• Asian Pacific Journal of Cancer Prevention
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• 제16권14호
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• pp.5843-5846
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• 2015

Background: Detection of cervical high grade lesions in patients with atypical squamous cells of undetermined significance (ASCUS) is still a challenge. Our study tested the efficacy of the paired boxed gene 1 (PAX1) methylation analysis by methylation-sensitive high-resolution melting (MS-HRM) in the detection of high grade lesions in ASCUS and compared performance with the hybrid capture 2 (HC2) human papillomavirus (HPV) test. Materials and Methods: A total of 463 consecutive ASCUS women from primary screening were selected. Their cervical scrapings were collected and assessed by PAX1 methylation analysis (MS-HRM) and high-risk HPV-DNA test (HC2). All patients with ASCUS were admitted to colposcopy and cervical biopsies. The Chisquare test was used to test the differences of PAX1 methylation or HPV infection between groups. Results: The specificity, sensitivity, and accuracy for detecting CIN2 + lesions were: 95.6%, 82.4%, and 94.6%, respectively, for the PAX1 MS-HRM test; and 59.7%, 64.7%, and 60.0% for the HC2 HPV test. Conclusions: The PAX1 methylation analysis by MS-HRM demonstrated a better performance than the high-risk HPV-DNA test for the detection of high grade lesions (CIN2 +) in ASCUS cases. This approach could screen out the majority of low grade cases of ASCUS, and thus reduce the referral rate to colposcopy.

• Asian Pacific Journal of Cancer Prevention
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• 제13권5호
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• pp.2199-2206
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• 2012

Background: Our objective was to evaluate the MTHFR C677T-A1298C polymorphisms in patients with breast cancer and in individuals with no history of cancer, to compare the levels of genetic damage and apoptosis under folic acid (FA) deficiency between patients and controls, and to assess associations with breast cancer. Methods: Genetic damage was marked by micronucleated binucleated cells (MNBN) and apoptosis was estimated by cytokinesis-block micronucleus assay (CBMN). PCR-RFLP molecular analysis was carried out. Results: The results showed significant associations between the MTHFR 677TT or the combined MTHFR C677T-A1298C and breast cancer risk (OR = 2.51, CI = 0.85 to 7.37, p = 0.08; OR = 4.11, CI = 0.78 to 21.8, p < 0.001). The MNBN from the combined MTHFR C677T-A1298C was higher and the apoptosis was lower than that of the single variants (p < 0.05). At 15 to 60 nmol/L FA, the MNBN in cases with the TTAC genotype was higher than controls (p < 0.05), whereas no significant difference in apoptosis was found between the cases and controls after excluding the genetic background. Conclusions: Associations between the combined MTHFR C677T-A1298C polymorphism and breast cancer are possible from this study. A dose of 120 nmol/L FA could enhance apoptosis in cases with MTHFR C677T-A1298C. Breast cancer individuals with the TTAC genotype may be more sensitive to the genotoxic effects of FA deficiency than controls.

• 한국정밀공학회지
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• 제33권11호
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• pp.943-950
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• 2016

The purpose of this study was to evaluate and compare by finite element analysis the biomechanical performance, in terms of cervical stand-alone cage screw insert angle (Type 3 - 5: 2 Screws) and screw arrangement (Type 6 and 7: 3 Screws / Type 8 and 9: 4 Screws), and the range of motion (ROM) of traditional anterior cervical discectomy of a fusion device (Type 1: Cage / Type 2: Cage + ACP). Our study suggests that the biomechanical behavior of a postoperative cervical spine could indeed be influenced by design features, such as screw angle and number of screws. In particular, ROM and the risk of subsidence were more sensitive during extension about type 5 (Insert Angle $20^{\circ}$). Our study also suggested that the number of screw asymmetries between up and down for type 6 and 7 could result in differences in the risk of screw fracture manifesting in different clinical aspects.

