• BMB Reports
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• v.56 no.9
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• pp.508-513
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• 2023

The phytochemical quercetin has gained attention for its anti-inflammatory and anti-tumorigenic properties in various types of cancer. Tumorigenesis involves the aberrant regulation of kinase/phosphatase, highlighting the importance of maintaining homeostasis. Dual Specificity Phosphatase (DUSP) plays a crucial role in controlling the phosphorylation of ERK. The current study aimed to clone the DUSP5 promoter, and investigate its transcriptional activity in the presence of quercetin. The results revealed that quercetin-induced DUSP5 expression is associated with the serum response factor (SRF) binding site located in the DUSP5 promoter. The deletion of this site abolished the luciferase activity induced by quercetin, indicating its vital role in quercetin-induced DUSP5 expression. SRF protein is a transcription factor that potentially contributes to quercetin-induced DUSP5 expression at the transcriptional level. Additionally, quercetin enhanced SRF binding activity without changing its expression. These findings provide evidence of how quercetin affects anti-cancer activity in colorectal tumorigenesis by inducing SRF transcription factor activity, thereby increasing DUSP5 expression at the transcriptional level. This study highlights the importance of investigating the molecular mechanisms underlying the anti-cancer properties of quercetin, and suggests its potential use in cancer therapy.

• Journal of Korean Academy of Nursing
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• v.32 no.3
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• pp.373-383
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• 2002

This study was performed to develope a scale of gender role identity in Korean adults based on the Bem's theory of androgyny. Although there were several tools in Korea, they were revealed having some problems of cultural differences, translation biases, and methodological problems. Methods: A list of 78 items were developed using the existing tools and descriptions from 5 married couples. The items were the typical personality characteristics which were manifested by gender, male or female. And the list contained several items which were the socially desirable personality characteristics. which would be simply used as contextual items. Validity of the 78 items were screened by 18 expert panels with 4 point Likert scale, and 57 items were judged as highly valid from 70% of the experts, which were selected as preliminary items for the tool. Using the preliminary tool which was developed as a 4 point Likert scale, data were collected from 1,127 subjects for item analysis and factor analysis. 53 items were remained, because 4 items whose item-total correlation were lower than 0.2 were excluded by the result of item analysis. Factor analysis was done with the 53 items, and 49 items whose factor loadings were same and higher than 0.4 were remained. 3 factors were identified with eigen value 2.0, and these factors were named as masculinity, femininity, and social desirability. Results and Conclusion: KGRII(Korean Gender Role Identity Inventory) which contained 45 items was developed, with 15 items for 3 factors. The reliability of the tool was very high. Cronbach alpha of the tool was 0.929, and alpha of the subscales were ranged from 0.841 to 0.922.

• The Journal of the Korean bone and joint tumor society
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• v.20 no.1
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• pp.1-6
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• 2014

Purpose: To examine the expression of Connective Tissue Growth Factor (CTGF) in osteosarcoma and to evaluate its role in osteosarcoma invasion and proliferation. Materials and Methods: The mRNA expression of CTGF from 23 patient-derived osteosarcoma cell lines was examined, and the role of CTGF in cell invasion and proliferation was examined using siRNA transfection. Results: The over-expression of CTGF mRNA was observed in 17 cell lines (74%). CTGF-specific siRNA transfection into SaOS-2 and MG63 cell lines resulted in efficient knockdown of CTGF expression on Western blot analysis. siRNA transfected cells showed decreased migration on Matrigel invasion assay and decreased cell proliferation on WST-1 assay. Conclusion: These results indicated that the CTGF expression may play an important role in osteosarcoma progression, and may be a therapeutic target of osteosarcoma.

• Asian-Australasian Journal of Animal Sciences
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• v.29 no.8
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• pp.1065-1074
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• 2016

Growth factors play an important role during early ovarian development and folliculogenesis, since they regulate the migration of germ cells to the gonadal ridge. They also act on follicle recruitment, proliferation/atresia of granulosa cells and theca, steroidogenesis, oocyte maturation, ovulation and luteinization. Among the growth factors, the growth differentiation factor 9 (GDF9) and the bone morphogenetic protein 15 (BMP15), belong to the transforming growth factor beta (TGF-${\beta}$) superfamily, have been implicated as essential for follicular development. The GDF9 and BMP15 participate in the evolution of the primordial follicle to primary follicle and play an important role in the later stages of follicular development and maturation, increasing the steroidogenic acute regulatory protein expression, plasminogen activator and luteinizing hormone receptor (LHR). These factors are also involved in the interconnections between the oocyte and surrounding cumulus cells, where they regulate absorption of amino acids, glycolysis and biosynthesis of cholesterol cumulus cells. Even though the mode of action has not been fully established, in vitro observations indicate that the factors GDF9 and BMP15 stimulate the growth of ovarian follicles and proliferation of cumulus cells through the induction of mitosis in cells and granulosa and theca expression of genes linked to follicular maturation. Thus, seeking greater understanding of the action of these growth factors on the development of oocytes, the role of GDF9 and BMP15 in ovarian function is summarized in this brief review.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.9
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• pp.4137-4142
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• 2015

