• BMB Reports
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• 제51권8호
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• pp.388-393
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• 2018

The activating transcription factor (ATF) 4 belongs to the ATF/CREB (cAMP Response Element Binding bZIP [Basic Leucine Zipper]) transcription factor family, and plays a central role in the UPR (Unfolded Protein Response) process in cells. The induction of ATF4 expression has previously been shown to increase the replication of HIV-1. However, the detailed mechanism underlying this effect and the factors involved in the regulation of ATF4 function are still unknown. Here, we demonstrate first that knocking out ATF4 using siRNA shows a strong negative effect on HIV-1 production, indicating that ATF4 is a functional positive cellular factor in HIV-1 production. To determine the mechanism by which ATF4 regulates the HIV-1 life cycle, we assessed the effect of the overexpression of wild type ATF4 and its various derivatives on HIV-1 LTR-mediated transcriptional activation and the production of HIV-1 particles. This effect was studied through co-transfection experiments with either reporter vectors or proviral DNA. We found that the N-terminal domains of ATF4 are involved in HIV-1 LTR-mediated transcriptional activation, and thus in HIV-1 production.

• BMB Reports
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• 제35권4호
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• pp.371-376
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• 2002

The receptor activator of nuclear factor kappa B (RANK) is a member of the tumor necrosis factor (TNF) receptor superfamily. It plays a critical role in osteoclast differentiaion, lymph node organogenesis, and mammary gland development. The stimulation of RANK causes the activation of transcription factors NF-${\kappa}B$ and activator protein 1 (AP1), and the mitogen activated protein kinase (MAPK) c-Jun N-terminal kinase (JNK). In the signal transduction of RANK, the recruitment of the adaptor molecules, TNF receptor-associated factors (TRAFs), is and initial cytoplasmic event. Recently, the association of the MAPK kinase kinase, transforming growth factor-$\beta$-activated kinase 1 (TAK1), with TRAF6 was shown to mediate the IL-1 signaling to NF-${\kappa}B$ and JNK. We investigated whether or not TAK1 plays a role in RANK signaling. A dominant-negative form of TAK1 was discovered to abolish the RANK-induced activation of AP1 and JNK. The AP1 activation by TRAF2, TRAF5, and TRAF6 was also greatly suppressed by the dominant-negative TAK1. the inhibitory effect of the TAK1 mutant on RANK-and TRAF-induced NF-${\kappa}B$ activation was also observed, but less efficiently. Our findings indicate that TAK1 is involved in the MAPK cascade and NF-${\kappa}B$ pathway that is activated by RANK.

• 한국의류산업학회지
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• 제14권3호
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• pp.390-399
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• 2012

The purpose of this study is to find the connectional structure of mentoring perceived by the 2-year college students who are majoring in fashion, and to develop the scale with which the mentoring effect can be measured on the basis of the results. To achieve the purpose, research was conducted through a survey questionnaire. The research was carried out obtaining data from the 2-year college students. Responses from this questionnaire were gathered from 265 students. The questionnaire was made with 31 mentoring scales and removed inadequate 18 questions for statistics analysis. The remaining questionnaires consisting of 13 questions were categorized into three factors. The first factor (5questions) relates to the sensitivity developmental function. The second factor (5questions) pertains to the psychosocial function and the third factor (3questions) was for the fashion career developmental function. The results derived from the analysis of the questionnaire survey are as follows: First, the role model function is not enticing enough for the student to continue role model or professor for future student. The students are more interested into discovering their own capability in fashion by entering a fashion company. Second, the result of this study was confirmed through descriptive statistics, exploratory factor analysis and confirmatory factor analysis. Third, the results of this study can be a guide-line for professors to help student to which is fashion in spite of outside comment and own future career task.

• 대한통합의학회지
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• 제7권4호
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• pp.61-69
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• 2019

Purpose : Human gingival fibroblast cell is one of the the main cell types in periodontal tissue, which they can show anti-inflammatory activity through the production of numerous lines of inflammatory mediators such as inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-2 and interleukins. Porphyromonas gingivalis, one of the oral pathogens, has reported to play a critical role in the development of periodontal diseases. This study aimed to investigate anti-inflammatory and antioxidative activities of Gracilaria textorii ethanol extract (GTEE) in P. gingivalis derived lipopolysaccharide (LPS-PG) stimulated human gingival fibroblast (HGF)-1 cell line. Methods : In order to analyze anti-inflammatory and antioxidative activities of GTEE in HGF-1 cell line, NOS enzyme activity, expression levels of iNOS, COX-2, NAD(P)H quinone dehydrogenase (NQO)1 and their transcription factors were estimated by Griess reaction and western hybridization. Results : LPS-PG induced overexpression of iNOS and COX-2, which was significantly attenuated by GTEE treatment in a dose-dependent manner without any cytotoxicity. In addition, intracellular NOS activity was in accordance with the result of iNOS expression. Due to important role in the regulation of inflammatory responses, phosphorylated status of p65 and c-jun, each subunit of nuclear factor (NF)-κB and activator protein (AP)-1, was also dose-dependently ameliorated by GTEE treatment. One of phase II enzymes, NQO1, and its transcription factor, nuclear factor erythroid 2-related factor 2 (Nrf2), were analyzed since elevated phase II enzyme expression inhibited inflammatory response, which was significantly elevated by GTEE treatment in HGF-1 cell line. Conclusion : In conclusion, GTEE mitigated LPS-PG-stimulated inflammatory responses by attenuating NF-κB and AP-1 activation as well as accelerating NQO1 and Nrf2 expression in HGF-1 cell line. These results indicate that GTEE might be utilized a promising strategy for potential anti-inflammatory agent in periodontal diseases.

