• Applied Biological Chemistry
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• v.41 no.8
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• pp.595-599
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• 1998

An analytical method was developed to determine tricyclazole residues in rice grain, straw, and soil using high-performance liquid chromatography (HPLC) with ultraviolet absorption detection. Tricyclazole was extracted with methanol from moist rice grain, straw, and soil samples. n-Hexane washing was employed to remove nonpolar co-extractives during liquid-liquid partition. Tricyclazole was then extracted with dichloromethane from alkaline aqueous phase, while acidic interferences remained in the phase. Dichloromethane extract was further purified by silica gel column chromatography prior to HPLC determination. Reverse-phase HPLC using an octadecylsilyl column was successfully applied to separate and quantitate the tricyclazole residue in sample extracts monitored at ${\lambda}_{max}$ 225nm. Recoveries from fortified samples averaged $95.5{\pm}3.0%\;(n=6),\;87.5{\pm}20.%\;(n=6),\;and\;84.3{\pm}2.8%$ (n=12) for rice grain, straw, and soil, respectively. Detection limit of the method was 0.02 mg/kg for rice grain and soil samples while 0.05 mg/kg for rice straw samples. The proposed method was reproducible and sensitive enough to evaluate the safety of tricyclazole residues in rice grain, straw, and soil.

• Asian-Australasian Journal of Animal Sciences
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• v.2 no.2
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• pp.115-121
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• 1989

A comparative slaughter feeding experiment with steer calves weighing 280 kg and a concurrent digestion trial with wether lambs was conducted to study the energy value of rice straw as influenced by ammonia treatment and mixing with alfalfa hay. Steers were ad libitum fed one of nine completely mixed experimental diets: basal (high concentrate); 25 or 50% of untreated rice straw (URS) or ammoniated (plastic covered bales, 4.6% $NH_3$ by weight) rice straw (ARS) proportionately replacing part of the basal; a 50:50 % mixture of URS or ARS and alfalfa replacing a proportion of the basal at 25 and 50%. Digestibility of the nine complete diets (pelleted to prevent sorting) was determined with four ad libitum fed lambs. Ammoniation increased crude protein level (from 3.6 to 10.8%) and in vitro dry matter digestibility of the rice straw by 15%. The improvement in DE, NEm and NEg by ammoniation of rice straw was 20, 52 and 117%, respectively. Ammoniation of rice straw fed as 50% of the diet improved gains over the diet containing 50% URS, but no significant influence on animal performance was observed when rice straw was fed at the 25% level. Each 10% addition of URS to basal diet decreased empty body gain of steers by 116 gram per day compared with a decrease of 70 gram per day when rice straw had been ammoniated. There was no beneficial effect of ammoniation when the roughage component of the diet was a 50:50 mixture of rice straw and alfalfa. Ammoniation of straw and inclusion of alfalfa generally increased the concentration of total volatile fatty acids in rumen fluid. Ammoniation resulted in reduced concentrations of acetic and propionic acid, but increased concentration of butyric acid. Digestibility of URS was improved by mixing with alfalfa. However, alfalfa hay did not influence digestibility of ARS. Diets in which ARS replaced the basal mixture at 25 and 50% had higher NEm and NEg values than comparable URS diets. The same pattern was observed in the straw: alfalfa mixtures, but differences between URS and ARS were significant only for the 50% roughage diets.

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.6
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• pp.807-817
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• 2008

Steers with ad libitum access to rice straw were assigned to four diets to evaluate the effects of maize or soybean hull supplementation on intake, in vivo digestibility, ruminal pH, VFA, ammonia-nitrogen ($NH_3-N$) and in situ ruminal disappearance of feed nutrients by cattle consuming rice straw. Supplement treatments were: no supplement (RS); soybean meal at 0.127% BW (SBM); cracked maize at 0.415% BW plus 0.044% BW soybean meal (MAIZE); or soybean hulls at 0.415% BW plus 0.044% BW soybean meal (HULLS). The MAIZE and HULLS diets were formulated to provide approximately 4 MJ of $NE_m$ per kg of diet. Rice straw DMI was not affected (p = 0.34) by supplement. Apparent dry matter (DM) digestibility was greater (p<0.001) for MAIZE and HULLS (56.6 and 60.0%, respectively) than for steers consuming SBM or RS (51.8 and 44.4%, respectively). Apparent NDF digestibility was greater (p<0.0004) for HULLS than MAIZE (61.7 vs. 58.0%, respectively) and apparent ADF digestibility was greater (p<0.0008) for HULLS than MAIZE (61.1 vs. 49.2%, respectively). There was no difference in apparent hemicellulose digestibility (p = 0.43). Analysis of ruminal fluid collected 0, 2, 4, 6, and 8 h post-feeding revealed ammonia-nitrogen was greatest (p<0.05) for steers on SBM and HULLS diets at 2 h (24.08 and 22.57 mg/dl, respectively) and total volatile fatty acids was greatest (p<0.05) for HULLS at 4 h (230 mM/L). In situ disappearance, measured at 0, 2, 4, 6, 8, 16 and 24 h, indicated that SBM, MAIZE and HULLS tended to enhance the digestibility of DM and fiber components of rice straw. In situ disappearance of rice straw DM was greatest for SBM and/or HULLS from 4 to 24 h (p = 0.03). Rice straw NDF and ADF disappearance was enhanced by supplementation from 16 to 24 h (p<0.02). Rice straw DM, NDF and ADF disappearances at 24 h were similar for MAIZE and HULLS treatments. When feeding cattle rice straw diets, energy and protein-based supplements are essential. This study showed that fiber-based supplements are just as, if not more, effective as starch-based supplements in rice straw utilization. This study shows that soybean hulls, in spite of their high fiber content, are as efficient as maize for supplementing rice straw primarily because fiber in soybean hulls is highly digestible as shown by in vivo digestibility and in situ disappearance.

