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Separation and Purification of Angiotensin Converting Enzyme Inhibitory Peptides Derived from Goat's Milk Casein Hydrolysates

  • Lee, K.J.;Kim, S.B.;Ryu, J.S.;Shin, H.S.;Lim, J.W.
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.5
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    • pp.741-746
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    • 2005
  • To investigate the basic information and the possibility of ACE-inhibitory peptides for antihypertension materials, goat's caisin (CN) was hydrolyzed by various proteolytic enzymes and ACE-inhibitory peptides were separated and purified. ACE-inhibition ratios of enzymatic hydrolysates of goat's CN and various characteristics of ACE-inhibitory peptides were determined. ACE-inhibition ratios of goat's CN hydrolysates were shown the highest with 87.84% by pepsin for 48 h. By Sephadex G-25 gel chromatograms, Fraction 3 from goat's CN hydrolysates by pepsin for 48 h was confirmed the highest ACE-inhibition activity. Fraction 3 g and Fraction 3 gh from peptic hydrolysates by RP-HPLC to first and second purification were the highest in ACE-inhibition activity, respectively. The most abundant amino acid was leucine (18.83%) in Fraction 3 gh of ACE-inhibitory peptides after second purification. Amino acid sequence analysis of Fraction 3 gh of ACE-inhibitory peptides was shown that the Ala-Tyr-Phe-Tyr, Pro-Tyr-Tyr and Tyr-Leu. IC$_{50}$ calibrated in peptic hydrolysates at 48 h, Fraction 3, Fraction 3 g and Fraction 3 gh from goat's CN hydrolysates by pepsin for 48 h were 29.89, 3.07, 1.85 and 0.87 g/ml, respectively. Based on the results of this experiment, goat's CN hydrolysates by pepsin were shown to have ACE-inhibitory activity.

Laser Processing for Manufacturing Styrofoam Pattern (주물용 스티로폼 목형 제작을 위한 레이저 가공 공정 개발)

  • 강경호;김재도
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 2001.04a
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    • pp.1085-1088
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    • 2001
  • The process of styrofoam pattern that has been used for material of press die pattern depends chiefly on handwork. Laser manufacturing system developed to increase precision and efficiency of process that is also able to convert the design easily. Applying the RP(rapid prototyping) concept reversely, the unnecessary part of section is vapored away by heat source of laser beam after converting 3-D CAD model into cross-sectional shape information. Laser beam is line-scanned in plane specimens to measure the depth and width of cut, surface roughness, cross-sectional shape as converting laser power, scanning speed, cutting gas pressure. With these basic data, plane surface, inclined surface, hole, outer contour trimming process is experimented and optimum condition are obtained. In plane and inclined surface experiments, 15W laser power and 50mm/s scanning speed make superior processing property and 30W, 10mm/s make processing efficiency increase in trimming process. With these results, simple patterns were manufactured and the possibility of applying laser manufacturing system to styrofoam pattern was convinced.

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Estimation for Retention Factor of Isoflavones in Physico-Chemical Properties

  • Lee, Seung-Ki;Row, Kyung-Ho
    • Bulletin of the Korean Chemical Society
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    • v.24 no.9
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    • pp.1265-1268
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    • 2003
  • The estimation of retention factors by correlation equations with physico-chmical properties maybe helpful in chromatographic work. The physico-chemical properties were water solubility (S), hydrophobicity (P), total energy ($E_t$), connectivity index 1 ($^1{\chi}$), hydrophilic-lipophlic balance (x) and hydrophilic surface area (h) of isoflavones. The retention factors were experimentally measured by RP-HPLC. Especially, the empirical regulations of water solubility and hydrophobicity were expressed in a linear form. The equation between retention factors and various physico-chemical properties of isoflavones was suggested as $k = a_0 + a_1\;log S + a_2log\;P^Q + a_3(E_t) + a_4(^1{\chi}) + a_5(x) + a_6(h)$, and the correlation coefficients estimated were relatively higher than 0.95. The empirical equations might be successfully used for a prediction of the various chromatographic characteristics of substances, with a similar chemical structure.

