• 제목/요약/키워드: Roundup

검색결과 17건 처리시간 0.017초

새포아풀 방제를 위한 Roundup Ready Bentgrass의 개발 (Development of Roundup Ready Bentgrass for the Control of Poa annua)

  • 닉크리스챤스;이정호
    • 아시안잔디학회지
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    • 제20권1호
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    • pp.77-82
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    • 2006
  • 애뉴얼 블루그래스는 골프장에서 방제하기가 대단히 어려운 잡초이다. 방제가 어려운 이유로는 애뉴얼 블루그래스의 경우 불량환경에서도 왕성하게 종자생산을 할 수 있는 특성과 또한 낮은 예초환경에서도 적응력이 있기 때문이다. 하지만 무엇보다도 유전적으로 다양성이 있기 때문에 골프장의 잔디에 비해 우점할 수 있는 것이다. 일반적으로 애뉴얼 블루그래스는 일년생 한지형 잔디로, 생육형은 주형이다. 하지만 포복경이 있어 다년생의 특성을 갖는 생태형이 다른 애뉴얼 블루그래스도 있다. 골프장에서 애뉴얼 블루그래스 방제를 위해 화학적 방법과 재배적 방법에 관한 수많은 연구가 지난 85년간 진행되었다. 연구결과 일부 종류의 애뉴얼 블루그래스 방제에 성공적인 결과도 있지만, 모든 종류의 애뉴얼 블루그래스 방제에 성공적인 방법은 아직 없는 실정이다. 이 모든 방제 방법들 중에 Roundup ready creeping bentgrass의 사용은 다양한 종류의 새포아풀을 방제 할 수 있다. Roundup readgy bentgrass는 Roundup에 내성이 있어 약해 피해 없이 생존이 가능하다. 따라서 골프장에서 이 새로운 기술을 활용하면 새포아풀이 없는 그린, 티, 훼어웨이를 유지할 수 있다.

Strategic Cattle Roundup using Multiple Quadrotor UAVs

  • Jung, Sunghun;Ariyur, Kartik B.
    • International Journal of Aeronautical and Space Sciences
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    • 제18권2호
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    • pp.315-326
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    • 2017
  • Four quadrotor UAVs are maneuvered to guide four animals into their pen within the minimum time by creating noises of predators modeled with an exponential function. The quadrotor UAVs are controlled via PID controllers, follow time optimal trajectories, and avoid collisions through altitude separations. The stability of the proposed PID controller is analyzed and verified using MATLAB/Simulink based simulations. Proposed step by step strategies would be practical solutions of actual cattle roundup problems.

Roundup BioKorea -Workshop on Guiding Research Products to Commercial BioProducts-

  • Jung, Youn-Chan
    • 대한약학회:학술대회논문집
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    • 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-1
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    • pp.25-25
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    • 2003
  • “Roundup BioKorea” is the project to develop the role of Korea Food and Drug Administration in leading the process of the effective materials from basic research work to licensed commercial BioProducts. Korean government has invested a large amount of budget to a biotechnological area by “Biotech 2000” program since 1994. (omitted)

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제초제처리(除草劑処理)에 의(依)한 잣나무털녹병(病) 중간기주(中間奇主)(송이풀류(類)) 제거시험(除去試験) (Studies on the Eradication of Pedicularis spp., Alternate Host of White Pine Blister Rust with Several Herbicides)

  • 이창근;김현중;여운홍
    • 한국산림과학회지
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    • 제51권1호
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    • pp.51-55
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    • 1981
  • 우리나라 잣나무털녹병(病)의 중간기주(中間寄主)인 송이풀류(類)를 제거(除去)하기 위(爲)하여 1975년(年)부터 몇 가지 제초제(除草劑)을 산지(山地)에 자생(自生)하는 송이풀류(類)에 처리(處理)하여 다음의 결과(結果)를 얻었다. 1. Roundup은 주위(周圍)의 초(草), 목본류(木本類)에 약해(薬害)를 주었으나 송이풀제거용(除去用) 제초제(除草劑)로서 가장 효과(効果)가 있었다. 2. Roundup의 송이풀 제거효과(除去効果)는 처리(處理) 1 년후(年後)에 100%, 2 년후(年後)에는 65~92%의 밀도감소효과(密度減少効果)가 있었다. 3. 2.4-D, Gramoxone, 2. 4. 5-T 및 Para-col은 처리당년도(處理當年度)에 송이풀의 경엽제거(茎葉除去)는 가능(可能)하였으나 익년도(翌年度) 봄에 재발순(再發筍)되었다. 4. 제초제(除草劑)의 살포시기(撒布時期)는 5~6月(송이풀발순완료기(發筍完了期))보다 완전(完全)히 성숙(成熟)된 8월하순(月下旬)에 살포(撒布)하는 것이 효과적(効果的)이였다.

