• Journal of Korean Society of Forest Science
• /
• v.112 no.2
• /
• pp.258-266
• /
• 2023

Pine wilt disease is caused by the pine wood nematode (Bursaphelenchus xylophilus) and has killed many pine trees in Europe and Northeast Asia, including South Korea. Resistance to pine wilt disease varies among species. Previous studies were mostly conducted in nature or greenhouses and only a few in test fields. In this study, seedlings of six pine species (Pinus thunbergii, P. koraiensis, P. densiflora, P. parviflora, P. rigida × P. taeda, and P. strobus) were artificially inoculated by pine wood nematodes in the test field. The Wilt Index was measured every 2 weeks after inoculation in addition to the mortality rate, detection rate, and pine wood nematode concentration measurement after 24 weeks. The pine wilt disease mortality rates were P. thunbergii (80%), P. koraiensis (77.8%), P. densiflora (62.5%), and P. parviflora (22.0%), and both P. rigida × P. taeda and P. strobus survived. The pine nematode detection rates were the same among the species except for P. rigida × P. taeda pine (22.2%). High Wilt-Index values were obtained for P. thunbergii, P. koraiensis, and P. densiflora, which had mortality rates higher than the other species. Furthermore, there were no significant differences in the Wilt Indexes between P. parviflora, P. rigida × P. taeda, P. strobus, and the control group. Statistically, P. thunbergii and P. koraiensis showed high susceptibility to pine wilt disease, P. densiflora and P. parviflora showed moderate susceptibility, and P. rigida × P. taeda and P. strobus showed apparent resistance. These results provide basic data for pine wood nematode resistance breeding or as evidence of the need for afforestation of P. rigida × P. taeda and P. strobus.

• Applied Microscopy
• /
• v.42 no.3
• /
• pp.147-150
• /
• 2012

A recently introduced helium ion microscopy (HIM) was employed to observe uncoated pine leaf specimens. Adult leaves were collected from the seedlings of Pinus densiflora and P. rigida, air-dried at room temperature, and observed by HIM without metal coating. Ovoid or round stomata and distinct Florin rings could be discerned. The epicuticular waxes were present in the epistomatal chambers and Florin rings of stomata on the leaf surface. The epicuticular waxes were mostly straight, cylindrical, and ca. 1 ${\mu}m$ in length. The epistomatal chambers of P. rigida were filled with the epicuticular waxes, whereas those of P. densiflora were not filled with the epicuticular waxes. Based on their micromorphology, the epicuticular wax structures of the pine species were identified as tubules. These results suggest that the HIM could be used for the investigation of the plant stomata and epicuticular waxes of uncoated plant leaves. Due to the smaller ion probe and interaction volume, the HIM has advantages over conventional field emission scanning electron microscopy in terms of image resolution and charge neutralization.

• Journal of Korean Society of Forest Science
• /
• v.94 no.5 s.162
• /
• pp.302-306
• /
• 2005

Litterfall and nutrient inputs were measured in even-aged coniferous plantations (a 31-year-old Pinus rigida and a 31-year-old Larix leptolepis) on a similar site condition in the Forest Practice Research Center, Gyeonggi Province. Litterfall was collected monthly from circular littertraps (collecting area: $0.50m^2$) for three years between April 1997 and February 2000. Average total annual litterfall was significantly higher for pine (5,802 kg/ha/yr) than for larch (4,562 kg/ha/yr) plantations. Needle litter in both plantations accounted for about 63% of total litterfall. Litterfall in the larch was distributed as follows: needle > other leaf > branch > miscellaneous > bark, while it was needle > miscellaneous > other leaf > branch > bark in the pine plantation. There was no temporal variation in needle litter, other leaf and bark during the 3 year study period. The concentrations of all nutrients (N, P, K, Ca, Mg) in needle litter were significantly higher in the larch than in the pine plantations. The annual nutrient concentration of needle litter in the larch varied among the years, whereas no year variation of needle litter was in the pine except for phosphorus (P). Nitrogen (N) and P inputs by needle litter were significantly higher for larch than for pine plantations established on a similar soil. The differences in N and P inputs were attributed to lower nutrient concentration in pine needle litter compared with larch needle litter, not to total needle litter mass. Annual inputs of nutrient in both plantations were not significantly different among years except for K of the larch although there was yearly different in needlefall mass and nutrient concentration during the 3-year observed period. The results indicate that the mechanisms of litterfall and nutrient inputs vary considerably between pine and larch plantations established on a similar site condition.

