• Title/Summary/Keyword: Root growth form

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Rapid micropropagation of wild garlic (Allium victorialis var. platyphyllum) by the scooping method

  • Jeong, Mi Jin;Yong, Seong Hyeon;Kim, Do Hyeon;Park, Kwan Been;Kim, Hak Gon;Choi, Pil Son;Choi, Myung Suk
    • Journal of Plant Biotechnology
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    • v.49 no.3
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    • pp.213-221
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    • 2022
  • Wild garlic (Allium victorialis var. platyphyllum, AVVP) is a nontimber forest product used as an edible and medicinal vegetable. AVVP is usually propagated form offspring bulbs but it takes a long time to harvest. Using tissue culture technology could overcome this problem. This study investigated the optimal conditions for shoot multiplication, root growth, and plant growth by scooping AVVP bulbs. AVVP bulbs harvested from Ulleung Island, Korea, the main producer of AVVP, were surface-sterilized and used for in vitro propagation. Shoot multiplication was performed by the scooping method. More than five multiple shoots were induced from scooped tissue in Quoirin and Lepoivre (QL) medium containing plant growth regulators (PGRs); the maximum number of multiple shoots were induced from scooped tissue in QL medium containing 0.45 μM thidiazuron (TDZ) after 16 weeks of culture. Roots were induced directly at the base of the shoots in all treatments. In vitro rooting depended on the type of PGRs, and the best root-inducing treatment was QL medium containing 9.84 μM indole-3-butyric acid (IBA). Plants with in vitro roots were transferred to pots containing artificial soil and successfully acclimatized for 4 weeks. The acclimatized plants showed a survival rate of 80% after 20 weeks and gradually promoted growth depending on the acclimatization period. The results of this study will be of great help to AVVP dissemination through sustainable mass propagation.

Effects of Nitrogen Form of Nutrient Solution on the Growth of Aster tataricus, Chrysanthemum boreale, and Farfugium japonicum (개미취, 감국 및 털머위 생육에 미치는 양액내 질소원의 영향)

  • Cho, Yeon-Hee;Park, Eun-Ah;Chiang, Mae-Hee
    • Horticultural Science & Technology
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    • v.18 no.1
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    • pp.14-17
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    • 2000
  • This experiment was carried out to investigate the effect of nitrogen form in the Hoagland nutrient solution on the growth of Farfugium japonicum, Aster tataricus and Chrysanthemum boreale. The nitrogen form of Hoagland nutrient solution was modified to $NH_4{^+}(100%),\;NO_3{^-}(100%),\;and\;NH_4{^+}+NO_3{^-}(50%+5%)$ for this study. In the treatment of $NO_3{^-}\;and\;NH_4{^+}+NO_3{^-}(1:1)$, F. japonicum showed the best growth, especially in fresh weight. With the treatment of $NO_3{^-}$, shoot and root fresh weight of A. tataricus and C. boreale were increased. The activity of nitrate reductase and the concentration of chlorophyll and nitrate were increased with $NO_3{^-}$ treatment in all these plants.

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Changes of Reserve Substances in the Bark of Stem and Root Mulberry (Morus alba L.) Graftages after Planting (뽕나무 묘목 식재후 지조 및 뿌리피부의 저장물질의 변화)

  • 성규병;유근섭
    • Journal of Sericultural and Entomological Science
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    • v.32 no.2
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    • pp.79-84
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    • 1990
  • To make clear the changes of the amount of reserve substances in the bark of stem and root of mulberry graftages after planting, studies were carried with mulberry graftages classified into three groups of 7.0-7.9mm, 9.0-9.9mm and 11.0-11.9mm in diameter of stems at 3cm above their base. The results obtained were as follow : 1. The dry weight of stem and root bark gradually decreased with growth of new shoots till about five weeks after planting, and from then it increased. 2. The duration of new shoots growth depending on the reserve substance was limited for 6-7 weeks after planting. In no fertilized group, the length of new shoots increased up to 6-7 weeks, form then decrease. 3. Dry leaf yield three weeks after planting was found to be no difference in the same diameter of saplings between with and without fertilization. 4. The content of chlorophyll in the leaves was higher in thicker group and in fertilized group than thinner and no fertilized one, respectively. 5. There was no difference in the amount of carbohydrates of the bark of stem and root among the stem diameters. There was a transient increase in the amount of total sugar in the bark of stem and root in the first three weeks after planting, followed by gradual decline up to five weeks. The amount of reducing sugar in the bark of stem and root increased both in fertilized and in no fertilized group up to three weeks after planting, and then it decreased. There was a gradual decline in the content of starch in the bark of stem and root both in fertilized and in no fertilized group up to five weeks after planting, followed by gradual increase.

