• 한국산림과학회지
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• 제112권2호
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• pp.258-266
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• 2023

소나무재선충병(Pine wilt disease)은 소나무재선충(Bursaphelenchus xylophilus (Steiner & Buhrer, 1934))에 의해 발생하며 한국을 비롯한 동북아시아와 유럽의 소나무속(Pinus) 수목들을 고사시키고 있다. 소나무재선충병 저항성에 대한 연구는 자연상태 및 온실포트묘에서 주로 이뤄진 반면 포지에서의 연구가 부족한 편이다. 본 연구에서는 2021년 6월, 포지에 있는 소나무속 수종인 곰솔(Pinus thunbergii Parl.), 잣나무(P. koraiensis Siebold & Zucc.), 소나무(P. densiflora Siebold & Zucc.), 섬잣나무(P. parviflora Siebold & Zucc.), 리기테다소나무(P. rigida × P. taeda), 스트로브잣나무(P. strobus L.)의 묘목을 대상으로 소나무재선충을 인공접종하였다. 그리고 인공접종 후 2주 간격으로 위조지수를 측정했고, 인공접종 24주후의 고사율, 소나무재선충 검출률 및 밀도를 측정했다. 소나무재선충병에 의한 고사율은 곰솔(80%), 잣나무(77.8%), 소나무(62.5%), 섬잣나무(22.0%) 순이였으며 리기테다소나무와 스트로브잣나무는 고사하지 않았다. 소나무재선충 검출률은 리기테다소나무(22.2%)를 제외하고 모두 고사율과 동일했다. 위조지수의 경우, 소나무재선충병 고사율 순위가 높았던 곰솔, 잣나무, 소나무 3종에서 높은 수치가 나왔으며, 순위가 낮았던 섬잣나무, 리기테다소나무, 스트로브잣나무 3종에서는 대조군과 차이가 없었다. 통계분석 결과, 곰솔, 잣나무는 소나무재선충병에 높은 감수성을, 소나무, 섬잣나무는 중간 수준의 감수성을, 리기테다소나무와 스트로브잣나무는 확실한 저항성을 보여주는 것으로 나타났다. 본 연구는 소나무재선충 저항성 육종의 기초자료나리기테다소나무와 스트로브잣나무의 조림 필요성에 대한 근거자료가 될 수 있을 것이다.

• Applied Microscopy
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• 제42권3호
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• pp.147-150
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• 2012

A recently introduced helium ion microscopy (HIM) was employed to observe uncoated pine leaf specimens. Adult leaves were collected from the seedlings of Pinus densiflora and P. rigida, air-dried at room temperature, and observed by HIM without metal coating. Ovoid or round stomata and distinct Florin rings could be discerned. The epicuticular waxes were present in the epistomatal chambers and Florin rings of stomata on the leaf surface. The epicuticular waxes were mostly straight, cylindrical, and ca. 1 ${\mu}m$ in length. The epistomatal chambers of P. rigida were filled with the epicuticular waxes, whereas those of P. densiflora were not filled with the epicuticular waxes. Based on their micromorphology, the epicuticular wax structures of the pine species were identified as tubules. These results suggest that the HIM could be used for the investigation of the plant stomata and epicuticular waxes of uncoated plant leaves. Due to the smaller ion probe and interaction volume, the HIM has advantages over conventional field emission scanning electron microscopy in terms of image resolution and charge neutralization.

• 한국산림과학회지
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• 제94권5호통권162호
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• pp.302-306
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• 2005

Litterfall and nutrient inputs were measured in even-aged coniferous plantations (a 31-year-old Pinus rigida and a 31-year-old Larix leptolepis) on a similar site condition in the Forest Practice Research Center, Gyeonggi Province. Litterfall was collected monthly from circular littertraps (collecting area: $0.50m^2$) for three years between April 1997 and February 2000. Average total annual litterfall was significantly higher for pine (5,802 kg/ha/yr) than for larch (4,562 kg/ha/yr) plantations. Needle litter in both plantations accounted for about 63% of total litterfall. Litterfall in the larch was distributed as follows: needle > other leaf > branch > miscellaneous > bark, while it was needle > miscellaneous > other leaf > branch > bark in the pine plantation. There was no temporal variation in needle litter, other leaf and bark during the 3 year study period. The concentrations of all nutrients (N, P, K, Ca, Mg) in needle litter were significantly higher in the larch than in the pine plantations. The annual nutrient concentration of needle litter in the larch varied among the years, whereas no year variation of needle litter was in the pine except for phosphorus (P). Nitrogen (N) and P inputs by needle litter were significantly higher for larch than for pine plantations established on a similar soil. The differences in N and P inputs were attributed to lower nutrient concentration in pine needle litter compared with larch needle litter, not to total needle litter mass. Annual inputs of nutrient in both plantations were not significantly different among years except for K of the larch although there was yearly different in needlefall mass and nutrient concentration during the 3-year observed period. The results indicate that the mechanisms of litterfall and nutrient inputs vary considerably between pine and larch plantations established on a similar site condition.