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Effect of a fall cut on dry matter yield, nutritive value, and stand persistence of alfalfa

  • McDonald, Iryna;Min, Doohong;Baral, Rudra
    • Journal of Animal Science and Technology
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    • 제63권4호
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    • pp.799-814
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    • 2021
  • Information is lacking about the effect of date of a fall cut of alfalfa (Medicago sativa L.) on dry matter yield (DMY), forage nutritive value, and stands persistence. The objective of this study was to determine the effect of timing of a fall cut on DMY, forage nutritive value and stand persistence of three alfalfa varieties: low-lignin Hi-Gest 360, Roundup Ready Tonica, and conventional Gunner in Northeastern Kansas in the United States. The field study was carried out by splitting plot in randomized complete block design with four replications. The harvesting data of different maturity stages were collected in each year from 2015 to 2018. Three cuts were harvested based on the stage of maturity, and the last (fourth) cut was done on September 15, September 30, October 15, and October 30 of each year. The persistence of the alfalfa stands was determined each fall after the last cut, and each spring after the first cut, by counting the number of live plants in a randomly placed quadrat in each plot. Alfalfa cut on September 15 and September 30 had a higher stand persistence compared to alfalfa cut on October 15 and October 30. The DMY of the first cut in 2016 was significantly higher in roundup ready than the low-lignin alfalfa variety. In the second cut, DMY was significantly higher in conventional alfalfa than the roundup ready. There were no significant differences in DMY between alfalfa varieties in the rest of seasonal cuttings in 2016 and 2017 and annual total yield in both years. In general, low lignin alfalfa variety had higher crude protein and relative feed value and lower acid detergent fiber and neutral detergent fiber contents than those in roundup ready and conventional alfalfa varieties. On average, nutritive value of alfalfa was generally affected by last cutting dates in 2017. Based on 3-year data the last cutting of alfalfa in the fall could be done by September 30-October 15 without harmful effect on DMY.

유전자변형 콩의 검정법 (Detection Methods for Genetically Modified Soybeans)

  • 손성한;정순일;윤문섭;김태산;박용환;김영미
    • Applied Biological Chemistry
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    • 제45권4호
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    • pp.185-189
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    • 2002
  • 우리나라의 유전자변형농산물 의무 표시제가 시행됨에 따라 수입 유전자변형농산물 중 유전자변형 콩의 혼입유무를 판별할 수 있는 검정기술 개발이 요구되고 있다. 근사미(glyphosate)제초제에 저항성을 나타내는 토양미생물인 Agrobacterium CP4 유래의 5-enolpyruvyl shikimate-3-phosphate synthase(EPSPS) 유전자의 도입여부를 PCR로 진단할 수 있는 특이프라이머를 제작하여 제초제저항성 콩(Roundup Ready Soybean, RRS)을 검정할 수 있는 PCR조건을 확립하였으며 콩의 내재유전자인 lectin유전자와 RRS특이 프라이머를 이용하여 duplex PCR에 의한 제초제저항성 콩의 검정법을 확립하였다. 또한 수입 콩 및 콩나물에 대하여 근사미 제초제 처리로 저항성 개체를 판별하는 생물검정법도 확립하여 저항성 개체의 잎에서 분리한 genomic DNA에 대하여 EPSPS특이 프라이머를 이용하여 분석한 결과 RRS특이적인 PCR밴드를 확인하였다. 또한 수입 콩의 백립중과 종실의 제색을 고려할 때 단일품종이 아닌 여러 품종이 혼합되어 있음을 확인하였다.