The zinc finger transcription factor EGR 1 has a role in controlling synaptic plasticity, wound repair, female reproductive capacity, inflammation, growth control, apoptosis and tumor progression. Recent studies mainly focused on its role in growth control and apoptosis, however, little is known about its role in epithelial-mesenchymal transition (EMT). Here, we aim to explore whether EGR 1 is involved in TGF-${\beta}1$-induced EMT in non-smallcell lung cancer cells. Transforming growth factor (TGF)-${\beta}1$ was utilized to induce EMT in this study. Western blotting, RT-PCR, and transwell chambers were used to identify phenotype changes. Western blotting was also used to observe changes of the expression of EGR 1. The lentivirus-mediated EGR 1 vector was used to increase EGR 1 expression. We investigated the change of migration to evaluate the effect of EGR 1 on non-small-cell lung cancer cells migration by transwell chambers. After stimulating with TGF-${\beta}1$, almost all A549 cells and Luca 1 cells (Non-small-cell lung cancer primary cells) changed to mesenchymal phenotype and acquired more migration capabilities. These cells also had lower EGR 1 protein expression. Overexpression of EGR 1 gene with EGR 1 vector could decrease tumor cell migration capabilities significantly after adding TGF-${\beta}1$. These data s howed an important role of EGR 1 in the EMT of non-small-cell lung cancer cells, as well as migration.

• Journal of Ginseng Research
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• v.41 no.3
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• pp.298-306
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• 2017

Background: Compound K (CK) is a bioactive derivative of ginsenoside Rb1 in Panax ginseng (Korean ginseng). Its biological and pharmacological activities have been studied in various disease conditions, although its immunomodulatory role in innate immunity mediated by monocytes/macrophages has been poorly understood. In this study, we aimed to elucidate the regulatory role of CK on cellular events mediated by monocytes and macrophages in innate immune responses. Methods: The immunomodulatory role of CK was explored by various immunoassays including cell-cell adhesion, fibronectin adhesion, cell migration, phagocytic uptake, costimulatory molecules, reactive oxygen species production, luciferase activity, and by the measurement of mRNA levels of proinflammatory genes. Results: Compound K induced cell cluster formation through cell-cell adhesion, cell migration, and phagocytic activity, but it suppressed cell-tissue interactions in U937 and RAW264.7 cells. Compound K also upregulated the surface expression of the cell adhesion molecule cluster of differentiation (CD) 43 (CD43) and costimulatory molecules CD69, CD80, and CD86, but it downregulated the expression of monocyte differentiation marker CD82 in RAW264.7 cells. Moreover, CK induced the release of reactive oxygen species and induced messenger RNA expression of proinflammatory genes, inducible nitric oxide synthase, and tumor necrosis factor-alpha by enhancing the nuclear translocation and transcriptional activities of nuclear factor kappa-B and activator protein-1. Conclusion: Our results suggest that CK has an immunomodulatory role in innate immune responses through regulating various cellular events mediated by monocytes and macrophages.

• 대한약리학회:학술대회논문집
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• 2006.10a
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• pp.112.2-112.2
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• 2006

• Proceedings of the Korean Society of Life Science Conference
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• 2006.09a
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• pp.29.1-29.1
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• 2006

• 대한생식의학회:학술대회논문집
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• 2000.06a
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• pp.9-9
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• 2000

• Journal of Families and Better Life
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• v.29 no.5
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• pp.85-105
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• 2011

The purpose of this study was to investigate the variables on adolescents' perception of parents and children's relationship. Independent variables were the expectation of the parents' role, role performance by adolescents' perception and socio-demographic variables. In order to achieve this purpose, the survey was taken by 758 adolescents (middle & high school students) from Daegu. The data were analyzed by frequency, percentage, mean, Factor Analysis, Cronbach's ${\alpha}$, Pearson's correlation coefficient, Multivariate Analysis of Variance, Scheffe test for post hoc, and Multiple Regression. The major findings were: 1) Role expectation had significant differences in the grade, gender, birth order, father's level of education, father's job, mother's age, and household economy of socio-demographic variables. Role performance showed significant differences in the grade, gender, father's age, parents' level of education, father's job, and household economy of socio-demographic variables. 2) Adolescents' perception of parents and children's relationship was affected by role expectation and role performance.