• Analytical Science and Technology
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• v.6 no.3
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• pp.313-318
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• 1993

The rose petal tissue biosensor has been constructed by immobilizing New carina rose tissue. Optimum conditions for the determination of urea were investigated using this sensor. Selectivity and life time of this sensor were also obtained. As a result, the biosensor showed the optimum response characteristics in 0.20M phosphate buffer solution at pH 8.0, $37^{\circ}C$ and 50mg of tissue amounts. This sensor was linear from $9.0{\times}10^{-5}$ to $4.0{\times}10^{-3}M$ urea with a slope of 42mV/decade. The limit of detection and response time are $7.0{\times}10^{-5}M$ and 17~19 min.