• Applied Biological Chemistry
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• v.15 no.3
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• pp.193-198
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• 1972

Twenty seven specimens of deteriolated storage rice were collected all over Korea. Types of deteriolation were classified according to color outlooks, and for 48 grains of each specimen the causative storage microorganisms were isolated and identified. The following results were observed; 1. 27 specimens of deteriolated rice were classified according to color outlooks into 7 types: reddish yellow 1, light reddish yellow 3, light grayish yellow 4, light red 6, dark gray 7, light gray 3, and rice weevil type 3. 2. The most common storage microorganisms group which infected deteriolated Korean rice were Aspergillus glaucus group, especially species of A. amstelodami, A. chevalieri, A. montevidensis, and A. ruber, which were frequently associated with Penicillium, Brevibactereum, and Bacillus. 3. As a specific case sometimes a specimen of deteriolated rice was infected chiefly by one deminant species of microorganism. Five cases were observed: that is, by P. islandicum, P. lanosum, B. lentus, Pseudomonas cohaerans, Brev. lipolyticum. 4. No definite relationship was observed between color outlook types and the deteriolation causing microorganisms. Only the heavily infected rice by Penicillium islandicum expressed discernible reddish yellow color indicative of the infection by this mold. 5. Mycotoxin problem could be noted in one specimen of deteriolated imported rice heavily infected by P. islandicum. Other mycotoxin producing fungi, A. flavus, A. ochraceus, A. fumigatus were also detected, but their growth frequencies were so low that it might not be serious problem.

• Korean journal of applied entomology
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• v.3
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• pp.27-30
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• 1964

The effects of the rice weevils(Sitophilus oryzae L.) on naked barley and wheat were studied in connection with the moisture contents and the molds in the grain under the controlled conditions; R.H. $75\%,\; 28^{\circ}C.$ 1. The moisture contents of control grain were decreased $2.07\%$ for naked barley and $1.29\%$ in wheat in four weeks. 2. The moisture contents of naked barley which had been infested with 100 weevils were decreased $0.29\%$ and were increased $0.79\% in the berley infested with 200 weevils at t beginning. In wheat, the moisture contents were decreased by$0.84\%\; and\; 0.13\%$ in respective experimental lots. 3. The moisture contents of grains have close relation with the population densities of the weevils in the grain. 4. The pattern of the change in the moisture content of grain have close relation with the population densities of the weevils in the grain. 5. The number of the mold colonies in the grain increased exponentially with the increase in the population densities of weevils in the grain. 6. The species of the mold found were A. restrictus and A. versicolor, which were the most abundant, and A. candidus was also found, but Ins common.

• Korean journal of applied entomology
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• v.46 no.1 s.145
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• pp.63-69
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• 2007

This study was conducted to examine the problems that would arise in our cropping systems when introducing Gain Threshold (GT) which is an important element in determining Economic Injury Level (EIL). Cain Threshold (GT) can be defined as the amount of damage (=yield loss) to justify management, and calculated by dividing the management costs by the market crop price. GTs for some pests in rice, soybean, and greenhouse vegetable crops cultivation were estimated and also compared with those in foreign countries. GTs and percentage of yield loss equivalent to them were estimated to be 4.6-6.1kg/10a/season and 1.0-1.3% for brown planthopper, white-backed planthopper, rice water weevil, and sheath blight, whereas for rice blast in rice cultivation were 12.7kg/10a/season and 2.7%. In soybean cultivation, the values for bean bug were 6.2 kg/10a/season and 3.6%. GTs and percentage of yield loss estimated for melon thrips, whitefly, and downy mildew in cucumber cultivation were 10.0-12.6kg/10a/week, 1.4-1.7%, and the values for two spotted mite and gray mildew in strawberry cultivation were 3.1-3.5kg/10a/week, 1.3-1.5%, and the values for American leaf minor, whitefly, and gray mold in tomato were 8.4-9.7kg/10a/week, 1.7-1.9%. Overall GTs in our cropping systems were so low compared to those in foreign countries, which meant that the low GTs might yield the low EILs. Therefore, we could suggest that prior to direct introduction of GTs calculated from current cultivation systems in developing EILs it is necessary to seriously consider reasonable values of GTs or the yield loss equivalents to them.

