• Korean Journal of Plant Tissue Culture
• /
• v.26 no.4
• /
• pp.271-274
• /
• 1999

This study was undertaken to develop an efficient propagation technique for mature Betula davurica. Using aseptic materials taken from in vitro culture, the effects of media and plant growth regulators on shoot proliferation and rooting were investigated. DKW medium turned out to be the best in shoot proliferation among the media tested. Whereas axillary buds were better culture material than apical buds in proliferation of shoots, apical buds were slightly better than axillary buds on shoot elongation. Neither 1 /2 MS nor WPM medium seemed to be suitable for shoot multiplication or elongation. When the explants were cultured on 1/2 MS medium, shoot elongation was retarded by forming big callus at the base. In the case of WPM, shoots could be formed normally, but they exhibited slow growing. NAA was so effective on in vitro rooting that more than 80% rooting could be achieved on half-strength DKW medium supplemented with 1.0 mg/L NAA after 4 weeks in cultures. Ex vitro rooting using elongated shoot was also applicable to rooting and acclimatization. Rooted plantlets were successfully acclimatized in an artificial soil mixture and grew normally. The results demonstrate that efficient mass propagation of mature B. davurica can be done through tissue culture.

• Horticultural Science & Technology
• /
• v.18 no.6
• /
• pp.823-826
• /
• 2000

This study was carried out to investigate the effect of plant growth retardants (daminozide, chlormequat, hexaconazole, uniconazole, paclobutrazole) and fungicides of triazole chemical (microbutanil, difenoconazole, terbuconazole, bitertanol) on the rooting of 'New Guinea' impatiens cuttings. In both 'Papete' and 'Anguilla', the formation of adventitious roots was promoted by all concentrations of chlormequat, hexaconazole, uniconazole, and paclobutrazole treated. More adventitious roots were formed with hexaconazole, uniconazole, and paclobutrazole than with chlormequat. Length of the longest root and rooting zone decreased at higher concentrations of chlormequat, hexaconazole, uniconazole, and paclobutrazole in 'Papete', while 'Anguilla' showed less responses. In contrast with plant growth retardants, the formation of adventitious roots was remarkably promoted by fungicides of triazole chemical such as microbutanil, difenoconazole, terbuconazole, and bitertanol in 'Pepete' and 'Martinique' impatiens, but was not in 'Pagopago'. However, length of the longest root and rooting zone showed more sensitive response in 'Martinique' and 'Pagopago' than in 'Pepete'. These results suggest that fungicides of triazole chemical can be effectively used for rooting promotion of impatiens cuttings.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2001.11b
• /
• pp.35-42
• /
• 2001

In order to obtain basic information for pure line breeding of hemp which is effective on the pure line preservation and propagation, three varieties were investigative from 1995 to 1996 at Mokpo Experiment Station, Honam Agriculture Experiment Station, and cutting parts, cutting times, cutting length and storage duration after cutting of plant were observed. Higher rooting ratio were observed in cutting of shoot and later branch, in July 5, optimum harvesting time and 5 days after harvesting time, in shorter storage duration,0 and 2 days. Also there were the highest rooting ratio increased by from 12% to 16% in shorter cutting of 10cm than 15cm long cutting in shoot, lateral branch and stem cuttings. Hemp cv. Korea showed higher rooting ratio than the other varieties, Japan and Turkey. Therefore, it was concluded that hemp, a dioecious plant, could be effectively propagated for the pure lines by cutting method, especially in cv. Korea.

• FLOWER RESEARCH JOURNAL
• /
• v.16 no.1
• /
• pp.71-75
• /
• 2008

The object of this study was to determine the optimum concentrations of boron and IBA and dipping time, and to investigate the effect of combined-treatment of boron and IBA for the improvement of rooting of semi-hardwood cutting of Styrax japonica. The rooting percentage and the number of roots of cuttings increased with short dipping treatment of $7,000mg{\cdot}L^{-1}$ IBA and combined treatment of $100mg{\cdot}L^{-1}$ IBA and $20mg{\cdot}L^{-1}$ boron or $100mg{\cdot}L^{-1}$ IBA and $60mg{\cdot}L^{-1}$ boron. The rooting percentage, no. of roots and fresh weight were higher in combined treatment of boron and IBA than treatment of IBA alone. Either $100mg{\cdot}L^{-1}$ or $200mg{\cdot}L^{-1}$ was regarded as in appropriate level of IBA and the optimal dipping time was three hour in single treatment of IBA. It is concluded that the combined treatment of low concentrations of IBA and boron could be substituted for the treatment of high concentration IBA.