• Pediatric Infection and Vaccine
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• 제25권2호
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• pp.107-112
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• 2018

포도알균은 피부 및 연조직 감염과 관련된 병원체로 재발 감염의 위험이 높다. 저자들은 국내에서 Panton-Valentine leukocidin (PVL) 양성 메티실린 감수성 포도알균 ST1에 의한 가족 내 재발성 종기를 경험하였으며, 16개월의 남자 환아와 아버지, 어머니의 종기에서 획득한 검체는 모두 동일한 항생제 감수성을 보였다. 세 환자의 균주 모두 agr 그룹으로 확인되었고, polymerase chain reaction (PCR) 검사에서 sec, seh가 확인되었으며 PVL 유전자를 포함하고 있었다. 가족 내 신체 접촉으로 인한 포도알균의 전파 가능성 및 예방에 대한 고려를 해 볼 필요가 있다.

• 디지털콘텐츠학회 논문지
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• 제9권3호
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• pp.491-498
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• 2008

최근 몇 년간 악의적 내부자에 의해 기업의 기밀정보가 유출된 사례들이 급속도로 증가하고 있으며, 이는 기업의 경쟁력 약화와 심지어 기업의 생존을 좌우하고 있음에도 불구하고 내부자에 의한 기밀정보 유출을 대비한 보안대책과 보안기술은 미비한 실정이다. 이에, 본 논문에서는 현재 구축 운영 중인 기업 내부 지향적인 보안 기술들에 대한 특징과 한계들을 분석하여 기업정보 유출 사고 발생 시 그 피해를 최소화하기 위한 통합 로그분석 시스템을 제안한다. 통합 로그분석 시스템은 사전에 분석된 위협 룰에 근거하여 내부 구성원의 기밀정보 유출 가능 행위들을 사전에 탐지하는 시스템으로, 보안 및 업무단위 시스템을 연동하여 사용자의 모든 행위 로그를 수집하는 로그수집 모듈, 룰에 의해 비정상 유출행위를 탐지하며 보안지수를 생성하는 로그분석 모듈, 탐지 결과 보고서를 생성하는 모듈로 구성된다.

• 방사선산업학회지
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• 제3권1호
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• pp.25-29
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• 2009

Microarrays can be used to screen thousands of binding events in a parallel and high throughput fashion and are of major importance in disease diagnosis and drug discovery. The use of radioisotope is conventionally regarded as one of the most sensitive detection methods. Atherosclerosis is a common disorder affecting arterial blood vessels. It happens when fat, cholesterol, and other substances made in the arterial blood vessels form a hard substances called plaque. Lipoprotein-associated phospholipase $A_2$ ($Lp-PLA_2$), a phospholipase $A_2$ enzyme, is used as a marker for cardiac disease. The detection of $Lp-PLA_2$ was accomplished by using radioactive [$^3H-acetyl$] PAF as a substrate and a feasibility study on RI biochip application to detection of $Lp-PLA_2$, a risk factors of atherosclerosis was performed. Inhibitive activity of a native plant extract was also determined by using the RI biochip. It was found to be applicable to a high-throughput screening of inhibitors for developing atherosclerosis therapeutic agents.

• 한국환경보건학회지
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• 제45권1호
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• pp.18-29
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• 2019

Objectives: The purpose of this study was to examine the relationship between urinary bisphenol A concentration and allergic diseases in children. Methods: This study was conducted in Seoul, South Korea. We collected urine samples from 231 children from a single elementary school in June 2014. Among these, 69 children with urinary creatinine levels outside the normal range were excluded. Information on allergic diseases was obtained from the parents of the children. Urinary bisphenol A was analyzed using a liquid chromatography tandem mass spectrometer. Logistic regression analysis was used to determine if allergic disease was affected by urinary bisphenol A concentration. Results: Girls had a significantly higher concentration of urinary bisphenol A than did boys (p<0.05). Children of 10-12 years old had a significantly higher concentration of urinary bisphenol A than did children 7-9 years old (p<0.01). Concentration of urinary bisphenol A was increased from underweight to overweight (p<0.05). As the concentration of urinary bisphenol A was increased by $1{\mu}g/L$ or $1{\mu}g/g$ creatinine, the risks of lifetime symptoms of atopic dermatitis in children was 1.22 times (95% CI; 1.05-1.41) or 1.08 times (95% CI; 1.01-1.15). Conclusion: Concentration of urinary bisphenol A was associated with gender, age, body mass index, and allergic disease. Particularly, urinary bisphenol A concentration was associated with lifetime symptoms of atopic dermatitis. The findings of this study could contribute to the management of health effects among sensitive groups such as children.