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Effect of Vesicular-Arbuscular Mycorrhizae on the Growth of Bell Pepper and Corn Seedlings (고추와 옥수수 실생의 생장에 미치는 균근의 효과)

  • Mun, Hyeong-Tae;Kim, Chong-Kyun;Choe, Du-Mun
    • The Korean Journal of Ecology
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    • v.13 no.1
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    • pp.1-8
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    • 1990
  • Effects of mycorrhizal infection on the growth of bell pepper (Capsicum annuum) and corn (Zea mays) seedlings have been studied by comparing plants grown in sterilized soil/sand mixtures to plants grown in sterilized soil/sand mixtures with topping the original non-sterile field soil. The original nonsterile field soil, which were taken from the bell pepper field, contained a high level of endmycorrhizal spores. After seven weeks, the shoot height of inoculated plants was increased by 110% in bell pepper, and 90% in corn compared with the control plants. The average above-ground biomass of inoculated plant was increased by 88% in bell pepper and 71% in corn compared with the control plants. The shoot-root ratios in bell pepper and corn were 2.7 and 1.8 for the control plants, and 4.3 and 2.7 for the treatment plants, respectively. Phosphorus level in inoculated plant was higher than that of the control plant. However, nitrogen contents were similar between the control and the treatment plants. The control plants didi not form vesicular-arbuscular mycorrhizae during the experimental period.

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Effects of Water Conditions and Rhizobium Inoculation on the Growth of Wisteria floribunda Seedlings in Slope Soils (절개지 토양에서 수분조건과 근류균 접종이 등나무 유묘생장에 미치는 영향)

  • Park, Chong-Min
    • Korean Journal of Environment and Ecology
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    • v.20 no.4
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    • pp.425-435
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    • 2006
  • The objectives of this research were to investigate the drought resistance as well as the growth of Wisteria floribunda seedlings with the soil water conditions. The seedlings for the research were grown in pots with strict water content control on a frame located outdoors. During the experiments, the soil water contents were adjusted to 5, 10, 20, 30, 40, 50, 60, and 70%. In addition, the effects of Rhizobium inoculation on the growth of seedlings were investigated. The experimental results are summarized as follows: 1. The seedlings in the pots with 5% soil water content withered to death due to the water stress. Withering or any distinct growth was not observed from the seedlings in the 10% soil water content. It can be inferred from these results that about 5% of soil water content is the wilting point of W. floribunda seedlings and about 10% is the critical soil water content of its growth in this experiment soils. Therefore, it seems that W. floribunda possesses a good drought resistance. 2. From the analyses of the main growth parameters such as stem elongation, diameter growth, leaf area growth and total dry weight, it was found that the seedling growth can be improved with increasing soil water contents. The relation between each growth parameter(Y) and the soil water contents(W) was well described by a quadratic equation, $Y=a+bW+cW^2$. 3. In soil water contents higher than 20%, the seedling growth(Y) was remarkable along with-its extended growing period, and related to the growing period(D) by a quadratic equation, $Y=a+bD+cD^2$. 4. The artificial inoculation of Rhizobiun promoted the growth of Wisteria floribunda seedlings. 5. Rhizobium was found to be more readily inoculated and to form more root nodules compared to seedlings grown in higher soil water contents.

