• Title/Summary/Keyword: Root Rot

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Biological activities of the diethyl ether soluble toxin produced by Helminthosporium sativum (Helminthosporium sativum이 생성하는 D-toxin의 생물학적 활성)

  • Lee, Sang-Sun;Vick, Brady A.;Stack, Robert W.
    • The Korean Journal of Mycology
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    • v.19 no.2
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    • pp.128-135
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    • 1991
  • Diethyl ether soluble toxin produced by H. sativum had the characteristics of helmin­thosporal as based on UV, GC-MS, and chemical analysis, but was not a helminthosporaI. It was speculated that it was a polymer of helminthosporal. It stimulated the productions of reducing sugar in the barly endosperm like gibberellic acid, but acted in the responses on the barley roots and coleoptiles like gibberellic acid. It seemed to be involved in Foliar and Root rot diseases with host specificity, based on the analysis of linear regression.

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Effect of Metalaxyl on Controlling Phytophthoyra Disease of Korea Ginseng (인삼역병에 대한 Metalalryl의 방제효과)

  • 유연현;오승환
    • Journal of Ginseng Research
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    • v.9 no.2
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    • pp.163-169
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    • 1985
  • The efficacy of fungicides was compared for control of root rot as well as leaf blight caused by Phytophthora cactorum on ginseng plants. Growth of P. cactorum in rlitro was completely or highly inhibited by metalaxyl, tetracyclin, captafol, carbendazim, and thiophanate + thiram. In field trials, the disease was significantly reduced not only in the root rot but also in the leaf blight when metalaxyl was applied at 4.17 mg a.i. per plant for soil drenching and 1.25 mg a.i. for foliage application. Also captafol was effective on control of the leaf blight but its effect was inferior to that of metalaxyl. Metalaxyl lost its effectiveness in vivo between the 5th and 7th week after soil wren ching. Phytotoxicity was, however, observed on 2 years old ginseng plants when metalaxyl was drenched at 8 mg a.i. while no phytotoxic symptom was developed on 2 years old ginseng plants at 4k mg a.i. and 3 years old at 16 mg a.i. per plant, respectively.

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Chlamydospore Induction from Conidia of Cylindrocarpon destructans Isolated from Ginseng in Korea

  • Kang, Yunhee;Kim, Mi Ran;Kim, Ki Hong;Lee, Jungkwan;Lee, Seung-Ho
    • Mycobiology
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    • v.44 no.1
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    • pp.63-65
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    • 2016
  • Cylindrocarpon destructans causes root rot disease in ginseng and can survive for a long time, producing chlamydospores. We optimized conditions to induce chlamydospore production from the conidia of C. destructans, isolated from Korean ginseng. This will provide the basis for testing the efficacy of control agents targeting these chlamydospores.

Resistance to Northern Root-knot Nematode, Meloidogyne hapla, in Peony Strains (당근뿌리혹선충에 대한 작약계통의 저항성 검정)

  • 박소득;김재철
    • The Korean Journal of Soil Zoology
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    • v.6 no.1_2
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    • pp.33-36
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    • 2001
  • This study was carried out to screen 11 peony varieties collected for resistance to northern root-knot nematode, Meloidogyne hapla. The larval population of Meloidogyne halpa to the Uisongjakyak, Taebaekjakyak, Doseo, EP368, EP512 peony were detected high denisties with individuals of 98-173 per 300 ml soil, but Sabeol, Yongsu, Kimchon, Pl23 peony showed low number of larvar with 21-60. The root rot were lowered in Yongsu, Kimchon peony than Uisongjakyak, Taebaekjakyak, EP368, EP512. It seems to involved with densities of 2nd juvernile population in that rot rate of root was increased as increase of 2nd stage larvae. Two peony, Uisongjakyak, Taebaekjakyak were susceptable to M. hapla with high egg mass formation which showed 49,28 in each root. And Yongsu, Doseo, EP368, EP512 were Mid resistance with 1-15 egg mass of each root. Whereas Sabeol, Kimchon, Pl23, EP337, EP425 were found to be resistant to M, hapla with few egg mass formation of 4-7 each root.

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Antifungal Activity or Coptis japonica Root-stem extract and Identification of Antifungal Substances (황련추출액의 항균활성과 항균성물질의 동정)

    • Korean Journal of Plant Resources
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    • v.12 no.4
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    • pp.260-268
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    • 1999
  • Crude extract of Coptis japonica root-stem was evaluated for antifungal activity against Phytophthora capsici, Fusarium oxysporum, Colletotrichum dematium, Colletotrichum truncatum, Botrytis cinerea, Botryosphaeria dothidea and Alternaria porri, and antifungal active compound from the extract was identified. In addition, the usefulness of the extract for some plants disease control was investigated. Crude extract of C. japonica root-stem exhibited antifungal activity against P. capsici, F. oxysporum, C. dematium, B. cinerea, B. dothidea and A. porri. Antifungal activity of the substance isolated from C. japonica root-stem was similar to a standard chemical berberine-Cl. Red-pepper fruit rot, sesame stem rot and welsh-onion alternaria leaf spot were effectively controlled by the crude extract of C. japonica root-stem. Phytotoxicity was not observed in the red-pepper and welsh-onion leaves and red-pepper and strawberry fruits with exogenous foliage application of the crude extract. Seeds germination and radicle growth of red-pepper and sesame were inhibited by the crude extract of C. japonica root-stem. 4.24g of yellowish compound per 100g of C. japonica root-stem was obtained. The compound was identified as berberine-Cl by HPLC.