• 실내환경 및 냄새 학회지
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• 제17권4호
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• pp.337-345
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• 2018

In this study, we measured the concentration of Particulate Matter($PM_{10}$), Formaldehyde(HCHO), and Total Bacteria Count (TBC) at two facilities: day care centers, and postnatal care centers located in the cities of Gyeonggi, Gangwon, Jeolla and Gyeongsang from January 1, 2012 to December 31, 2015. $PM_{10}$ concentration was similar to the day care centers and postnatal care centers. HCHO concentration was the highest in the postnatal care centers. TBC concentration was the highest in the day care centers. Comparing the different cities, $PM_{10}$ concentration was the highest in Gyeonggi, HCHO concentration was the highest in Gyeonggi, and TBC concentration was the highest in Gyeonggi. As a result of HCHO's risk assessment, it was found that adults exceeded the carcinogenicity tolerance of $10^{-6}$ specified by the US EPA. This study is expected to be helpful in preventing damage to health from the contaminated indoor air at sensitive facilities, and can be used as basic data for indoor air quality management.

• 한국융합학회논문지
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• 제12권3호
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• pp.317-323
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• 2021

본 연구의 목적은 청소년의 체질량지수에 따른 구강증상 경험을 분석하고자 함이다. 청소년건강행태조사 자료를 이용하여 총 55,728명을 대상으로 복합표본분석을 실시하였다. 민감성 치아, 치아통증, 치은출혈 및 부종의 경험은 각각 과체중 그룹(36.8%, 23.9%, 19.2%)에서 가장 높고 저체중 그룹(34.6%, 20.8%, 17.0%)은 가장 낮았으며, 구취경험은 비만 그룹이 26.7%로 가장 높고 저체중 그룹은 18.8%로 가장 낮았다(p<0.001). 정상체중을 기준으로 했을 때 치아통증 경험은 과체중 그룹이 1.08배(95%CI: 1.01-1.15) 유의하게 높았고 치은출혈 및 부종 또한 과체중 그룹에서 1.08배(95%CI: 1.01-1.16) 유의하게 높았다. 구취경험은 과체중 그룹에서 1.19배(95%CI: 1.11-1.27), 비만 그룹에서 1.43배(95%CI:1.35-1.52) 유의하게 높았다. 차후 전신건강과 구강건강에 관한 보다 다양한 연구가 필요할 것이다.

• Clinics in Shoulder and Elbow
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• 제26권2호
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• pp.182-190
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• 2023

Elbow traumas represent a relatively common condition in clinical practice. However, there is a lack of evidence regarding the most accurate tests for screening these potentially serious conditions and excluding elbow fractures. The purpose of this investigation was to analyze the literature concerning the diagnostic accuracy of clinical tests for the detection or exclusion of suspected elbow fractures. A systematic review was performed using the Preferred Reporting Items for a Systematic Review and Meta-analysis of Diagnostic Test Accuracy Studies (PRISMA-DTA) guidelines. Literature databases including PubMed, Cumulative Index to Nursing and Allied Health Literature, Diagnostic Test Accuracy, Cochrane Library, the Web of Science, and ScienceDirect were searched for diagnostic accuracy studies of subjects with suspected traumatic elbow fracture investigating clinical tests compared to imaging reference tests. The risk of bias in each study was assessed independently by two reviewers using the Quality Assessment of Diagnostic Accuracy Studies 2 checklist. Twelve studies (4,485 patients) were included. Three different types of index tests were extracted. In adults, these tests were very sensitive, with values up to 98.6% (95% confidence interval [CI], 95.0%-99.8%). The specificity was very variable, ranging from 24.0% (95% CI, 19.0%-30.0%) to 69.4% (95% CI, 57.3%-79.5%). The applicability of these tests was very high, while overall studies showed a medium risk of bias. Elbow full range of motion test, elbow extension test, and elbow extension and point tenderness test appear to be useful in the presence of a negative test to exclude fracture in a majority of cases. The specificity of all tests, however, does not allow us to draw useful conclusions because there was a great variability of results obtained.