• Asian Pacific Journal of Cancer Prevention
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• v.17 no.4
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• pp.1973-1978
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• 2016

Background: Breast cancer is a heterogeneous disease that represents a major public health problem. The immunohistochemical determination of breast cancer subtypes with regard to estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor (HER2) status can contribute to improved selection of therapy and patientcare. The purpose of this study was to determine the prevalence of the molecular breast cancer subtypes and to assess their associations with classical clinicopathologic parameters for better therapeutic decisions in women with breast cancer in the Ivory Coast. Materials and Methods: Formalin-fixed and paraffin-embedded blocks of patients diagnosed with primary breast carcinoma were subjected to immunohistochemical assay for the assessment of ER/RP and HER2 expression. The one-way analysis of variance evaluated the difference between breast cancer subtypes and mean age of patients. The Chi-square Test was used to compare standard clinicopathologic prognostic parameters with tumor subtypes. Results. Among 302 patients, 57% were premenopausal and 43% were postmenopausal. The invasive ductal carcinoma not otherwise specified (IDC NOS) (82.8%) was the most frequent histological type, and the tumor grade 2 (56%) was predominant followed by grade 3 (20.9%). The proportion of positivity of ER, PR, and HER2 was 56%, 49%, and 15.6%, respectively. Half of patients of this study (51.6%) had luminal A breast tumor type followed by TN (32.1%). Other subtypes were luminal B (10.1% ) and non-luminal HER2+ (6.3%). Conclusions. The findings of the present study are in line with the literature and should assist in management of breast cancer in our country.

• Korean Journal of Animal Reproduction
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• v.18 no.3
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• pp.191-197
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• 1994

It is generally known that mutations in any of several genes encoding photoreceptor-specific proteins have resulted in retinitis pigmentosa (RP), a disease characterized by losing photoreceptor function with progressive degeneration of photoreceptor cells and eventually leading to blindness. To study the procure and cure of photoreceptor degeneration, we produced transgenic mice. Transgene consisted of a 12.5kb genomic DNA fragment that contains mutated pig rhodopsin gene (Pro-347-Ser) including both the 5'-franking (4.0 kb) and the 3'-franking (2.9 kb) sequences. This gene was used for the production of transgenic mouse. The mutated rhodopsin DNA was microinjected into male pronuclei of fertilized mouse (C57BL /6]) embryos. We detected transgenic animals harboring mutated rhodopsin gene by PCR and Southern blot analysis. These transgenic mice showed stable transmission of microinjected rhodopsin gene into their offspring. Therefore these animals will provide a novel approach to study the mechanism of the photoreceptor degeneration and be provided as a disease model for the treatment of the blind in human.

• Plant Journal
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• v.15 no.1
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• pp.38-44
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• 2019

This research is an experimental investigation to find the desirable pH of slurry in the desulfurization absorber for a 1000 ㎿ coal fired power plant, operating in compliance with the Air Environmental Conservation Act and the plant's internal regulations. In case the average sulfur dioxide concentration in inflow flue gas, ${\bar{C\;in}}$ [ppm] changed to 500 ppm, 550 ppm, 600 ppm and 635 ppm after fixing inflow flue gas flow rate, generator output, pressure drop in the absorber, and oxidation air flow rate, the desirable pH of the slurry in the absorber, was 5.0, 5.2, 5.3 and 5.4. Thus, it is recommended that the desirable pH of slurry is calculated using the correlation equation, $RpH=0.0018{\times}{\bar{C\;in}}+4,2031$ when the average sulfur dioxide concentration in the inflow flue gas is in the range of 500 ppm to 635 ppm.

