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Effects of Salviae Miltiorrhizae Radix Hot Aqueous Extract on Nitric Oxide and Prostaglandin E2 Production and on 1,1-diphenyl-2-picryl hydrazyl Radical Scavenging in Macrophages

  • Yeo, In Ho;Lee, Cham Kyul;Lee, Eun Yong
    • Journal of Pharmacopuncture
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    • v.17 no.1
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    • pp.7-12
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    • 2014
  • Objectives: The objective of this study is to investigate the effects of Salviae Miltiorrhizae Radix hot aqueous extract on nitric oxide (NO) and prostaglandin $E_2$ ($PGE_2$) production and on 1,1-diphenyl-2-picryl hydrazyl (DPPH) free-radical scavenging in macrophages. Methods: Salviae Miltiorrhizae Radix (300 g) was heated at $100^{\circ}C$ with distilled water (2 L) for 4 hours. The extract was filtered and concentrated to 100 mL by using a rotary evaporator, was frozen at $-80^{\circ}C$, and was then freeze-dried by using a freezing-drying system. The RAW 264.7 macrophage was subcultured by using $10-{\mu}g/mL$ lipopolysaccharide (LPS). In order to evaluate cytotoxicity, we performed 3-(4,5-dimrthylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays and measured the cell viability. The NO production was measured by using Griess assays, and the $PGE_2$ production was measured by using enzyme immunoassays. The antioxidant activity, the 1,1-diphenyl-2-picryl hydrazyl (DPPH) free-radical scavenging capability, was measured by using the DPPH method. Results: Cell viability with the 1-, 5-, 25-, 125- and $625-{\mu}g/mL$ Salviae Miltiorrhizae Radix hot aqueous extract was not significantly decreased compared to the cell viability without the extract. When 125 and $625{\mu}g/mL$ of Salviae Miltiorrhizae Radix hot aqueous extract were used, nitric oxide (NO) production in LPS-stimulated RAW 264.7 macrophages was significantly inhibited compared to that in the control group. When 25, 125, and $625{\mu}g/mL$ of Salviae Miltiorrhizae Radix hot aqueous extract were used, $PGE_2$ production in LPS-stimulated RAW 264.7 macrophages was significantly inhibited compared to that in the control group. The 125- and $625-{\mu}g/mL$ Salviae Miltiorrhizae Radix hot aqueous extracts had high DPPH free-radical scavenging capabilities in RAW 264.7 macrophages. Conclusion: This study indicates that Salviae Miltiorrhizae Radix hot aqueous extract suppresses NO and $PGE_2$ production and improves DPPH free-radical scavenging capability. Thus, it seems that Salviae Miltiorrhizae Radix hot aqueous extract may have an anti-inflammation effect and antioxidant activity.

UHV Materials (초고진공계재료)

  • 박동수
    • Proceedings of the Korean Vacuum Society Conference
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    • 1998.02a
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    • pp.24-24
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    • 1998
  • 반도체장비를 포함하는 초고진공장비의 園훌化가 급속히 그리고 절실히 요구되고 있는 것이 현실정이다. 當面해서 실현할 국산진공장비의 대상은 廣範圍하다. 즉, 각종 진공 pump ( (rotary, dry, diffusion, cryo, ion, turbo melecular pump), 진공 chamber, 진공 line, gate valve 를 위 시 한 진공 V머ve, flange, gasket, fl않d야lU, mainpulater 퉁 진공 部品이 다. 진공계 의 핵심 은 適切하고 優良한 진공재료의 선태파 사용이다. 진공장비는 사용자가 원하는 진공도를 원하 는 시간 동안 륨空度를 유지해 주어야 한다. 진공재료 선태의 기준사항은:(1) 기체의 透過성 (2) 薰했훌 (3) 혔體放出특성 - -outgassing과 degassing- (4) 機械的 량훌度 (5) 온도 의존성 (6) 化學톡성 (7) 加I성 및 鎔接 성 (8) 課電특성 (9) 磁氣특성 (10) 高速함子 및 放射線 특성 (11) 經濟성 및 調達생 둥이 다. 우량한 초고진공계재료는 풍부하게 개발되어 왔고, 또 新材料들이 개발되고 있다. 여기에서는 주로 초고진공 내지는 극고진공계의 構造材料, 機能材料, 部品材料 일반파 몇가지 신재료의 특 성에 관해서 記述한다. M Mild SteeHSAE, 1112, 1010, 1020, 1022, etc)., S Stainless SteeHAlSI, 304, 304L, 310, 316, 321, 347): 구조재료, chamber, fl하1ges A Aluminum과 Alloys (1060, 1100, 2014, 4032, 6(뻐1): 구조재료, chamber, flanges, gaskets A AI, Al 떠loy는 SS에 代替하는 역 할올 시 작하고 있다. C Copper, Copper Alloys(C11$\alpha$)0, C26800, C61400, Cl7200): 내장인자, gasket, cryopanel, tubing T Titanium, Ziriconium, Haf띠um 및 Alloys: 특히 Ti은 10n pump 용 getter material 이 외 에 U UHV,XHV용 chamber계로서 관심올 끌고 있다. N Nickel, Nickel Alloys (200, 204, 211, monel, nichrome): 부식 방지 , 전자장치 , 자기 장치 귀 금속(Ag, Au, Pt, Pd, Rh, Ir, Os, Ru): 보조부품, gasket, filament, coating, thermocouple, 접 합부위 T TiC, SiC, zrC, HfC, TaC 둥의 탄화물과, BN, TiN, AlN 동의 질화물, 붕화물이 둥장하고 었 다. 유리: Soda Lime, Borosilicate, Potash Soda Lead: View Port, Chamber envelope C Ceramics: AlZ03, BeO, MgO, zrOz, SiOz, MgOzSiOz, 3Alz032SiOz, Z$textsc{k}$hSiOz S상N4: e electrical, thermal insulators, crucibles, boats, single crystals, sepctr려 windows 저자는 최근 저자들이 발견한 Zr-Ti-Cu-Ni-Be amorphous alloys coated cham뾰r가 radiation p proof로 이용될 수 있는 사실을 점검하고 었다 .. Z.Y. Hua 들은 Cs3Sb를 새로운 photocathode 재료로 보고하고 있다.