• Asian-Australasian Journal of Animal Sciences
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• v.25 no.6
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• pp.800-805
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• 2012

Rice straw is an important roughage resource for ruminants in many rice-producing countries. In this study, a rice brittle mutant (BM, mutation in OsCesA4, encoding cellulose synthase) and its wild type (WT) were employed to investigate the effects of a cellulose synthase gene mutation on rice straw morphological fractions, chemical composition, stem histological structure and in situ digestibility. The morphological fractions investigation showed that BM had a higher leaf sheath proportion (43.70% vs 38.21%, p<0.01) and a lower leaf blade proportion (25.21% vs 32.14%, p<0.01) than WT. Chemical composition analysis showed that BM rice straw was significantly (p<0.01) higher in CP (crude protein), hemicellulose and acid insoluble ash (AIA) contents, but lower in dry matter (DM), acid detergent fiber (ADFom) and cellulose contents when compared to WT. No significant difference (p>0.05) was detected in neutral detergent fiber (NDFom) and ADL contents for both strains. Histological structure observation indicated that BM stems had fewer sclerenchyma cells and a thinner sclerenchyma cell wall than WT. The results of in situ digestion showed that BM had higher DM, NDFom, cellulose and hemicellulose disappearance at 24 or 48 h of incubation (p<0.05). The effective digestibility of BM rice straw DM and NDFom was greater than that of WT (31.4% vs 26.7% for DM, 29.1% vs 24.3% for NDFom, p<0.05), but the rate of digestion of the slowly digested fraction of BM rice straw DM and NDF was decreased. These results indicated that the mutation in the cellulose synthase gene could improve the nutritive value of rice straw for ruminants.

• Asian-Australasian Journal of Animal Sciences
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• v.10 no.2
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• pp.215-222
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• 1997

Ten rice varieties were planted at two locations (lowland and highland), during the wet and dry seasons of different years. In vivo digestibility and voluntary intake of the straw, were determined in groups of fat-tail sheep, supplemented with $18g{\cdot}kg^{-0.75}$ concentrate DM, containing ~20% crude protein. Voluntary intake of digestible straw organic matter (DOMI) consistently varied from 15.2 to $20.9g{\cdot}kg^{-0.75}$ between straw varieties, averaged over locations, years and seasons, despite considerable variation between individual batches. This variation in the nutritive value of the straw was independent of straw and grain yield, so it would seem that there is scope for selection of rice varieties with straw of higher nutritive value. The variation in DOMI of straw among location of growth, year and season, was of a magnitude similar to the improvement brought about by urea-ammoniation. The in sacco degradation characteristics and digestibility of rice straw residues were superior to those of the offered straw. This can be attributed to a preference for rice straw leaves relative to stems. Averaged over location of growth, year and season, characteristics of in sacco degradation, i.e. the rate of fermentative degradation and the truly undegradable fraction, emerged as accurate predictors of the nutritive value of rice straw.

• Magazine of the Korean Society of Agricultural Engineers
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• v.42 no.1
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• pp.92-98
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• 2000

This study is performed to evaluate the elastic properties of rice straw ash concrete using reices straw ash, cement, natural sand, gravel, and superplasticizer. The following conclusions are drawn ; The ultrasonic pulse velicity is in the range of 4,084 ~4,336m/s , which has showed about the same compared to that of the normla cement concrete. The highest ultrasonic pulse velocity is showed by 5 % rice straw ash filled rice straw ash concrete. The dynamic and static modulus of elasticity is in the range of 294 $\times$10$^3$ ~347 $\times$ 10$^3$ and 266 $\times$10$^3$~328 $\times$10$^3$kgf/㎤ , respectively. It is showed about the same compared to that of the normal cement concrete. The Poisson's number of rice straw ash concrete is less than that of the normal cement concrete.