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The Outbreak and Propagule formation of black root rot caused by Calonectria crotalariae in Korea (콩 흑색뿌리썩음병의 발생과 Propagule의 형성)

  • Sung J.M.;Park J.H.;Lee S.C.;Chung B.K.
    • Korean journal of applied entomology
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    • v.19 no.4 s.45
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    • pp.228-233
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    • 1980
  • The infection rate of soybean black root rot disease caused by Calonectria crotalariae was about $14\%$. The isolated fungi from the infected soybean roots and stems were Calonectria crotalariae, Fusarium solani, F. roseum, Phomopsis sojae, Pythium aphanidermatum, Rhizoctonia solani and Macrophomina sp. Among them, C. crotalariae was the most virulent pathogen under the laboratory conditions. Mycelial growth and microsclerotial formation were good on PSA containing 1000cc of water, 100g of potato and 20g of sugar. Mycelial growth, sporulation and microsclerotial formation were good on sterilized root. Perithecial formation was better in the dark condition than in the light. Survival of macroconidia was not available between $0\~25\%$ soil water content. Microsclerotia and mycelium in infected plant debris were survived for 4 months at to $8\%\;50\%$ soil water content. The plant height, when inoculated with $1.2\%$ inoculum density, reached approximately half of uninoculated plants. Disease severity was much higher at nonsterilized soil than completely sterilized soil. It was determined that the host range of this pathogen includes soybean, peanut, green bean and red bean.

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Measurement of Dilution End-Points and Phytotoxicity of Toxic Metabolites Produced by Helminthosporium sativum in Barley, Wheat and Lettuce Roots (Helminthosporium sativum가 생성하는 독소물질에 대한 phytotoxicity 및 Dilution end-Points 측정 방법 개발)

  • Lee Sang. S.
    • Korean Journal Plant Pathology
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    • v.3 no.3
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    • pp.198-202
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    • 1987
  • Toxic metabolites ('Toxins'), produced by Helminthosporium sativum causing leaf blotch in barley and root rot in barley and wheat were partially purified through C-18 column. The partially purified toxins appeared heat unstable and lipophilic. The responses of toxins to wheat and barley root corresponded with those to lettuce growth with the different concentrations. The determination of the concentration of toxins produced was developed using the dilution end-points. The equation [Y = a log X + b) was obtained from the semi-log­graphy with the linear analysis. The values 'a' and 'b' were discussed with the responses of several plants on the toxin produced by H. sativum.

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Effects of Prochloraz and Tebuconazole on Control of Fusarium Bulb and Root Rot of Oriental Orchid, Cymbidium goeringii (Prochloraz와 Tebuconazole의 Fusarium oxysporum에 의한 춘란(Cymbidium goeringii) 구경썩음병 방제효과)

  • Jee, Hyeong-Jin;Lee, Sun-Mi;Cho, Weon-Dae
    • Research in Plant Disease
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    • v.9 no.2
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    • pp.72-78
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    • 2003
  • Eight fungicides including prochloraz, tebuconazole, benomyl, mancozeb, thiophanate-methyl, azoxystrobin, and fluazinam were examined for their control effects on Fusarium bulb and root rot of oriental orchid, Cymbidium goeringii. Among the chemicals, prochloraz and tebuconazole were the most effective on suppression of the causal pathogen, F. oxysporum in vitro and on control of the disease in vivo. Prochloraz and tebuconazole inhibited mycelial growth of the fungi 95~100% at 10ppm a.i. and microconidial germination 75~100% at 100ppm a.i. Prochloraz and tebuconazole showed 80~92% and 84~88% protective control value on the disease, respectively. However, curative effects on infected orchid were relatively low. Other chemicals showed no or lower than 20% curative and 50% protective control value. Results indicated that prochloraz and tebuconazole can be used for the control of the Fusarium bulb and root rot of oriental orchids. However, the chemicals need to be applied prior to the disease development to achieve successful control efficacy.

In vitro Inhibition of Fungal Root-Rot Pathogens of Panax notoginseng by Rhizobacteria

  • Guo, Rongjun;Liu, Xingzhong;Li, Shidong;Miao, Zuoqing
    • The Plant Pathology Journal
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    • v.25 no.1
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    • pp.70-76
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    • 2009
  • The rhizobacteria of Panax notoginseng were isolated from six sites in Yanshan, Maguan and Wenshan Counties, Yunnan Province of China, and their antagonistic activity against P. notoginseng root-rot fungal pathogens was determined. Of the 574 rhizobacteria isolated, 5.8% isolates were antagonistic in vitro to at least one of the five pathogens, Cylindrocarpon didynum, Fusarium solani, Phytophthora cactorum, Phoma herbarum, and Rhizoctonia solani. The number of rhizo bacteria and the number that inhibited fungi differed depending on sampling sites and isolation methods. Rhizobacteria isolated from the site in Yanshan and Maguan showed more antagonistic effect than them in Wenshan. Heat treatment of rhizosphere soil at $80^{\circ}C$ for 20 min scaled the antagonists up to 14.0%. Antagonistic bacteria in the roots proportioned 3.9% of the total isolates. The most antagonistic isolates 79-9 and 81-4 are Bacillus subtilis based on their 168 rDNA sequence and biochemical and physiological characteristics. Identification and evaluation of antagonistic bacteria against P. notoginseng root-rot pathogens in the main planting areas improved our understanding of their distribution in rhizosphere soil. Furthermore these results indicated that the interactions between biocontrol agent and soil microbes should be seriously considered for the successful survival and biocontrol efficacy of the agents in soil.