• Journal of Korean Society of Forest Science
• /
• v.70 no.1
• /
• pp.1-6
• /
• 1985

The purpose of this experiment lies in finding the most appropriate cutting condition of Taxus cuspidata Sieb. et Zucc. which has been considered valuable tree species in gardening. Statistical analysis was focused on the comparison of the average ratios of rooting between greenhouses and fields, based on the observation rooted autogenis. To conduct this survey, hardwood cutting of Taxus cuspidata Sieb. et Zucc. were performed at both places of experiment above on April 20, 1982 with rooting results calculated on October 31, 1982. After formation of cuttings, it was soaked in IBA 200 ppm for 12 hours and than put in a bed. A summary of the result is presented as follows; 1) Cutting at the greenhouse showed higher average rate of rooting than at fields. Same tendency was found regardless of the differences in treatment. 2) It was clear that IBA-treatment contributed remarkably to causing higher average rooting rate. As an evidence, when twenty centimeter cuttings were inserted sandy media, IBA-treated area resulted in eighty six percent of average rooting rate, as compared with only twenty three percent in nontreatment case. In case of field cutting, IBA-treatment brought about fifty three percent of rooting in comparison with eleven percent nontreatment. 3) When sandy soil, loam and brown soil were separately used as cutting media, the highest rooting rate was found in case of sandy soil, without any difference between the two experimental places above. 4) As a result of the analysis to seek the impact of the length of cuttings on rooting, the range of length form fifteen to twenty centimeter was apparently most appropriate. It was also found that the rate of rooting declined beyond twenty five centimeter. 5) Two kinds of rooting pattern were observed. One was the case that callus cell lump was created on the lower cut side of cuttings. Importantly, root radical were formed inside the lump to influence the germination of root system. The other relates to the case that adventitious root which look like lateral roots appeared at the stem region. In abstract, first, sandy soil was effectively recommended in case of hardwood cutting in April. Second, the most appropriate length of cuttings ranged between fifteen and twenty centimeters. Third, high density IBA treatment was clearly effective. Forth, for proper environmental management, both pre-disinfection of sail by sterilizer and maintenance of high relative humidity were essentially required.

• Journal of Korean Society of Forest Science
• /
• v.38 no.1
• /
• pp.19-26
• /
• 1978

In order to investigate the difference of rootability between 15 clones of Populus alba${\times}$glandulosa selected based on the growth performance, rooting of cutting experiments with these 15 clones were conducted at the nursery for six years from 1970 to 1975. Cutting experiments in a temperature controlled incubator in which the temperature of the cutting bed were set to $10^{\circ}C$, $15^{\circ}C$, $20^{\circ}C$, $25^{\circ}C$ and $30^{\circ}C$ were also performed. Along with these experiments air layering experiments were performed to compare with the rootabilities obtained from nursery trial. The results obtained so far could be summarized as follows. 1. The best rooting clones were 65-22-4 and 65-22-11, and the average rooting percentages of these two clones for six years were 76.7%, and 72.9% respectively. The poorest rooting clone was 66-6-8 showing average rooting percentage of 45.8%. 2. The middle class of rooting percentage was ocuppied by the clones; 66-14-29, 66-14-93, 66-25-5 and 67-6-3, and the range of their rooting percentage was 60~69% on average. 3. The rooting performances observed through the nursery, the incubator and the air layering experiments were almost the same with exception of few clones. 4. P. alba${\times}$glandulosa showed the best rooting percentage at the cutting bed of $20^{\circ}C$ 5. The most roots, i.e. 78.5% of root per cutting were developed from the bottom part of the cutting shoot. 6. Adventitious and call use roots could observe in the cuttings.

• Korean Journal of Plant Resources
• /
• v.25 no.6
• /
• pp.739-744
• /
• 2012

The plant Empetrum nigrum, valued in the traditional system of medicine, is well known for its antibacterial, antifungal, and antioxidant properties. In the present work, the effect of removal of shoot apical meristem (SAM) on shoot proliferation was studied. It was observed that removal of SAM promoted shoot proliferation whereas intact tip resulted in higher survival percentage. Further, the effect of different concentrations of BA on above was also studied. During root formation the effect of light quality after treatment with IBA was investigated. For rooting, continuous red light without IBA resulted in maximum rooting percentage. The above factors when taken into consideration during micropropagation of this endangered plant can result in healthier plantlets. The results show that the species could be successfully conserved by in vitro propagation system.

