• 한국유기농업학회지
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• 제5권2호
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• pp.117-128
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• 1997

This study was carried out to investigate the amounts of voluntary intake, digestibility and nitrogen retention in Korean native goats (KNG) fed agricultural by-products containing rice straw (RS) and apple pomace (AP) and to obtain a basic information for establishing the feeding system of KNG. The result are as follows. 1. Among the chemical composition of experimental diets, the highest values in crude protein (CP ; 18.6%) and crude ash contents (10.7%) were observed in alfalfa hay. Those of RS+AP treatment were significantly low 6.0 and 4.8%, respecitively. Acid detergent fiber (ADF), ether extract and non-structural carbohydrate (NSC) contents were shown an adverse tendency. 2. Dry matter (DM) intakes per day in KNG fed RS+commercial diet (CD), RS+AP and RS+AP+CD were significantly lower (P<0.05) 210.3, 228.3, respectively than 358.1g in alfalfa hay. 3. DM intakes per basal weight expressed as DM g/kg of BW0.75 and DM g/kg of BW(%) were highest (P<0.05) 60.5g and 3.3%, respectively in KNG fed alfalfa hay, any other treatments showed 35.6 to 42.5g and 2.0 to 2.3%, respectively and this result was similar to those of DM intake per day. 4. Digestibilities of DM, organic matter, CP, ADF, neutral detergent fiber (NDF). crude ash and ether extract in alfalfa hay and RS+AP+CD treatment were significantly higher (P<0.05) than RS+CD treatment. Those of CP, ADF and NDF of RS+AP treatment in not significantly different with these treatments. 5. NSC digestibility was higher in alfalfa hay (66.7%) than those of other treatments (44.2~52.0% ; P<0.05). 6. Nitrogen retentions expressed as N retention(g) and N retention (%) in KNG were highest (P<0.05) 0.6g and 15.6%, respectively in RS+AP+CD treatment and RS+CD treatment was lowest (P<0.05) -06g and -21.4%, respectively.

• Asian-Australasian Journal of Animal Sciences
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• 제17권11호
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• pp.1535-1540
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• 2004

Six dry Holstein cows were used to evaluate the effect of dietary neutral detergent fiber (NDF) concentration and particle size (PS) on chewing activity. Treatments were arranged in a 3$\times$3 factorial design; total mixed rations contained three NDF concentrations (26, 32, 38%) and three PS (1.0, 1.5, 2.0 cm). NDF levels and particle sizes of diets were adjusted by formulating rate and cutting length of alfalfa hay and rice straw. Cows were fed twice daily at 90% of ad libitum feed intake throughout the experiment. Chewing activity was positively associated with NDF concentration, but not significantly affected by PS of diet. Eating time per unit of NDF intake was affected by PS rather than NDF concentration of diet. Time spent ruminating per unit DM or NDF intake increased with increasing NDF concentration of diet, but was not affected by PS. As the PS of diet increased, the eating time per day increased, but the rumination time decreased. In addition, as the number of rumination bolues decreased the rumination duration increased as well as the chews per bolus. The regression equation induced from relationships of NDF concentrations (NDF, %) and particle sizes (PS, cm) of diet on roughage value index (RVI, min of chewing time/kg DMI) was as follows. RVI=-19.672+1.44$\times$NDF+5.196$\times$PS, ($R^{2}$=0.81).

• Asian-Australasian Journal of Animal Sciences
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• 제29권11호
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• pp.1601-1607
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• 2016