• The Journal of Korean Academy of Prosthodontics
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• v.47 no.4
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• pp.424-434
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• 2009

Statement of problem: Problems such as loosening and fractures of retained screws and fracture of implant fixture have been frequently reported in implant prosthesis. Purpose: Implant has weak mechanical properties against lateral loading compared to vertical occlusal loading, and therefore, stress analysis of implant fixture depending on its material and geometric features is needed. Material and methods: Total 28 of external hexed implants were divided into 7 of 4 groups; Group A (3i, FULL $OSSEOTITE^{(R)}$Implant), Group B (Nobelbiocare, $Br{\aa}nemark$ $System^{(R)}$Mk III Groovy RP), Group C (Neobiotec, $SinusQuick^{TM}$ EB), Group D (Osstem, US-II). The type III gold alloy prostheses were fabricated using adequate UCLA gold abutments. Fixture, abutment screw, and abutment were connected and cross-sectioned vertically. Hardness test was conducted using MXT-$\alpha$. For fatigue fracture test, with MTS 810, the specimens were loaded to the extent of 60-600 N until fracture occurred. The fracture pattern of abutment screw and fixture was observed under scanning electron microscope. A comparative study of stress distribution and fracture area of abutment screw and fixture was carried out through finite element analysis Results: 1. In Vicker's hardness test of abutment screw, the highest value was measured in group A and lowest value was measured in group D. 2. In all implant groups, implant fixture fractures occurred mainly at the 3-4th fixture thread valley where tensile stress was concentrated. When the fatigue life was compared, significant difference was found between the group A, B, C and D (P<.05). 3. The fracture patterns of group B and group D showed complex failure type, a fracture behavior including transverse and longitudinal failure patterns in both fixture and abutment screw. In Group A and C, however, the transverse failure of fixture was only observed. 4. The finite element analysis infers that a fatigue crack started at the fixture surface. Conclusion: The maximum tensile stress was found in the implant fixture at the level of cortical bone. The fatigue fracture occurred when the dead space of implant fixture coincides with jig surface where the maximum tensile stress was generated. To increase implant durability, prevention of surrounding bone resorption is important. However, if the bone resorption progresses to the level of dead space, the frequency of implant fracture would increase. Thus, proper management is needed.

• Journal of Life Science
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• v.24 no.3
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• pp.234-241
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• 2014

To identify and isolate anticancer active compounds from Solanum nigrum, S. nigrum was extracted with MeOH and then fractionated with various organic solvents ($CH_2Cl_2$, EtOAc, n-BuOH, and $H_2O$). The cytotoxic effects of the MeOH extracts from S. nigrum and its organic solvent-soluble fractions were also tested in HT29 cells. All the MeOH extracts of S. nigrum and its organic-solvent extracts induced cytotoxicity in the HT29 cells. Among the extracts, $H_2O$ was the most effective. The $H_2O$ extract was purified further by repeated silica gel, Sephadex LH-20, Diaion HP- 20, and RP-18 column chromatography. An active anticancer compound, Des-N-26-methylene-dihydrotomatidine, was isolated with a molecular weight of 416 and a molecular formula of $C_{28}H_{48}O_2$. Analysis of the cytotoxic effects of Des-N-26-methylene-dihydrotomatidine on the HT29 cells compared to those of tomatine and tomatidine are similar in its structure, is higher than tomatidine above the 40 ${\mu}g/ml$ concentration, but lower than tomatine. This is the first study to describe the anticancer activity of Des-N-26-methylene-dihydrotomatidin, isolated from S. nigrum. Des-N-26- methylene-dihydrotomatidine seems to have potential as a natural bioactive compound.