• Journal of Plant Biotechnology
• /
• v.30 no.2
• /
• pp.185-188
• /
• 2003

In order to enhance shoot elongation and rooting of Anthurium andreanum 'Atlanta' in vitro, 15mL of liquid media containing various concentrations of activated charcoal, sucrose and MS salts were added in same vessels after small shoots were induced from the calli on mudium supplemented with 10.0mg/L BA and 0.1mg/L 2.4-D. The post-supplying of 15mL liquid medium containing MS macro and micro elements, 30g/L sucrose and 5.0∼10.0g/L activated charcoal was significantly stimulated the shoot elongation and rooting of regenerated shoots from calli. The medium addition was also resulted in the enhanced soil survival, elongation and rooting of plantlets in cultural soil mixed with perlite and vermiculite(1 : 1)

• Journal of Korean Society of Forest Science
• /
• v.82 no.1
• /
• pp.26-33
• /
• 1993

In vitro shoot proliferation and rooting were tested for 2-0 seedlings of half-sib families of 4 plus oaks trees. Nodal segments having axillary buds from 37 families(16 of Quercus acutissima, 10 of Q. variabilis, 7 of Q. serrata, and 4 of Q. mongolica) were cultured on WPM(Woody Plant Medium) supplemented with 0.5 mg/l BA (6-benzyladenine) and 0.01 mg/l NAA(${\alpha}$-naphthalene acetic acid) and subcultured at 2-3 weeks of intervals fur 6 months. In vitro rooting was carried out on GD(Gresshoff and Doy) medium supplemented with 0.5mg/l IBA(indole butyric acid). The capacity for shoot proliferation and rooting was highly varied with families. Generally, white oaks(Q. serrata and Q. mongolica) showed poor response than black oaks(Q. acutissima and Q, variabilis) in shoot proliferation and rooting. Among the total of 37 families, 7 of Q. acutissima, each 2 of Q. variabilis, Q. serrata, and Q. mongolica revealed abilities for continuous shoot proliferation, and the others failed to proliferate. Rooting of the selected oak trees also greatly varied among the families. In Q. acutissima, rooting ratio ranged from 10.0%(CB 25. KG 4) to 89.8%(CB 18). Although 26.7% of KG 16 in Q. variabilis, 3.3% of JN 15 in Q. serrata were rooted, Q. mongolica was not rooted at all in this experimental conditions. No relationship between shoot growth and the rooting ability was observed. Present results suggest the possibility of large-scale micropropagation, but further studies on family differences, shoot-tip necrosis, and callusing of rooting junction are still required to develop reliable micropropagation systems.

• Korean Journal of Environmental Agriculture
• /
• v.31 no.4
• /
• pp.313-317
• /
• 2012

BACKGROUND: Citrus is usually propagated by grafting onto a rootstock. In Korea, As trifoliate orange (Poncirus trifoliata) has dwarf and strong cold hardness, it is widely used as the rootstock of satsuma mandarin. Because 'Shiranuhi' ((Citrus unshiu ${\times}$ C. sinensis) ${\times}$ C. reticulata), a kind of citrus, also, generally is grafted onto a trifoliate orange, most of farmer has been recognized that 'Shiranuhi' root is naturally trifoliate orange. Meanwhile, reduction of flowering in 'Shiranuhi' orchard has been issued among the farmers and researchers over past few years and they guessed it was occurred by severe prune, oversupply of fertilization, overfruiting and temperature of growth period. However, a few researchers strongly assumed that it would be caused by scion rooting of 'Shiranuhi'. So, this study was carried out to identify the existence of scion rooting in 'Shiranuhi' tree in Korea. METHODS AND RESULTS: To identify the existence of scion rooting in 'Shiranuhi' tree, we randomly selected six 'Shiranuhi'orchards and we surveyed three to four trees, which flowering was not enough, from six 'Shiranuhi' orchards respectively. We took the root samples of 'Shiranuhi' mandarin, and then separated the two group which were non-scion rooting (Trifoliate orange), and scion rooting ('Shiranuhi' mandarin). To identity the scion rooting, we used primer set of three types which were a F2/R15, F4/R15 and F5/R15 primer set. As a result, when we conducted the DNA analysis, fourteen tree in less bloomed twenty tree was proved as tree with the scion rooting of 'Shiranuhi' mandarin. CONCLUSION(S): Scion roots of 'Shiranuhi'mandarin were usually observed in a deeply planted tree, and xylem of 'Shiranuhi' root indicated more white color than a case of trifoliata orange. 'Shiranuhi' tree by scion rooting was more vigorous but less flowering than trees grafted onto trifoliata orange. When we used F2/R15, F4/R15 and F5/R15 primer set for discriminance of 'Shiranuhi'mandarin root and trifoliate root, we identified the existence of scion rooting in 'Shiranuhi', From our results, it is suggested that the influence of scion root should be reviewed in 'Shiranuhi'orchards.