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Control of Weedy Rice Using Minimum Tillage Direct Seeding of Rice on Dry Paddy (벼 최소경운 건답직파재배를 이용한 잡초성벼 방제)

  • Seong, Ki-Yeong;Lee, Jeong-Tae;Park, Tae-Seon;Cho, Hyeon-Suk;Seo, Myung-Chul;Kim, Mi-Hyang;Kang, Hang-Won
    • Weed & Turfgrass Science
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    • v.2 no.3
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    • pp.322-326
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    • 2013
  • This study was tried to control the weedy rice using minimum tillage direct seeding of rice on dry paddy. Minimum tillage direct seeding on dry paddy is tilled with 4 cm in width at 27 cm intervals by 1-shaped rotary blade. Using this cultivation method can control weedy rice effectively by minimizing the soil disturbance. In this study, weedy rice was firstly emerged at April 28 in 2012. After 11 days emergence rate of weedy rice at May 9 was 61.9%. At this time rice was sown and then herbicides was treated at 10 and 15 days after sowing. When herbicides was treated at 10 days after sowing the control effect was 89-96%, and when treated at 15 days the control effect was 97-99%. However emergence of weedy rice differ according to the year and year. It was thought to be affected by April average temperature and total precipitation. So, for effective weedy rice control and stable rice yield, it was considered that irrigation at April 15-20 to induce the emergence of weedy rice is required.

Physicochemical Changes of Woody Charcoals Prepared by Different Carbonizing Temperature (탄화온도가 목탄의 물리·화학적 특성에 미치는 영향)

  • Jo, Tae-Su;Choi, Joon-Weon;Lee, Oh-Kyu
    • Journal of the Korean Wood Science and Technology
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    • v.35 no.3
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    • pp.53-60
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    • 2007
  • Carbon content, properties of micro-pore, and chemical properties of the charcoal prepared from wood powder, wood fiber, and bark of Abies sibirica Ledeb at different temperatures were investigated. The yield of charcoal decreased with increasing the carbonization temperature. The yield of bark charcoal was higher than those of wood and wood fiber charcoal. The content ratio of carbon atom in the charcoal increased with increasing the carbonization temperature, whereas those of hydrogen and oxygen atom were decreased. Ash content of bark charcoal was also higher than those of wood and wood fiber charcoal. The specific surface area of wood and wood fiber charcoal was greater than that of bark charcoal. In all charcoal, the specific surface area and the volume of micro-pore were highest when the carbonization temperature was $600^{\circ}C$, however they tended to decrease when the temperature was reached to $800^{\circ}C$. For the functionality test of chemical groups on the charcoal surface, adsorption test have performed against acidic (HCl) and basic chemicals (NaOH, $Na_2CO_3$, and $NaHCO_3$). As carbonization temperature increased, adsorption amount of HCl increased, while adsorption amounts of NaOH, $Na_2CO_3$, and $NaHCO_3$ were decreased. The charcoal prepared at higher temperature showed basic properties, while the charcoals manufactured at lower temperature presented acidic properties. Therefore, it was considered that the carbonization temperature affected the pH of charcoal.