• Journal of Life Science
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• v.19 no.1
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• pp.9-14
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• 2009

The prostate cancer is the critical health problem, increasing of its related death in worldwide. Unfortunately present surgery and chemotherapeutic choices seem to be impossible in curing or controlling prostate cancer, because metastasis occasionally advances even after these potentially curative therapies. Therefore, there is immediate need to alternative chemoprevention and chemotherapeutic agents. Over one hundred species of dried medicinal herbs were tested for proliferation inhibitory effects on prostate cancer cell line, PC-3. One of them, Anthriscus sylvestris was selected because of potent anti-proliferation effect. The dried root of A. sylvestris was extracted with 100% methanol for 2-3 days and its extract was fractionated by using ethyl acetate. And ethyl acetate layer was subjected to column chromatographies on silica gel, reverse phase-18 (RP-18) and Sephadex LH-20, in turn. Finally, the pure compound was obtained by crystallization in methanol at $4^{\circ}C$ for overnight and identified as deoxypodophyllotoxin by NMR spedorscopic and physico-chemical analyses. In addition, it was confirmed that deoxypodophyllotoxin clearly inhibits the proliferation of PC-3 cells in a dose and time-dependent manner.

• Korean Journal of Microbiology
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• v.30 no.2
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• pp.101-107
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• 1992

Cytochrome c oxida5e from chemotrophically grown R p , geliitinosu was purified by cytochrome c affinity chromatography and DEAE-Sephacel ion exchange chromatography. The molecular weight of the cytochrome c oxidase was approximately 110.000 Da by sephacryl s-300 gel chromatography and approximately 52, 000 Da by SDS-gel electrophoresis, respectively. Therefore. cytochrolne c oxidase of Rps. gehtinosu seems to be dimer. The cytochrome c oxidasc was very sensitive to temperature. It's Km and Vmax were 20 pM and 44 unitlmg protein for horsc heart cytochrome c as a substrate. respectively, and its optimum pH and temperature were 6.4 and 25$^{\circ}$C. respectively. The absorption peaks of the reduced cytochrome c oxidase showed at 554 nm, 523 nm. and 422 nm. The activiiy of cytochrome c oxidase was inhibited by KCN, and NaN3, but not by CO, antimycir~ A. and myxothiazol. The cytochrome c-551 was produced either in phototrophically or chemotrophically grown Rps. gelaiinosci. The rcduced cytochrome c-551 was oxidized by b-type cytochrome c oxidase from Rp.v. gc.lrtino.sc~. Km and Vmax of cytochrome c oxidase was 26 pM and 31 unitlnlg protein For cytochrome c-551 as a substrate. respectively. Thercfore. thc electron transfer chain of chemotrophically grown Rps. glatinosa seems lo be ubiquinol cytochrome bc, complex -'cytochrome c-55lMb-type cytochrome c oxidase+02.

• 한국신재생에너지학회:학술대회논문집
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• 2010.11a
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• pp.139.1-139.1
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• 2010

제 3차 국가에너지기본계획이 시행됨에 따라 전체 에너지 공급량 중 신재생에너지가 차지하는 비율은 점차 늘어날 전망이다. 이와 관련하여 2012년부터 RPS 시행이 확정되었다. RPS(신재생에너지 의무비율할당제)는 신재생에너지에 의한 발전 비율을 설정함으로써 공급을 늘리는 긍정적인 측면이 있으나 이와 동시에 전기요금 인상을 수반한다. 요금 인상으로 인하여 발생할 수 있는 소비자의 반감을 완화시키고 전기요금에 대한 현실적인 합의점을 찾으려면 소비자가 재생에너지 전력에 대하여 어느 정도의 지불의사를 가지고 있는지에 대한 연구가 선행적으로 진행되어야 한다. 한편 RPS의 도입은 신재생에너지 원간 가격경쟁을 촉진시키게 된다. 이 과정에서 성장잠재력이 높은 에너지원이라 하더라도 현재의 발전단가가 높다면 잠재력을 발휘하기 전에 도태될 가능성이 있다. 이러한 RPS 도입의 영향에 대처할 수 있는 정책수립의 기초자료를 마련하기 위해, 재생에너지 전력에 대한 소비자 측면의 효용이 원별로 분석될 필요가 있다. 본 연구에서는 조건부 가치평가법(Contingent Valuation)을 적용하여 수력, 풍력, 태양광으로 발전된 재생에너지 전력에 대한 지불의사액(Willingness to Pay)을 추정하였다. 단일 설문지 내에서 여러 에너지원에 대한 지불의사액을 순차적으로 물어보게 될 경우, 질문순서가 지불의사액 추정에 편의를 가져오는 순서효과(Sequence Effect)가 발생할 수 있다(Boyle et al.,1993). 이러한 순서효과를 제거하기 위해서 유형에 따라 질문순서를 다르게 하여 각 에너지원에 대한 지불의사액을 묻는 방향으로 설문을 설계하였다. 분석결과 수력, 풍력, 태양광으로 발전한 전력에 대한 지불의사액 간에는 유의한 차이 가 나타나지 않았으며, 순서효과로 인한 편의 또한 지불의사액에 유의한 영향을 미치지 않는 것으로 관측되었다.

