• The Plant Pathology Journal
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• v.27 no.4
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• pp.310-314
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• 2011

Nonstructural protein 3 (NS3) encoded by RNA3 of Rice stripe virus (RSV), known to be a suppressor of gene silencing, was cloned and sequenced. The cloned NS3 gene is composed of 636 nucleotides encoding 211 deduced amino acids, and showed a high degree of similarity with the equivalent genes isolated from Korea, Japan and China. The NS3 gene promoted the enhancement of transient gene expression and suppressed transgene co-silencing. In the transient GFP expression via agroinfiltration, GFP expression was dramatically enhanced in terms of both protein yield and expression period in the presence of NS3. The highest accumulation of GFP protein reached to 6.8% of total soluble proteins, which corresponded to a two-fold increase compared to that obtained in the absence of NS3. In addition, NS3 significantly suppressed the initiation of GFP co-silencing induced by the additive GFP infiltration in GFP-transgenic Nicotiana benthamiana. The NS3 gene was also found to be a stronger suppressor than Cucumber mosaic virus 2b. These observations are believed to be derived from the strong suppressive effect of NS3 on gene silencing, and indicate that NS3 could be used as an effective enhancer for the rapid production of foreign proteins in plants.

• Korean journal of applied entomology
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• v.16 no.4 s.33
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• pp.217-220
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• 1977

During 1976 the effects of carbofuran placement in the root zone of the rice plants, the varieties Palkweng and Yushin, were evaluated for control of common rice pests in the paddy field at the Honam Crops Experiment Station. The methods of insecticide placement included the use of capsule formulation and liquid injection by the root-zone liquid insecticide injector designed at the International Rice Research Institute. The single ro~t-zone application of carbofuran at 2 days after transplanting was compared with two and fcur broadcast applications of carbofuran and diazinon. Capsules were the most. effective in controlling the striped rice borer (Chilo suppressalis), small brown plant-hopper (Laodelphax striatellus), green rice leafhopper (Nephotettix cincticeps) and stripe virus disease which is transmitted by the small brown planthopper. However, one injector application of carbofuran was equal or better the broadcast applications. Their control effectiveness were more significant on Palkweng susceptible to common rice pests than on Yushin resistant to the stripe virus disease.

• Korean journal of applied entomology
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• v.14 no.3 s.24
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• pp.147-153
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• 1975

During 1973 a field experiment was carried out to evaluate effectiveness of the root zone application of insecticides in medical gelatin capsules for control of several rice insect pests and dwarf virus disease. At three days after transplanting the capsules were pushed by hand about 2.5cm into the soil, near roots of Tongil rice plants. At the given day intervals number of leaf-and plant-hoppers on the hills were recorded by direct count, and dead hearts and white heads by stem borers and dwarf virus infected hills were observed in the experiment plots. Finally grain yields were measured. The percentages of dead hearts and white heads in all the plots were too low for evaluating the effectiveness of insecticides against the striped rice borers. Carbofuran of the insecticides tested was relatively effective against green rice leafhopper (Nephotettixcincticeps) and small brown planthopper (Laodelphax striatellus), although small number of insects on the hills were recorded. Relatively small number of white-backed planthopper (Sogatella furcifera) was occured in the BPMC and Diazinon treated plots. BPMC and Carbofuran were highly effective against the brown planthopper (Nilaparvata lugens) to 80 days after treatment. Incidence of dwarf virus disease was least in the plots of Carbofuran treatment. The highest grain yield was recorded in the plots treated with Carbofuran, and it seemed to be related with good protection from the dwarf virus disease and leaf-and plant-hoppers.