Changes in biological activities and nutritional contents of edible mushrooms following roasting treatment (로스팅 처리에 따른 식용버섯의 생리활성 및 영양성분 변화)

  • An, Gi-Hong;Han, Jae-Gu;Cho, Jae-Han
    • Journal of Mushroom
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    • v.18 no.1
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    • pp.63-71
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    • 2020
  • The present study aimed to investigate the biological activities and nutritional contents of extracts from three types of edible mushrooms (Pleurotus ostreatus, P. eryngii, and Hericium erinaceus) following different roasting treatments. Roasting by a household multi-oven (HR60) decreased the total polyphenol contents, as well as DPPH radical scavenging and nitrite scavenging activities compared with the fresh samples, and no significant difference was observed between the different roasting times. As a result of rotary drum roasting (CR) treatment, we could observe a decrease in the DPPH radical scavenging activity in all roasted mushroom samples compared with the fresh samples, whereas nitrite scavenging activity increased in roasted P. eryngii and H. erinaceus. Additionally, total polyphenol contents increased significantly in roasted H. erinaceus. In each roasted sample dried by HR60 and CR, the amount of palatable-taste amino acids (e.g., glutamic acid) and sweet-taste amino acids, including glycine, serine, alanine, and threonine, increased in P. ostreatus, whereas the amount of bitter amino acids, including leucine, isoleucine, histidine, valine, and methionine, decreased compared with the fresh samples. In P. eryngii and H. erinaceus samples, we also observed an increase in the amount of palatable and sweet-taste amino acids upon the roasting treatments; however, the amount of bitter amino acids increased simultaneously following the roasting treatments. These results suggest that CR treatment is more effective in increasing the biological activities and nutritional contents, such as palatable and sweet-taste amino acids, compared with HR60 treatment.

Evaluation of Mechanical Properties and Fatigue Behavior of STS 304L due to Plastic Working (소성가공에 따른 STS 304L 재료의 기계적 특성 및 피로평가)

  • Shim, Hyun-Bo;Kim, Young-Kyun;Suh, Chang-Min
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.41 no.7
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    • pp.635-643
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    • 2017
  • The purpose of this study is to investigate the influence of the cold reduction rate and an ultrasonic fatigue test (UFT) on the fatigue behaviors of STS 304L. The tensile strength, yield strength, hardness value and fatigue limit in the UFT fatigue test linearly increased as thickness decreased from 1.5 mm to 1.1 mm, as the cold reduction rate of STS 304L increased. As a result of the UFT fatigue test (R = -1) of four specimens, the fatigue limit of the S-N curve formed a knee point in the region of $10^6$, and the 2nd fatigue limit caused by giga cycle fatigue did not appeared. In the case of t = 1.1 mm, the highest fatigue limit was 345 MPa, which was 64.3% higher than the original material (t = 1.5 mm). As a result of the UFT fatigue test of STS 304L, many small surface cracks occurred, grown, coalesced while tearing.

Study on VHCF Fatigue Behaviors and UNSM Effects of Hydrogen Attacked STS 316L (수소취화된 스테인리스강 316L의 VHCF(Very High Cycle Fatigue) 피로특성과 UNSM 효과에 관한 연구)

  • Nahm, Seung-Hoon;Baek, Un-Bong;Suh, Chang-Min;Pyun, Young-Sik
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.41 no.11
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    • pp.1011-1020
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    • 2017
  • This study was conducted to investigate the material properties of stainless steel 316L specimens of untreated and UNSM treated material, hydrogen attacked material(100 bar, $300^{\circ}C$ at 120 h) and UNSM treated hydrogen attacked material at room temperature. Results demonstrated that the hydrogen attacked materials showed a tendency toward a slightly decreased fatigue strength, while the hydrogen embrittlement effect was smaller than the S-N curve of conventional untreated material. As compared to untreated material, the fatigue limit of the UNSM treated material increased by 43.8%, while it was 57.1% higher in the UNSM treated hydrogen attacked material than in untreated hydrogen attacked material. The plastic deformation layer was ${\sim}152{\mu}m$ thick, as confirmed by maps showing the level of local plastic deformation affected by the UNSM treatment in three ways: an image quality map, inverse pole figure map, and kernel average misorientation map captured via electron back scatter diffraction. Owing to hydrogen embrittlement, about 90% of surface cracks were smaller than the average grain size of $35{\mu}m$.