• 대한가정학회지
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• 제34권4호
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• pp.86-101
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• 1996

The purpose of the study was to investigate whether there were differences between the Paternal child rearing Involvement and the Parental Satisfaction according to child's sex, father's age and the birth order of child. The subjects surveyed were 271 fathers 132 in their thiries and 139 in their forties who live in Kwang-ju. And the children considered are 128 boys and 143 girls. Among them, first-born children are 143 members, second-born are 103, and third-born are 25. Factor analysis, frequencies, mean, standard deviation, Cronbach's α, one way-ANOVA, Pearson's correlation coefficient, and step-wise regression are used for data-analysis. The main results were as follows : (1) There were some significant differences in the Paternal child rearing Involvement according to the child's sex, while there was no difference as related the father's age and the birth-order of child. (2) The were some significant differences in the father's Parental Satisfaction which is involved child's sex and the father's age, but there was no difference as to the birth-order of child. (3) There were some significant differences between the Paternal Child rearing Involvement and the Parental Satisfaction, and between its subfactor and the Parental Satisfaction, too. (4) The result of the step-wise regression, which analyses the Paternal child rearing Involvement and the background variables as to father's Parental Satisfaction, shows the Parent-child relationship variable (accounted for about32% of the general variation), spouse support, support of children, general satisfaction, and parent's role conflict at intensity in order. Of the above mentioned five fields, house-activities were the first factor in determining this order. And the personal interaction plays an important role in fulfilling general satisfaction and the support of children. The leisure-action factor was the second explanatory factor in establishing the parent-child relationship. Finally father's age was the fourth explanatory factor in assessing the parent-child relationship variable considering the background variables.

• IMMUNE NETWORK
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• 제7권3호
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• pp.117-123
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• 2007

Background: This study was designed to investigate the role of the hepatocyte growth factor (HGF) with regards to differentiation of somatic stem cells originating from the human umbilical cord blood (UCB) into hepatic lineage cells in vitro culture system. Methods: Mononuclear cells from UCB were cultured with and without HGF based on the fibroblast growth factor (FGF)-1, FGF-2, and stem cell factor. The cultured cells were confirmed by immunofluorescent staining analysis with albumin (ALB), cytokeratin-19 (CK-19), and proliferating cell nuclear antigen (PCNA) MoAb. ALB and CK-18 mRNA were also evaluated by reverse transcription-polymerase chain reaction. In order to observe changes in proliferating capacity with respect to the cultured period, CFSE with affinity to proliferating cells were tagged and later underwent flow cytometry. Results: In the HGF-treated group, cultured cells had a large oval shaped appearance with adherent, but easily detachable characteristics. In the HGF-non treated group, these cells were spindle-shaped with strong adherent characteristics. Expressions of ALB and CK-19 were evident in HGF-treated group compared to non-expression of those in to HGF-non treated group. Dual immunostaining analysis of the ALB producing cells showed presence of PCNA in their nuclei, and ALB and CK-18 mRNA were detected on the 21st day of cultured cells in the HGF-treated group. Conclusion: Our findings suggest that HGF has a pivotal role in differentiating somatic stem cells of human UCB into hepatic lineage cells in vitro.

• BMB Reports
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• 제57권4호
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• pp.167-175
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• 2024