This study was conducted to determine the effect of lysozyme addition on in vitro rumen fermentation and to identify the lysozyme inclusion rate for abating methane ($CH_4$) production. An in vitro ruminal fermentation technique was done using a commercial concentrate to rice straw ratio of 8:2 as substrate. The following treatments were applied wherein lysozyme was added into 1 mg dry matter substrate at different levels of inclusion: Without lysozyme, 2,000, 4,000, and 8,000 U lysozyme. Results revealed that, lysozyme addition had a significant effect on pH after 24 h of incubation, with the highest pH (p<0.01) observed in 8,000 U lysozyme, followed by the 4,000 U, 2,000 U, and without lysozyme. The highest amounts of acetic acid, propionic acid (p<0.01) and total volatile fatty acid (TVFA) (p<0.05) were found in 8,000 U after 24 h of incubation. The $CH_4$ concentration was the lowest in the 8,000 U and the highest in the without lysozyme addition after 24 h of incubation. There was no significant differences in general bacteria, methanogen, or protozoan DNA copy number. So far, addition of lysozyme increased the acetate, propionate, TVFA, and decreased $CH_4$ concentration. These results suggest that lysozyme supplementation may improve in vitro rumen fermentation and reduce $CH_4$ emission.

• Animal Bioscience
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• 제36권4호
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• pp.601-608
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• 2023

Objective: This study was conducted to investigate the effects of energy and protein levels in the diet of Hanwoo heifers on growth response and animal behavior. Methods: Forty heifers were randomly allocated into three experimental groups according to the target daily weight gain in 8 pens (T-0.2, 2 replications; T-0.4 and -0.6, 3 replications) based on similar body weight (BW) and age in months. The target average daily gain (ADG) was set at 0.2 (T-0.2), 0.4 (T-0.4), and 0.6 kg/d (T-0.6), and feed was based on National Institute of Animal Science (NIAS, 2017). In order to minimize hunger stress of T-0.2 and -0.4, the feeding ratio of rice straw was set to 55%, 50%, and 45% for T-0.2, -0.4 and T-0.6, respectively, so that the dry matter (DM) intake for all treatment groups was uniform but the energy and protein levels in the diet were adjusted differently. A total of 6 items (lying, standing, eating, rumination, walking and drinking) of animal behavior were analyzed. Results: During the whole period of the experiment, the ADG of the T-0.2, -0.4 and -0.6 treatments were 0.48, 0.56, and 0.65 kg/d (p<0.05), respectively, showing higher gain than the predicted value, especially for the low target ADG group. Based on these results, regression equations for the total digestible nutrient (TDN) and crude protein (CP) requirements were derived. No behavioral differences were found according to the energy and protein levels in the diet because the DM intake was kept constant by adjusting the roughage and concentration ratio. However, eating time was longer (p<0.05) at T-0.2 than T-0.6 during the whole day. Conclusion: Through this study, it was possible to derive regression equations for predicting TDN and CP requirements according to the target ADG and BW.

• Journal of Animal Science and Technology
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• 제47권6호
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• pp.963-974
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• 2005

본 시험은 두충 급여가 거세한우의 성장, 도체특성, 육질특성 및 육의 지방산 조성에 미치는 영향을 구명하기 위하여 수행하였다. 기초사료로 배합사료와 볏짚을 급여하였으며, 건조 두충 잎을 건물기준으로 조사료의 3, 5 및 10%를 대체․급여하였다. 시험사료의 사료적 가치를 평가하기 위하여 평균체중 321±13kg의 거세한우 72두(4개구$\time$6 두$\time$3 반복)를 공시하여 약 15개월간 실시하였다. 일당 증체량은 대조구를 포함한 모든 처리구에서 0.81-0.86kg으로 유의차는 없었다. 사료요구율도 대조구를 포함한 모든 처리구에서 10.05-10.59로서 개선효과는 없었다. 분변내 NH3 가스 발생량은 두충 잎의 급여비율이 높을수록 감소하였다. 즉 40℃에서 12시간 발효시에는 대조구와 3% 구는 각각 30.6ppm과 29.8ppm이었으나 5% 구와 10% 구는 각각 20.3ppm과 21.6ppm으로 낮았다(p<0.05). 등지방 두께는 대조구는 15.0mm였으며 5-10% 급여시 10.2-10.5mm로 얇은 경향이었다(p<0.05). 육량 A 등급 출현율은 3% 급여시 약 20%가 증가하였으며, 육질 1+와 1 등급 출현율은 두충 잎을 5-10% 급여시 약 10-15%가 증가하였다. 등심의 육색에 대한 CIE L*, a* 및 b* 값은 각 시험구간 차이는 없었으며, 우둔의 CIE L*, a* 및 b* 값에서도 각 시험구간에 차이는 없었다. 지방산 함량에 있어서 등심과 우둔에서 공히 C18:1은 두충 잎을 5-10% 급여구에서 증가하였다(p<0.05). 등심의 cholesterol 함량은 10% 급여한 구가 53.3mg /100g으로 대조구와 3% 구 및 5% 구의 55.7-57.0mg/100g으로 비해 낮았다(p<0.05). 하리의 발생비율은 대조구가 약 20.3%였으며 두충 잎을 5-10% 대체 급여할 경우에는 10.4-12.5%로 크게 감소되었다(p<0.05). 호흡기 발병비율은 두충 잎을 대체함으로 개선되는 경향이지만 유의차는 없었다. 이상의 결과를 종합하면, 두충 잎에는 다양한 기능성을 가지는 생리활성 물질이 상당량 함유된 기능성 사료로의 개발 가능성이 매우 높다. 또한 두충 잎을 음건하여 반추동물에 급여할 경우 육질 개선 및 하리 발생비율이 감소되기 때문에 고급육 생산에 효과적이었다. 따라서 두충 잎을 거세한우에 급여할 경우 조사료의 5-10%를 대체하는 것이 가장 유리할 것으로 생각된다.

