirsuta, Quercus variabilis, Ulmus parvifolia, Clematis patens, Rubus hirsutus, Euphorbia pekinensis, Cayratia japonica, Grewia biloba var. parviflora, Vaccinium oldhami, Microstegium japonicum, Hosta capitata, Polygonatum falcatum, etc.) in Class I. Based on the list of Korean endemic plants, 10 taxa were recorded; Cephalotaxus koreana, Populus tomentiglandulosa (Planted species), Indigofera koreana, Forsythia koreana (Planted species), Paulownia coreana (Planted species), Asperula lasiantha, Weigela subsessilis, Adenophora triphylla var. hirsuta, Carex okamotoi, Lilium amabile and based on the list of the rare plants by the Forest ResearchInstitute, 2 taxa were recorded in the studied areas; Lilium callosum (Preservation priority order; No. 191), Aristolochia contorta (No. 151). The naturalized plants in this site were 13 families, 22 genera, 28 taxa and naturalization rate was 6.4% of all 435 taxa vascular plants.