Cancer progression is driven by genetic mutations, environmental factors, and intricate interactions within the tumor microenvironment (TME). The TME comprises of diverse cell types, such as cancer cells, immune cells, stromal cells, and neuronal cells. These cells mutually influence each other through various factors, including cytokines, vascular perfusion, and matrix stiffness. In the initial or developmental stage of cancer, neurotrophic factors such as nerve growth factor, brain-derived neurotrophic factor, and glial cell line-derived neurotrophic factor are associated with poor prognosis of various cancers by communicating with cancer cells, immune cells, and peripheral nerves within the TME. Over the past decade, research has been conducted to prevent cancer growth by controlling the activation of neurotrophic factors within tumors, exhibiting a novel attemt in cancer treatment with promising results. More recently, research focusing on controlling cancer growth through regulation of the autonomic nervous system, including the sympathetic and parasympathetic nervous systems, has gained significant attention. Sympathetic signaling predominantly promotes tumor progression, while the role of parasympathetic signaling varies among different cancer types. Neurotransmitters released from these signalings can directly or indirectly affect tumor cells or immune cells within the TME. Additionally, sensory nerve significantly promotes cancer progression. In the advanced stage of cancer, cancer-associated cachexia occurs, characterized by tissue wasting and reduced quality of life. This process involves the pathways via brainstem growth and differentiation factor 15-glial cell line-derived neurotrophic factor receptor alpha-like signaling and hypothalamic proopiomelanocortin neurons. Our review highlights the critical role of neurotrophic factors as well as central nervous system on the progression of cancer, offering promising avenues for targeted therapeutic strategies.

• 치위생과학회지
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• 제16권1호
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• pp.62-69
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• 2016

이번 연구는 치위생(학)과 학생들의 임상실습에 대한 만족도와 임상실습 스트레스 요인을 파악하고, 임상실습만족도와 스트레스 요인들 간의 상관관계를 알아보고자 2012년 5월 21일부터 6월 22일까지 병원 임상실습 경험이 있는 치위생(학)과 학생 232명을 대상으로 설문조사를 하였으며, SPSS WIN 12.0 프로그램을 이용하여 분석한 후 다음과 같은 결과를 얻었다. 스트레스 요인은 학제별로 환경요인, 역할요인, 대인관계요인, 이상과 가치 요인, BEPSI 모든 요인이 4년제보다 3년제에서 높았고(p<0.01), 실습만족도에 따른 스트레스 요인은 불만족일 때 모든 요인이 높았으며, 그 중 환경요인이 가장 높았다(p<0.001). 교육기관에 따라 환경요인은 치과대학병원이 가장 높았고, 대인관계요인과 이상과 가치요인은 치과병원, 역할요인과 BEPSI는 치과의원이 가장 높은 것으로 나타났다(p<0.01). 병원분위기가 나쁠 때 환경요인이 가장 높았고, 역할요인, 대인관계요인, 이상과 가치요인, BEPSI순이었다(p<0.001). 병원실습 스트레스 요인간의 상관관계를 분석한 결과 실습만족도, 환경, 역할, 대인관계, 이상과 가치, BEPSI 간에는 양의 상관관계가 있었고(p<0.01), BEPSI에 가장 큰 영향력을 보이는 스트레스 요인은 대인관계였으며, 환경, 이상과 가치 순으로 나타났다(p<0.001). 임상실습에서 낯선 환경과 대인관계의 부족으로 학생들은 평소보다 스트레스 지수가 더 높을 수 있으므로 학생들이 스트레스에 대해 스스로 잘 대처할 수 있는 능력을 키워주기 위한 체계적인 임상실습 운영방안을 마련해야 할 것으로 생각된다.

• 지역사회간호학회지
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• 제16권3호
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• pp.320-328
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• 2005

Purpose: This study is to identify the types of role awareness of home health care specialists working at medical institutions, to understand their role awareness correctly by grasping the quality of each type, and to provide useful help in the education of home health care specialists. Method: Q-methodology is used to objectify role awareness of medical institution specialists who may recognize situations differently according to their individual experience and comprehension based on the view of behavior. Q-classification was carried out on 30 home health care specialists working at medical institutions using 30 selected questions. Collected data were examined through factor analysis using QUANL PC program. Results: Three different types of role awareness of home health care specialists working at medical institutions were identified. Type I is 'educational-function-centered', Type II 'patient-centered' and Type III 'professional-service-centered'. Conclusion: Regardless of these types, home health care specialists commonly had a high pride as a specialist and a sense of mission regarding themselves as important persons responsible for patients' health.

• 한국간호교육학회지
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• 제23권3호
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• pp.290-299
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• 2017

Purpose: The purpose of this study is to investigate nurses' role conflict and intention to remain and to identify factors that may influence this intention. Methods: Data were collected from 172 nurses in a 600-plus bed hospital on nurses' intention to remain, which included 47 items and nurses' role conflict, which included 82 items from a self-reported questionnaire. Data were analyzed by descriptive statistics and stepwise multiple regression using SPSS 22.0 Results: The mean of the role conflict frequency was 2.71(${\pm}.39$) and the mean severity was 2.86 (${\pm}.47$). The most significant item among the items of intention to remain was "a nurse's job is to help people." The intention to remain employed showed a significant difference in the duration of desired working period and subjective job satisfaction. The subjective job satisfaction of nurses was the most influential variable as a factor affecting the intention to remain, followed by frequency of role conflict in nursing practice. Conclusion: These results suggest that inadequate nursing environments in nursing practice require improvement and support the idea that nurses with positive attitudes of the nursing organization and theirs leaders reduce nurses' related fatigue.