• 농업과학연구
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• 제27권1호
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• pp.25-32
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• 2000

Monensin의 첨가 급여가 한우의 육생산 효율 및 제1위내 발효성상에 미치는 효과를 구명하고자 본 실험을 실시하였다. 거세우 72두(평균체중 266.9kg)를 0, 22, 및 33 ppm의 monensm 처리구에 처리당 3반복, 반복당 8두씩 체중이 비슷하도록 배치하여 옥외 사육장에서 140일간 사양시험을 실시하였다. Monensin은 농후사료에 0, 22, 및 33 ppm 수준으로 첨가하였으며, 농후사료와 조사료(15cm 정도로 절단한 볏짚)는 자유채식 시켰다. 본 실험에서 얻어진 결과는 다음과 같다. 1. 일당증체량은 각 처리구 간에 유의한 차이를 나타내지 않았다. 2. 총사료(농후사료+조사료)섭취량은 처리구간에 유의성은 없었으나 monensin 급여수준의 증가와 함께 감소하는 경향이어서 33ppm구에서 약 5% 의 감소를 나타내었다. 3. 총사료효율(총사료섭취량/증체량)에 있어서는 처리구간에 유의성은 없었으나 0ppm 구에 비하여 22ppm구와 33ppm구가 각각 5.2%와 5.1%의 거의 통일한 개선을 나타냈다. 4. 제1위내 total VFA와 acetic acid의 농도는 monensin에 의한 일정한 경향이 없었고, propionic acid 농도는 처리간에 유의성은 없었으나 무첨가구에 비하여 22 및 33ppm 구에서 각각 13.8%와 19.3%의 증가를 보였다. Butyric acid 농도는 22ppm구에서도 감소하는 경향이었지만, 33ppm구에서는 유의한 ($p{\leq}0.054$) 감소가 인정되었다. A/P비는 monensin 무첨가구에 비하여 22ppm구와 33ppm구에서 각각 12.5 %의 감소를 나타내었다. 결론적으로 monensin은 propionic acid 의 증가와 butyric acid 의 감소 및 A/P비의 감소를 가져오는 효과와 함께 사료섭취량을 감소시켜서 비육우의 사료효율을 개선하는 효과가 있었다. 따라서 monensin은 축우의 비육시 육생산 효율을 높이기 위한 사료첨가제로서 충분한 사용가치가 있다고 사료되며, 22ppm과 33ppm 수준이 거의 통일한 사료효율을 보인 것으로 보아 22ppm 수준에서도 기대하는 효과를 얻을 수 있을 것으로 생각된다.