• Journal of The Korean Society of Grassland and Forage Science
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• v.11 no.2
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• pp.90-96
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• 1991

This experiment was carried out in order to estimate the recovery days of root and stubble to the days after cutting, and contribution of specific stubble weight on the regrowth was examined using the relationships between the dry weight of shoot and yield components, and regrowth parameters by the days after cutting. The varieties examined were Maprima, Manhattan, Tove, Peramo, Caliente, Tempo and P-2 grown under individual plant basis. The results are may be summarized as follows: 1. Dry weight of root and stubble were recovered up to 13.5 and 11 days after cutting, respectively. 2. Dry weight of shoot(regrowth parts+stubble) was affected significantly by the varieties, stages of regrowth and variety x stage of regrowth. 3. The variety with tiller weight type showed higher average productivity of shoot than those of the variety with tiller number type. 4. Absolute growth rate(AGR) of shoot was correlated significantly with regrowth parts, stubble, root and weight of a tiller at the early stage of regrowth(up to 12 days after cutting), and correlated with regrowth parts, stubble, weight of tiller and stubble area at the late stage of regrowth(up to 20 days after cutting). 5. Contribution of specific stubble weight to absolute growth rate of shoot was different between the stages of regrowth. Thus, regrowth parts per specific stubble weight(RP1SSbW) and weight of tiller per specific stubble weight(WT1SSbW) contributed to absolute growth rate of shoot at the early stage of regrowth, and efficiency of specific stubble weight(ESSbW), regrowth parts per specific stubble weight (RPISSbW) and weight of a tiller per specific stubble weight(WT1SSbW) contributed to absolute growth rate of shoot at the late stage of regrowth. 6. Regrowth utilization rate(RUR) was one of the useful regrowth parameter to indicate the regrowth potential of grasses.

• Journal of the Korean housing association
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• v.20 no.3
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• pp.113-121
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• 2009

The purpose of this study is to determine color preferences in housing spaces according to different types of personalities and color preference according to classifying dwellers various characteristics by MBTI to obtain basic information for the color plan that satisfies their desires. The results of this study are as follows. Firstly, in terms of the color distribution of living spaces, for the ceiling and general walls, results showed that N (white) systems and YR of bright tones were mostly distributed. For the color of the floor, results showed that the calm tone of YR was mostly distributed. Secondly, for the favored colors of finishing materials in each space, the greatest distribution of preference color was found to be the N (white) group (series) in the case of ceilings, For general walls, the bright tone of the N (white) group was the most common in living rooms, and the bright tone of RP was used mostly in couples' rooms and girls' rooms. The bright tone of B and PB were found mostly in boys' bedrooms. In the case of the floor color, results showed the largest distribution was the calm tone of YR. For point walls, the preference was for various kinds of bright and brilliant colors. Thirdly, we could determine that extroverts (E) strongly disliked black and grey, while introverts (I) strongly disliked red as well as black and grey. Those who favored colors of closing materials were the sensible types (S) where colors were concentrated in common color groups. On the other hand, intuition types (N) preferred color groups of various kinds, not only the typically preferred colors.