The Optimal Condition and Enzyme Activity of Entomopathogenic Fungus Beauveria bassiana Using Extracted Rice Bran (미강추출물을 이용한 곤충병원성 곰팡이 Beauveria bassiana의 최적 배양조건 및 효소활성)

  • Kim, Chang-Su;Lee, Jung-Bok;Kim, Beam-Soo;Lee, Min-Hye;Kang, Kyeong-Muk;Joo, Woo-Hong;Kim, Jin-Won;Im, Dae-Joon;Kwon, Gi-Seok
    • Journal of Life Science
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    • v.23 no.8
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    • pp.1010-1018
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    • 2013
  • The greenhouse whitefly, Bemisia tabaci, is considered one of the most destructive pests of crops. In this study, we aimed to determine the optimal liquid culture conditions in shake flasks for maximal sporulation of Beauveria bassiana M130 using rice bran. The optimal initial pH for the spore production of B. bassiana using extracted rice bran medium was 5.2 and $28^{\circ}C$. The screening in shake flasks of carbon and nitrogen sources resulted in the identification of an optimal medium based on 0.5% $(NH_4)_2SO_4$, with extracted rice bran 8:1. Using this medium, a production level of $2.15{\times}10^9$ spores per ml was obtained after six days from culture inoculation at $28^{\circ}C$ in a rotary shaking incubator at 130 rpm. In addition, the specific activities of extracellular enzymes of chitinase and protease were $4,296{\mu}mol$ and $375{\mu}mol$, respectively. These results suggest that Beauveria bassiana M130 could be a bio-controller for the greenhouse whitefly.

Seasonal Field Assessment of Odor Emitted from Livestock Manure Composting Facility (가축분뇨 자원화시설에서 발생되는 암모니아와 황화수소의 계절별 현장 평가)

  • Kim, Ki Youn
    • Journal of Animal Environmental Science
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    • v.21 no.2
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    • pp.41-46
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    • 2015
  • The objective of this study is to measure the levels of ammonia and hydrogen sulfide in livestock manure composting facility according to seasonal condition. Mean concentrations of ammonia in livestock manure composting facility were 84.3 (${\pm}15.2$) ppm for spring, 115.2 (${\pm}34.7$) ppm for summer, 76.2 (${\pm}18.9$) ppm for autumn, and 38.1 (${\pm}10.4$) ppm for winter, respectively. Based on the results obtained from this study, the seasonal levels of ammonia in livestock manure composting facility were highest in summer followed by autumn, spring and winterroom (p<0.05). Mean concentrations of hydrogen sulfide in livestock manure composting facility were 7.46 (${\pm}2.24$) ppm for spring, 9.42 (${\pm}2.82$) ppm for summer, 8.15 (${\pm}3.06$) ppm for autumn, and 10.18 (${\pm}4.11$) ppm for winter, respectively. The livestock manure composting facility showed the highest levels of hydrogen sulfide in winter followed by summer, autumn and spring. However, there was no significant difference of hydrogen sulfide concentration among seasons (p>0.05).

Development and Comparative Analysis of Mapping Quality Prediction Technology Using Orientation Parameters Processed in UAV Software (무인기 소프트웨어에서 처리된 표정요소를 이용한 도화품질 예측기술 개발 및 비교분석)

  • Lim, Pyung-Chae;Son, Jonghwan;Kim, Taejung
    • Korean Journal of Remote Sensing
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    • v.35 no.6_1
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    • pp.895-905
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    • 2019
  • Commercial Unmanned Aerial Vehicle (UAV) image processing software products currently used in the industry provides camera calibration information and block bundle adjustment accuracy. However, they provide mapping accuracy achievable out of input UAV images. In this paper, the quality of mapping is calculated by using orientation parameters from UAV image processing software. We apply the orientation parameters to the digital photogrammetric workstation (DPW) for verifying the reliability of the mapping quality calculated. The quality of mapping accuracy was defined as three types of accuracy: Y-parallax, relative model and absolute model accuracy. The Y-parallax is an accuracy capable of determining stereo viewing between stereo pairs. The Relative model accuracy is the relative bundle adjustment accuracy between stereo pairs on the model coordinates system. The absolute model accuracy is the bundle adjustment accuracy on the absolute coordinate system. For the experimental data, we used 723 images of GSD 5 cm obtained from the rotary wing UAV over an urban area and analyzed the accuracy of mapping quality. The quality of the relative model accuracy predicted by the proposed technique and the maximum error observed from the DPW showed precise results with less than 0.11 m. Similarly, the maximum error of the absolute model accuracy predicted by the proposed technique was less than 0.16 m.