Molecular Characterization and Expression Analysis of Ribosomal Protein S6 Gene in the Cashmere Goat (Capra hircus)

  • Bao, Wenlei;Hao, Xiyan;Zheng, Xu;Liang, Yan;Chen, Yuhao;Wang, Yanfeng;Wang, Zhigang
    • Asian-Australasian Journal of Animal Sciences
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    • v.26 no.11
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    • pp.1644-1650
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    • 2013
  • Ribosomal protein (rp) S6 is the substrate of ribosomal protein S6K (S6 kinase) and is involved in protein synthesis by mTOR/S6K/S6 signaling pathway. Some S6 cDNA have been cloned in mammals in recent years but has not been identified in the goat. To facilitate such studies, we cloned the cDNA encoding Cashmere goat (Capra hircus) S6 (GenBank accession GU131122) and then detected mRNA expression in seven tissues by real time PCR and protein expression in testis tissue by immunohistochemisty. Sequence analysis indicated that the obtained goat S6 was a 808 bp product, including a 3' untranslated region of 58 bp and an open reading frame of 750 bp which predicted a protein of 249 amino acids. The predicted amino acid sequence was highly homologous to cattle, human, mouse and rat S6. Expression analysis indicated S6 mRNA was expressed extensively in detected tissues and S6 protein was expressed in testis tissue.

Endothelin-1 Levels in Patients with Heart Disease Associated with Pulmonary Hypertension ; Potential role of Endothelin-1 in genesis of pulmonary artery vasospasm (개심술 환자에서 술전및 술후의 폐동맥 고혈압및 혈역학적 변수들과 ET-1치와의 관계에 대한 연구)

  • 박형주
    • Journal of Chest Surgery
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    • v.25 no.6
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    • pp.650-660
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    • 1992
  • To elucidate a potential contribution of endotheline-1[ET-1] to the genesis of pulmonary hypertension and postoperative pulmonary hypertensive crisis in the patients with heart disease, we measured plasma levels of the ET-1 during perioperative period of open heart surgery. In addition, we examined changes of ET-1 during perioperative period and correlations between ET-1 levels and hemodynamic variables. 12 patients including 5 acquired heart disease and 7 congenital heart disease patients were selected randomly as a study group, Group A and B, respectively. 6 patients proved not having heart or hemodynamic problem were selected as a control, Group C. 110 blood samples from pulmonary artery[ET-P] and radial artery[ET-S] were taken and assayed by Sep-pak extraction and RIA. ET-1 levels of Group A were ET-P, 3.94$\pm$5.31pg /ml, ET-S, 3.10$\pm$2.90pg/ml[p>0.05], Group B were ET-P, 1.63$\pm$0.62pg/ml, ET-S, 1.99$\pm$2.45pg/ml[p>0.05], Group C were ET-P, 1.97$\pm$2.02pg/ml, ET-S, 1.72$\pm$0.77pg/ml[p>0.05]. There were no statistically significant differences of ET-1 levels among the Group A, B, C[p>0.05]. There was no correlation between pulmonary artery pressure[PAP] and ET-1 level[p>0.05], and ET-1 levels were not increased even in the cases of pulmonary hypertensive criwis or low cardiac output syndrome, whereas significant correlation between ET-S and pulmonary vascular res-istance[Rp] [r=0.36, p<0.05], and negative correlation between ET-S and OS saturation of pulmonary artery[OS-P][r= -0.49, p<0.01] were identified. Another significant finding was peak increase of ET-1 levels in the postoperative period 1 hour[p<0.05] and then gra-dualy decrease through the postoperative period. In conclusion, ET-1 has no correlation with PAP, whereas correlation with Rp, and inverse correlation with OS-P. It is suggested that ET-1 is neither the direct causative substance of pulmonary hypertension nor pulmonary vasospasm but there must be increased production of ET-1 in chronic pulmonary hypertensive state. Counter-regulatory mechanism to ET-1 is speculated during the pulmonary vasospasm.