• Proceedings of the Korean Society of Agricultural Engineers Conference
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• 1999.10c
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• pp.324-329
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• 1999

This study is performed to evaluate an elastic properties of rice straw ash concrete . The following conclusions are drawn ; The ultrasonic pulse velocity is in the range of 4.084 ∼4.336㎧, which has showed abuot the same compared to that of the normal cement concrete. The highest ultrasonic pulse velocity is showed by 5% rice straw ash filled reice straw ash concrete. The dynamic and static modulus of elasticity i sin the range of 294 ${\times}$103 ∼ 347 ${\times}$103 and 266${\times}$ 103 ∼347${\times}$ 103 kgf/$\textrm{cm}^2$ , respectively. It is showed about the same compared to that of the normla cement concrete. The poisson's number of rice straw ash concrete is less than that of the normal cement concrete . The stress-strain curve of concrete which is contained rice straw ash within 10% appear slowly and over 10% appear almost straightly.

• Korean Journal of Soil Science and Fertilizer
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• v.43 no.6
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• pp.1047-1050
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• 2010

This study was to investigate the effects of rice straw incorporation by cutting height on paddy soil fertility. The average residual amount of rice straw by cutting height were showed 1,420 kg $ha^{-1}$, 1,850 kg $ha^{-1}$, and 2,400 kg $ha^{-1}$ for depths of 10 cm, 15 cm, and 20 cm, respectively. For soil physical properties, soil hardness and bulk density were decreased while porosity was increased by rice straw incorporation. But soil organic matter (SOM), available silicate content, and cation exchange capacity (CEC) were significantly decreased when rice straw was removed from the field. These results indicated that the SOM as residual amount of rice straw was influenced by level of cutting height. Milled rice yield was increased by 28% and 32% for cutting heights of 15 cm and 20 cm, compared with that of control, respectively. The number of spikelets per square meter and the percentage of ripeness were increased with increasing incorporation by lower level of cutting height of rice straw. Therefore, incorporation of rice straw practices under cutting method influenced soil improvement and rice yield in paddy field.

• Journal of Microbiology and Biotechnology
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• v.27 no.3
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• pp.524-534
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• 2017

The development and utilization of crop straw biogas resources can effectively alleviate the shortage of energy, environmental pollution, and other issues. This study performed a continuous batch test at $35^{\circ}C$ to assess the methane production potential and volatile organic acid contents using the modified Gompertz equation. Illumina MiSeq platform sequencing, which is a sequencing method based on sequencing-by-synthesis, was used to compare the archaeal community diversity, and denaturing gradient gel electrophoresis (DGGE) was used to analyze the bacterial community diversity in rice straw, dry maize straw, silage maize straw, and tobacco straw. The results showed that cumulative gas production values for silage maize straw, rice straw, dry maize straw, and tobacco straw were 4,870, 4,032.5, 3,907.5, and $3,628.3ml/g{\cdot}VS$, respectively, after 24 days. Maximum daily gas production values of silage maize straw and rice straw were 1,025 and $904.17ml/g{\cdot}VS$, respectively, followed by tobacco straw and dry maize straw. The methane content of all four kinds of straws was > 60%, particularly that of silage maize straw, which peaked at 67.3%. Biogas production from the four kinds of straw was in the order silage maize straw > rice straw > dry maize straw > tobacco straw, and the values were 1,166.7, 1,048.4, 890, and $637.4ml/g{\cdot}VS$, respectively. The microbial community analysis showed that metabolism was mainly carried out by acetate-utilizing methanogens, and that Methanosarcina was the dominant archaeal genus in the four kinds of straw, and the DGGE bands belonged to the phyla Firmicutes, Bacteroidetes, and Chloroflexi. Silage maize is useful for biogas production because it contains four kinds of straw.

• Asian-Australasian Journal of Animal Sciences
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• v.9 no.2
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• pp.181-187
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• 1996

About 8 million tons of straw (dry matter) become rotten during the monsoon(June to August) season in Bangladesh. The possibility of preserving straw with urea, under wet condition during dry period (December to May) and its utilization by cattle has been studied. Five tons of fresh and wet(600g DM/kg material) rice straw were preserved for six months, with 5% urea(W/W) in either dome or rectangular shaped heap without any polythene cover. The preservation quality, acceptability and nutritive value of preserved straw were then comparted with that of dry straw in growing cattle. In both types of heaps, straw was excellently preserved having strong ammonia smell,dark brown in colour with no fungal infestation. Urea preservation of straw increased its CP and ADF content. Preserved straw was readily accepted by the animals and they were healthy throughout the experimental period. Compared to dry straw, urea preserved straw had nonsignificantly higher rumen degradability, straw intgake and growth rate. Similarly, digestibilities of DM(p<0.01), OM(p<0.01) & ADF(p<0.01) were significantly higher in the preserved than the dry straw. It was concluded that wet straw with relatively lower moisture(400 to 500 g/kg straw) content can be readily preserved by using urea without being covered with polythene. Whether the same phenomenon occurs in the preservation of fresh and weet rice straw with relatively higher moisture(600 to 700 g/kg straw) content is yet to be determined.