In Vitro Mycorrhization and Morphological Characterization of Xanthoconium affine with Pinus densiflora

  • Wang, Eun-Jin;Jang, Yeongseon;Ka, Kang-Hyeon
    • The Korean Journal of Mycology
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    • v.45 no.4
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    • pp.319-327
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    • 2017
  • In this study, we investigated the culture conditions of four ectomycorrhizal fungi, namely, Amanita spissacea NIFoS 2719, Pisolithus arhizus NIFoS 2784, Suillus spraguei NIFoS 2848, and Xanthoconium affine NIFoS 2716, in solid and liquid culture media. In addition, we attempted to induce in vitro mycorrhization of the fungi with Pinus densiflora. Prior to liquid culture, we determined the optimal culture conditions for each species in solid media. The results revealed that all species examined are capable of growth in potato dextrose agar (PDA), malt extract agar (MEA), and modified Melin-Norkran's medium (MMN), although their preferred growth media were different. Liquid culture experiments showed that inorganic nitrogen did not enhance the mycelial growth of all four species. Therefore, we used MMN-based liquid inocula to promote the growth of ectomycorrhizal fungi in our symbiosis culture system. Mycorrhization was observed in Xanthoconium affine NIFoS 2716. Morphological analysis revealed that fungi-inoculated roots of P. densiflora form simple and dichotomous lateral roots with dense mycelia. In addition, inoculation with X. affine NIFoS 2716 promoted root and shoot developments.

Medium Depths and Fixation Dates of 'Seolhyang' Strawberry Runner Plantlets in Nursery Field Influence the Seedling Quality and Early Growth after Transplanting (차근육묘를 위한 배지의 깊이 및 착근 시기가 '설향' 딸기 자묘 소질과 정식 후 초기 생장에 미치는 영향)

  • Park, Gab Soon;Choi, Jong Myung
    • Horticultural Science & Technology
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    • v.33 no.4
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    • pp.518-524
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    • 2015
  • The objective of this research was to investigate the influence of various depths of expanded rice hull (ERH) medium and fixation dates of runner plantlets of 'Seolhyang' strawberry on the growths in nursery field and in plastic house soil after transplanting. The five treatments in medium depths (30, 50, 70, 90, and 110 mm) and four treatments in fixation dates (1st and 15th July and 1st and 15th August) were tested. The growths of runner plantlets were investigated before transplanting to plastic house soil. The early growth and inflorescence rates of crops after transplant to plastic house soil were also investigated. The plant height and fresh weight of runner plantlets were the highest in the medium depths of 50, 70, and 90 mm. The medium depth of 30 mm had higher numbers of first roots, but lower root fresh weight compared to those of 70, 90, and 110 mm. The treatment of 30 mm in medium depth showed poorer growth in all indexes except root length and root weight compared to those of 70, 90, and 110 mm. The runner plantlets fixed on July 1 and July 15 showed good root growth and the weights of ERH adhered to form root balls were 18.3 g and 13.9 g, respectively. The detached amount of ERH was less than 40% in the two treatments when root balls were shaken by a vibratory sieve shaker. The plant growth at 45 days after transplanting to plastic house soil were not significantly different when the runner plantlets were fixed in the period from July 1 to Aug. 1. The inflorescence rates of the first cluster were 93 to 100% when runner plantlets were fixed in the period from July 1 to Aug. 1. By contrast the runner plantlets fixed on the Aug. 15 had a 67% in florescence rate for the first cluster. These results indicate that optimum depth of ERH medium was 7 cm and the ranges of optimum fixation dates are from July 20 to 25.

Allelopathic Effects of Catsear (Hypochaeris radicata L.) for the Development of Environmentally-friendly Agricultural Materials (친환경농자재 개발을 위한 서양금혼초(Hypochaeris radicata L.)의 알레로패시 효과)