• Korean journal of applied entomology
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• v.20 no.2 s.47
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• pp.103-106
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• 1981

This experiment was carried out to investigate the vertical penetration and diffusion of fumigants for soybean disinfection in jumbo silo. The results were as follows, 1. Using the methylbromide independently as a soybean fumigant, penetration and diffusing velocity of methylbromide gas which moved from the top to the bottom through the vertica1 silo was too rapidly, it was possible to find out more 60mg/l of methylbromide gas concentration at bottom of silo within 4 hours after beginning the fumigation. And it showed the tendency of reducing methylbromide gas concentration gradually over the 10 hours from tile beginning the fumigation. 2. In case of added $CO_2$ gas to the methylbromide as a carrier is much more rapid velocity of penetration and diffusion of methylbromide gas than that of methylbromide gas independently. Therefore methylbromide gas concentration at bottom of silo was detected over the 70mg/l within 1.5 hours after beginning the fumigation.. 3. On the other hand, hence the phostoxin as a soybean fumigant was less velocity of Penetration and diffusion of the gas through the vertical silo compare to methylbromide gas, the phostoxin gas concentration couldn't detect over the 10mg/1 during the whole fumigation period at the bottomside of silo. 4. Test insects (rice weevil; sitophilus oryzae. L.) inserted at bottom of silo for examine the fumigation effect were killed completly by using the methylbromide independently and added $CO_2$ gas to methylbromide, while using the phostoxin the test insects were most alive.

• Journal of Conservation Science
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• v.30 no.2
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• pp.137-148
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• 2014

Large scale anoxic chamber system(volume $28m^3$) was developed and installed at The National Folk Museum of Korea for the first time in Korea. In order to get optimal anoxic treatment condition, we compared the disinfection of adults, larvae and eggs of cigarette beetles using nitrogen and argon. The time for complete disinfection of cigarette beetles in pine wooden blocks exposed to nitrogen at oxygen concentration 0.01% and 50% in relative humidity were 15 days at $20^{\circ}C$, 10 days at $25^{\circ}C$, and 7 days $30^{\circ}C$. Time were 10 days at $20^{\circ}C$, 7 days at $25^{\circ}C$, and 5 days $30^{\circ}C$ in argon anoxic atmosphere. From the mortality of cigarette beetles, optimal disinfection condition was oxygen concentration 0.01%, $25^{\circ}C$ in temperature, 50% in relative humidity and exposure time 21 days at nitrogen atmosphere. And when large scale anoxic chamber system was supplied nitrogen by nitrogen generator for anoxic treatment of many collections or large collections, it could be operated stably. To verify optimal disinfection condition, museum insects(adults, larvae, pupae and eggs of cigarette beetles in pine wooden blocks, cotton fabrics and Korean paper book, adults and larvae of drugstore beetles in pine wooden blocks, cotton fabrics and Korean paper book, larvae of varied carpet beetles in pine wooden block and silk fabrics, adults and larvae of hide beetles and adults of rice weevils in breeding boxes) which exposed at optimal disinfection condition, were completely killed.

• Korean journal of applied entomology
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• v.49 no.4
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• pp.375-384
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• 2010

We tried to establish the history of outbreaks and control methods of 'hwangchung' in Joseon Dynasty, uncovering the biological reality of the 'hwangchung' (called hwang or bihwang) analyzed through the Database program on 'the Annals of the Joseon Dynasty' and 'the Emollment of Haegoeje', two of the finest examples of classical historical records. The total number of articles on the outbreak of the hwangchung is 261 in the Annals of the Joseon Dynasty and 65 in the Emollment of Haegoeje. There were four peaks by hwangchung throughout the Joseon Era. Among them, the periods of King Taejo to King Sejong had the highest incidence. By comparing the number of records of the hwangchung from the Annals of the Joseon Dynasty with that in the Emollment of Haegoeje during the same period, results show the former was less than the latter, 35 vs 65. However, both records were relatively inconsistent with each other. Insect pests in forests as well as in agriculture were included in the biological identities of the hwangchung in the Joseon Dynasty periods, which is in accordance with those of Saigo's. The taxonomic identity could be confirmed in only 25 cases (9.5%) among the articles on hwangchung. It largely coincided with Paik's opinion: II in armyworm, nine in moth larva, one in rice stem borer, two in migratory locust, one in planthopper and one in rice-plant weevil. Therefore, it is not reasonable to regard hwangchung as a migratory locust or grasshopper only. The number of reports on the occurrence of hwangchung in the Annals of the Joseon Dynasty was 173 (66% of the total) and 47% of them were just simple reports, which means the report itself on the appearance of hwangchung was very significant. The reports on controlling insect pests were of low frequency, in 20% (34 cases) of the total reports, capturing insect pests or holding traditional ceremony called 'Poje'. Among them, only one case related to the treatment of seeds to prevent the damage by hwangchung was published in the King Sejong period. There were 37 discussions about changes and management of government policies due to disasters by hwangchung. They were mostly about relief or tax cut to the people who suffered damage and about cancellation of recruiting people to military training, constructing castles, and so on. It seems that not only the people but also the king was influenced by the hwangchung. In the case of King Seongjong, he referred to the stress of the prevention measure of locusts in 10 articles. The damage also had an effect on abdication in the reign of King Jeongjong.