• Asian-Australasian Journal of Animal Sciences
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• 제28권10호
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• pp.1454-1464
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• 2015

Four methods were adopted, including the mobile nylon bag (MNB) method, modified three-step in vitro (MTS) method, original three-step in vitro (OTS) method, and acid detergent insoluble nitrogen (ADIN) estimating method, to evaluate the intestinal digestibility of rumen undegradable protein (DRUP) of 10 types of concentrates and 7 types of roughages. After correlation analysis to determine the DRUP values using the MNB, MTS, OTS, and ADIN methods, the study aimed to find out appropriate methods to replace the MNB method due to its disadvantages such as high price, long time period, and use of a duodenal T-fistula. Three dairy cows with a permanent ruminal fistula and duodenal T-fistula were used in a single-factor experimental design. The results showed that the determined DRUP values using the MNB method for soybean meal, cottonseed meal, rapeseed meal, sunflower meal, corn germ meal, corn, rice bran, barley, wheat bran, corn fiber feed, Alfalfa (Zhao dong), Alfalfa (Long mu 801), Alfalfa (Long mu 803), grass (North), Grass (Inner Mongolia), corn silage and corn straw were 98.13%, 87.37%, 88.47%, 82.60%, 75.40%, 93.23%, 69.27%, 91.27%, 72.37%, 79.03%, 66.72%, 68.64%, 73.57%, 50.47%, 51.52%, 54.05%, and 43.84%, respectively. The coefficient of determination ($R^2=0.964$) of the results between the MTS method and the MNB method was higher than that ($R^2=0.942$) between the OTS method and the MNB method. The coefficient of determination of the DRUP values of the concentrates among the in vitro method (including the MTS and OTS methods) and the MNB method was higher than that of the roughage. There was a weak correlation between the determined DRUP values in concentrates obtained from the ADIN method and those from the MNB method, and there was a significant correlation (p<0.01) between the determined DRUP values of the roughage obtained from the MNB method and those obtained from ADIN method. The DRUP values were significantly correlated with the nutritional ingredients of the feeds. The regression equation was DRUP =100.5566+0.4169CP - 0.4344SP - 0.7102NDF - 0.7950EE ($R^2=0.8668$, p<0.01; CP, crude protein; SP, soluble protein; NDF, neutral detergent fiber; EE, ether extract). It was concluded that both the MTS method and the OTS may suitable to replace the MNB method for determining the DRUP values and the former method was more effective. Only the ADIN method could be used to predict the values of the roughages but conventional nutritional ingredients were available for all of the samples' DRUP.

• 농업과학연구
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• 제8권2호
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• pp.164-170
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• 1981

젖소등 초식 가축의 사료 자원개발을 위하여 국내 산지에 다량으로 자생되고 있는 관목류 및 초류중 비교적 가축 사료로써 활용 가치가 있어 보이는 수종의 관목초류를 택하여 시기별로 사료성분을 분석하고 젖소에 대한 일부 급여 시험을 실시하여 다음과 같은 결론을 얻었다. 1. 관목초류의 건물 함량은 평균 25~45%의 범위를 나타내고 있으며 이중 청미례와 댕댕이덩쿨이 각각 26.25%와 26.36%로 가장 낮았고 졸참나무가 47.19%로 가장 높게 나타났다. 2. 조단백질 함량은 건물량 기준으로 대부분 10% 이상으로 나타났고 이중 칙과 자귀나무가 평균 20% 정도로 가장 높게 나타났으며 억새와 새는 평균 10% 미만으로 낮게 나타났다. 3. 조섬유 함량은 평균 20~30% 뱅위로 타나났으며 개암나무와 청미례가 20% 정도로 가장 낮았고 억새와 새는 37% 이상으로 높게 나타났다. 4. 대부분 관옥 사초류는 시기가 경과함에 따라 조단백질 함량이 줄어 들고, 조섬유 함량이 증가되는 경향을 보였으며, 또한 건물량이 증가되였다. 5. 관목초본류를 볏짚 대용으로 1일 두당 5kg을 급여한 결과 체중과 유량 및 무지고형분 함량이 약간 증가되는 경향을 보였다.