  • Cha, Jin-Woo;Kim, Hyoun-Chol;Kang, Jeong-Hwan;Kim, Tae-Keun;Jung, Dae-Cheon;Song, Sang-Churl;Lee, Hee-Sean;Song, Jin-Young;Song, Chang-Khil
    • Korean Journal of Organic Agriculture
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    • v.22 no.1
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    • pp.129-145
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    • 2014
  • To evaluate the potential of Hypochoeris radicata L. for the development of environment-friendly organic materials, its growth characteristics, allelopathic effects and antifungal activity was investigated. The growth characteristics of H. radicata L. was investigated by measuring comparative number of shoot and flower, and the diameters of clones in mowing areas and non-mowing areas, respectively. As a result, the number of shoot and flower, and diameters of clones of H. radicata L. grown in mowing areas were much higher than those grown in non-mowing areas. Water extracts of H. radicata L. inhibited seed germination, and shoot, root and root hair growth of 14 test plants including Trifolium pratense, Festuca myuros, Bidens bipinnata and finally reduced heir biomass remarkably. The inhibitory effect of the extract was different depending on the kind and the part of tested plants. The extracts showed high antifungal activity against Pythium spp. and Phytophthora However, it showed comparably less antifungal activity against Rhizoctonia solani than Pythium spp. and Phytophthora. In conclusion, cutting H. radicata L. resulted increase of its vegetative and seed propagation and helped it to form large colony. Also it had an effect on growth of microbes and germination and growth of other plants. Therefore H. radicata L. holds the competitive dominant position in plant ecosystem in Jeju Island and it can be used as candidate of environment-friendly organic materials.

The Growth Analysis of Sowing Times in Safflower(Carthamus tinctorius L.) (파종시기별 홍화의 생장해석)

  • Kim, Jae-Cheol;Ryu, Jung-Gi;Kim, San-Young;Park, Kyeng-Seok
    • Korean Journal of Plant Resources
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    • v.21 no.1
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    • pp.47-51
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    • 2008
  • This study was carried out to get the basic data for safflower cultivation according to sowing date by growth analysis for 2 years form 2003 to 2004 at Institute of natural product in Uiseong, G.B.A.R.E.S.. Safflower(Cheongsoo cultivar) was seeded at March 1st, April 1st, May 1st, and July 1st in field. The necessary date for germination was 24 days, when safflower was seeded at March 1st, but it was 8 days which sowed at April 1st and when the seed was sowed at high temperature period, the necessary date for germination was getting short. The necessary date for flowering was the same trend as germination; it took 104 days at March 1st, 79 days at April 1st, 65 days at May 1st, and 58 days at July 1st sowing treatment. The safflower growth was the most vigor when it was sowed at March 1st. T/R(Top/Root) ratio was gently increased during growing season, but when the seed was sowed at July 1 st, it was low compare to sowing at March 1st and April 1st. RGR(Relative growth rate) was the highest during shoot growth stage, but it was rapidly decreased after flowering. CGR(Crop growth rate) was increased at branching stage, and the increasing velocity was slightly decreased just before flowering, and it was increased again at flowering and at this stage, CGR was the highest, and then it did not increased. NAR(Net assimilation rate) was the highest at shoot growth stage during growing season, and was the highest which was sowed at March 1st.

Spillovers of Education and Job Training in Bringing Sustainable Economic Development in Pakistan: The Role of Research and Development Revisited

  • AMIN, Sara;LIAQAT, Malka;SAFDAR, Noreen;IQBAL, Sidra
    • The Journal of Asian Finance, Economics and Business
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    • v.9 no.5
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    • pp.11-18
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    • 2022
  • Current research intended to measure the spillovers of education and on-the-job training to enhance sustainable economic development. Consideration of research and development expenditures for on-the-job training expands the impact of education and, thus, may provide spillovers for Pakistan's economic growth. The primary focus of this research is to look at the combined impact of education and research and development spending as value-added variables for sustainable growth. The econometric study uses data from a variety of sources, including the Pakistan Bureau of Statistics, the Pakistan Economic Survey, and world development indices. The secondary annual time series data from 1976 to 2019 are analyzed. To adequately estimate the empirical model, ARDL has been used while the presence of the unit root has been tested using the ADF test. The findings show that education, research, and development all contribute to all-inclusive, sustainable economic growth. Labor and capital, among other traditional components, continue to be the backbone of the development process. Still, the spillover effect is much enhanced with the expansion in education and the on-job training in the form of research and development expenditures. Consequently, the introduction of research and development in education will lead to a sustainable and inclusive economy.