• The Korean Journal of Ecology
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• 제25권6호
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• pp.389-393
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• 2002

Effects of environmental factors on in vitro pine pollen performance were investigated. Pinus densiflora and P. rigida pollen grains collected at Mt. Kwanak, Korea were used. Three environmental factors, such as pollen storage temperature, pollen culture temperature and nutrient condition in medium, were tested. To determine the storage temperature effects on pollen viability, pine pollen was stored at $-70^{\circ}C$, $-12^{\circ}C$, $4^{\circ}C$ and $22^{\circ}C$. Pollen viability was substantially extended at the storage temperatures of $-12^{\circ}C$ and $4^{\circ}C$ for more than 300 days. To elucidate the culture temperature effects on pine pollen germination and tube growth, pollen grains were cultured at the temperatures from $5^{\circ}C$ to $40^{\circ}C$ at $5^{\circ}C$intervals. The germination rate and tube growth were highest at $25^{\circ}C$ and decreased above $30^{\circ}C$. To investigate boron and sucrose effects on pollen tube growth, the pollen was cultured at different sucrose and boric acid concentrations. Germination rate was optimal in germination medium containing 3 or 5$\%$ sucrose with 0.01 $\%$ boric acid. These results indicate that the pine pollen can be stored for considerable length of time without noticeable loss of viability at storage temperature below or near $0^{\circ}C$. Optimal germination medium conditions were established for pine pollen. Therefore, pine pollen can be used for many biological and environmental monitoring researches.

• Journal of Ecology and Environment
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• 제32권1호
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• pp.27-31
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• 2009

Organic carbon (OC) distribution in 32-year-old pitch pine plantation at Mt. Hotae in Kongju, Korea, was studied from August 2007 to July 2008. In order to investigate the OC distribution, OC in plant biomass, litterfall, litter layer on forest floor, and soil within 50cm depth were estimated. The density of P. rigida plantation was 3,200 trees/ha, average DBH was $18.7{\pm}5.53cm$ and average tree height was $11.1{\pm}1.85m$. Organic carbon stored in plant biomass, litterlayer on forest floor and soil in 2008 was 89.46 ton C/ha (46.09%), 4.32 ton C/ha (2.23%) and 100.32 ton C $ha^{-1}$ 50cm-$depth^{-1}$ (51.68%), respectively. Amount of OC returned to forest floor via litterfall was 2.21 ton C $ha^{-1}\;yr^{-1}$. Total amount of OC stored in this P. rigida plantation was 194.1 ton C/ha. Net increase of OC in above- and below-ground biomass in this pitch pine plantation was 4.82 ton C $ha^{-1}\;yr^{-1}$.

• 한국연초학회지
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• 제24권1호
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• pp.53-59
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• 2002

This study was conducted to evaluate whether pine needle extracts can be used as tobacco flavors. Yield of essential oil, absolute and oleoresin extracted from pine needles is 0.07%, 1.20% and 6.08% respectively. The volatile compounds isolated from the three types of extracts were analyzed by gas chromatography(GC) and mass selective detector(MSD). Total 72 components were identified in the three type of extracts including 26 hydrocarbons, 16 alcohols, 13 esters, 9 acids, 4 phenols, 2 aldehydes and 2 ketones compounds. The major components were $\beta$-pinene, $\beta$-caryophyllene, $\delta$-cadinene and 4,5-dimethyl-1,3 -dioxol-2-one. There were 49 volatile components in the absolute, 44 components in the essential oil and 26 components in the oleoresin. The content of hydrocarbons and alcohols was higher in the essential oil extracted by simultaneous distillation extraction(SDE) than in others, while that of esters and acids was higher in the absolute than in others. Especially, phenols and ketones were identified only in the oleoresin. The components such as $\beta$-pinene, bornyl acetate, $\alpha$-terpineol and oxygenated terpenes have characteristic piney and fresh green odor. The contents of these components was higher in the essential oil and the absolute than in the oleoresin. Therefor, the essential oil and the absolute are expected to be more useful than the oleoresin as tobacco flavor.