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A Study on the Design Characteristics of Chanel Bags - focused on the collections from S/S 2001 to F/W 2008 -

  • Jang, Ji-Hye;Cho, Kyu-Wha
    • Journal of Fashion Business
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    • v.12 no.6
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    • pp.93-106
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    • 2008
  • The purpose of this study is to analyze design characteristics of Chanel bags by its type and shape, material, color, pattern, decorations in order to give information about foundation of the development of Korean fashion brand handbags and help suggest predict future handbag trends. The methods of this study are documentary research and demonstrative research. For the documentary research, mainly previous researches and fashion related data were used. For the demonstrative research, the total of 288 design photos of Chanel bag were selected from 2001 S/S season to 2008 F/W season fashion collections of firstview.com., style.com., and mode et mode. The results of this study are as follows; First, type and shape of bags are clutch(30%), flap(25%), shoulder(25%), others(10%), tote(5%), hobo(5%). Second, the material data shows that leather(46%), mixed(18%), fabric(17%), synthetics (10%), patent(3%), others(3%), and suede(2%). Third, the patterns are geometrical(27%), solid(25%), combination(24%), symbolic(11%), abstract(11%), nature(2%). Fourth, the colors are largely monochrome(73%) and multicolor(27%). In case of monochrome, achromatic color(50%) is more than chromatic color(23%). The chromatic color is consisted of YR(9%), Y(9%), R(5%), RP(4%), PB(2%), P(1%), GY(0.5%), BG(0.5%). The multicolor is consisted of similar coloration(12%), contrast coloration(10%), and accent coloration(5%). Fifth, the decoration data shows that metallic(53%), plain(23%), combination(22%), and handcrafted(1%).

Properties and Hair-growth Effect of Chrysin 7-O-crotonate (크라이신 7-O-크로토네이트의 물성 및 육모효과)

  • 신준수;김연희;정재훈;김양배;김박광
    • YAKHAK HOEJI
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    • v.43 no.3
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    • pp.316-319
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    • 1999
  • Chrysin 7-Ο-crotonate was synthesized by condensing crotonic acid with chrysin in organic solvent, tetrahydrofuran (THF) using dicyclohexylcarbodiimide (DCC) and 4-dimethylaminopyridine (DMAP). Its structure was indentified by NMR, MS, UV, IR etc. We also investigated the physico-chemical properties and set up the quantitative anytical method of this compound. The correlation coefficient of calibration curve measured at the isobestic point (340 nm) on this compound was approximately 0.9994 by absorption spetrophtometry. Detection limit was 1.6ng at S/N=3 by using a RP column by HPLC. The hair growth effect fo chrysin 7-Ο-crotonate on the hair of black mouse (C57BL/6), was carried out using paste method. When its ethanol solution was administered to this black mouse by route of skin, this compound promoted the growth of hair.

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Comparison of Phytochemical and Antioxidant Activities in Different Color Stages and Varieties of Paprika Harvested in Korea (국내산 파프리카의 색상별 phytochemical 및 항산화능 비교)

  • Kim, Ji-Sun;Ahn, Ji-Yun;Ha, Tae-Youl;Rhee, Han-Cheol;Kim, Sun-A
    • Korean Journal of Food Science and Technology
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    • v.43 no.5
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    • pp.564-569
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    • 2011
  • This study qualitatively and quantitatively analyzed various carotenoids, L-ascorbic acid, and tocopherol and their anti-oxidant properties from four varieties (Special, Chelsea, Cupra, and Fiesta) and three different color stages [green (GP), yellow (YP) and red (RP)] of paprika harvested in Korea. Seven carotenoids were identified, mainly lutein in GP (Special: 4.65${\pm}$0.84 mg/kg fresh weight (fw)) and YP (Fiesta: 5.19${\pm}$0.05 mg/kg fw), and capsorubin (3.16${\pm}$0.35 mg/kg fw) and capsanthin (53.70${\pm}$6.23 mg/kg fw) in Special of RP. RP was the highest in total carotenoids, L-ascorbic acid, and ${\alpha}$-tocopherol contents, while GP was the highest in ${\gamma}$-tocopherol content. RP showed the strongest antioxidant activity ($IC_{50}$=62.40${\pm}$0.03 ${\mu}g/mL$ in an ABTS assay and 182.77${\pm}$31.74 ${\mu}g/mL$ in a DPPH assay). Paprika in different color stages has many phytochemicals even though they have different kinds of carotenoids. Therefore, dietary intake of paprika may be helpful for improving human health.