유전자변형 콩과 자연 콩의 알레르기 유발원 비교 (Comparison of Allergens in Genetically Modified Soybean with Conventional Soybean)

  • 박재현;정승태;김재희;김지영;노건웅
    • 약학회지
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    • 제45권3호
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    • pp.293-301
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    • 2001
  • Genetically modified organism (GMO) using recombinant DNA technique has been exponentially increased, however there are still arguments for the safety of GM foods. The objective of this research was to compare the allergens of GM soybean(Roundup Ready$^{TM}$) with conventional soybeans. Each soybean extracts were prepared as crude extracts, heated extracts, and as heated and simulated gastric quid (SGF)-digested samples to characterize the stability of allergens to physicochemical treatment. Positive sera from 20 soybean-sensitive patients and control sera from 5 normal subjects were used to identify the endogenous allergens in soybeans. Specific-IgE binding activities to each soybean preparations were evaluated by ELISA and immunoblot technique. In ELISA result, IgE binding activities of positive sera to soy crude extracts generally showed two fold higher mean value than those of control sera, how-ever there was no significant difference between GM soybean and natural soybean varieties. Extracted proteins form each of the soybean preparations were separated with SDS-PAGE. The band pattern of GM soybean was very similar to those of natural soybean varieties. Immunoblots for the different soybeans revealed no differences in IgE-binding protein patterns, moreover, disclosed five prominent IgE-binding bands (75, 70, 50, 44 and 34 kDa) in crude extracts, four (75, 70, 44 and 34 kDa) in heated preparations, one (50 kDa) in heated and SGF-digested preparations. These IgE binding bands were consistent with previously reported results on the soybean. These results indicate that GM soybean (Roundup Ready$^{TM}$) is no different from natural soybean in terms of its allergen.gen.

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A Simple and Reliable Method for Preparation of Cross-Contamination-Free Plant Genomic DNA for PCR-Based Detection of Transgenes

  • Hwang, Seon-Kap;Kim, Young-Mi
    • BMB Reports
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    • 제33권6호
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    • pp.537-540
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    • 2000
  • A simplified but reliable method was developed for the polymerase chain reaction (PCR)-based detection of genetically modified (GM) plants. The modified CTAB (mCTAB) method enabled us to prepare a high quality of genomic DNA from several hundred plant leaf samples in one day. Using DNA samples prepared from seven dicots and two monocots, approximately 1.75-kb regions spanning 17 S to 25 S ribosomal RNA genes were successfully amplified in a 2X PCR pre-mix containing BLOTTO. Further fidelity assessment of the mCTAB method by PCR analysis with Roundup Ready soybean (RRS) and non-RRS plants showed that the DNA samples prepared alternately from each of two lines were evidently free of cross-contamination. These results demonstrate that the mCTAB method is highly recommended for the rapid detection of transgenes in large numbers of leaf samples from diverse transgenic plants.

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식품 중 제초제내성 유전자재조합 콩의 immunoblotting 분석 (Immunoblotting Assay for Glyphosate-tolerant Genetically Modified Soybean in Soybean Products)

  • 손대열;문정희;안강모;손동화;이광신;심희영;한영신;이상일
    • 한국식품과학회지
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    • 제36권3호
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    • pp.369-374
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    • 2004
  • 유전자재조합 콩 Roundup Ready는 Agrobacterium sp. CP4에서 유래한 유전자를 함유하고 있으며 이 삽입 유전자에서 5-enolpyruvylshikimate-3-phosphate synthase(CP4EPSPS)가 발현된다. 본 연구실에서는 이 단백질을 유전자재조합 콩과 시중에서 구입한 두부 시료에서 CP4EPSPS 특이 단클론 및 다클론 항체를 이용하여 immunoblotting법으로 검사하였다. 분리 정제된 CP4EPSPS 재조합 단백질은 단클론 또는 다클론 항체를 이용할 때 각각 $0.006{\mu}g$ 또는 $0.0006{\mu}g$의 검증 한계치로 확인할 수 있었다. 유전자재조합 콩에 발현된 CP4EPSPS 검증 한계치는 단클론 또는 다클론 항체를 이용할 때 각각 $0.001{\mu}g$$0.0001{\mu}g$이었다. 다클론 항체를 이용하여 조사된 9종의 두부에서는 2종에서 양성결과를 확인하였다. 본 연구에서 생산된 단클론 및 다클론 항체를 이용하여 확립된 immunoblotting법은 콩 가공식품 중의 glyphosate 내성 유전자재조합 콩 검사에 적용될 수 있을 것이다.