• 한국환경생물학회:학술대회논문집
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• 한국환경생물학회 2005년도 추계학술대회 초록집
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• pp.134-134
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• 2005

• 한국안전학회지
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• 제30권1호
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• pp.111-118
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• 2015

In this study, we carried out the emissions analysis of CO and $non-CO_2$ for the age-classes of various pine trees(Pinus koraiensis, Pinus densiflora, Pinus rigida Mill., Pinus thunbergii Parl.) to estimate of emission factors of the crown layer and surface layer in the forest fire. We used the thermal characteristic analyzer cone heater and NDIR analyzer in order to measure amount of emission. As a result, the major emissions of Pinus koraiensis were $CO_2$ and $CH_4$ and that of Pinus thunbergii Parl. was only CO. The major emissions of the most of pine trees were NO and $N_2O$. The $CO_2$ emission of Pinus thunbergii Parl. was the highest about as $7.26{\times}10^{-2}{\sim}1.63{\times}10^{-1}g$ and next came Pinus densiflora, Pinus koraiensis, Pinus rigida Mill.. And the CO emission of Pinus thunbergii Parl. was about $5.14{\times}10^{-3}{\sim}6.58{\times}10^{-3}g$ and followed by Pinus densiflora, Pinus koraiensis, Pinus rigida Mill.. The emissions of $CH_4$, NO, and $N_2O$ showed small differences between species and the emission of $CH_4$ was $8.37{\times}10^{-5}{\sim}2.55{\times}10^{-4}g$, and NO was $6.65{\times}10^{-5}{\sim}2.0{\times}10^{-4}g$ and $N_2O$ was $1.42{\times}10^{-4}{\sim}2.09{\times}10^{-3}g$ in all species. Particularly, the emission of Pinus thunbergii Parl. was the highest in all pine trees except $CH_4$.

• The Korean Journal of Ecology
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• 제17권3호
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• pp.345-353
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• 1994

상수리나무림과 리기다소나무에서 낙엽 생산과 분해를 조사하였다. 낙엽 생산량은 litter trap을, 낙엽의 분해는 litterbag를 이용하여 정량적으로 조사하였다. 낙엽의 생산은 연중 계속 되었지만 가을에 최대치를 보였으며, 5월과 6월에는 생식기관이나 눈을 싸고 있던 비늘에 의해 두 번째의 peak가 나타났다. 연간 낙엽 생산량은 상수리나무림과 리가다소나무림에서 각각 567.1g/m2과 653.2g/m2이었다. 본 조사지역의 낙엽 생산량은 다른 지역의 결과에 비해 많았다. 낙엽의 영양염류 함량은 상수리나무가 리기다소나무에 비해 높았고, 칼슘을 제외하고 낙엽생산량이 최대인 가을에 그 값이 낮았다. 1년이 지난 후 낙엽의 잔존률은 상수리나무와 리기다소나무가 각각 43.6%, 58%이었고, 21개월 후에는 그 값이 각각 22.2%와 33.2% 이었다.

• Journal of the Korean Wood Science and Technology
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• 제15권2호
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• pp.53-66
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• 1987

Korean red pine (Pinus densiflora S. et. Z.) and pitch pine(Pinus rigida Mill) $5{\times}10cm$ dimension lumber were dried in a kiln providing a cross-circulation velocity of 5 m/sec at dry-and wet-bulb temperatures of 116 and $71^{\circ}C$, followed by 3 hours at 91 and $85^{\circ}C$. Compared to dimension lumber dried lumber were as follows. 1. To dry to 10 percent moisture content, the high-temperatures schedule of Korean red pine and pitch pine lumber took less than one seventh the time required by the conventional kiln drying schedule. 2. High-temperature drying rate and conventional drying rate to 10 percent moisture content of Korean red pine lumber were 2.75 and 0.35%/hr, and those of pitch pine lumber were 3.38 and 0.46%/hr respectively. 3. Compared to lumber of both species on conventional schedule, moisture gradient of high-temperature lumber was greater. 4. Compared to lumber on conventional schedule, maximum surface checking of high-temperature lumber of both species was severer, and maximum end checking of high-temperature lumber of both species was similar to that of lumber on conventional schedule. 5. Compard to lumber on conventional schedule, Korean red pine lumber dried at high temperature showed more honeycombing, but pitch pine lumber dried at high-temperature showed significantly slighter honeycombing. 6. Compared to lumber on conventional schedule, the high-temperature lumber showed less warping lumber of both species. 7. Collapse and casehardening of Korean red pine and pitch pine lumber on both scheules were slight.