• Korean journal of applied entomology
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• v.16 no.3 s.32
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• pp.171-179
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• 1977

Since the outbreak of the brown planthopper (Nilaparvata lugens) in 1915 caused tremendous losses in rice production, one of the more effective method of prevention of such a disaster could be the establishment of longterm forecasting system, In 1916 the author indicated there was a correlation between sunspot activities and brown planthopper and the white back planthopper outbreaks. However, the examples seem to be too small size to state a definite correlation. The purpose of the present study IS to revi~w the history of the brown planthopper outbreaks, and to establish a more effective forcasting system. The present forcasting methods are based on light trap catches of adults which already migrate into this country from mainland China. The regular cycle of 11.2 years of sunspot activity began in 1710, and was continued to present. To gather more records of brown planthopper, the author checked 'Joseon Wangjo Silrok' and analized the so-called 'Hwang' 'Hwang-chung' and 'Chung' which have multiple meanings, together with 'Samguk Sagi' 'Goryo Sa' and 'Munheon Bigo.' The results obtained by the about from review of these old literature citations revealed that ten species of insect and unknown species were involved: i. e., pine moth (Dendrolimus spectabilis), army worm (Mythimna separata), brown planthopper (Nilarvata lugens), white-back planthopper (Sogatella furcifera), migratory locust (Locutsa migratoria), rice stem borer (Chilo suppressalis,), mole cricket (Gryllotalpa africana), rice-plant weevil (Echinocnemus squameus), cut worm (Euxoa segetum), and mulberry pyralid Margaronia pyloalis) The suspected incidence of planthopper in old records expressed by 'Hwang' or 'Chung' revealed a total or 25 out of 37 in 'Samguk sagi,' 21 out of 49 in 'Goryo sa,' 9 of 73 in 'Wanjo-silrog,' and none of 8 in 'Munheon bigo' were planthoppers. Therefore, a total of 36 out of 167 records of insect incidence in the old literature can be possibly attributed to planthoppers. The brown planthopper and white-back planthopper migrate together to Korea every year from mainland China, However, the number of each species are differ by year. In 1975 outbreak the brown planthopper was dominant; and the white-back planthopper prevailed in 1946 and 1977 outbreaks, During the course of this study, the author was able to add a new record of outbreak of planthop per. In 1916 the white-back planthopper outbreak caused serious losses in Chungcheong-namdo and Jeonla-namdo, with losses estimated as high as 160 and 190 thousand seok (23.2 and 27.5 thousand M/T), in Naju and Secheon county, respectively. Since 1912, major outbreaks of brown planthopper or white-back planthopper have been recored 5 times. These occurrences coincide and well matched the period of minimum number of sunspots, With these authenticated records of planthoppers, the author believes there is a close correlation between brown planthopper and white-back planthopper outbreaks in Korea and sunspot activities. Therefore, in years of low number of sunspots, we should watch for and expect outbreaks of these. insects. At this time, it will be necessary to provide all possible prevention measures.

• Korean journal of applied entomology
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• v.12 no.1
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• pp.1-21
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• 1973