• The Korean Journal of Ecology
• /
• v.25 no.6
• /
• pp.389-393
• /
• 2002

Effects of environmental factors on in vitro pine pollen performance were investigated. Pinus densiflora and P. rigida pollen grains collected at Mt. Kwanak, Korea were used. Three environmental factors, such as pollen storage temperature, pollen culture temperature and nutrient condition in medium, were tested. To determine the storage temperature effects on pollen viability, pine pollen was stored at $-70^{\circ}C$, $-12^{\circ}C$, $4^{\circ}C$ and $22^{\circ}C$. Pollen viability was substantially extended at the storage temperatures of $-12^{\circ}C$ and $4^{\circ}C$ for more than 300 days. To elucidate the culture temperature effects on pine pollen germination and tube growth, pollen grains were cultured at the temperatures from $5^{\circ}C$ to $40^{\circ}C$ at $5^{\circ}C$intervals. The germination rate and tube growth were highest at $25^{\circ}C$ and decreased above $30^{\circ}C$. To investigate boron and sucrose effects on pollen tube growth, the pollen was cultured at different sucrose and boric acid concentrations. Germination rate was optimal in germination medium containing 3 or 5$\%$ sucrose with 0.01 $\%$ boric acid. These results indicate that the pine pollen can be stored for considerable length of time without noticeable loss of viability at storage temperature below or near $0^{\circ}C$. Optimal germination medium conditions were established for pine pollen. Therefore, pine pollen can be used for many biological and environmental monitoring researches.

• Journal of Ecology and Environment
• /
• v.32 no.1
• /
• pp.27-31
• /
• 2009

Organic carbon (OC) distribution in 32-year-old pitch pine plantation at Mt. Hotae in Kongju, Korea, was studied from August 2007 to July 2008. In order to investigate the OC distribution, OC in plant biomass, litterfall, litter layer on forest floor, and soil within 50cm depth were estimated. The density of P. rigida plantation was 3,200 trees/ha, average DBH was $18.7{\pm}5.53cm$ and average tree height was $11.1{\pm}1.85m$. Organic carbon stored in plant biomass, litterlayer on forest floor and soil in 2008 was 89.46 ton C/ha (46.09%), 4.32 ton C/ha (2.23%) and 100.32 ton C $ha^{-1}$ 50cm-$depth^{-1}$ (51.68%), respectively. Amount of OC returned to forest floor via litterfall was 2.21 ton C $ha^{-1}\;yr^{-1}$. Total amount of OC stored in this P. rigida plantation was 194.1 ton C/ha. Net increase of OC in above- and below-ground biomass in this pitch pine plantation was 4.82 ton C $ha^{-1}\;yr^{-1}$.

• Journal of the Korean Society of Tobacco Science
• /
• v.24 no.1
• /
• pp.53-59
• /
• 2002

This study was conducted to evaluate whether pine needle extracts can be used as tobacco flavors. Yield of essential oil, absolute and oleoresin extracted from pine needles is 0.07%, 1.20% and 6.08% respectively. The volatile compounds isolated from the three types of extracts were analyzed by gas chromatography(GC) and mass selective detector(MSD). Total 72 components were identified in the three type of extracts including 26 hydrocarbons, 16 alcohols, 13 esters, 9 acids, 4 phenols, 2 aldehydes and 2 ketones compounds. The major components were $\beta$-pinene, $\beta$-caryophyllene, $\delta$-cadinene and 4,5-dimethyl-1,3 -dioxol-2-one. There were 49 volatile components in the absolute, 44 components in the essential oil and 26 components in the oleoresin. The content of hydrocarbons and alcohols was higher in the essential oil extracted by simultaneous distillation extraction(SDE) than in others, while that of esters and acids was higher in the absolute than in others. Especially, phenols and ketones were identified only in the oleoresin. The components such as $\beta$-pinene, bornyl acetate, $\alpha$-terpineol and oxygenated terpenes have characteristic piney and fresh green odor. The contents of these components was higher in the essential oil and the absolute than in the oleoresin. Therefor, the essential oil and the absolute are expected to be more useful than the oleoresin as tobacco flavor.