Purification and Characterization of the Bacteriocin Produced by Lactococcus sp. KD 28 Isolated from Kimchi (김치에서 분리한 Lactococcus lactis가 생산하는 박테리오신의 정제 및 특성)

  • Lee, Ji-Young;Choi, Nack-Shick;Chun, Sung-Sik;Moon, Ja-Young;Kang, Dae-Ook
    • Journal of Life Science
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    • v.25 no.2
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    • pp.180-188
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    • 2015
  • The bacterial strain isolated from Kimchi showed antibacterial activity against Micrococcus luteus IAM 1056. The selected strain was identified as Lactococcus lactis by 16S rRNA nucleotide sequence analysis and named as Lactococcus sp. KD 28. The treatment of culture supernatant with proteinase K removed antibacterial activity, indicating its proteinaceous nature, a bacteriocin. This bacteriocin was sensitive to hydrolytic enzymes such as ${\alpha}$-chymotrypsion, trypsin, proteinase K, lipase, ${\alpha}$-amylase and subtilisin A. The bacteriocin was highly thermostable and resistant to heating at $80^{\circ}C$ for up to an hour but 50 % of the total activity was remained at $100^{\circ}C$ for 30 min. The pH range from 2.0 to 8.0 had no effect on bacteriocin activity and it was not affected by solvents such as acetonitrile, isopropanol, methanol, chloroform and acetone up to 50% concentration. The bacteriocin showed antibacterial activity against M. luteus IAM 1056, Lactobacillus delbrueckii subsp. lactis KCTC 1058, Enterococcus faecium KCTC 3095, Bacillus cereus KCTC 1013, B. subtilis KCTC 1023, Listeria ivanovii subsp. ivanovii KCTC 3444, Staphylococcus aureus subsp. aureus KCTC 1916, B. megaterium KCTC 1098 and B. sphaericus KCTC 1184. The bacteriocin was purified through ammonium sulfate concentration, SP-Sepharose chromatography and RP-HPLC. The molecular weight was estimated to be about 3.4 kDa by tricine-SDS-PAGE analysis.

Inhibition of Red Ginseng on 5-Hydroxyeicosatetraenoic Acid (5-HETE) Biosynthesis from Arachidonic Acid in Helicobacter Pylori-infected Gastric Cells

  • Park Soo-Jin
    • Nutritional Sciences
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    • v.9 no.3
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    • pp.152-158
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    • 2006
  • Helicobacter pylori (H. pylori) infection rapidly stimulated either COX-2 or 5-LOX and released arachidonic acid metabolites that have been considered as pivotal mediators in H. pylori-induced inflammatory responses. To determine whether red ginseng extract (RGE) can suppress the biosynthesis of 5(S)-hydroxyeicosatetraenoic acids (HETE), a precursor metabolite of leukotrienes B4 (LTB4) in H. pylori-provoked inflammatory responses in gastric epithelial cells, the biosynthesis of monohydroxy fatty acids was measured using radioactive arachidonic acid and validated by RP-HPLC using non-radioactive AA as substrate in AGS cells cocultured with H. pylori (ATCC 43504) with or without pretreatment of RGE. Among three known major HETEs, H. pylori infection specifically induced the biosynthesis of $^{14}C-5(S)-HETE$ rather than the complex of $^{14}C-15S-/^{14}C-12(S)-HETE$ from $^{14}C-AA$, concomitantly obtained by HPLC(p<0.01). RGE, 1 to $100{\mu}g/ml$, selectively suppressed H. pylori-stimulated $^{14}C-5(S)-HETE$ production implying the attenuation of 5-lipoxygenase activity, of which was similar to known LOX inhibitor NDGA $(10{\mu}M)$ (p<0.01). However, the amount of 5(S)-HETE was significantly reduced by higher dose of RGE $(100{\mu}g/ml)$ (p<0.05). These results indicated that LOX pathway might be one of principle pathogenic mechanisms of H. pylori and red ginseng could be a nutraceutical against H. pylori infection through inhibiting action of LOX activity.