Some experiments were conducted to investigate the effects of the chemosterilant, hempa, on the biology of the rice weevil, Sitophilus oryzae L., and the transmission of the lethal factors in the progeny. One to three days old adult males were fed on the wheat grains treated with concentrations of 0.0625, 0.125, 0.25, and $0.5\%$ of hempa water solution. The effects of the treatment on the mortality, longevity, and the performance of oviposition were examined for the Pl generation, and the hatchability and mortality in the postembryonic development were also tested in the $F_1,\;F_2,\;BC_1,\;F_3,\;and\;BC_2$ generations to analyze the inheritance of the lethal factors. The results obtained were summarized as follows. (1) The average longevity of the treated males were ranged from 26.6 to 30.4 days, and indicated no statistical differences. (2) The mortality of the treated males were ranged between $3.3\%\;and\;13.3\%$ and showed no statistical significance. (3) The overall mean number of eggs laid by a female mated to a treated male with concentrations of 0.0625, 0.125, 0.26 and $0.5\%$ were 3.78, 4.05, 3.75 and 3.61 for the respective treatments, and they were not differ significantly from those of control which were 3.60 per female per 3 day period. The unmated female laid 1.91 in the same period, and significantly differ from those in other experimental groups. (4) The overall mean hatchability of the eggs laid by the females mated with males that had been treated with various concentrations of hempa were 86.82, 64.77, 53.47, 40.33 and $24.78\%$ for the respective concentrations of 0, 0.0625, 0.125, 0.25 and $0.5\%$. The hatchability decreased with the increasing concentrations. (5) The minimum hatchabilities were obtained from the eggs laid in the period of 10-12 days after treatment, then the hatchability increased showing some recovery. The recovery seemed to be very much delayed for the males which had been treated with the greater concentrations. Such a difference in hatchability might be related with the sensitivity of the developmental stages of the sperms, and broader spectrum in the stages and severer effects seemed to be associated with the increased concentrations. (6) The overall mean of larval mortality in the $F_l$ generation were 6.55, 17.89, 27.40, 35.42 and $52.17\%$ for the respective concentrations of 0,0.0625, 0.125,0.25 and $0.5\%$. And there was a tendency to increase in the mortality with the increase of concentrations. (7) The correlation coefficients between per cent sterile eggs and larval mortality for the experimental plots of 0.125, 0.25 and $0.5\%$ treatments showed r=+0.83 and +0.85, respectively, and it seemed to be close correlation between the lethal effects on the embryonic and post-embryonic developments. (8) Since the $SC_{50}$ of the sterile eggs was $0.133\%$ and $SC_{50}$ of the larval mortality was $0.565\%$, it was considered that tile lethal factors expressed more in the egg stages than the larval stages. (9) The ratio of female to male in the $F_l$ adults showed 100 : 125, 100 : 108 and 100 : 124 for the plots of 0.125, 0.25 and $0.5\%$ treatments, respectively. And it n·as considered that the sex ratio distortions might occur with the higher concentrations. (10) When the F, males originated 1.on the eggs had been laid by p, in the period of 16-18 days after treatment, were crossed to normal females $(BC_1)$ and made sib matings $(F_2)$, the per cent sterile eggs of the $BC_1$ generation were 13.88 and $33.04\%$ , and were 31.01 and $38.73\%$ for the $F_2$generation with the plots of 0.0625 and $0.125\%$ treatment, respectively. And these seemed to be a results of the $F_1$ individuals are carrying some chromosomal aberrations (11) The larval mortality was the highest in the $F_2$ plot and followed the female backcross plot, and the least in the male backcrosses. (12) The proportions of 1st and 2nd instar larvae among the larval development at tile 17th day after oviposition were 10.98, 27.26, 32.98 and $15.73\%$ in the normal female $\times$ normal male, $F_1$ female$\times$normal male, normal $female \;\times F_1$ male and $female \;\times F_1$ male plots, respectively. It was considered that the larval development might be delayed by the treatment in the 2nd generation. (13) Per cent larval mortality and sterile eggs were greater in the $F_2$ sib mating plots $(F_3)$ than both of $F_2$ backcrosses. Therefore, it seemed that some of the recessive lethal mutations might affect in the further generations. (14) The sterility, induced by the treatment of chemosterilant, hempa, was considered as the result of the dominant lethal mutations due to chromosomal aberrations such as translocation and/or deletion. The effects of these lethal factors seemed to be inherited tip to 3rd generation after treatment.

• Korean journal of applied entomology
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• v.20 no.4 s.49
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• pp.212-216
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• 1981

This experiment was carried out to investigate the penetration velocity and diffusion ability of methyl bromide fumigants under the condition of natural gravity, and the effect of in control of yellow corn in the vortical silo. The results were as fallows 1. The methyl bromide remainded concentration on the surface of the vertical silo was rapidly reduced from over 100mg/l to under 20mg/l, within 2 hours after supplying methyl bromide fumigant 2. Even 3m depth place from the surface of the vertical silo filled with yellolw corn was well penetrated within 30 minutes, but its remained methyl bromide gas was reduced gradually from over 100mg/l to 30mg/l after 12 hours duration. 5. In case of 13m place tile penetration ability of methyl bromide was over 100mg/l within one hour and kept the same concentration of it for 12 hours, its remainded methyl bromide preserved over 40mg/l until 48 duration time. 4. Less methyl bromide gas was detected at the bottom place than 3m and 13m depth of the vertical silo. It showed bellow 20mg/l of methyl bromide gas only through the whole duration time. 5. Comparing to the vertical penetration velocity of mehtyl bromide gas, the horizontal diffusion ability was not so good. Therefore, remained methyl bromide gas of 3m depth at the wall side of silo was about half than that of center circle where the nearer place from the methyl bromide supplying point, and the methyl bromide gas of 13m depth placed center circle was detected about 15 to 20 times than that of wall side. 6. For the purpose of examining the mortality of methyl bromide, the testing insects (Lesser Rice weevil and confused flour beetle) placed on the surface and bottom side of the silo were killed completely after 24 hrs fumigation, and the same results showed also at the 3m, 7m and 13m depth after 48 hrs fumigation.