• Asian-Australasian Journal of Animal Sciences
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• 제25권7호
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• pp.971-979
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• 2012

Four, lactating dairy crossbreds ($50%{\times}50%$ Holstein Friesian${\times}$Native Zebu cattle) were randomly assigned according to a $2{\times}2$ factorial arrangement (two protein levels and two levels of mangosteen peel pellets (Mago-pel)) in a $4{\times}4$ Latin square design to receive four dietary treatments. All cows received concentrate at a proportion of 1 kg concentrate per 2 kg of milk yield, and urea-treated 5% rice straw (UTRS) was given ad libitum. It was found that total dry matter intakes, nutrient digestibility, ruminal pH and $NH_3$-N concentrations were not affected (p>0.05) by treatments. Concentrations of ruminal pH and $NH_3$-N were not affected by dietary treatments although the concentration of BUN varied significantly (p<0.05) between protein levels (p<0.05). The populations of rumen bacteria and fungal zoospores did not differ among treatments (p>0.05); however, the population of protozoa was decreased (p<0.05) when cows received Mago-pel supplementation. The composition of the population of bacteria, identified by real-time PCR technique, including total bacteria, methanogens, Fibrobacter succinogenes and Ruminococcus albus was similar (p>0.05) among dietary treatments (p>0.05); however, copy numbers of Ruminococcus flavefaciens was increased when protein level increased (p<0.05). Microbial protein synthesis, in terms of both quantity and efficiency, was enriched by Mago-pel supplementation. Milk yield was greatest in cows fed UTRS based diets with concentrate containing protein at 16% CP with Mago-pel, but were lowest without Mago-pel (p<0.05). In addition, protein level and supplementation of Mago-pel did not affect (p>0.05) milk composition except solids-not-fat which was higher in cows fed the diet with 19% CP. Therefore, feeding a concentrate containing 16% CP together with 300 g/hd/d Mago-pel supplementation results in changes in rumen fermentation and microbial population and improvements in milk production in lactating dairy crossbreds fed on UTRS.

• Journal of Animal Science and Technology
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• 제53권6호
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• pp.533-540
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• 2011

The objectives of experiments were to compare directly the effects of synchrony of slowly fermented (corn and corn gluten meal; C-CGM) and of rapidly fermented ingredients (barley and soybean meal; B-SBM) in the rumen on starch disappearances in gastrointestinal tracts (Experiment 1) and growth performance (Experiment 2) of Hanwoo steers in the feedlot barn. In experiment 1, four Hanwoo steers ($288{\pm}21$ kg) fitted with ruminal and "T" shaped duodenal cannula were placed in one pen with Calan gate and assigned randomly to a duplicate $2{\times}2$ Latin square design. In experiment 2, eight intact Hanwoo steers ($311{\pm}8$ kg) were assigned randomly to one of two pens with Calan gate to evaluate the effect of the same diets as like in experiment 1 on growth performance. There were no differences in ruminal pH, ammonia and total VFA concentrations between treatments. Percentage of apparent ruminal starch disappearance was 33.3% unit lower (p<0.05) for steers fed C-CGM than for steers fed B-SBM diets and this difference resulted in 268% higher (p<0.05) in duodenal starch flows for steers fed C-CGM diet than for steers fed B-SMB diet. There was significant increase (p<0.05) in quantity (927 vs. 400 g/d) of corn starch digested post-ruminally compared to barley starch. However, percentage of starch apparently digested post-ruminally was 8% higher (p=0.1) in steers fed fast synchrony diet with B-SBM than in steers fed slow synchrony diets with C-CGM. The differences of percentage and amount of starch apparently digested post-ruminally between C-CGM and B-SBM diets did not affect rice straw DM intake, average daily gain (ADG) and feed efficiency. In conclusion, there is some uncertainty in regards to the relationship between site of starch digestion and DM intake, ADG, and feed efficiency in this study.