• Proceedings of the Korea Society of Environmental Biology Conference
• /
• 2005.12a
• /
• pp.134-134
• /
• 2005

• Journal of the Korean Society of Safety
• /
• v.30 no.1
• /
• pp.111-118
• /
• 2015

In this study, we carried out the emissions analysis of CO and $non-CO_2$ for the age-classes of various pine trees(Pinus koraiensis, Pinus densiflora, Pinus rigida Mill., Pinus thunbergii Parl.) to estimate of emission factors of the crown layer and surface layer in the forest fire. We used the thermal characteristic analyzer cone heater and NDIR analyzer in order to measure amount of emission. As a result, the major emissions of Pinus koraiensis were $CO_2$ and $CH_4$ and that of Pinus thunbergii Parl. was only CO. The major emissions of the most of pine trees were NO and $N_2O$. The $CO_2$ emission of Pinus thunbergii Parl. was the highest about as $7.26{\times}10^{-2}{\sim}1.63{\times}10^{-1}g$ and next came Pinus densiflora, Pinus koraiensis, Pinus rigida Mill.. And the CO emission of Pinus thunbergii Parl. was about $5.14{\times}10^{-3}{\sim}6.58{\times}10^{-3}g$ and followed by Pinus densiflora, Pinus koraiensis, Pinus rigida Mill.. The emissions of $CH_4$, NO, and $N_2O$ showed small differences between species and the emission of $CH_4$ was $8.37{\times}10^{-5}{\sim}2.55{\times}10^{-4}g$, and NO was $6.65{\times}10^{-5}{\sim}2.0{\times}10^{-4}g$ and $N_2O$ was $1.42{\times}10^{-4}{\sim}2.09{\times}10^{-3}g$ in all species. Particularly, the emission of Pinus thunbergii Parl. was the highest in all pine trees except $CH_4$.

• The Korean Journal of Ecology
• /
• v.17 no.3
• /
• pp.345-353
• /
• 1994

Litter production and decomposition were investigated for 2 years in the oak, Quercus acutissima, and the pitch pine, Pinus rigida, stands in the vicinity of Kongju, Chungnam Province. Litter production was measured with litter trap at monthly basis. Litterbag method was used for the measurement of litter decomposition. Litter producion continued throughout the year, but showed a peak in autumn. Second peak in May or June was caused by falling of bud scales and reproductive organs. Average litter production in the oak and the pitch pine stands were $567.1g{\cdot}m^{-2}{\cdot}yr^{-1}\;and\;653.2g{\cdot}m^{-2}{\cdot}yr^{-1}$, respectively. Litter production in this study area were higher than those in other reports. Nutrient concentrations in litter were the highest in summer when the least litter production occurred, and the lowest in autumn when the greatest litter production occurred, except for calcium in the oak stand. Nutrient concentrations of the oak litter were higher than those in the pitch pine litter. After 1 year, % remaining mass of oak and pitch pine litter was 43.6% and 58%, respectively. After 21 months elapsed, % remaining mass of oak and pitch pine litter was 22.2% and 33.2%, respectively.

• Journal of the Korean Wood Science and Technology
• /
• v.15 no.2
• /
• pp.53-66
• /
• 1987

Korean red pine (Pinus densiflora S. et. Z.) and pitch pine(Pinus rigida Mill) $5{\times}10cm$ dimension lumber were dried in a kiln providing a cross-circulation velocity of 5 m/sec at dry-and wet-bulb temperatures of 116 and $71^{\circ}C$, followed by 3 hours at 91 and $85^{\circ}C$. Compared to dimension lumber dried lumber were as follows. 1. To dry to 10 percent moisture content, the high-temperatures schedule of Korean red pine and pitch pine lumber took less than one seventh the time required by the conventional kiln drying schedule. 2. High-temperature drying rate and conventional drying rate to 10 percent moisture content of Korean red pine lumber were 2.75 and 0.35%/hr, and those of pitch pine lumber were 3.38 and 0.46%/hr respectively. 3. Compared to lumber of both species on conventional schedule, moisture gradient of high-temperature lumber was greater. 4. Compared to lumber on conventional schedule, maximum surface checking of high-temperature lumber of both species was severer, and maximum end checking of high-temperature lumber of both species was similar to that of lumber on conventional schedule. 5. Compard to lumber on conventional schedule, Korean red pine lumber dried at high temperature showed more honeycombing, but pitch pine lumber dried at high-temperature showed significantly slighter honeycombing. 6. Compared to lumber on conventional schedule, the high-temperature lumber showed less warping lumber of both species. 7. Collapse and casehardening of Korean red pine and pitch pine lumber on both scheules were slight.